pAD123
$128.00
In stock
SKU
PL83244
100μg liquid plasmid(Instantly):$149
| name | pAD123 | another name | apAD123 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | AmpNR：GAATAAGGGCGACACGGAAATG | |
| 3′ sequencing primer | GFP-R：CCATCTAATTCAACAAGAATTGGGACAAC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTCGAGC TCGGTACCCG GGGATCCTCT AGATTTAAGA AGGAGATATA CATATGAGTA AAGGAGAAGA ACTTTTCACT GGAGTTGTCC CAATTCTTGT TGAATTAGAT GGTGATGTTA ATGGGCACAA ATTTTCTGTC AGTGGAGAGG GTGAAGGTGA TGCAACATAC GGAAAACTTA CCCTTAAATT TATTTGCACT ACTGGAAAAC TACCTGTTCC ATGGCCAACA CTTGTCACTA CTTTCGGGTA TGGTGTTCAA TGCTTTGCGA GATACCCAGA TCATATGAAA CAGCATGACT TTTTCAAGAG TGCCATGCCC GAAGGTTATG TACAGGAAAG AACTATATTT TTCAAAGATG ACGGGAACTA CAAGACACGT GCTGAAGTCA AGTTTGAAGG TGATACCCTT GTTAATAGAA TCGAGTTAAA AGGTATTGAT TTTAAAGAAG ATGGAAACAT TCTTGGACAC AAATTGGAAT ACAACTATAA CTCACACAAT GTATACATCA TGGCAGACAA ACAAAAGAAT GGAATCAAAG TTAACTTCAA AATTAGACAC AACATTGAAG ATGGAAGCGT TCAACTAGCA GACCATTATC AACAAAATAC TCCAATTGGC GATGGCCCTG TCCTTTTACC AGACAACCAT TACCTGTCCA CACAATCTGC CCTTTCGAAA GATCCCAACG AAAAGAGAGA CCACATGGTC CTTCTTGAGT TTGTAACAGC TGCTGGGATT ACACATGGCA TGGATGAACT ATACAAATAA ATCTCCTGCA GGCATGCAAG CTTGAGTAGG ACAAATCCGC CGAGCTTCGA CGAGATTTTC AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGAACAAAG AATACAAGAA AATATTTACA AAAAATCAAT TTAACAATTC CTTAAAACAT GCAGGAATTG ACGATTTAAA CAATATTAGC TTTGAACAAT TCTTATCTCT TTTCAATAGC TATAAATTAT TTAATAAGTA AGTTAAGGGA TGCATAAACT GCATCCCTTA ACTTGTTTTT CGTGTGCCTA TTTTTTGTGA ATCGCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATACAAAA CATATTTCAA CACAATACAA ATGGGTTAGT TAAAAAAGCA GGCCTTCTAA AGGTCTGCTT TTTTTATTTG ATTATGTAAT TTTTAATGCC AGGATGCCAA TAAGCCATAA CCTCAAATGC ACCATTTGCA ACCTCGTCAT CTTCTTCCTC AATCTTGACC AGATCGCCGT CCTCCGCATC ACCAAGATTC AGCTCTTTAT GTATCTCCTT CAAAATGCCA CCGTATCCAA TTAACCTTCG AGCTGCCAAC GCATCATCCA AGTAAAGCAC CGTGTTCAGA TTGTCTTCAG TCACCTTATT ACCGCGCACA CAATCCGTAT CCTTAACCGG ATATTTAGAG ATTTCGCGAA CAGCTTTTTG CTCCATCATT GCGTTCCGCA CATCGTTTTC AATCTGTTCA GCGTCAATCT TAGCTTTACC TTTCACTCGA CGAATATCGA CAATTGGAGT GTAATCCAAT TTCATCGCCT TTTTCCAAAG GCTCGTCCAC TCCGCCTGCT TAATATAGTT TTTCCCAAAA TAATTTTTCC TTACTGGTAT CAACACATGA AAATGAGGAT GATATGTATC TTCTTCATGA TTTTTGGTAA TCTCTAAAGC TCTGAAAAAT CCAAGAACCG AAGTTTTTAC TTTTTTGTAC TGGAACAGTT TCCTAAAGCC TTCCATCATC GCAGAAATTT GTGGCTTCAG CCGTTCTCCC TTTACATTTC GAATCGTCAG CGTGAGAAAA ATCCATCCGC AGCCGTACTG TCTATTGGCT TCCTCTACGA TCAACTTATT GTGATAAGCA ATTTTTAACG ACCTGCGCCA CGCACACATC GGACATAACC TCACTTTACA AAAATGGGCT TGATACAGTT TTAACTTGCC CGTCTCCGGG TCTCTCTTAA ACGAAAGATA CTCTGCACAA CTAATTAGTT TTTCAGCCTT TTTGCCATAG TAAGGTGCCC CAATCTTACT CTCTAACGCT TCGTAATGCT CCGCCATGAG GTTCGTCCGT CTCTTTTTCC CCTTCCAATC CCGCTTTTTA CCTGTTGCGG TTTTATCTTC GAGGATGCTA TAATCATTTT CAGATGAATA AATCAACAAA AAAACTCCTT CTGAGCTAGT TCTCTAGCAT TCTATTATTT TGATTCGACA CCTTAATAAT AGCAGAAGGA GTTTTTACCT GTCAAAGAAC CATCAAACCC TTGATACACA AGGCTTTGAC CTAATTTTGA AAAATGATGT TGTTTCTATA TAGTATCAAG ATAAGAAAGA AAAGGATTTT TCGCTACGCT CAAATCCTTT AAAAAAACAC AAAAGACCAC ATTTTTTAAT GTGGTCTTTA TTCTTCAACT AAAGCACCCA TTAGTTCAAC AAACGAAAAT TGGATAAAGT GGGATATTTT TAAAATATAT ATTTATGTTA CAGTAATATT GACTTTTAAA AAAGGATTGA TTCTAATGAA GAAAGCAGAC AAGTAAGCCT CCTAAATTCA CTTTAGATAA AAATTTAGGA GGCATATCAA ATGAACTTTA ATAAAATTGA TTTAGACAAT TGGAAGAGAA AAGAGATATT TAATCATTAT TTGAACCAAC AAACGACTTT TAGTATAACC ACAGAAATTG ATATTAGTGT TTTATACCGA AACATAAAAC AAGAAGGATA TAAATTTTAC CCTGCATTTA TTTTCTTAGT GACAAGGGTG ATAAACTCAA ATACAGCTTT TAGAACTGGT TACAATAGCG ACGGAGAGTT AGGTTATTGG GATAAGTTAG AGCCACTTTA TACAATTTTT GATGGTGTAT CTAAAACATT CTCTGGTATT TGGACTCCTG TAAAGAATGA CTTCAAAGAG TTTTATGATT TATACCTTTC TGATGTAGAG AAATATAATG GTTCGGGGAA ATTGTTTCCC AAAACACCTA TACCTGAAAA TGCTTTTTCT CTTTCTATTA TTCCATGGAC TTCATTTACT GGGTTTAACT TAAATATCAA TAATAATAGT AATTACCTTC TACCCATTAT TACAGCAGGA AAATTCATTA ATAAAGGTAA TTCAATATAT TTACCGCTAT CTTTACAGGT ACATCATTCT GTTTGTGATG GTTATCATGC AGGATTGTTT ATGAACTCTA TTCAGGAATT GTCAGATAGG CCTAATGACT GGCTTTTATA ATATGAGATA ATGCCGACTG TACTTTTTAC AGTCGGTTTT CTAATGTCAC TAACCTGCCC CGTTAGTCGC CATTCGCCAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AA
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