pCDFDuet-1
$99.00
In stock
SKU
PL83341
pCDFDuet1
100μg liquid plasmid(Instantly):$120
| name | pCDFDuet-1 | another name | apCDFDuet-1 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Multiple frames unloaded | Prokaryotic resistance | Str |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | CDF |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ACYCDuetUP1：GGATCTCGACGCTCTCCCT | |
| 3′ sequencing primer | T7ter：TGCTAGTTATTGCTCAGCGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGGAATTGT GAGCGGATAA CAATTCCCCT GTAGAAATAA TTTTGTTTAA CTTTAATAAG GAGATATACC ATGGGCAGCA GCCATCACCA TCATCACCAC AGCCAGGATC CGAATTCGAG CTCGGCGCGC CTGCAGGTCG ACAAGCTTGC GGCCGCATAA TGCTTAAGTC GAACAGAAAG TAATCGTATT GTACACGGCC GCATAATCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCA TCTTAGTATA TTAGTTAAGT ATAAGAAGGA GATATACATA TGGCAGATCT CAATTGGATA TCGGCCGGCC ACGCGATCGC TGACGTCGGT ACCCTCGAGT CTGGTAAAGA AACCGCTGCT GCGAAATTTG AACGCCAGCA CATGGACTCG TCTACTAGCG CAGCTTAATT AACCTAGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAACCTCA GGCATTTGAG AAGCACACGG TCACACTGCT TCCGGTAGTC AATAAACCGG TAAACCAGCA ATAGACATAA GCGGCTATTT AACGACCCTG CCCTGAACCG ACGACCGGGT CATCGTGGCC GGATCTTGCG GCCCCTCGGC TTGAACGAAT TGTTAGACAT TATTTGCCGA CTACCTTGGT GATCTCGCCT TTCACGTAGT GGACAAATTC TTCCAACTGA TCTGCGCGCG AGGCCAAGCG ATCTTCTTCT TGTCCAAGAT AAGCCTGTCT AGCTTCAAGT ATGACGGGCT GATACTGGGC CGGCAGGCGC TCCATTGCCC AGTCGGCAGC GACATCCTTC GGCGCGATTT TGCCGGTTAC TGCGCTGTAC CAAATGCGGG ACAACGTAAG CACTACATTT CGCTCATCGC CAGCCCAGTC GGGCGGCGAG TTCCATAGCG TTAAGGTTTC ATTTAGCGCC TCAAATAGAT CCTGTTCAGG AACCGGATCA AAGAGTTCCT CCGCCGCTGG ACCTACCAAG GCAACGCTAT GTTCTCTTGC TTTTGTCAGC AAGATAGCCA GATCAATGTC GATCGTGGCT GGCTCGAAGA TACCTGCAAG AATGTCATTG CGCTGCCATT CTCCAAATTG CAGTTCGCGC TTAGCTGGAT AACGCCACGG AATGATGTCG TCGTGCACAA CAATGGTGAC TTCTACAGCG CGGAGAATCT CGCTCTCTCC AGGGGAAGCC GAAGTTTCCA AAAGGTCGTT GATCAAAGCT CGCCGCGTTG TTTCATCAAG CCTTACGGTC ACCGTAACCA GCAAATCAAT ATCACTGTGT GGCTTCAGGC CGCCATCCAC TGCGGAGCCG TACAAATGTA CGGCCAGCAA CGTCGGTTCG AGATGGCGCT CGATGACGCC AACTACCTCT GATAGTTGAG TCGATACTTC GGCGATCACC GCTTCCCTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGCTAG CTCACTCGGT CGCTACGCTC CGGGCGTGAG ACTGCGGCGG GCGCTGCGGA CACATACAAA GTTACCCACA GATTCCGTGG ATAAGCAGGG GACTAACATG TGAGGCAAAA CAGCAGGGCC GCGCCGGTGG CGTTTTTCCA TAGGCTCCGC CCTCCTGCCA GAGTTCACAT AAACAGACGC TTTTCCGGTG CATCTGTGGG AGCCGTGAGG CTCAACCATG AATCTGACAG TACGGGCGAA ACCCGACAGG ACTTAAAGAT CCCCACCGTT TCCGGCGGGT CGCTCCCTCT TGCGCTCTCC TGTTCCGACC CTGCCGTTTA CCGGATACCT GTTCCGCCTT TCTCCCTTAC GGGAAGTGTG GCGCTTTCTC ATAGCTCACA CACTGGTATC TCGGCTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTAA GCAAGAACTC CCCGTTCAGC CCGACTGCTG CGCCTTATCC GGTAACTGTT CACTTGAGTC CAACCCGGAA AAGCACGGTA AAACGCCACT GGCAGCAGCC ATTGGTAACT GGGAGTTCGC AGAGGATTTG TTTAGCTAAA CACGCGGTTG CTCTTGAAGT GTGCGCCAAA GTCCGGCTAC ACTGGAAGGA CAGATTTGGT TGCTGTGCTC TGCGAAAGCC AGTTACCACG GTTAAGCAGT TCCCCAACTG ACTTAACCTT CGATCAAACC ACCTCCCCAG GTGGTTTTTT CGTTTACAGG GCAAAAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACTGA ACCGCTCTAG ATTTCAGTGC AATTTATCTC TTCAAATGTA GCACCTGAAG TCAGCCCCAT ACGATATAAG TTGTAATTCT CATGTTAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA CTACCGAGAT GTCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA GACTGGAGGT GGCAACGCCA ATCAGCAACG ACTGTTTGCC CGCCAGTTGT TGTGCCACGC GGTTGGGAAT GTAATTCAGC TCCGCCATCG CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG AAACGTGGCT GGCCTGGTTC ACCACGCGGG AAACGGTCTG ATAAGAGACA CCGGCATACT CTGCGACATC GTATAACGTT ACTGGTTTCA CATTCACCAC CCTGAATTGA CTCTCTTCCG GGCGCTATCA TGCCATACCG CGAAAGGTTT TGCGCCATTC GATGGTGTCC GGGATCTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAATTAATAC GACTCACTAT A
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
