pXMJ19
$55.00
In stock
SKU
PL83478
DB00087;
100μg liquid plasmid(Instantly):$57
| name | pXMJ19 | another name | apXMJ19 |
| Insert Size (bp) | Plasmid host | Corynebacterium glutamate | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Fluorescent labeling | ||
| Promoter | Tac | Replicon | pBL1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | Tac-P：gacaattaatcatcggctcg | |
| 3′ sequencing primer | AmpNR：GAATAAGGGCGACACGGAAATG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTAAGCTT GCATGCCTGC AGGTCGACTC TAGAGGATCC CCGGGTACCG AGCTCGAATT CAGCTTGGCT GTTTTGGCGG ATGAGAGAAG ATTTTCAGCC TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA ACAGAATTTG CCTGGCGGCA GTAGCGCGGT GGTCCCACCT GACCCCATGC CGAACTCAGA AGTGAAACGC CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCTACAAACT CTTTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTG GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA GCCATTCGGG GTCGTTCACT GGTTCCCCTT TCTGATTTCT GGCATAGAAG AACCCCCGTG AACTGTGTGG TTCCGGGGGT TGCTGATTTT TGCGAGACTT CTCGCGCAAT TCCCTAGCTT AGGTGAAAAC ACCATGAAAC ACTAGGGAAA CACCCATGAA ACACCCATTA GGGCAGTAGG GCGGCTTCTT CGTCTAGGGC TTGCATTTGG GCGGTGATCT GGTCTTTAGC GTGTGAAAGT GTGTCGTAGG TGGCGTGCTC AATGCACTCG AACGTCACGT CATTTACCGG GTCACGGTGG GCAAAGAGAA CTAGTGGGTT AGACATTGTT TTCCTCGTTG TCGGTGGTGG TGAGCTTTTC TAGCCGCTCG GTAAACGCGG CGATCATGAA CTCTTGGAGG TTTTCACCGT TCTGCATGCC TGCGCGCTTC ATGTCCTCAC GTAGTGCCAA AGGAACGCGT GCGGTGACCA CGACGGGCTT AGCCTTTGCC TGCGCTTCTA GTGCTTCGAT GGTGGCTTGT GCCTGCGCTT GCTGCGCCTG TAGTGCCTGT TGAGCTTCTT GTAGTTGCTG TTCTAGCTGT GCCTTGGTTG CCATGCTTTA AGACTCTAGT AGCTTTCCTG CGATATGTCA TGCGCATGCG TAGCAAACAT TGTCCTGCAA CTCATTCATT ATGTGCAGTG CTCCTGTTAC TAGTCGTACA TACTCATATT TACCTAGTCT GCATGCAGTG CATGCACATG CAGTCATGTC GTGCTAATGT GTAAAACATG TACATGCAGA TTGCTGGGGG TGCAGGGGGC GGAGCCACCC TGTCCATGCG GGGTGTGGGG CTTGCCCCGC CGGTACAGAC AGTGAGCACC GGGGCACCTA GTCGCGGATA CCCCCCCTAG GTATCGGACA CGTAACCCTC CCATGTCGAT GCAAATCTTT AACATTGAGT ACGGGTAAGC TGGCACGCAT AGCCAAGCTA GGCGGCCACC AAACACCACT AAAAATTAAT AGTCCCTAGA CAAGACAAAC CCCCGTGCGA GCTACCAACT CATATGCACG GGGGCCACAT AACCCGAAGG GGTTTCAATT GACAACCATA GCACTAGCTA AGACAACGGG CACAACACCC GCACAAACTC GCACTGCGCA ACCCCGCACA ACATCGGGTC TAGGTAACAC TGAGTAACAC TGAAATAGAA GTGAACACCT CTAAGGAACC GCAGGTCAAT GAGGGTTCTA AGGTCACTCG CGCTAGGGCG TGGCGTAGGC AAAACGTCAT GTACAAGATC ACCAATAGTA AGGCTCTGGC GGGGTGCCAT AGGTGGCGCA GGGACGAAGC TGTTGCGGTG TCCTGGTCGT CTAACGGTGC TTCGCAGTTT GAGGGTCTGC AAAACTCTCA CTCTCGCTGG GGGTCACCTC TGGCTGAATT GGAAGTCATG GGCGAACGCC GCATTGAGCT GGCTATTGCT ACTAAGAATC ACTTGGCGGC GGGTGGCGCG CTCATGATGT TTGTGGGCAC TGTTCGACAC AACCGCTCAC AGTCATTTGC GCAGGTTGAA GCGGGTATTA AGACTGCGTA CTCTTCGATG GTGAAAACAT CTCAGTGGAA GAAAGAACGT GCACGGTACG GGGTGGAGCA CACCTATAGT GACTATGAGG TCACAGACTC TTGGGCGAAC GGTTGGCACT TGCACCGCAA CATGCTGTTG TTCTTGGATC GTCCACTGTC TGACGATGAA CTCAAGGCGT TTGAGGATTC CATGTTTTCC CGCTGGTCTG CTGGTGTGGT TAAGGCCGGT ATGGACGCGC CACTGCGTGA GCACGGGGTC AAACTTGATC AGGTGTCTAC CTGGGGTGGA GACGCTGCGA AAATGGCAAC CTACCTCGCT AAGGGCATGT CTCAGGAACT GACTGGCTCC GCTACTAAAA CCGCGTCTAA GGGGTCGTAC ACGCCGTTTC AGATGTTGGA TATGTTGGCC GATCAAAGCG ACGCCGGCGA GGATATGGAC GCTGTTTTGG TGGCTCGGTG GCGTGAGTAT GAGGTTGGTT CTAAAAACCT GCGTTCGTCC TGGTCACGTG GGGCTAAGCG TGCTTTGGGC ATTGATTACA TAGACGCTGA TGTACGTCGT GAAATGGAAG AAGAACTGTA CAAGCTCGCC GGTCTGGAAG CACCGGAACG GGTCGAATCA ACCCGCGTTG CTGTTGCTTT GGTGAAGCCC GATGATTGGA AACTGATTCA GTCTGATTTC GCGGTTAGGC AGTACGTTCT CGATTGCGTG GATAAGGCTA AGGACGTGGC CGCTGCGCAA CGTGTCGCTA ATGAGGTGCT GGCAAGTCTG GGTGTGGATT CCACCCCGTG CATGATCGTT ATGGATGATG TGGACTTGGA CGCGGTTCTG CCTACTCATG GGGACGCTAC TAAGCGTGAT CTGAATGCGG CGGTGTTCGC GGGTAATGAG CAGACTATTC TTCGCACCCA CTAAAAGCGG CATAAACCCC GTTCGATATT TTGTGCGATG AATTTATGGT CAATGTCGCG GGGGCAAACT ATGATGGGTC TTGTTGTTGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAAGGGCACC AATAACTGCC TTAAAAAAAT TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA TGGAAGCCAT CACAGACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGTC GAAGCTCGGC GGATTTGTCC TACTCAAGCT GATCCGACAA AATCCACACA TTATCCCAGG TGTCCGGATC GGTCAAATAC GCTGCCAGCT CATAGACCGT ATCCAAAGCA TCCGGGGCTG ATCCCCGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCACCATTCG ATGGTGTCAA CGTAAATGCC GCTTCGCCTT CGCGCGCGAA TTGCAAGCTG ATCCGGGCTT ATCGACTGCA CGGTGCACCA ATGCTTCTGG CGTCAGGCAG CCATCGGAAG CTGTGGTATG GCTGTGCAGG TCGTAAATCA CTGCATAATT CGTGTCGCTC AAGGCGCACT CCCGTTCTGG ATAATGTTTT TTGCGCCGAC ATCATAACGG TTCTGGCAAA TATTCTGAAA TGAGCTGTTG ACAATTAATC ATCGGCTCGT ATAATGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGAAT T
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