pAX01
$128.00
In stock
SKU
PL83255
整合型木糖诱导
100μg liquid plasmid(Instantly):$163
| name | pAX01 | another name | apAX01 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Ery | Fluorescent labeling | |
| Promoter | xylA | Replicon | repB |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | rrnBT1-R：gagcgttcaccgacaaacaac | |
| 3′ sequencing primer | pAX01-R：tttatccaccgaactaagttgg | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGGGTGATGT CAAAGCTTGA AAAAACGCAC GTAACAAAAG CAAAATTTAT GCTCCATGGG GGAGACTACA ACCCCGATCA GTGGCTGGAT CGGCCCGATA TTTTAGCTGA CGATATCAAA CTGATGAAGC TTTCTCATAC GAATACGTTT TCTGTCGGCA TTTTTGCATG GAGCGCACTT GAGCCGGAGG AGGGCGTATA TCAATTTGAA TGGCTGGATG ATATTTTTGA GCGGATTCAC AGTATAGGCG GCCGGGTCAT ATTAGCAACG CCGAGCGGAG CCCGTCCGGC CTGGCTGTCG CAAACCTATC CGGAAGTTTT GCGCGTCAAT GCCTCCCGCG TCAAACAGCT GCACGGCGGA AGGCACAACC ACTGCCTCAC ATCTAAAGTC TACCGAGAAA AAACACGGCA CATCAACCGC TTATTAGCAG AACGATACGG ACATCACCCG GCGCTGTTAA TGTGGCACAT TTCAAACGAA TACGGGGGAG ATTGCCACTG TGAATCGATG CGGCCGCTCT AGAGCAACGT TCTTGCCATT GCTGCATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA TTAATTAATC ATCGGGAAGA TCTTCATCAC CGAAACGCGG CAGGCAGCTC TAGAGTTAAC AAGAGTTTGT AGAAACGCAA AAAGGCCATC CGTCAGGATG GCCTTCTGCT TAGCTAGAGC GGCGGATTTG TCCTACTCAG GAGAGCGTTC ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTCG ACTGAGCCTT TCGTTTTATT TGATGCCTCA AGCTAGAGAG TCATTACCAG ATCTCACTGC AGAGATCCCC GGGTCTAGAG TCTAGGGACC TCTTTAGCTC CTTGGAAGCT GTCAGTAGTA TACCTAATAA TTTATCTACA TTCCCTTTAG TAACGTGTAA CTTTCCAAAT TTACAAAAGC GACTCATAGA ATTATTTCCT CCCGTTAAAT AATAGATAAC TATTAAAAAT AGACAATACT TGCTCATAAG TAACGGTACT TAAATTGTTT ACTTTGGCGT GTTTCATTGC TTGATGAAAC TGATTTTTAG TAAACAGTTG ACGATATTCT CGATTGACCC ATTTTGAAAC AAAGTACGTA TATAGCTTCC AATATTTATC TGGAACATCT GTGGTATGGC GGGTAAGTTT TATTAAGACA CTGTTTACTT TTGGTTTAGG ATGAAAGCAT TCCGCTGGCA GCTTAAGCAA TTGCTGAATC GAGACTTGAG TGTGCAAGAG CAACCCTAGT GTTCGGTGAA TATCCAAGGT ACGCTTGTAG AATCCTTCTT CAACAATCAG ATAGATGTCA GACGCATGGC TTTCAAAAAC CACTTTTTTA ATAATTTGTG TGCTTAAATG GTAAGGAATA CTCCCAACAA TTTTATACCT CTGTTTGTTA GGGAATTGAA ACTGTAGAAT ATCTTGGTGA ATTAAAGTGA CACGAGTATT CAGTTTTAAT TTTTCTGACG ATAAGTTGAA TAGATGACTG TCTAATTCAA TAGACGTTAC CTGTTTACTT ATTTTAGCCA GTTTCGTCGT TAAATGCCCT TTACCTGTTC CAATTTCGTA AACGGTATCG GTTTCTTTTA AATTCAATTG TTTTATTATT TGGTTGAGTA CTTTTTCACT CGTTAAAAAG TTTTGAGAAT ATTTTATATT TTTGTTCATG TAATCACTCC TTCTTAATTA CAAATTTTTA GCATCTAATT TAACTTCAAT TCCTATTATA CAAAATTTTA AGATACTGCA CTATCAACAC ACTCTTAAGT TTGCTTCTAA GTCTTATTTC CATAACTTCT TTTACGTTTC CGCCATTCTT TGCTGTTTCG ATTTTTATGA TATGGTGCAA GTCAGCACGA ACACGAACCG TCTTATCTCC CATTATATCT TTTTTTGCAC TGATTGGTGT ATCATTTCGT TTTTCTTTTG TGCTAGAGGA TCAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTGCGGCCGC CCGCGGGAGC TCGGATCCCA TTTCCCCCTT TGATTTTTAG ATATCACTAG TTTGGACCAT TTGTCATTTC CCCCTTTGAT TTAAGTGAAC AAGTTTATCC ATCAACTATC TTAATTGAGT TAGTTTGTTT ATCCAATAAA CTAACTTTAT CTCATCATAT ACAAAATAAA TGTTTATTTC AATGTTTTTT TTAGAAAATT TAGTTATAAT ATTAGATATG ATACTTTTAA ATATCTAATT CAAGCTTCAA AAAACACCAA CTTAGTTCGG TGGATAAACA AAGGAGTGGT TATTATTCAA ATTGCAGATC AAGCTTTAGT AAAAAAAATG AATCAAAAAT TAATATTAGA TGAAATTTTG AAGAACTCCC CTGTCTCCAG GGCAACTCTC TCTGAGATTA CAGGATTAAA CAAGTCTACT GTCTCCTCTC AAGTAAATAC ACTGCTTGAA AAAGATTTTA TTTTTGAAAT TGGGGCAGGG CAATCTAGAG GCGGCAGAAG ACCTGTAATG CTTGTTTTTA ATAAGAATGC AGGCTACTCG ATTGGTATTG ATATAGGAGT CGACTATCTT AACGGAATTC TAACCGACTT AGAAGGAAAT ATTATTCTCG AGAAGACTTC TGACTTGTCT AGTTCTTCCG CTAGTGAAGT AAAAGAGATT TTATTTGCAC TTATTCATGG TTTTGTAACC CATATGCCTG AGTCCCCTTA TGGTCTAGTC GGAATAGGAA TTTGTGTTCC AGGCCTTGTA GATCGTCATC AGCAAATTAT TTTCATGCCT AACTTAAATT GGAATATCAA AGATTTGCAG TTTTTAATTG AGAGTGAGTT TAATGTTCCG GTTTTTGTTG AAAATGAAGC TAATGCAGGA GCATACGGTG AAAAAGTATT TGGTATGACA AAAAACTATG AAAACATCGT TTACATCAGT ATTAATATCG GAATTGGAAC TGGACTTGTT ATTAACAACG AATTGTATAA AGGTGTTCAG GGTTTTTCTG GGGAAATGGG TCATATGACG ATAGATTTTA ATGGACCCAA ATGCAGCTGT GGAAATCGAG GCTGTTGGGA ATTATATGCT TCTGAAAAAG CGTTACTGGC TTCGCTCTCT AAAGAAGAAA AGAATATTTC TCGAAAAGAG ATTGTGGAAC GCGCAAATAA AAATGATGTA GAAATGTTAA ATGCACTTCA AAACTTTGGC TTTTATATCG GAATTGGATT AACCAATATC CTTAATACAT TTGATATAGA AGCTGTTATC TTGAGAAATC ATATAATTGA ATCTCATCCC ATTGTTTTAA ATACGATTAA AAACGAAGTT TCTTCTAGAG TCCATTCTCA TTTAGACAAT AAATGTGAAC TATTGCCTTC TTCGTTAGGA AAAAATGCAC CTGCTTTAGG AGCGGTTTCT ATCGTTATTG ATTCTTTTTT AAGTGTTACC CCTATAAGTT AGGAGCTCCC CGGGACGTTC TTGCCATTGC TGCATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAATT AATTAATCAT CGCGACTGCA GAGATATCGA TTTCAAGCTA TATTTGGAGT TGAGCCTCTT GAAACGGACA CCCTGTATCC GAAGGATCGA AACGCTGTCA GCTACCGCAG CCAAATATAT GAAATGAAGG ATTATGCAAC CGTGATTGAT GTAAAGACAG CTTCAGTGGA AGCGGTGTAT CAAGAAGATT TTTATGCGCG CACGCCAGCG GTCACAAGCC ATGAGTATCA GCAGGGCAAG GCGTATTTTA TCGGCGCGCG TTTGGAGGAT CAATTTCAGC GTGATTTCTA TGAGGGTCTG ATCACAGACC TGTCTCTCTC TCCAGTTTTT CCGGTTCGGC ACGGAAAAGG CGTCTCCGTA CAAGCGAGGC AGGATCAGGA CAATGATTAT ATTTTTGTCA TGAATTTCAC GGAAGAAAAA CAGCTGGTCA CGTTTGATCA GAGTGTGAAG GACATAATGA CAGGAGACAT ATTGTCAGGC GACCTGACGA TGGAAAAGTA TGAAGTGAGA ATTGTCGTAA ACACACATTA GCCCATCATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTTGAT GCCTCCGTGT AAGGGGGAAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA CTAAGAAAAT GCCGTCAAAT CCGCTCGCCA TGACTTCACT AACGATGCCT TTGAAAATCT TCAAGTTCTT TTCTACTAAT TCAAGGCGTG TCTCACCAGG TTTTTGGTTT GCTCCGGCGC AAATGCAGAC AATATCAGGA T
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
