pAN7-1
$84.00
In stock
SKU
PL83514
DB00082;Z32698.1
100μg liquid plasmid(Instantly):$101
| name | pAN7-1 | another name | apAN7-1 |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | gpdA | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTCCCTT GTATCTCTAC ACACAGGCTC AAATCAATAA GAAGAACGGT TCGTCTTTTT CGTTTATATC TTGCATCGTC CCAAAGCTAT TGGCGGGATA TTCTGTTTGC AGTTGGCTGA CTTGAAGTAA TCTCTGCAGA TCTTTCGACA CTGAAATACG TCGAGCCTGC TCCGCTTGGA AGCGGCGAGG AGCCTCGTCC TGTCACAACT ACCAACATGG AGTACGATAA GGGCCAGTTC CGCCAGCTCA TTAAGAGCCA GTTCATGGGC GTTGGCATGA TGGCCGTCAT GCATCTGTAC TTCAAGTACA CCAACGCTCT TCTGATCCAG TCGATCATCC GCTGAAGGCG CTTTCGAATC TGGTTAAGAT CCACGTCTTC GGGAAGCCAG CGACTGGTGA CCTCCAGCGT CCCTTTAAGG CTGCCAACAG CTTTCTCAGC CAGGGCCAGC CCAAGACCGA CAAGGCCTCC CTCCAGAACG CCGAGAAGAA CTGGAGGGGT GGTGTCAAGG AGGAGTAAGC TCCTTATTGA AGTCGGAGGA CGGAGCGGTG TCAAGAGGAT ATTCTTCGAC TCTGTATTAT AGATAAGATG ATGAGGAATT GGAGGTAGCA TAGCTTCATT TGGATTTGCT TTCCAGGCTG AGACTCTAGC TTGGAGCATA GAGGGTCCTT TGGCTTTCAA TATTCTCAAG TATCTCGAGT TTGAACTTAT TCCCTGTGAA CCTTTTATTC ACCAATGAGC ATTGGAATGA ACATGAATCT GAGGACTGCA ATCGCCATGA GGTTTTCGAA ATACATCCGG ATGTCGAAGG CTTGGGGCAC CTGCGTTGGT TGAATTTAGA ACGTGGCACT ATTGATCATC CGATAGCTCT GCAAAGGGCG TTGCACAATG CAAGTCAAAC GTTGCTAGCA GTTCCAGGTG GAATGTTATG ATGAGCATTG TATTAAATCA GGAGATATAG CATGATCTCT AGTTAGCTCA CCACAAAAGT CAGACGGCGT AACCAAAAGT CACACAACAC AAGCTGTAAG GATTTCGGCA CGGCTACGGA AGACGGAGAA GCCACCTTCA GTGGACTCGA GTACCATTTA ATTCTATTTG TGTTTGATCG AGACCTAATA CAGCCCCTAC AACGACCATC AAAGTCGTAT AGCTACCAGT GAGGAAGTGG ACTCAAATCG ACTTCAGCAA CATCTCCTGG ATAAACTTTA AGCCTAAACT ATACAGAATA AGATAGGTGG AGAGCTTATA CCGAGCTCCC AAATCTGTCC AGATCATGGT TGACCGGTGC CTGGATCTTC CTATAGAATC ATCCTTATTC GTTGACCTAG CTGATTCTGG AGTGACCCAG AGGGTCATGA CTTGAGCCTA AAATCCGCCG CCTCCACCAT TTGTAGAAAA ATGTGACGAA CTCGTGAGCT CTGTACAGTG ACCGGTGACT CTTTCTGGCA TGCGGAGAGA CGGACGGACG CAGAGAGAAG GGCTGAGTAA TAAGCCACTG GCCAGACAGC TCTGGCGGCT CTGAGGTGCA GTGGATGATT ATTAATCCGG GACCGGCCGC CCCTCCGCCC CGAAGTGGAA AGGCTGGTGT GCCCCTCGTT GACCAAGAAT CTATTGCATC ATCGGAGAAT ATGGAGCTTC ATCGAATCAC CGGCAGTAAG CGAAGGAGAA TGTGAAGCCA GGGGTGTATA GCCGTCGGCG AAATAGCATG CCATTAACCT AGGTACAGAA GTCCAATTGC TTCCGATCTG GTAAAAGATT CACGAGATAG TACCTTCTCC GAAGTAGGTA GAGCGAGTAC CCGGCGCGTA AGCTCCCTAA TTGGCCCATC CGGCATCTGT AGGGCGTCCA AATATCGTGC CTCTCCTGCT TTGCCCGGTG TATGAAACCG GAAAGGCCGC TCAGGAGCTG GCCAGCGGCG CAGACCGGGA ACACAAGCTG GCAGTCGACC CATCCGGTGC TCTGCACTCG ACCTGCTGAG GTCCCTCAGT CCCTGGTAGG CAGCTTTGCC CCGTCTGTCC GCCCGGTGTG TCGGCGGGGT TGACAAGGTC GTTGCGTCAG TCCAACATTT GTTGCCATAT TTTCCTGCTC TCCCCACCAG CTGCTCTTTT CTTTTCTCTT TCTTTTCCCA TCTTCAGTAT ATTCATCTTC CCATCCAAGA ACCTTTATTT CCCCTAAGTA AGTACTTTGC TACATCCATA CTCCATCCTT CCCATCCCTT ATTCCTTTGA ACCTTTCAGT TCGAGCTTTC CCACTTCATC GCAGCTTGAC TAACAGCTAC CCCGCTTGAG CAGACATCAC CATGCCTGAA CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG AGCCTGACCT ATTGCATCTC CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG ATGGACGACA CCGTCAGTGC GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC GAGGACTGCC CCGAAGTCCG GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG ACGGACAATG GCCGCATAAC AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC CAATACGAGG TCGCCAACAT CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG ACGCGCTACT TCGAGCGGAG GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA CCAACTCTAT CAGAGCTTGG TTGACGGCAA TTTCGATGAT GCAGCTTGGG CGCAGGGTCG ATGCGACGCA ATCGTCCGAT CCGGAGCCGG GACTGTCGGG CGTACACAAA TCGCCCGCAG AAGCGCGGCC GTCTGGACCG ATGGCTGTGT AGAAGTACTC GCCGATAGTG GAAACCGACG CCCCAGCACT CGTCCGAGGG CAAAGGAATA GAGTAGATGC CGACCGCGGG ATCCACTTAA CGTTACTGAA ATCATCAAAC AGCTTGACGA ATCTGGATAT AAGATCGTTG GTGTCGATGT CAGCTCCGGA GTTGAGACAA ATGGTGTTCA GGATCTCGAT AAGATACGTT CATTTGTCCA AGCAGCAAAG AGTGCCTTCT AGTGATTTAA TAGCTCCATG TCAACAAGAA TAAAACGCGT TTTCGGGTTT ACCTCTTCCA GATACAGCTC ATCTGCAATG CATTAATGCA TTGACTGCAA CCTAGTAACG CCTTnCAGGC TCCGGCGAAG AGAAGAATAG CTTAGCAGAG CTATTTTCAT TTTCGGGAGA CGAGATCAAG CAGATCAACG GTCGTCAAGA GACCTACGAG ACTGAGGAAT CCGCTCTTGG CTCCACGCGA CTATATATTT GTCTCTAATT GTACTTTGAC ATGCTCCTCT TCTTTACTCT GATAGCTTGA CTATGAAAAT TCCGTCACCA GCnCCTGGGT TCGCAAAGAT AATTGCATGT TTCTTCCTTG AACTCTCAAG CCTACAGGAC ACACATTCAT CGTAGGTATA AACCTCGAAA TCAnTTCCTA CTAAGATGGT ATACAATAGT AACCATGCAT GGTTGCCTAG TGAATGCTCC GTAACACCCA ATACGCCGGC CGAAACTTTT TTACAACTCT CCTATGAGTC GTTTACCCAG AATGCACAGG TACACTTGTT TAGAGGTAAT CCTTCTTTCT AGAAGTCCTC GTGTACTGTG TAAGCGCCCA CTCCACATCT CCACTCGACC TGCAGGCATG CAAGCTTGGC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTAC
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