pSilent-1
$55.00
In stock
SKU
PL83545
DB00081;
100μg liquid plasmid(Instantly):$57
| name | pSilent-1 | another name | apSilent-1 |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Interfere with silence | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | TrpC | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | T7-R：CCCTATAGTGAGTCGTATTA | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCGCGC AATTAACCCT CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCGACGT TAACTGATAT TGAAGGAGCA CTTTTTGGGC TTGGCTGGAG CTAGTGGAGG TCAACAATGA ATGCCTATTT TGGTTTAGTC GTCCAGGCGG TGAGCACAAA ATTTGTGTCG TTTGACAAGA TGGTTCATTT AGGCAACTGG TCAGATCAGC CCCACTTGTA GCAGTAGCGG CGGCGCTCGA AGTGTGACTC TTATTAGCAG ACAGGAACGA GGACATTATT ATCATCTGCT GCTTGGTGCA CGATAACTTG GTGCGTTTGT CAAGCAAGGT AAGTGAACGA CCCGGTCATA CCTTCTTAAG TTCGCCCTTC CTCCCTTTAT TTCAGATTCA ATCTGACTTA CCTATTCTAC CCAAGCATCG ATATGAAAAA GCCTGAACTC ACCGCGACGT CTGTCGAGAA GTTTCTGATC GAAAAGTTCG ACAGCGTCTC CGACCTGATG CAGCTCTCGG AGGGCGAAGA ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT GTCCTGCGGG TAAATAGCTG CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC TTTGCATCGG CCGCGCTCCC GATTCCGGAA GTGCTTGACA TTGGGGAATT CAGCGAGAGC CTGACCTATT GCATCTCCCG CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC GAACTGCCCG CTGTTCTGCA GCCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT CTTAGCCAGA CGAGCGGGTT CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA TGGCGTGATT TCATATGCGC GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG GACGACACCG TCAGTGCGTC CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG GACTGCCCCG AAGTCCGGCA CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG GACAATGGCC GCATAACAGC GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA TACGAGGTCG CCAACATCTT CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG CGCTACTTCG AGCGGAGGCA TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG GTCTTGACCA ACTCTATCAG AGCTTGGTTG ACGGCAATTT CGATGATGCA GCTTGGGCGC AGGGTCGATG CGACGCAATC GTCCGATCCG GAGCCGGGAC TGTCGGGCGT ACACAAATCG CCCGCAGAAG CGCGGCCGTC TGGACCGATG GCTGTGTAGA AGTACTCGCC GATAGTGGAA ACCGACGCCC CAGCACTCGT CCGAGGGCAA AGGAATAGAG TAGATGCCGA CCGGATCGAT CCACTTAACG TTACTGAAAT CATCAAACAG CTTGACGAAT CTGGATATAA GATCGTTGGT GTCGATGTCA GCTCCGGAGT TGAGACAAAT GGTGTTCAGG ATCTCGATAA GATACGTTCA TTTGTCCAAG CAGCAAAGAG TGCCTTCTAG TGATTTAATA GCTCCATGTC AACAAGAATA AAACGCGTTT CGGGTTTACC TCTTCCAGAT ACAGCTCATC TGCAATGCAT TAATGCATTG GACCTCGCAA CCCTAGTACG CCCTTCAGGC TCCGGCGAAG CAGAAGAATA GCTTAGCAGA GTCTATTTTC ATTTTCGGGA GACGAGATCA AGCAGATCAA CGGTCGTCAA GAGACCTACG AGACTGAGGA ATCCGCTCTT GGCTCCACGC GACTATATAT TTGTCTCTAA TTGTACTTTG ACATGCTCCT CTTCTTTACT CTGATAGCTT GACTATGAAA ATTCCGTCAC CAGCCCCTGG GTTGCGGCCG CTCTAGAACT AGTGGATCCC CCGGGCTGCA GGAATTCATG CCAGTTGTTC CCAGTGATCT TCGTTTCGAA GATGGACACT CCCAATTTGT GCAAGTTATT CGGCCTACCT GGCTGTGGCC GAGGCGCGTT ATCATGACCG TCGCTGTTCA AAGATAAGGC GAGAAGTTTG CGGGCTGTCT TGACGATATG GCTTCGTTCA GACAGATATA GTTCCCGGAG TCGCAGGCGT CTATTCTTCT CCGAAACAAA CTCGGCTGCA CTGTTTCCAT CACCGGGTCT GGCGTTGAGG ATGTCAGCGA AACTCGGCCC GGCAAGTGAC ACCCGAAAAG TATCGACTCC GGCTGCCCGT TTCAAGCTAG TGGCTTCCTC ATCAGCGAGT CGGCCAAGGA GACGTGAAGC AGGACGGGTT TGCCATTCCA AGACCGTGAT CCGAAGCGCG TTGATTTCAT CAATCCCAGC CTTTTCGCTC AACCAAAGAG CATCGGCTTT GATTTCCTTC AGGTCATACG AGGCTTGTGC AATGGTCTCC GCATGGATCG CTGCTGTTCT CCTATCAAAC TCGGATTTTG TCTTAGGGGA TGGCGTAGGA AAGACGCTGC CGCGGTTCAG AAGCACCTCG ATGCTATCAG GATGTGACAA AAACGACTCG AAAACCCGGG ATTCATCGGT GATGCTTTCG GGATCGCAAG CGTAAAGAAA GACTCTCTTC CAAGACCTAG AAGTATAGCA AAATCAGCAG CAGACCATCA ATGTATAGCG AATGCGCCCA TACAAAAGCT GAACGTCCCC GGAGAAGCAC TTGTCCAGGG ACGGGAAATA GGCTTCCGGA ACGGGAGCCA TTGGCAGCAC AGCTATATCA TTCTAAGTAA ACAAATGTAA TGAGCAAGCG GACGGAGTGC TGAAACCTCC GTATGCCTGA AGCCGACGAA AGCGCGTTGG ATTAGAGGTC GACAGAAGAT GATATTGAAG GAGCACTTTT TGGGCTTGGC TGGAGCTAGT GGAGGTCAAC AATGAATGCC TATTTTGGTT TAGTCGTCCA GGCGGTGAGC ACAAAATTTG TGTCGTTTGA CAAGATGGTT CATTTAGGCA ACTGGTCAGA TCAGCCCCAC TTGTAGCAGT AGCGGCGGCG CTCGAAGTGT GACTCTTATT AGCAGACAGG AACGAGGACA TTATTATCAT CTGCTGCTTG GTGCACGATA ACTTGGTGCG TTTGTCAAGC AAGGTAAGTG AACGACCCGG TCATACCTTC TTAAGTTCGC CCTTCCTCCC TTTATTTCAG ATTCAATCTG ACTTACCTAT TCTACCCAAG CATCGATACC GTCGACCTCG AGGTACGTAC AAGCTTGCTG GAGGATACAG GTGAGCCTTT CTTCTTGCCT CTCTTTGTTT TTTTTTTGTT CTTTTTGCCG AATAGTGTAC CCACTGGAGA TTTGTTGGCC ATGCAAATAA ATGGAAGGGA CTGACAAGAT TGTGAAATTG TTCAAAACAC ACAGCCAGGG AACGGCAGAT CTTCGCATGC TAAGGCCTGT GGTACCTGGG CCCCGGATCC ACTTAACGTT ACTGAAATCA TCAAACAGCT TGACGAATCT GGATATAAGA TCGTTGGTGT CGATGTCAGC TCCGGAGTTG AGACAAATGG TGTTCAGGAT CTCGATAAGA TACGTTCATT TGTCCAAGCA GCAAAGAGTG CCTTCTAGTG ATTTAATAGC TCCATGTCAA CAAGAATAAA ACGCGTTTCG GGTTTACCTC TTCCAGATAC AGCTCATCTG CAATGCATTA ATGCATTGGA CCTCGCAACC CTAGTACGCC CTTCAGGCTC CGGCGAAGCA GAAGAATAGC TTAGCAGAGT CTATTTTCAT TTTCGGGAGA CGAGATCAAG CAGATCAACG GTCGTCAAGA GACCTACGAG ACTGAGGAAT CCGCTCTTGG CTCCACGCGA CTATATATTT GTCTCTAATT GTACTTTGAC ATGCTCCTCT TCTTTACTCT GATAGCTTGA CTATGAAAAT TCCGTCACCA GCCCCTGGGT TCGCAAAGAT AATTGCATGT TTCTTCCTTG AACTCTCAAG CCTACAGGAC ACACATTCAT CGTAGGTATA AACCTCGAAA TCATTCCTAC TAAGATGGTA TACAATAGTA ACCATGGTTG CCTAGTGAAT GCTCCGTAAC ACCCAATACG CCGGCCGAAA CTTTTTTACA ACTCTCCTAT GAGTCGTTTA CCCAGAATGC ACAGGTACAC TTGTTTAGAG GTAATCCTTC TTTCTAGAGG ATCCCAATTC GCCCTATAGT GAGTCGTATT ACGCGCGCTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGCTTACA ATTTAG
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