pT2-Venus
$99.00
In stock
SKU
PL83171
pT2/Venus
100μg liquid plasmid(Instantly):$120
| name | pT2-Venus | another name | apT2-Venus |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | CAG | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pcaggs-F：GTTCGGCTTCTGGCGTGT | |
| 3′ sequencing primer | Sv40-polyA-R：GAAATTTGTGATGCTATTGC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TATACAGTTG AAGTCGGAAG TTTACATACA CCTTAGCCAA ATACATTTAA ACTCACTTTT TCACAATTCC TGACATTTAA TCCTAGTAAA AATTCCCTGT CTTAGGTCAG TTAGGATCAC CACTTTATTT TAAGAATGTG AAATATCAGA ATAATAGTAG AGAGAATGAT TCATTTCAGC TTTTATTTCT TTCATCACAT TCCCAGTGGG TCAGAAGTTT ACATACACTC AATTAGTATT TGGTAGCATT GCCTTTAAAT TGTTTAACTT GGGTCAAACA TTTCGAGTAG CCTTCCACAA GCTCTAGTTA TTAATAGTAA TCAATTACGG GGTCATTAGT TCATAGCCCA TATATGGAGT TCCGCGTTAC ATAACTTACG GTAAATGGCC CGCCTGGCTG ACCGCCCAAC GACCCCCGCC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC AATAGGGACT TTCCATTGAC GTCAATGGGT GGACTATTTA CGGTAAACTG CCCACTTGGC AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGG TCGAGGTGAG CCCCACGTTC TGCTTCACTC TCCCCATCTC CCCCCCCTCC CCACCCCCAA TTTTGTATTT ATTTATTTTT TAATTATTTT GTGCAGCGAT GGGGGCGGGG GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGTT GCCTTCGCCC CGTGCCCCGC TCCGCGCCGC CTCGCGCCGC CCGCCCCGGC TCTGACTGAC CGCGTTACTC CCACAGGTGA GCGGGCGGGA CGGCCCTTCT CCTCCGGGCT GTAATTAGCG CTTGGTTTAA TGACGGCTCG TTTCTTTTCT GTGGCTGCGT GAAAGCCTTA AAGGGCTCCG GGAGGGCCCT TTGTGCGGGG GGGAGCGGCT CGGGGGGTGC GTGCGTGTGT GTGTGCGTGG GGAGCGCCGC GTGCGGCCCG CGCTGCCCGG CGGCTGTGAG CGCTGCGGGC GCGGCGCGGG GCTTTGTGCG CTCCGCGTGT GCGCGAGGGG AGCGCGGCCG GGGGCGGTGC CCCGCGGTGC GGGGGGGCTG CGAGGGGAAC AAAGGCTGCG TGCGGGGTGT GTGCGTGGGG GGGTGAGCAG GGGGTGTGGG CGCGGCGGTC GGGCTGTAAC CCCCCCCTGC ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTGCGGGGC GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAGGGGCG CGGCGGCCCC GGAGCGCCGG CGGCTGTCGA GGCGCGGCGA GCCGCAGCCA TTGCCTTTTA TGGTAATCGT GCGAGAGGGC GCAGGGACTT CCTTTGTCCC AAATCTGGCG GAGCCGAAAT CTGGGAGGCG CCGCCGCACC CCCTCTAGCG GGCGCGGGCG AAGCGGTGCG GCGCCGGCAG GAAGGAAATG GGCGGGGAGG GCCTTCGTGC GTCGCCGCGC CGCCGTCCCC TTCTCCATCT CCAGCCTCGG GGCTGCCGCA GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC TACAGATCCT TAATTAATCT AGACCATGGT GTCCAAGGGC GAGGAGCTGT TCACCGGCGT GGTGCCCATC CTGGTGGAGC TGGACGGCGA CGTGAACGGC CACAAGTTCT CCGTGAGCGG CGAGGGCGAG GGCGACGCCA CCTACGGCAA GCTGACCCTG AAGCTGATCT GCACCACCGG CAAGCTGCCT GTGCCCTGGC CTACCCTGGT GACCACCCTG GGCTACGGCC TGCAGTGCTT CGCCAGATAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCTGAGGG CTACGTGCAG GAGAGAACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCAGAGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CAGAATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GCCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTGTA CATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCAAC TTCAAGATCA GGCACAACAT CGAGGACGGC GGCGTGCAGC TGGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCTACCAG AGCGCCCTGA GCAAGGACCC TAACGAGAAG AGGGACCACA TGGTGCTGCT GGAGTTCGTG ACCGCCGCCG GCATCACCCT GGGCATGGAC GAGCTGTACA AGTGAGCGGC CGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCCT CGAGTGCGTC CAGCTAGCAC GACTAGAGCT TCTAAAGCCA TGACATCATT TTCTGGAATT TTCCAAGCTG TTTAAAGGCA CAGTCAACTT AGTGTATGTA AACTTCTGAC CCACTGGAAT TGTGATACAG TGAATTATAA GTGAAATAAT CTGTCTGTAA ACAATTGTTG GAAAAATGAC TTGTGTCATG CACAAAGTAG ATGTCCTAAC TGACTTGCCA AAACTATTGT TTGTTAACAA GAAATTTGTG GAGTAGTTGA AAAACGAGTT TTAATGACTC CAACTTAAGT GTATGTAAAC TTCCGACTTC AACTGTATAG GGATCCGAGC TCCAATTCGC CCTATAGTGA GTCGTATTAC GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TCGAAATTAA CCCTCACTAA AGGGAACAAA AGCTGGGTAC CGGGCCCCCC CTCGATCGAG GTCGACTCTA GCTAGAGGAT CCC
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