pK19mobSacB-T7 polymerase
$142.00
In stock
SKU
PL83306
T7polymerase
100μg liquid plasmid(Instantly):$163
| name | pK19mobSacB-T7 polymerase | another name | apK19mobSacB-T7 polymerase |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | protease | Prokaryotic resistance | Kan |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCAGGATTCC GTTTCCCGTC AGCTGACCGC TGATTTCATC CAGCAATGGC AGGCTGCTCA CCCGGCCGAT CAGATCACGG TGCGCGACCT GGCGGTCAAC CCGGTGCCGC ATCTGGATGC CAACCTGCTG GGTGGCTGGA TGAAACCCGA AGAACAGCGC AGCGCCGCCG AATTGGATGC CCTGGCCCGA TCCAACGAAT TGACCGATGA GTTGCTGGCA GCCGACGTGC TGGTGATGGC CGCACCGATG TACAACTTCA CCATCCCCAG CACCCTCAAG GCCTGGTTGG ACCATGTGCT GCGCGCCGGC ATCACCTTCA AGTACACGCC TACTGGCCCA CAAGGCCTGC TGACCGGCAA GCGCGCCATC GTCCTGACTG CCCGCGGCGG TATCCATGCC GGTGCCAGCA GCGACCACCA GGAACCGTAC CTGCGCCAGG TGATGGCCTT CATCGGTATT CATGATGTCG ACTTCATCCA TGCCGAAGGC CTGAACATGA GCGGCGAATT CCACGAGAAG GGCCTCAACC AGGCCAAGGC CAACCTGGCG GCGGTAGCCT GAGATTCACT GAATTCCCAA CCCTGACACC TGTTGTTGCT CCTTCGGGTG TATCTGCCCG GCCTGCATGG CCGGGTTTTT TTGCCCGGCA TTTTGTGCAG GCCAGTGCAG CCCCTACATT CCTCATGGTT GAACTCATTT GCCGCAACCC GCTAAGGTCG CGGCCTTGAT TCATTTACAC TTTATGCTTC CGGCTCGTAT AATGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGGATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCTTT GCCTGGTTTC CGGCACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC ACCAACGTGA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA ATTATTTTTG ATGGCGTCGG GATCTGATCC GGATTTACTA ACTGGAAGAG GCACTAAATG AACACGATTA ACATCGCTAA GAACGACTTC TCTGACATCG AACTGGCTGC TATCCCGTTC AACACTCTGG CTGACCATTA CGGTGAGCGT TTAGCTCGCG AACAGTTGGC CCTTGAGCAT GAGTCTTACG AGATGGGTGA AGCACGCTTC CGCAAGATGT TTGAGCGTCA ACTTAAAGCT GGTGAGGTTG CGGATAACGC TGCCGCCAAG CCTCTCATCA CTACCCTACT CCCTAAGATG ATTGCACGCA TCAACGACTG GTTTGAGGAA GTGAAAGCTA AGCGCGGCAA GCGCCCGACA GCCTTCCAGT TCCTGCAAGA AATCAAGCCG GAAGCCGTAG CGTACATCAC CATTAAGACC ACTCTGGCTT GCCTAACCAG TGCTGACAAT ACAACCGTTC AGGCTGTAGC AAGCGCAATC GGTCGGGCCA TTGAGGACGA GGCTCGCTTC GGTCGTATCC GTGACCTTGA AGCTAAGCAC TTCAAGAAAA ACGTTGAGGA ACAACTCAAC AAGCGCGTAG GGCACGTCTA CAAGAAAGCA TTTATGCAAG TTGTCGAGGC TGACATGCTC TCTAAGGGTC TACTCGGTGG CGAGGCGTGG TCTTCGTGGC ATAAGGAAGA CTCTATTCAT GTAGGAGTAC GCTGCATCGA GATGCTCATT GAGTCAACCG GAATGGTTAG CTTACACCGC CAAAATGCTG GCGTAGTAGG TCAAGACTCT GAGACTATCG AACTCGCACC TGAATACGCT GAGGCTATCG CAACCCGTGC AGGTGCGCTG GCTGGCATCT CTCCGATGTT CCAACCTTGC GTAGTTCCTC CTAAGCCGTG GACTGGCATT ACTGGTGGTG GCTATTGGGC TAACGGTCGT CGTCCTCTGG CGCTGGTGCG TACTCACAGT AAGAAAGCAC TGATGCGCTA CGAAGACGTT TACATGCCTG AGGTGTACAA AGCGATTAAC ATTGCGCAAA ACACCGCATG GAAAATCAAC AAGAAAGTCC TAGCGGTCGC CAACGTAATC ACCAAGTGGA AGCATTGTCC GGTCGAGGAC ATCCCTGCGA TTGAGCGTGA AGAACTCCCG ATGAAACCGG AAGACATCGA CATGAATCCT GAGGCTCTCA CCGCGTGGAA ACGTGCTGCC GCTGCTGTGT ACCGCAAGGA CAAGGCTCGC AAGTCTCGCC GTATCAGCCT TGAGTTCATG CTTGAGCAAG CCAATAAGTT TGCTAACCAT AAGGCCATCT GGTTCCCTTA CAACATGGAC TGGCGCGGTC GTGTTTACGC TGTGTCAATG TTCAACCCGC AAGGTAACGA TATGACCAAA GGACTGCTTA CGCTGGCGAA AGGTAAACCA ATCGGTAAGG AAGGTTACTA CTGGCTGAAA ATCCACGGTG CAAACTGTGC GGGTGTCGAT AAGGTTCCGT TCCCTGAGCG CATCAAGTTC ATTGAGGAAA ACCACGAGAA CATCATGGCT TGCGCTAAGT CTCCACTGGA GAACACTTGG TGGGCTGAGC AAGATTCTCC GTTCTGCTTC CTTGCGTTCT GCTTTGAGTA CGCTGGGGTA CAGCACCACG GCCTGAGCTA TAACTGCTCC CTTCCGCTGG CGTTTGACGG GTCTTGCTCT GGCATCCAGC ACTTCTCCGC GATGCTCCGA GATGAGGTAG GTGGTCGCGC GGTTAACTTG CTTCCTAGTG AAACCGTTCA GGACATCTAC GGGATTGTTG CTAAGAAAGT CAACGAGATT CTACAAGCAG ACGCAATCAA TGGGACCGAT AACGAAGTAG TTACCGTGAC CGATGAGAAC ACTGGTGAAA TCTCTGAGAA AGTCAAGCTG GGCACTAAGG CACTGGCTGG TCAATGGCTG GCTTACGGTG TTACTCGCAG TGTGACTAAG CGTTCAGTCA TGACGCTGGC TTACGGGTCC AAAGAGTTCG GCTTCCGTCA ACAAGTGCTG GAAGATACCA TTCAGCCAGC TATTGATTCC GGCAAGGGTC TGATGTTCAC TCAGCCGAAT CAGGCTGCTG GATACATGGC TAAGCTGATT TGGGAATCTG TGAGCGTGAC GGTGGTAGCT GCGGTTGAAG CAATGAACTG GCTTAAGTCT GCTGCTAAGC TGCTGGCTGC TGAGGTCAAA GATAAGAAGA CTGGAGAGAT TCTTCGCAAG CGTTGCGCTG TGCATTGGGT AACTCCTGAT GGTTTCCCTG TGTGGCAGGA ATACAAGAAG CCTATTCAGA CGCGCTTGAA CCTGATGTTC CTCGGTCAGT TCCGCTTACA GCCTACCATT AACACCAACA AAGATAGCGA GATTGATGCA CACAAACAGG AGTCTGGTAT CGCTCCTAAC TTTGTACACA GCCAAGACGG TAGCCACCTT CGTAAGACTG TAGTGTGGGC ACACGAGAAG TACGGAATCG AATCTTTTGC ACTGATTCAC GACTCCTTCG GTACCATTCC GGCTGACGCT GCGAACCTGT TCAAAGCAGT GCGCGAAACT ATGGTTGACA CATATGAGTC TTGTGATGTA CTGGCTGATT TCTACGACCA GTTCGCTGAC CAGTTGCACG AGTCTCAATT GGACAAAATG CCAGCACTTC CGGCTAAAGG TAACTTGAAC CTCCGTGACA TCTTAGAGTC GGACTTCGCG TTCGCGTAAG AGCAAGGAGG CTATCAATGC ACACCATCTT CGTCATCGTC GCACCGATTT TCGCCTTGAT TCTGGTCGGC TACTTGTGCC GCAAAACCAA CAAGCTGGGC GACAAGGCCG CCGCCGAAAT CAACAAGATG GTGGTCTGGC TGTGCCTGCC GGCGTTGCTG TTCAAGGTGA CGGCCACTGC CACCTGGAGC GAGATCTGGC ACCCGGGGTT CATCGCAGCC TTCGGCGCGG GGGCCCTGGC CATGTTCGTA ATCACCCTGC TGTGGCGCTT GACCAGTGGC CATGGCCTGG TGGCGTCGAG CATCGACGGG CTCAGTGCCG GGTATGCCAA CACCGGCTAT ATAGGCATCC CGTTGTGCCT GCTGCTGTTC GGCCAGGCGG GCTTGCAGCC GGCGCTGATC TCCTCTTTGA TCGTGGTGTG CCTGGTGTTT GCCATTTCCC TGACGCTGAT CGAGGTCGGG CTGCAGGAAG AACGGCAAAT CGGCCGCGCC ATCGTGGTGG TTGGCAAGGC GCTGGCGAAG AATCCGTTGG TGATCTCGCC ATTGGCGGGG GCGGCCTGGG CGGTGTCTGG CTGGGGATTG GCAGAGCCGG TGATGCATTT TCTCGACATG CTGGCGTTGG CGACCACGCC GTGCGCGCTG GTGTCGTTGG GCGCCTTCCT GGCCGAAAAG CGCCCAGGTG CGCAGGCCAG CCCATGGCCG CTGGTGGTCA TGAAACTGGT GGGGCAGCCG GCGCTGACCT GGGTGCTGGC GTACAAGGTG GACAAGGGAA AACGCAAGCG CAAAGAGAAA GCAGGTAGCT TGCAGTGGGC TTACATGGCG ATAGCTAGAC TGGGCGGTTT TATGGACAGC AAGCGAACCG GAATTGCCAG CTGGGGCGCC CTCTGGTAAG GTTGGGAAGC CCTGCAAAGT AAACTGGATG GCTTTCTTGC CGCCAAGGAT CTGATGGCGC AGGGGATCAA GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTCCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGGATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCT AGAGGATCGA TCCTTTTTAA CCCATCACAT ATACCTGCCG TTCACTATTA TTTAGTGAAA TGAGATATTA TGATATTTTC TGAATTGTGA TTAAAAAGGC AACTTTATGC CCATGCAACA GAAACTATAA AAAATACAGA GAATGAAAAG AAACAGATAG ATTTTTTAGT TCTTTAGGCC CGTAGTCTGC AAATCCTTTT ATGATTTTCT ATCAAACAAA AGAGGAAAAT AGACCAGTTG CAATCCAAAC GAGAGTCTAA TAGAATGAGG TCGAAAAGTA AATCGCGCGG GTTTGTTACT GATAAAGCAG GCAAGACCTA AAATGTGTAA AGGGCAAAGT GTATACTTTG GCGTCACCCC TTACATATTT TAGGTCTTTT TTTATTGTGC GTAACTAACT TGCCATCTTC AAACAGGAGG GCTGGAAGAA GCAGACCGCT AACACAGTAC ATAAAAAAGG AGACATGAAC GATGAACATC AAAAAGTTTG CAAAACAAGC AACAGTATTA ACCTTTACTA CCGCACTGCT GGCAGGAGGC GCAACTCAAG CGTTTGCGAA AGAAACGAAC CAAAAGCCAT ATAAGGAAAC ATACGGCATT TCCCATATTA CACGCCATGA TATGCTGCAA ATCCCTGAAC AGCAAAAAAA TGAAAAATAT CAAGTTTCTG AATTTGATTC GTCCACAATT AAAAATATCT CTTCTGCAAA AGGCCTGGAC GTTTGGGACA GCTGGCCATT ACAAAACGCT GACGGCACTG TCGCAAACTA TCACGGCTAC CACATCGTCT TTGCATTAGC CGGAGATCCT AAAAATGCGG ATGACACATC GATTTACATG TTCTATCAAA AAGTCGGCGA AACTTCTATT GACAGCTGGA AAAACGCTGG CCGCGTCTTT AAAGACAGCG ACAAATTCGA TGCAAATGAT TCTATCCTAA AAGACCAAAC ACAAGAATGG TCAGGTTCAG CCACATTTAC ATCTGACGGA AAAATCCGTT TATTCTACAC TGATTTCTCC GGTAAACATT ACGGCAAACA AACACTGACA ACTGCACAAG TTAACGTATC AGCATCAGAC AGCTCTTTGA ACATCAACGG TGTAGAGGAT TATAAATCAA TCTTTGACGG TGACGGAAAA ACGTATCAAA ATGTACAGCA GTTCATCGAT GAAGGCAACT ACAGCTCAGG CGACAACCAT ACGCTGAGAG ATCCTCACTA CGTAGAAGAT AAAGGCCACA AATACTTAGT ATTTGAAGCA AACACTGGAA CTGAAGATGG CTACCAAGGC GAAGAATCTT TATTTAACAA AGCATACTAT GGCAAAAGCA CATCATTCTT CCGTCAAGAA AGTCAAAAAC TTCTGCAAAG CGATAAAAAA CGCACGGCTG AGTTAGCAAA CGGCGCTCTC GGTATGATTG AGCTAAACGA TGATTACACA CTGAAAAAAG TGATGAAACC GCTGATTGCA TCTAACACAG TAACAGATGA AATTGAACGC GCGAACGTCT TTAAAATGAA CGGCAAATGG TACCTGTTCA CTGACTCCCG CGGATCAAAA ATGACGATTG ACGGCATTAC GTCTAACGAT ATTTACATGC TTGGTTATGT TTCTAATTCT TTAACTGGCC CATACAAGCC GCTGAACAAA ACTGGCCTTG TGTTAAAAAT GGATCTTGAT CCTAACGATG TAACCTTTAC TTACTCACAC TTCGCTGTAC CTCAAGCGAA AGGAAACAAT GTCGTGATTA CAAGCTATAT GACAAACAGA GGATTCTACG CAGACAAACA ATCAACGTTT GCGCCGAGCT TCCTGCTGAA CATCAAAGGC AAGAAAACAT CTGTTGTCAA AGACAGCATC CTTGAACAAG GACAATTAAC AGTTAACAAA TAAAAACGCA AAAGAAAATG CCGATGGGTA CCGAGCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCCT CGCGGACGTG CTCATAGTCC ACGACGCCCG TGATTTTGTA GCCCTGGCCG ACGGCCAGCA GGTAGGCCGA CAGGCTCATG CCGGCCGCCG CCGCCTTTTC CTCAATCGCT CTTCGTTCGT CTGGAAGGCA GTACACCTTG ATAGGTGGGC TGCCCTTCCT GGTTGGCTTG GTTTCATCAG CCATCCGCTT GCCCTCATCT GTTACGCCGG CGGTAGCCGG CCAGCCTCGC AGAGCAGGAT TCCCGTTGAG CACCGCCAGG TGCGAATAAG GGACAGTGAA GAAGGAACAC CCGCTCGCGG GTGGGCCTAC TTCACCTATC CTGCCCGGCT GACGCCGTTG GATACACCAA GGAAAGTCTA CACGAACCCT TTGGCAAAAT CCTGTATATC GTGCGAAAAA GGATGGATAT ACCGAAAAAA TCGCTATAAT GACCCCGAAG CAGGGTTATG CAGCGGAAAA GCGCTGCTTC CCTGCTGTTT TGTGGAATAT CTACCGACTG GAAACAGGCA AATGCAGGAA ATTACTGAAC TGAGGGGACA GGCGAGAGAC GATGCCAAAG AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT TTTCGCCAAA AGTTGGCCCA GGGCTTCCCG GTATCAACAG GGACACCAGG ATTTATTTAT TCTGCGAAGT GATCTTCCGT CACAGGTATT TATTCGGCGC AAAGTGCGTC GGGTGATGCT GCCAACTTAC TGATTTAGTG TATGATGGTG TTTTTGAGGT GCTCCAGTGG CTTCTGTTTC TATCAGCTCC TGAAAATCTC GATAACTCAA AAAATACGCC CGGTAGTGAT CTTATTTCAT TATGGTGAAA GTTGGAACCT CTTACGTGCC GATCAACGTC TCATTTTCGC CAAAAGTTGG CCCAGGGCTT CCCGGTATCA ACAGGGACAC CAGGATTTAT TTATTCTGCG AAGTGATCTT CCGTCACAGG TATTTATTCG GCGCAAAGTG CGTCGGGTGA TGCTGCCAAC TTACTGATTT AGTGTATGAT GGTGTTTTTG AGGTGCTCCA GTGGCTTCTG TTTCTATCAG GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC
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