pHB201
$128.00
In stock
SKU
PL83248
100μg liquid plasmid(Instantly):$149
| name | pHB201 | another name | apHB201 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | T3：ATTAACCCTCACTAAAGGGA | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAAGCTT ACTTTATTGT TTTGCCATTT TAAAAGGTTC AAATAAAATA TTTTTCATAA AATTATCAGA TGTTTTTTAA AAAATGAAAT AAAAGAAGAA AAAAATAGAA AATACACTAA CCCAAAGAAG CGCGTAATAT CGAGGGTTTA AGAGACAATA AGAAAAAAAG ATTGAAAAAT GACATTAAAT TCTTGACAGG GAGAGATAGG TTTGATAGAA TATAATAGTT GTCGCGAGAG ACGACGTTGA AGCGCGACAA GCTAGACCAT TGAAAACTGA ATAAAGAAGA ATGACTCATG TGATGTAGCA ATACATCAAA AATCTGTCAA TCAATAATGA AAGACAAGCC ATCAATTCGG TGACTTAAAT ACTTTATTTG AGAGTTTGAT CCTCTAGAGT CGACCTGCAG GCTTTAACGT AGGCAAAGCT CAGGGTAGAC TTTGAATGGA CAGAAACATG ACATATCTCT TGAAAGGATG ATTGTGGTGG TGAAAACAGA TAAAATCTCC TCCTGAATAC AGTAAATCAC ATTCAGGAGG AGATAAAATT GTTTAAACAA ATAGACGAAA ATTATCTGCG AAAAGAGCAC TTTCACCATT ATATGACGTT AACCCGATGC TCATATAGCT TGGTGATCAA TCTAGACATC ACGAAATTGC ATGCAATATT AAAAGAAAAA AAGCTGAAAG TATATCCTGT GCAAATTTAT TTGTTAGCAA GAGCTGTGCA AAAAATTCCT GAGTTTCGGA TGGATCAAGT GAACGATGAA CTTGGTTACT GGGAGATTCT CCATCCTAGT TATACGATTC TAAATAAAGA AACAAAGACG TTTTCAAGTA TTTGGACGCC TTTTGATGAA AACTTTGCTC AGTTTTATAA AAGCTGTGTA GCCGATATTG AAACATTTAG CAAAAGCAGC AACCTATTTC CGAAACCTCA TATGCCAGAA AACATGTTCA ATATTTCAAG TCTACCGTGG ATTGATTTTA CTTCTTTTAA CCTTAATGTA TCTACAGATG AAGCTAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG CTGGAGCTCC ACCGCGGTGG CGGCCGCTCT AGAACTAGTG GATCCCCCGG GCTGCAGGAA TTCGATATCA AGCTTATCGA TACCGTCGAC CTCGAGGGGG GGCCCGGTAC CCAATTCGCC CTATAGTGAG TCGTATTACG CGCGCAATTC ACTGGCCGTC GTTTTACAAC GTCGTGACTG GGAAAACCCT GGCGTTACCC AACTTAATCG CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATTA GAGATCAATT CCCTGTTTTG GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC AGAACGCAGA AGCGGTCTGA TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC CGCCATAAAC TGCCAGGCAT CAAATTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG CGTTTCTACA AACTCTTCCT GTCGTCATAT CTACAAGCCA TCCCCCCACA GATACGGTAA ACTAGCCTCG TTTTTGCATC AGGAAAGCAG GGAATTGATC TGATGGGTAG AACCTTGGGC TTTGCCCAAA CCCACCAACG GATGGCCGTT GGATTCCAAC ATTCAATCCC CCCAACCCCC CGCATCTCCG GGGGGCTCCC TCGCCGGTTG GCGTCAAACA AACGAGTTTG TTTGAGTGCT GAAAGGGTTC GGGTAGCACT GAGCGAGTCA ACTCCTTAAT CCTCCCTACG GTCCGGTTAC CGTTGACTCA CGCCATACCC ATCGAATGGC GAATGGCGGA TCTGGAGCTG TAATATAAAA ACCTTCTTCA ACTAACGGGG CAGGTTAGTG ACATTAGAAA ACCGACTGTA AAAAGTACAG TCGGCATTAT CTCATATTAT AAAAGCCAGT CATTAGGCCT ATCTGACAAT TCCTGAATAG AGTTCATAAA CAATCCTGCA TGATAACCAT CACAAACAGA ATGATGTACC TGTAAAGATA GCGGTAAATA TATTGAATTA CCTTTATTAA TGAATTTTCC TGCTGTAATA ATGGGTAGAA GGTAATTACT ATTATTATTG ATATTTAAGT TAAACCCAGT AAATGAAGTC CATGGAATAA TAGAAAGAGA AAAAGCATTT TCAGGTATAG GTGTTTTGGG AAACAATTTC CCCGAACCAT TATATTTCTC TACATCAGAA AGGTATAAAT CATAAAACTC TTTGAAGTCA TTCTTTACAG GAGTCCAAAT ACCAGAGAAT GTTTTAGATA CACCATCAAA AATTGTATAA AGTGGCTCTA ACTTATCCCA ATAACCTAAC TCTCCGTCGC TATTGTAACC AGTTCTAAAA GCTGTATTTG AGTTTATCAC CCTTGTCACT AAGAAAATAA ATGCAGGGTA AAATTTATAT CCTTCTTGTT TTATGTTTCG GTATAAAACA CTAATATCAA TTTCTGTGGT TATACTAAAA GTCGTTTGTT GGTTCAAATA ATGATTAAAT ATCTCTTTTC TCTTCCAATT GTCTAAATCA ATTTTATTAA AGTTCATTTG ATATGCCTCC TAAATTTTTA TCTAAAGTGA ATTTAGGAGG CTTACTTGTC TGCTTTCTTC ATTAGAATCA ATCCTTTTTT AAAAGTCAAT ATTACTGTAA CATAAATATA TATTTTAAAA ATATCCCACT TTATCCAATT TTCGTTTGTT GAACTAATGG GTGCTTTAGT TGAAGAATAA AGACCACATT AAAAAATGTG GTCTTTTGTG TTTTTTTAAA GGATTTGAGC GTAGCGAAAA ATCCTTTTCT TTCTTATCTT GATAATAAGG GTAACTATTG CCGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATAAGC CGGTCTTTTT CGATTTTGGC GAAGCCGAAA TCGGGTCTTT TCTTATCTTG ATACTATATA GAAACAACAT CATTTTTCAA AATTAGGTCA AAGCCTTGTG TATCAAGGGT TTGATGGTTC TTTGACAGGT AAAAACTCCT TCTGCTATTA TTAAGGTGTC GAATCAAAAT AATAGAATGC TAGAGAACTA GCTCAGAAGG AGTTTTTTTG TTGATTTATT CATCTGAAAA TGATTATAGC ATCCTCGAAG ATAAAACCGC AACAGGTAAA AAGCGGGATT GGAAGGGGAA AAAGAGACGG ACGAACCTCA TGGCGGAGCA TTACGAAGCG TTAGAGAGTA AGATTGGGGC ACCTTACTAT GGCAAAAAGG CTGAAAAACT AATTAGTTGT GCAGAGTATC TTTCGTTTAA GAGAGACCCG GAGACGGGCA AGTTAAAACT GTATCAAGCC CATTTTTGTA AAGTGAGGTT ATGTCCGATG TGTGCGTGGC GCAGGTCGTT AAAAATTGCT TATCACAATA AGTTGATCGT AGAGGAAGCC AATAGACAGT ACGGCTGCGG ATGGATTTTT CTCACGCTGA CGATTCGAAA TGTAAAGGGA GAACGGCTGA AGCCACAAAT TTCTGCGATG ATGGAAGGCT TTAGGAAACT GTTCCAGTAC AAAAAAGTAA AAACTTCGGT TCTTGGATTT TTCAGAGCTT TAGAGATTAC CAAAAATCAT GAAGAAGATA CATATCATCC TCATTTTCAT GTGTTGATAC CAGTAAGGAA AAATTATTTT GGGAAAAACT ATATTAAGCA GGCGGAGTGG ACGAGCCTTT GGAAAAAGGC GATGAAATTG GATTACACTC CAATTGTCGA TATTCGTCGA GTGAAAGGTA AAGCTAAGAT TGACGCTGAA CAGATTGAAA ACGATGTGCG GAACGCAATG ATGGAGCAAA AAGCTGTTCT CGAAATCTCT AAATATCCGG TTAAGGATAC GGATGTTGTG CGCGGTAATA AGGTGACTGA AGACAATCTG AACACGGTGC TTTACTTGGA TGATGCGTTG GCAGCTCGAA GGTTAATTGG ATACGGTGGC ATTTTGAAGG AGATACATAA AGAGCTGAAT CTTGGTGATG CGGAGGACGG CGATCTGGTC AAGATTGAGG AAGAAGATGA CGAGGTTGCA AATGGTGCAT TTGAGGTTAT GGCTTATTGG CATCCTGGCA TTAAAAATTA CATAATCAAA TAAAAAAAGC AGACCTTTAG AAGGCCTGCT TTTTTAACTA ACCCATTTGT ATTGTGTTGA AATATGTTTT GTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGCGATTCAC AAAAAATAGG CACACGAAAA ACAAGTTAAG GGATGCAGTT TATGCATCCC TTAACTTACT TATTAAATAA TTTATAGCTA TTGAAAAGAG ATAAGAATTG TTCAAAGCTA ATATTGTTTA AATCGTCAAT TCCTGCATGT TTTAAGGAAT TGTTAAATTG ATTTTTTGTA AATATTTTCT TGTATTCTTT GTTAACCCAT TTCATAACGA AATAATTATA CTTTTGTTTA TCTTTGTGTG ATATTCTTGA TTTTTTTCTA CTTAATCTGA TAAGTGAGCT ATTCACTTTA GGTTTAGGAT GAAAATATTC TCTTGGAACC ATACTTAATA TAGAAATATC AACTTCTGCC ATTAAAAGTA ATGCCAATGA GCGTTTTGTA TTTAATAATC TTTTAGCAAA CCCGTATTCC ACGATTAAAT AAATCTCATT AGCTATACTA TCAAAAACAA TTTTGCGTAT TATATCCGTA CTTATGTTAT AAGGTATATT ACCATATATT TTATAGGATT GGTTTTTAGG AAATTTAAAC TGCAATATAT CCTTGTTTAA AACTTGGAAA TTATCGTGAT CAACAAGTTT ATTTTCTGTA GTTTTGCATA ATTTATGGTC TATTTCAATG GCAGTTACGA AATTACACCT CTTTACTAAT TCAAGGGTAA AATGGCCTTT TCCTGAGCCG ATTTCAAAGA TATTATCATG TTCATTTAAT CTTATATTTG TCATTATTTT ATCTATATTA TGTTTTGAAG TAATAAAGTT TTGACTGTGT TTTATATTTT TCTCGTTCAT TATAACCCTC TTTAATTTGG TTATATGAAT TTTGCTTATT AACGATTCAT TATAACCACT TATTTTTTGT TTGGTTGATA ATGAACTGTG CTGATTACAA AAATACTAAA AATGCCCATA TTTTTTCCTC CTTATAAAAT TAGTATAATT ATAGCACGAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTA
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
