pYBA-1132
$99.00
In stock
SKU
PL83588
KF876796.1pYBA1132
100μg liquid plasmid(Instantly):$120
| name | pYBA-1132 | another name | apYBA-1132 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | 35S | Replicon | pBBR1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pYBA-1132 | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTGGGATGAA TGTCAGCTAC TGGGCTATCT GGACAAGGGA AAACGCAAGC GCAAAGAGAA AGCAGGTAGC TTGCAGTGGG CTTACATGGC GATAGCTAGA CTGGGCGGTT TTATGGACAG CAAGCGAACC GGAATTGCCA GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT GGCTTTCTTG CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTTCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCCATGGG CAAATCGTGG CAGGATATAT TGTGGTGTAA ACACGCGTCA CGTGGCGCGC CGATCATGAG CGGAGAATTA AGGGAGTCAC GTTATGACCC CCGCCGATGA CGCGGGACAA GCCGTTTTAC GTTTGGAACT GACAGAACCG CAACGTTGAA GGAGCCACTC AGCCGCGGGT TTCTGGAGTT TAATGAGCTA AGCACATACG TCAGAAACCA TTATTGCGCG TTCAAAAGTC GCCTAAGGTC ACTATCAGCT AGCAAATATT TCTTGTCAAA AATGCTCCAC TGACGTTCCA TAAATTCCCC TCGGTATCCA ATTAGAGTCT CATATTCACT CTCAATCCAA ATAATCTGCA CCGGATCTAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTTCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGATCGTT CAAACATTTG GCAATAAAGT TTCTTAAGAT TGAATCCTGT TGCCGGTCTT GCGATGATTA TCATATAATT TCTGTTGAAT TACGTTAAGC ATGTAATAAT TAACATGTAA TGCATGACGT TATTTATGAG ATGGGTTTTT ATGATTAGAG TCCCGCAATT ATACATTTAA TACGCGATAG AAAACAAAAT ATAGCGCGCA AACTAGGATA AATTATCGCG CGCGGTGTCA TCTATGTTAC TAGATCGGGA TTAATAGGCC TCGTACGACC GGTAACATGG TGGAGCACGA CACTCTGGTC TACTCCAAAA ATGTCAAAGA TACAGTCTCA GAAGACCAAA GGGCTATTGA GACTTTTCAA CAAAGGATAA TTTCGGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTCATC GAAAGGACAG TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCTATCATTC AAGATCTCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAC ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA GTTCATTTCA TTTGGAGAGG ACAATTGGAG CTCCACCGCG GTGGCGGCCG CTCTAGAACT AGTGGATCCC CCGGGCTGCA GGAATTCGAT ATCAAGCTTA TCGATACCGT CGACCTCGAG GGGGGGCCCG GTACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCGCGATG ACTAACTAGC AAATCACCAG TCTCTCTCTA CAAATCTATC TCTCTCTATT TTTCTCCAGA ATAATGTGTG AGTAGTTCCC AGATAAGGGA ATTAGGGTTC TTATAGGGTT TCGCTCATGT GTTGAGCATA TAAGAAACCC TTAGTATGTA TTTGTATTTG TAAAATACTT CTATCAATAA AATTTCTAAT TCCTAAAACC AAAATCCAGT TTAAACGCGT TGACAGGATA TATTGGCGGG TAAACTAAGT CGCTGTATGT GTTTGTTTGT ATTCTGCCTC CCAGAGCCTG ATAAAAACGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGAGGCGGC TACAGCCGAT AGTCTGGAAC AGCGCACTTA CGGGTTGCTG CGCAACCCAA GTGCTACCGG CGCGGCAGCG TGACCCGTGT CGGCGGCTCC AACGGCTCGC CATCGTCCAG AAAACACGGC TCATCGGGCA TCGGCAGGCG CTGCTGCCCG CGCCGTTCCC ATTCCTCCGT TTCGGTCAAG GCTGGCAGGT CTGGTTCCAT GCCCGGAATG CCGGGCTGGC TGGGCGGCTC CTCGCCGGGG CCGGTCGGTA GTTGCTGCTC GCCCGGATAC AGGGTCGGGA TGCGGCGCAG GTCGCCATGC CCCAACAGCG ATTCGTCCTG GTCGTCGTGA TCAACCACCA CGGCGGCACT GAACACCGAC AGGCGCAACT GGTCGCGGGG CTGGCCCCAC GCCACGCGGT CATTGACCAC GTAGGCCGAC ACGGTGCCGG GGCCGTTGAG CTTCACGACG GAGATCCAGC GCTCGGCCAC CAAGTCCTTG ACTGCGTATT GGACCGTCCG CAAAGAACGT CCGATGAGCT TGGAAAGTGT CTTCTGGCTG ACCACCACGG CGTTCTGGTG GCCCATCTGC GCCACGAGGT GATGCAGCAG CATTGCCGCC GTGGGTTTCC TCGCAATAAG CCCGGCCCAC GCCTCATGCG CTTTGCGTTC CGTTTGCACC CAGTGACCGG GCTTGTTCTT GGCTTGAATG CCGATTTCTC TGGACTGCGT GGCCATGCTT ATCTCCATGC GGTAGGGTGC CGCACGGTTG CGGCACCATG CGCAATCAGC TGCAACTTTT CGGCAGCGCG ACAACAATTA TGCGTTGCGT AAAAGTGGCA GTCAATTACA GATTTTCTTT AACCTACGCA ATGAGCTATT GCGGGGGGTG CCGCAATGAG CTGTTGCGTA CCCCCCTTTT TTAAGTTGTT GATTTTTAAG TCTTTCGCAT TTCGCCCTAT ATCTAGTTCT TTGGTGCCCA AAGAAGGGCA CCCCTGCGGG GTTCCCCCAC GCCTTCGGCG CGGCTCCCCC TCCGGCAAAA AGTGGCCCCT CCGGGGCTTG TTGATCGACT GCGCGGCCTT CGGCCTTGCC CAAGGTGGCG CTGCCCCCTT GGAACCCCCG CACTCGCCGC CGTGAGGCTC GGGGGGCAGG CGGGCGGGCT TCGCCTTCGA CTGCCCCCAC TCGCATAGGC TTGGGTCGTT CCAGGCGCGT CAAGGCCAAG CCGCTGCGCG GTCGCTGCGC GAGCCTTGAC CCGCCTTCCA CTTGGTGTCC AACCGGCAAG CGAAGCGCGC AGGCCGCAGG CCGGAGGCTT TTCCCCAGAG AAAATTAAAA AAATTGATGG GGCAAGGCCG CAGGCCGCGC AGTTGGAGCC GGTGGGTATG TGGTCGAAGG CTGGGTAGCC GGTGGGCAAT CCCTGTGGTC AAGCTCGTGG GCAGGCGCAG CCTGTCCATC AGCTTGTCCA GCAGGGTTGT CCACGGGCCG AGCGAAGCGA GCCAGCCGGT GGCCGCTCGC GGCCATCGTC CACATATCCA CGGGCTGGCA AGGGAGCGCA GCGACCGCGC AGGGCGAAGC CCGGAGAGCA AGCCCGTAGG GCGCCGCAGC CGCCGTAGGC GGTCACGACT TTGCGAAGCA AAGTCTAGTG AGTATACTCA AGCTAGCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAA
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