pLpp-OmpA
$99.00
In stock
SKU
PL83325
pLLP-OmpA
100μg liquid plasmid(Instantly):$120
| name | pLpp-OmpA | another name | apLpp-OmpA |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13R：CAGGAAACAGCTATGACC | |
| 3′ sequencing primer | OmpA：AGAACAGCAGAACGTTCAGAC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGATAACCAG AAGCAATAAA AAATCAAATC GGATTTCACT ATATAATCTC ACTTTATCTA AGATGAATCC GATGGAAGCA TCCTGTTTTC TCTCAATTTT TTTATCTAAA ACCCAGCGTT CGATGCTTCT TTGAGCGAAC GATCAAAAAT AAGTGCCTTC CCATCAAAAA AATATTCTCA ACATAAAAAA CTTTGTGTAA TACTTGTAAC GCTACATGGA GATTAACTCA ATCTAGCTAG AGAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGAATTGTGA GCGGATAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGGATTC ACTGGAACTC TAGATAACGA GGCGCAAAAA ATGAAAAAGA CAGCTATCGC GATTGCAGTG GCACTGGCAG GTTTCGCTAC CGTCGCTCAG GCTGGATCCG CAGAATTCAG CGCTAGCCAC CATCATCATC ATCATTAAGC TTAGATCCGG CTGAGCAACG ACGTGAACGC AATGCGTTCC GACGTTCAGG CTGCTAAAGA TGACGCAGCT CGTGCTAACC AGCGTCTGGA CAACATGGCT ACTAAATACC GCAAGTAATA GTACCTGTGA AGTGAAAAAT GGCGCACATT GTGCGACATT TTTTTTGTCT GCCGTTTACC GCTACTGCGT CACGCGTAAC ATATTCCCTT GCTCTGGTTC ACCATTCTGC GCTGACTCTA CTGAAGGCGC ATTGCTGGCT GCGGGAGTTG CTCCACTGCT CACCGAAACC GGATACCCTG CCCGACGATA CAACGCTTTA TCGACTAACT TCTGATCTAC AGCCTTATTG TCTTTAAATT GCGTAAAGCC TGCTGGCAGT GTGTATGGCA TTGTCTGAAC GTTCTGCTGT TCTTCTGCCG ATAGTGGTCG ATGTACTTCA ACATAACGCA TCCCGTTAGG CTCCACGGAA TATTTCACCG GTTCGTTGAT CACTTTCACC GGCGTTCCCG TCCGCACGCT GGAGAACAAG GCTTTAATAT CCGGTGCATT CATGCGAATA CACCCTGAAC TGACGCGCAA ACCGACGCTG TCCGGCGCAC TGGTACCATG AATGAGGTAT TCGCCATTAC CATGCGCGAG GCGCAGTGCG TAACGTCCTA GCGGGTTATT TGGTCCGGCA GGAACGACTG GCGGTAATTT AATGCCACGC TCCAGCGAAC GCTGACGAAT GCCTGCCGTA GGCGTCCAGG TTGGGTTAGG GATTTTCTGC CCAACACGCG TTTCCATCAC CGGCGTTTCC AGCCCCTGCA ATCCAATACC TATTGGATAA ACCTGCACAA TATTTTCTCC CGGCGGATAA TAATAAAGGC GCAGCTCTGC AAGGTTGGTC GACGGTCGAC CGGTCTCACT TCAACATAAC GCATCCCGTT AGGCTCCACG GAATATTTCA CCGGTTCGTT GATCACTTTC ACCGGCGTTC CCGTCCGCAC GCTGGAGAAC AAGGCTTTAA TATCCGGTGC ATTCATGCGA ATACACCCTG AACTGACGCG CAAACCGACG CTGTCCGGCG CACTGGTACC ATGAATGAGG TATTCGCCAT TACCATGCGC GAGGCGCAGT GCGTAACGTC CTAGCGGGTT ATTTGGTCCG GCAGGAACGA CTGGCGGTAA TTTAATGCCA CGCTCCAGCG AACGCTGACG AATGCCTGCC GTAGGCGTCC AGGTTGGGTT AGGGATTTTC TGCCCAACAC GCGTTTCCAT CACCGGCGTT TCCAGCCCCT GCAATCCAAT ACCTATTGGA TAAACCTGCA CAATATTTTC TCCCGGCGGA TAATAATAAA GGCGCAGCTC TGCAAGGTTG ACACCATCGA ATGGCGCAAA ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC AATTCAGGGT GGTGAATGTG AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG CTGATCATTA ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTC TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT GGCTGGCTGG CATAAATATC TCACTCGCAA TCAAATTCAG CCGATAGCGG AACGGGAAGG CGACTGGAGT GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG AGGGCATCGT TCCCACTGCG ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC GCGCCATTAC CGAGTCCGGG CTGCGCGTTG GTGCGGATAT CTCGGTAGTG GGATACGACG ATACCGAAGA CAGCTCATGT TATATCCCGC CGTTAACCAC CATCAAACAG GATTTTCGCC TGCTGGGGCA AACCAGCGTG GACCGCTTGC TGCAACTCTC TCAGGGCCAG GCGGTGAAGG GCAATCAGCT GTTGCCCGTC TCACTGGTGA AAAGAAAAAC CACCCTGGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTAACGATA ATCCCTGACG CGGTGCATCA GGTAATAACA GTTGTGAAGG AATAGTTATC GTCGTACCAG GTTTTGGCAC CGGGGCGATA GTGTTATTGG CTTCAAGGAT CAACATTGCC GCAGTATCAA AACGTCGGGC AATAGCCTGA AGGTTTTTAT CCCCTTCTTG CACCGTATAC GTTTGATTTT GCCCAACCAG TCGGCTTCCG GTTGGTGGTA GCGGATAATC AACCGCCCAG GCAGCCTGGA TGGCGCTAAA AGCGCCGATA AGCGTGAGTG TAAGCAAAGA CGCGCGTTTC ATTGTAAACC TCCTGTATTT GCCGGAGACT CACGCTGAAA CGTCGGATGG CGCTTATGTT CACCTGAAAC CAAAACACTC CTGTGCAGGT CAGTGTAAAC ATTGACCATC CGGCAATGTG AGCCAACCGG ATGAAAGCTG TCCTTTTAGT TTAGCTAAGT GCAGCGGCTT TGGCGCGAAT TGCGCGAATC ATCGCTTCCA GACCTTGTGA ACGAGATGGG GTGAGATGTT GGGTGAGCGC CATTTTTTCA AACCACGGAC GCACATCGAA ATTGACAATA TCCTGCGGCG TCATCTGATC GTAGAGAATA AAGACGACCG CAATAAGCCC TTTCACAATC GCCGCATCGC TGTCGCCCTG TAATTCAATA ATTCCCTGGG CATTCTGGCG CATGACAATC CACACCTGAC TCTGACAGCC CTGAATGCTA TTTTGTGGAC TTCTGTCTTC GTCGCGTAAT TCTGGCAGAC GCTGGGGGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC ACTTATGACT GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTGGGT CATTTTCGGC GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT CGCTTGCGGT ATTCGGAATC TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA CCAAACGTTT CGGCGAGAAG CAGGCCATTA TCGCCGGCAT GGCGGCCGAC GCGCTGGGCT ACGTCTTGCT GGCGTTCGCG ACGCGAGGCT GGATGGCCTT CCCCATTATG ATTCTTCTCG CTTCCGGCGG CATCGGGATG CCCGCGTTGC AGGCCATGCT GTCCAGGCAG GTAGATGACG ACCATCAGGG ACAGCTTCAA GGATCGCTCG CGGCTCTTAC CAGCCTAACT TCGATCACTG GACCGCTGAT CGTCACGGCG ATTTATGCCG CCTCGGCGAG CACATGGAAC GGGTTGGCAT GGATTGTAGG CGCCGCCCTA TACCTTGTCT GCCTCCCCGC GTTGCGTCGC GGTGCATGGA GCCGGGCCAC CTCGACCTGA ATGGAAGCCG GCGGCACCTC GCTAACGGAT TCACCACTCC AAGAATTGGA GCCAATCAAT TCTTGCGGAG AACTGTGAAT GCGCAAACCA ACCCTTGGCA GAACATATCC ATCGCGTCCG CCATCTCCAG CAGCCGCACG CGGCGCATCT CGGGCAGCGT TGGGTCCTGG CCACGGGTGC GCATGATCGT GCTCCTGTCG TTGAGGACCC GGCTAGGCTG GCGGGGTTGC CTTACTGGTT AGCAGAATGA ATCACCGATA CGCGAGCGAA CGTGAAGCGA CTGCTGCTGC AAAACGTCTG CGACCTGAGC AACAACATGA ATGGTCTTCG GTTTCCGTGT TTCGTAAAGT CTGGAAACGC GGAAGTCAGC GCCCTGCACC ATTATGTTCC GGATCTGCAT CGCAGGATGC TGCTGGCTAC CCTGTGGAAC ACCTACATCT GTATTAACGA AGCGCTGGCA TTGACCCTGA GTGATTTTTC TCTGGTCCCG CCGCATCCAT ACCGCCAGTT GTTTACCCTC ACAACGTTCC AGTAACCGGG CATGTTCATC ATCAGTAACC CGTATCGTGA GCATCCTCTC TCGTTTCATC GGTATCATTA CCCCCATGAA CAGAAATCCC CCTTACACGG AGGCATCAGT GACCAAACAG GAAAAAACCG CCCTTAACAT GGCCCGCTTT ATCAGAAGCC AGACATTAAC GCTTCTGGAG AAACTCAACG AGCTGGACGC GGATGAACAG GCAGACATCT GTGAATCGCT TCACGACCAC GCTGATGAGC TTTACCGCAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTT
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