pGAPZA
$99.00
In stock
SKU
PL83117
100μg liquid plasmid(Instantly):$120
| name | pGAPZA | another name | apGAPZA |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Zeo |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | GAP | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 5'AOX：GACTGGTTCCAATTGACAAGC | |
| 3′ sequencing primer | 3'AOX：GCAAATGGCATTCTGACATCC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTTTTT TGTAGAAATG TCTTGGTGTC CTCGTCCAAT CAGGTAGCCA TCTCTGAAAT ATCTGGCTCC GTTGCAACTC CGAACGACCT GCTGGCAACG TAAAATTCTC CGGGGTAAAA CTTAAATGTG GAGTAATGGA ACCAGAAACG TCTCTTCCCT TCTCTCTCCT TCCACCGCCC GTTACCGTCC CTAGGAAATT TTACTCTGCT GGAGAGCTTC TTCTACGGCC CCCTTGCAGC AATGCTCTTC CCAGCATTAC GTTGCGGGTA AAACGGAGGT CGTGTACCCG ACCTAGCAGC CCAGGGATGG AAAAGTCCCG GCCGTCGCTG GCAATAATAG CGGGCGGACG CATGTCATGA GATTATTGGA AACCACCAGA ATCGAATATA AAAGGCGAAC ACCTTTCCCA ATTTTGGTTT CTCCTGACCC AAAGACTTTA AATTTAATTT ATTTGTCCCT ATTTCAATCA ATTGAACAAC TATTTCGAAA CGAGGAATTC ACGTGGCCCA GCCGGCCGTC TCGGATCGGT ACCTCGAGCC GCGGCGGCCG CCAGCTTGGG CCCGAACAAA AACTCATCTC AGAAGAGGAT CTGAATAGCG CCGTCGACCA TCATCATCAT CATCATTGAG TTTTAGCCTT AGACATGACT GTTCCTCAGT TCAAGTTGGG CACTTACGAG AAGACCGGTC TTGCTAGATT CTAATCAAGA GGATGTCAGA ATGCCATTTG CCTGAGAGAT GCAGGCTTCA TTTTTGATAC TTTTTTATTT GTAACCTATA TAGTATAGGA TTTTTTTTGT CATTTTGTTT CTTCTCGTAC GAGCTTGCTC CTGATCAGCC TATCTCGCAG CTGATGAATA TCTTGTGGTA GGGGTTTGGG AAAATCATTC GAGTTTGATG TTTTTCTTGG TATTTCCCAC TCCTCTTCAG AGTACAGAAG ATTAAGTGAG ACCTTCGTTT GTGCGGATCC CCCACACACC ATAGCTTCAA AATGTTTCTA CTCCTTTTTT ACTCTTCCAG ATTTTCTCGG ACTCCGCGCA TCGCCGTACC ACTTCAAAAC ACCCAAGCAC AGCATACTAA ATTTTCCCTC TTTCTTCCTC TAGGGTGTCG TTAATTACCC GTACTAAAGG TTTGGAAAAG AAAAAAGAGA CCGCCTCGTT TCTTTTTCTT CGTCGAAAAA GGCAATAAAA ATTTTTATCA CGTTTCTTTT TCTTGAAATT TTTTTTTTTA GTTTTTTTCT CTTTCAGTGA CCTCCATTGA TATTTAAGTT AATAAACGGT CTTCAATTTC TCAAGTTTCA GTTTCATTTT TCTTGTTCTA TTACAACTTT TTTTACTTCT TGTTCATTAG AAAGAAAGCA TAGCAATCTA ATCTAAGGGC GGTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTC CGACGGCGGC CCACGGGTCC CAGGCCTCGG AGATCCGTCC CCCTTTTCCT TTGTCGATAT CATGTAATTA GTTATGTCAC GCTTACATTC ACGCCCTCCC CCCACATCCG CTCTAACCGA AAAGGAAGGA GTTAGACAAC CTGAAGTCTA GGTCCCTATT TATTTTTTTA TAGTTATGTT AGTATTAAGA ACGTTATTTA TATTTCAAAT TTTTCTTTTT TTTCTGTACA GACGCGTGTA CGCATGTAAC ATTATACTGA AAACCTTGCT TGAGAAGGTT TTGGGACGCT CGAAGGCTTT AATTTGCAAG CTGGAGACCA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGCATGA GATC
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