pIRESPuro3-ITGB3
$128.00
In stock
SKU
PL83317
NM_000212.2;GT;CD61;GP3A;BDPLT2;GPIIIa;BDPLT16
100μg liquid plasmid(Instantly):$149
| name | pIRESPuro3-ITGB3 | another name | apIRESPuro3-ITGB3 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CGATATCTGC GGCCTAGCTA GCGCCACCAT GCGAGCGCGG CCGCGGCCCC GGCCGCTCTG GGCGACTGTG CTGGCGCTGG GGGCGCTGGC GGGCGTTGGC GTAGGAGGGC CCAACATCTG TACCACGCGA GGTGTGAGCT CCTGCCAGCA GTGCCTGGCT GTGAGCCCCA TGTGTGCCTG GTGCTCTGAT GAGGCCCTGC CTCTGGGCTC ACCTCGCTGT GACCTGAAGG AGAATCTGCT GAAGGATAAC TGTGCCCCAG AATCCATCGA GTTCCCAGTG AGTGAGGCCC GAGTACTAGA GGACAGGCCC CTCAGCGACA AGGGCTCTGG AGACAGCTCC CAGGTCACTC AAGTCAGTCC CCAGAGGATT GCACTCCGGC TCCGGCCAGA TGATTCGAAG AATTTCTCCA TCCAAGTGCG GCAGGTGGAG GATTACCCTG TGGACATCTA CTACTTGATG GACCTGTCTT ACTCCATGAA GGATGATCTG TGGAGCATCC AGAACCTGGG TACCAAGCTG GCCACCCAGA TGCGAAAGCT CACCAGTAAC CTGCGGATTG GCTTCGGGGC ATTTGTGGAC AAGCCTGTGT CACCATACAT GTATATCTCC CCACCAGAGG CCCTCGAAAA CCCCTGCTAT GATATGAAGA CCACCTGCTT GCCCATGTTT GGCTACAAAC ACGTGCTGAC GCTAACTGAC CAGGTGACCC GCTTCAATGA GGAAGTGAAG AAGCAGAGTG TGTCACGGAA CCGAGATGCC CCAGAGGGTG GCTTTGATGC CATCATGCAG GCTACAGTCT GTGATGAAAA GATTGGCTGG AGGAATGATG CATCCCACTT GCTGGTGTTT ACCACTGATG CCAAGACTCA TATAGCATTG GACGGAAGGC TGGCAGGCAT TGTCCAGCCT AATGACGGGC AGTGTCATGT TGGTAGTGAC AATCATTACT CTGCCTCCAC TACCATGGAT TATCCCTCTT TGGGGCTGAT GACTGAGAAG CTATCCCAGA AAAACATCAA TTTGATCTTT GCAGTGACTG AAAATGTAGT CAATCTCTAT CAGAACTATA GTGAGCTCAT CCCAGGGACC ACAGTTGGGG TTCTGTCCAT GGATTCCAGC AATGTCCTCC AGCTCATTGT TGATGCTTAT GGGAAAATCC GTTCTAAAGT AGAGCTGGAA GTGCGTGACC TCCCTGAAGA GTTGTCTCTA TCCTTCAATG CCACCTGCCT CAACAATGAG GTCATCCCTG GCCTCAAGTC TTGTATGGGA CTCAAGATTG GAGACACGGT GAGCTTCAGC ATTGAGGCCA AGGTGCGAGG CTGTCCCCAG GAGAAGGAGA AGTCCTTTAC CATAAAGCCC GTGGGCTTCA AGGACAGCCT GATCGTCCAG GTCACCTTTG ATTGTGACTG TGCCTGCCAG GCCCAAGCTG AACCTAATAG CCATCGCTGC AACAATGGCA ATGGGACCTT TGAGTGTGGG GTATGCCGTT GTGGGCCTGG CTGGCTGGGA TCCCAGTGTG AGTGCTCAGA GGAGGACTAT CGCCCTTCCC AGCAGGACGA ATGCAGCCCC CGGGAGGGTC AGCCCGTCTG CAGCCAGCGG GGCGAGTGCC TCTGTGGTCA ATGTGTCTGC CACAGCAGTG ACTTTGGCAA GATCACGGGC AAGTACTGCG AGTGTGACGA CTTCTCCTGT GTCCGCTACA AGGGGGAGAT GTGCTCAGGC CATGGCCAGT GCAGCTGTGG GGACTGCCTG TGTGACTCCG ACTGGACCGG CTACTACTGC AACTGTACCA CGCGTACTGA CACCTGCATG TCCAGCAATG GGCTGCTGTG CAGCGGCCGC GGCAAGTGTG AATGTGGCAG CTGTGTCTGT ATCCAGCCGG GCTCCTATGG GGACACCTGT GAGAAGTGCC CCACCTGCCC AGATGCCTGC ACCTTTAAGA AAGAATGTGT GGAGTGTAAG AAGTTTGACC GGGGAGCCCT ACATGACGAA AATACCTGCA ACCGTTACTG CCGTGACGAG ATTGAGTCAG TGAAAGAGCT TAAGGACACT GGCAAGGATG CAGTGAATTG TACCTATAAG AATGAGGATG ACTGTGTCGT CAGATTCCAG TACTATGAAG ATTCTAGTGG AAAGTCCATC CTGTATGTGG TAGAAGAGCC AGAGTGTCCC AAGGGCCCTG ACATCCTGGT GGTCCTGCTC TCAGTGATGG GGGCCATTCT GCTCATTGGC CTTGCCGCCC TGCTCATCTG GAAACTCCTC ATCACCATCC ACGACCGAAA AGAATTCGCT AAATTTGAGG AAGAACGCGC CAGAGCAAAA TGGGACACAG CCAACAACCC ACTGTATAAA GAGGCCACGT CTACCTTCAC CAATATCACG TACCGGGGCA CTTAAACCGG TCGTACGTCT CCGGATTCGA ATTCGGATCC GCGGCCGCAT AGATAACTGA TCCAGTGTGC TGGAATTAAT TCGCTGTCTG CGAGGGCCAG CTGTTGGGGT GAGTACTCCC TCTCAAAAGC GGGCATGACT TCTGCGCTAA GATTGTCAGT TTCCAAAAAC GAGGAGGATT TGATATTCAC CTGGCCCGCG GTGATGCCTT TGAGGGTGGC CGCGTCCATC TGGTCAGAAA AGACAATCTT TTTGTTGTCA AGCTTGAGGT GTGGCAGGCT TGAGATCTGG CCATACACTT GAGTGACAAT GACATCCACT TTGCCTTTCT CTCCACAGGT GTCCACTCCC AGGTCCAACT GCAGGTCGAG CATGCATCTA GGGCGGCCAA TTCCGCCCCT CTCCCCCCCC CCCCTCTCCC TCCCCCCCCC CTAACGTTAC TGGCCGAAGC CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT ATTGCCGTCT TTTGGCAATG TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT TCCTAGGGGT CTTTCCCCTC TCGCCAAAGG AATGCAAGGT CTGTTGAATG TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA GCGGAACCCC CCACCTGGCG ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC ACCTGCAAAG GCGGCACAAC CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT CAAATGGCTC TCCTCAAGCG TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA TTGTATGGGA TCTGATCTGG GGCCTCGGTG CACATGCTTT ACATGTGTTT AGTCGAGGTT AAAAAAACGT CTAGGCCCCC CGAACCACGG GGACGTGGTT TTCCTTTGAA AAACACGATG ATAAGCTTGC CACAACCCAC AAGGAGACGA CCTTCCATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGACGCC CGCCCCACGA CCCGCAGCGC CCGACCGAAA GGAGCGCACG ACCCCATGGC TCCGACCGAA GCCACCCGGG GCGGCCCCGC CGACCCCGCA CCCGCCCCCG AGGCCCACCG ACTCTAGATA ACTGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA CTTGCTTTAA AAAACCTCCC ACACCTCCCC CTGAACCTGA AACATAAAAT GAATGCAATT GTTGTTGTTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT AACGCTCGAG TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC
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