pBridge
$55.00
In stock
SKU
PL83542
DB00113;
100μg liquid plasmid(Instantly):$57
| name | pBridge | another name | apBridge |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | TRP1 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | PGBT9-R：ACAGGAAAGAGTTACTCAAG | |
| 3′ sequencing primer | PGBT9-F：GGCTTCAGTGGAGACTGATA | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AACGAAGCAT CTGTGCTTCA TTTTGTAGAA CAAAAATGCA ACGCGAGAGC GCTAATTTTT CAAACAAAGA ATCTGAGCTG CATTTTTACA GAACAGAAAT GCAACGCGAA AGCGCTATTT TACCAACGAA GAATCTGTGC TTCATTTTTG TAAAACAAAA ATGCAACGCG AGAGCGCTAA TTTTTCAAAC AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC TATTTTACCA ACAAAGAATC TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTC TAACAAAGCA TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG CAGTCTCTTG ATAACTTTTT GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG TGTCTATTTT CTCTTCCATA AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA GCGAAGCTGC GGGTGCATTT TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT GTGGATTGCG CATACTTTGT GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG AAAATTATGA ACGGTTTCTT CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA TTTTCGTATT GTTTTCGATT CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA GAGTAATACT AGAGATAAAC ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG AGCGAAAGGT GGATGGGTAG GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA TACTTTTGAG CAATGTTTGT GGAAGCGGTA TTCGCAATAT TTTAGTAGCT CGTTACAGTC CGGTGCGTTT TTGGTTTTTT GAAAGTGCGT CTTCAGAGCG CTTTTGGTTT TCAAAAGCGC TCTGAAGTTC CTATACTTTC TAGCTAGAGA ATAGGAACTT CGGAATAGGA ACTTCAAAGC GTTTCCGAAA ACGAGCGCTT CCGAAAATGC AACGCGAGCT GCGCACATAC AGCTCACTGT TCACGTCGCA CCTATATCTG CGTGTTGCCT GTATATATAT ATACATGAGA AGAACGGCAT AGTGCGTGTT TATGCTTAAA TGCGTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGGAAG ATCCGAGGCC TAGCTTTAAC GAACGCAGAA TTTTCGAGTT ATTAAACTTA AAATACGCTG AACCCGAACA TAGAAATATC GAATGGGAAA AAAAAACTGC ATAAAGGCAT TAAAAGAGGA GCGAATTTTT TTTTAATAAA AATCTTAATA ATCATTAAAA GATAAATAAT AGTCTATATA TACGTATATA AATAAAAAAT ATTCAAAAAA TAAAATAAAC TATTATTTTA GCGTAAAGGA TGGGGAAAGA GAAAAGAAAA AAATTGATCT ATCGATTTCA ATTCAATTCA ATTTATTTCT TTTCGGATAA GAAAGCAACA CCTGGCAATT CCTTACCTTC CAATAATTCC AAAGAAGCAC CACCACCAGT AGAGACATGG GAGATCAGCC CGAAGATCTT CGGGCTAATG CGGCCGCCAC CTTTCTCTTC TTCTTAGGAC TAGAAGGTCC TCCCAGGCTG GCATAGTCAG GCACGTCATA AGGATAGCTA GAAGCCATAT GGCCCATTGT ATGGATGGGG GTAATAGAAT TGTATCTATG TATCTGACGA CCCTGTATTA CACGAAGGAA ATAGTAGATG AAAAGACAAG AGAGCAAGAA AAAGGAAAAA CTTCCTTTCT AACAGACGCT TTACTTAACC TTATATATAT CTTATTTTTT TCATCGAGCG TGTTCAATTG GACAAGGTGC CACCTTTTCG ACACTTCGGT TATTATGTTA CACAGTTTTC ATGAGGATGG CGCCTTGACT AACTTAATTA GTCATTTGCC AACCACCACA GTTCCCCAAT ATCGACAGCT TCACGTGCCA TTTGCCATTT TGCGATCATG TGACCTCAAG AGAAAAAGCA AAAAATAATG AGAGCTAAGG GATTCGAACC CTTGCATCCG AAGATATCAG AGATTTTAGA GGTTAAATCC ACCTAAATCT GACGCCTTAA ACCACTCGGC CAAACTCCCA TTGTATGTTG GAAGAATTAG AAGCTAGGCC TCGGATCTTC CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTACCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACATGA TTACGAATTA ATTCGGTCGA AAAAAGAAAA GGAGAGGGCC AAGAGGGAGG GCATTGGTGA CTATTGAGCA CGTGAGTATA CGTGATTAAG CACACAAAGG CAGCTTGGAG TATGTCTGTT ATTAATTTCA CAGGTAGTTC TGGTCCATTG GTGAAAGTTT GCGGCTTGCA GAGCACAGAG GCCGCAGAAT GTGCTCTAGA TTCCGATGCT GACTTGCTGG GTATTATATG TGTGCCCAAT AGAAAGAGAA CAATTGACCC GGTTATTGCA AGGAAAATTT CAAGTCTTGT AAAAGCATAT AAAAATAGTT CAGGCACTCC GAAATACTTG GTTGGCGTGT TTCGTAATCA ACCTAAGGAG GATGTTTTGG CTCTGGTCAA TGATTACGGC ATTGATATCG TCCAACTGCA TGGAGATGAG TCGTGGCAAG AATACCAAGA GTTCCTCGGT TTGCCAGTTA TTAAAAGACT CGTATTTCCA AAAGACTGCA ACATACTACT CAGTGCAGCT TCACAGAAAC CTCATTCGTT TATTCCCTTG TTTGATTCAG AAGCAGGTGG GACAGGTGAA CTTTTGGATT GGAACTCGAT TTCTGACTGG GTTGGAAGGC AAGAGAGCCC CGAAAGCTTA CATTTTATGT TAGCTGGTGG ACTGACGCCA GAAAATGTTG GTGATGCGCT TAGATTAAAT GGCGTTATTG GTGTTGATGT AAGCGGAGGT GTGGAGACAA ATGGTGTAAA AGACTCTAAC AAAATAGCAA ATTTCGTCAA AAATGCTAAG AAATAGGTTA TTACTGAGTA GTATTTATTT AAGTATTGTT TGTGCACTTG CCGGCATGCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC ATGCTATACC TGAGAAAGCA ACCTGACCTA CAGGAAAGAG TTACTCAAGA ACAAGAATTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCCCGG AATTAGCTTG GCTGCAGGTC GACGGATCCC CGGGAATTCC GGCGATACAG TCAACTGTCT TTGACCTTTG TTACTACTCT CTTCCGATGA TGATGTCGCA CTTATTCTAT GCTGTCTCAA TGTTAGAGGC ATATCAGTCT CCACTGAAGC CAATCTATCT GTGACGGCAT CTTTATTCAC ATTATCTTGT ACAAATAATC CTGTTAACAA TGCTTTTATA TCCTGTAAAG AATCCATTTT CAAAATCATG TCAAGGTCTT CTCGAGGAAA AATCAGTAGA AATAGCTGTT CCAGTCTTTC TAGCCTTGAT TCCACTTCTG TCAGATGTGC CCTAGTCAGC GGAGACCTTT TGGTTTTGGG AGAGTAGCGA CACTCCCAGT TGTTCTTCAG ACACTTGGCG CACTTCGGTT TTTCTTTGGA GCACTTGAGC TTTTTAAGTC GGCAAATATC GCATGCTTGT TCGATAGAAG ACAGTAGCTT CATCTTTCAG GAGGCTTGCT TCAAGCTTGG AGTTGATTGT ATGCTTGGTA TAGCTTGAAA TATTGTGCAG AAAAAGAAAC AAGGAAGAAA GGGAACGAGA ACAATGACGA GGAAACAAAA GATTAATAAT TGCAGGTCTA TTTATACTTG ATAGCAAGAC AGCAAACTTT TTTTTATTTC AAATTCAAGT AACTGGAAGG AAGGCCGTAT ACCGTTGCTC ATTAGAGAGT AGTGTGCGTG AATGAAGGAA GGAAAAAGTT TCGTGTGCTT CGAGATACCC CTCATCAGCT CTGGAACAAC GACATCTGTT GGTGCTGTCT TTGTCGTTAA TTTTTTCCTT TAGTGTCTTC CATCATTTTT TTGTCATTGC GGATATGGTG AGACAACAAC GGGGGAGAGA GAAAAGAAAA AAAAAGAAAA GAAGTTGCAT GCAAGC
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