pPICZB
$99.00
In stock
SKU
PL83119
100μg liquid plasmid(Instantly):$120
| name | pPICZB | another name | apPICZB |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Zeo |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | AOX1 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 5'AOX：GACTGGTTCCAATTGACAAGC | |
| 3′ sequencing primer | 3'AOX：GCAAATGGCATTCTGACATCC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGGCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAACG AGGAATTCAC GTGGCCCAGC CGGCCGTCTC GGATCGGTAC CTCGAGCCGC GGCGGCCGCC AGCTTTCTAG AACAAAAACT CATCTCAGAA GAGGATCTGA ATAGCGCCGT CGACCATCAT CATCATCATC ATTGAGTTTG TAGCCTTAGA CATGACTGTT CCTCAGTTCA AGTTGGGCAC TTACGAGAAG ACCGGTCTTG CTAGATTCTA ATCAAGAGGA TGTCAGAATG CCATTTGCCT GAGAGATGCA GGCTTCATTT TTGATACTTT TTTATTTGTA ACCTATATAG TATAGGATTT TTTTTGTCAT TTTGTTTCTT CTCGTACGAG CTTGCTCCTG ATCAGCCTAT CTCGCAGCTG ATGAATATCT TGTGGTAGGG GTTTGGGAAA ATCATTCGAG TTTGATGTTT TTCTTGGTAT TTCCCACTCC TCTTCAGAGT ACAGAAGATT AAGTGAGACC TTCGTTTGTG CGGATCCCCC ACACACCATA GCTTCAAAAT GTTTCTACTC CTTTTTTACT CTTCCAGATT TTCTCGGACT CCGCGCATCG CCGTACCACT TCAAAACACC CAAGCACAGC ATACTAAATT TTCCCTCTTT CTTCCTCTAG GGTGTCGTTA ATTACCCGTA CTAAAGGTTT GGAAAAGAAA AAAGAGACCG CCTCGTTTCT TTTTCTTCGT CGAAAAAGGC AATAAAAATT TTTATCACGT TTCTTTTTCT TGAAATTTTT TTTTTTAGTT TTTTTCTCTT TCAGTGACCT CCATTGATAT TTAAGTTAAT AAACGGTCTT CAATTTCTCA AGTTTCAGTT TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGT TCATTAGAAA GAAAGCATAG CAATCTAATC TAAGGGGCGG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTCCG ACGGCGGCCC ACGGGTCCCA GGCCTCGGAG ATCCGTCCCC CTTTTCCTTT GTCGATATCA TGTAATTAGT TATGTCACGC TTACATTCAC GCCCTCCCCC CACATCCGCT CTAACCGAAA AGGAAGGAGT TAGACAACCT GAAGTCTAGG TCCCTATTTA TTTTTTTATA GTTATGTTAG TATTAAGAAC GTTATTTATA TTTCAAATTT TTCTTTTTTT TCTGTACAGA CGCGTGTACG CATGTAACAT TATACTGAAA ACCTTGCTTG AGAAGGTTTT GGGACGCTCG AAGGCTTTAA TTTGCAAGCT GGAGACCAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATC
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