pGreen II-62-SK
$55.00
In stock
SKU
PL83515
DB00031;pGreenII-62-SK
100μg liquid plasmid(Instantly):$57
| name | pGreen II-62-SK | another name | apGreen II-62-SK |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | 35s | Replicon | pSa |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 35s： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTTTTATCCC CGGAAGCCTG TGGATAGAGG GTAGTTATCC ACGTGAAACC GCTAATGCCC CGCAAAGCCT TGATTCACGG GGCTTTCCGG CCCGCTCCAA AAACTATCCA CGTGAAATCG CTAATCAGGG TACGTGAAAT CGCTAATCGG AGTACGTGAA ATCGCTAATA AGGTCACGTG AAATCGCTAA TCAAAAAGGC ACGTGAGAAC GCTAATAGCC CTTTCAGATC AACAGCTTGC AAACACCCCT CGCTCCGGCA AGTAGTTACA GCAAGTAGTA TGTTCAATTA GCTTTTCAAT TATGAATATA TATATCAATT ATTGGTCGCC CTTGGCTTGT GGACAATGCG CTACGCGCAC CGGCTCCGCC CGTGGACAAC CGCAAGCGGT TGCCCACCGT CGAGCGCCAG CGCCTTTGCC CACAACCCGG CGGCCGGCCG CAACAGATCG TTTTATAAAT TTTTTTTTTT GAAAAAGAAA AAGCCCGAAA GGCGGCAACC TCTCGGGCTT CTGGATTTCC GATCCCCGGA ATTAGAGATC TTGGCAGGAT ATATTGTGGT GTAACGTTAT CAGCTTGGTA CGTACCCCCC TACTCCAAAA ATGTCAAAGA TACAGTCTCA GAAGACCAAA GGGCTATTGA GACTTTTCAA CAAAGGGTAA TTTCGGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTCATC GAAAGGACAG TAGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCTATCATTC AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAC ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA GTCATTTCAT TTGGAGAGGA CAGCCCAAGC TGAGCTCCAC CGCGGTGGCG GCCGCTCTAG AACTAGTGGA TCCCCCGGGC TGCAGGAATT CGATATCAAG CTTATCGATA CCGTCGACCT CGAGGGGGGG CCCGGTACCA ATTCGGTACG CTGAAATCAC CAGTCTCTCT CTACAAATCT ATCTCTCTCT ATTTTCTCCA TAAATAATGT GTGAGTAGTT TCCCGATAAG GGAAATTAGG GTTCTTATAG GGTTTCGCTC ATGTGTTGAG CATATAAGAA ACCCTTAGTA TGTATTTGTA TTTGTAAAAT ACTTCTATCA ATAAAATTTC TAATTCCTAA AACCAAAATC CAGTACTAAA ATCCAGATCC ACTAGCCTTG ACAGGATATA TTGGCGGGTA AACTAAGTCG CTGTATGTGT TTGTTTGAGA TCTCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AGAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGTGTAACA TTGGTCTAGT GATTAGAAAA ACTCATCGAG CATCAAATGA AACTGCAATT TATTCATATC AGGATTATCA ATACCATATT TTTGAAAAAG CCGTTTCTGT AATGAAGGAG AAAACTCACC GAGGCAGTTC CATAGGATGG CAAGATCCTG GTATCGGTCT GCGATTCCGA CTCGTCCAAC ATCAATACAA CCTATTAATT TCCCCTCGTC AAAAATAAGG TTATCAAGTG AGAAATCACC ATGAGTGACG ACTGAATCCG GTGAGAATGG CAAAAGTTTA TGCATTTCTT TCCAGACTTG TTCAACAGGC CAGCCATTAC GCTCGTCATC AAAATCACTC GCATCAACCA AACCGTTATT CATTCGTGAT TGCGCCTGAG CGAGACGAAA TACGCGATCG CTGTTAAAAG GACAATTACA AACAGGAATC GAATGCAACC GGCGCAGGAA CACTGCCAGC GCATCAACAA TATTTTCACC TGAATCAGGA TATTCTTCTA ATACCTGGAA TGCTGTTTTC CCTGGGATCG CAGTGGTGAG TAACCATGCA TCATCAGGAG TACGGATAAA ATGCTTGATG GTCGGAAGAG GCATAAATTC CGTCAGCCAG TTTAGTCTGA CCATCTCATC TGTAACAACA TTGGCAACGC TACCTTTGCC ATGTTTCAGA AACAACTCTG GCGCATCGGG CTTCCCATAC AATCGGTAGA TTGTCGCACC TGATTGCCCG ACATTATCGC GAGCCCATTT ATACCCATAT AAATCAGCAT CCATGTTGGA ATTTAATCGC GGCCTTGAGC AAGACGTTTC CCGTTGAATA TGGCTCATAA CACCCCTTGT ATTACTGTTT ATGTAAGCAG ACAGTTTTAT TGTTCATGAT GATATATTTT TATCTTGTGC AATGTAACAT CAGAGATTTT GAGACACAAC GTGGCTTTGT TGAATAAATC GAACTTTTGC TGAGTTGAAG GATCAGATCA CGCATCTTCC CGACAACGCA GACCGTTCCG TGGCAAAGCA AAAGTTCAAA ATCACCAACT GGTCCACCTA CAACAAAGCT CTCATCAACC GTGGCTCCCT CACTTTCTGG CTGGATGATG GGGCGATTCA GGCGATCCCC ATCCAACAGC CCGCCGTCGA GCGGGCT
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