pROKII
$99.00
In stock
SKU
PL83082
100μg liquid plasmid(Instantly):$134
| name | pROKII | another name | apROKII |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | 35s | Replicon | oriV |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 35s： | |
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA GGATCCCCGG GTACCGAGCT CGAATTTCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGAACCACCA TCAAACAGGA TTTTCGCCTG CTGGGGCAAA CCAGCGTGGA CCGCTTGCTG CAACTCTCTC AGGGCCAGGC GGTGAAGGGC AATCAGCTGT TGCCCGTCTC ACTGGTGAAA AGAAAAACCA CCCCAGTACA TTAAAAACGT CCGCAATGTG TTATTAAGTT GTCTAAGCGT CAATTTGTTT ACACCACAAT ATATCCTGCC ACCAGCCAGC CAACAGCTCC CCGACCGGCA GCTCGGCACA AAATCACCAC TCGATACAGG CAGCCCATCA GTCCGGGACG GCGTCAGCGG GAGAGCCGTT GTAAGGCGGC AGACTTTGCT CATGTTACCG ATGCTATTCG GAAGAACGGC AACTAAGCTG CCGGGTTTGA AACACGGATG ATCTCGCGGA GGGTAGCATG TTGATTGTAA CGATGACAGA GCGTTGCTGC CTGTGATCAA ATATCATCTC CCTCGCAGAG ATCCGAATTA TCAGCCTTCT TATTCATTTC TCGCTTAACC GTGACAGGCT GTCGATCTTG AGAACTATGC CGACATAATA GGAAATCGCT GGATAAAGCC GCTGAGGAAG CTGAGTGGCG CTATTTCTTT AGAAGTGAAC GTTGACGATA TCAACTCCCC TATCCATTGC TCACCGAATG GTACAGGTCG GGGACCCGAA GTTCCGACTG TCGGCCTGAT GCATCCCCGG CTGATCGACC CCAGATCTGG GGCTGAGAAA GCCCAGTAAG GAAACAACTG TAGGTTCGAG TCGCGAGATC CCCCGGAACC AAAGGAAGTA GGTTAAACCC GCTCCGATCA GGCCGAGCCA CGCCAGGCCG AGAACATTGG TTCCTGTAGG CATCGGGATT GGCGGATCAA ACACTAAAGC TACTGGAACG AGCAGAAGTC CTCCGGCCGC CAGTTGCCAG GCGGTAAAGG TGAGCAGAGG CACGGGAGGT TGCCACTTGC GGGTCAGCAC GGTTCCGAAC GCCATGGAAA CCGCCCCCGC CAGGCCCGCT GCGACGCCGA CAGGATCTAG CGCTGCGTTT GGTGTCAACA CCAACAGCGC CACGCCCGCA GTTCCGCAAA TAGCCCCCAG GACCGCCATC AATCGTATCG GGCTACCTAG CAGAGCGGCA GAGATGAACA CGACCATCAG CGGCTGCACA GCGCCTACCG TCGCCGCGAC CCCGCCCGGC AGGCGGTAGA CCGAAATAAA CAACAAGCTC CAGAATAGCG AAATATTAAG TGCGCCGAGG ATGAAGATGC GCATCCACCA GATTCCCGTT GGAATCTGTC GGACGATCAT CACGAGCAAT AAACCCGCCG GCAACGCCCG CAGCAGCATA CCGGCGACCC CTCGGCCTCG CTGTTCGGGC TCCACGAAAA CGCCGGACAG ATGCGCCTTG TGAGCGTCCT TGGGGCCGTC CTCCTGTTTG AAGACCGACA GCCCAATGAT CTCGCCGTCG ATGTAGGCGC CGAATGCCAC GGCATCTCGC AACCGTTCAG CGAACGCCTC CATGGGCTTT TTCTCCTCGT GCTCGTAAAC GGACCCGAAC ATCTCTGGAG CTTTCTTCAG GGCCGACAAT CGGATCTCGC GGAAATCCTG CACGTCGGCC GCTCCAAGCC GTCGAATCTG AGCCTTAATC ACAATTGTCA ATTTTAATCC TCTGTTTATC GGCAGTTCGT AGAGCGCGCC GTGCGTCCCG AGCGATACTG AGCGAAGCAA GTGCGTCGAG CAGTGCCCGC TTGTTCCTGA AATGCCAGTA AAGCGCTGGC TGCTGAACCC CCAGCCGGAA CTGACCCCAC AAGGCCCTAG CGTTTGCAAT GCACCAGGTC ATCATTGACC CAGGCGTGTT CCACCAGGCC GCTGCCTCGC AACTCTTCGC AGGCTTCGCC GACCTGCTCG CGCCACTTCT TCACGCGGGT GGAATCCGAT CCGCACATGA GGCGGAAGGT TTCCAGCTTG AGCGGGTACG GCTCCCGGTG CGAGCTGAAA TAGTCGAACA TCCGTCGGGC CGTCGGCGAC AGCTTGCGGT ACTTCTCCCA TATGAATTTC GTGTAGTGGT CGCCAGCAAA CAGCACGACG ATTTCCTCGT CGATCAGGAC CTGGCAACGG GACGTTTTCT TGCCACGGTC CAGGACGCGG AAGCGGTGCA GCAGCGACAC CGATTCCAGG TGCCCAACGC GGTCGGACGT GAAGCCCATC GCCGTCGCCT GTAGGCGCGA CAGGCATTCC TCGGCCTTCG TGTAATACCG GCCATTGATC GACCAGCCCA GGTCCTGGCA AAGCTCGTAG AACGTGAAGG TGATCGGCTC GCCGATAGGG GTGCGCTTCG CGTACTCCAA CACCTGCTGC CACACCAGTT CGTCATCGTC GGCCCGCAGC TCGACGCCGG TGTAGGTGAT CTTCACGTCC TTGTTGACGT GGAAAATGAC CTTGTTTTGC AGCGCCTCGC GCGGGATTTT CTTGTTGCGC GTGGTGAACA GGGCAGAGCG GGCCGTGTCG TTTGGCATCG CTCGCATCGT GTCCGGCCAC GGCGCAATAT CGAACAAGGA AAGCTGCATT TCCTTGATCT GCTGCTTCGT GTGTTTCAGC AACGCGGCCT GCTTGGCCTC GCTGACCTGT TTTGCCAGGT CCTCGCCGGC GGTTTTTCGC TTCTTGGTCG TCATAGTTCC TCGCGTGTCG ATGGTCATCG ACTTCGCCAA ACCTGCCGCC TCCTGTTCGA GACGACGCGA ACGCTCCACG GCGGCCGATG GCGCGGGCAG GGCAGGGGGA GCCAGTTGCA CGCTGTCGCG CTCGATCTTG GCCGTAGCTT GCTGGACCAT CGAGCCGACG GACTGGAAGG TTTCGCGGGG CGCACGCATG ACGGTGCGGC TTGCGATGGT TTCGGCATCC TCGGCGGAAA ACCCCGCGTC GATCAGTTCT TGCCTGTATG CCTTCCGGTC AAACGTCCGA TTCATTCACC CTCCTTGCGG GATTGCCCCG ACTCACGCCG GGGCAATGTG CCCTTATTCC TGATTTGACC CGCCTGGTGC CTTGGTGTCC AGATAATCCA CCTTATCGGC AATGAAGTCG GTCCCGTAGA CCGTCTGGCC GTCCTTCTCG TACTTGGTAT TCCGAATCTT GCCCTGCACG AATACCAGCG ACCCCTTGCC CAAATACTTG CCGTGGGCCT CGGCCTGAGA GCCAAAACAC TTGATGCGGA AGAAGTCGGT GCGCTCCTGC TTGTCGCCGG CATCGTTGCG CCACATCTAG GTACTAAAAC AATTCATCCA GTAAAATATA ATATTTTATT TTCTCCCAAT CAGGCTTGAT CCCCAGTAAG TCAAAAAATA GCTCGACATA CTGTTCTTCC CCGATATCCT CCCTGATCGA CCGGACGCAG AAGGCAATGT CATACCACTT GTCCGCCCTG CCGCTTCTCC CAAGATCAAT AAAGCCACTT ACTTTGCCAT CTTTCACAAA GATGTTGCTG TCTCCCAGGT CGCCGTGGGA AAAGACAAGT TCCTCTTCGG GCTTTTCCGT CTTTAAAAAA TCATACAGCT CGCGCGGATC TTTAAATGGA GTGTCTTCTT CCCAGTTTTC GCAATCCACA TCGGCCAGAT CGTTATTCAG TAAGTAATCC AATTCGGCTA AGCGGCTGTC TAAGCTATTC GTATAGGGAC AATCCGATAT GTCGATGGAG TGAAAGAGCC TGATGCACTC CGCATACAGC TCGATAATCT TTTCAGGGCT TTGTTCATCT TCATACTCTT CCGAGCAAAG GACGCCATCG GCCTCACTCA TGAGCAGATT GCTCCAGCCA TCATGCCGTT CAAAGTGCAG GACCTTTGGA ACAGGCAGCT TTCCTTCCAG CCATAGCATC ATGTCCTTTT CCCGTTCCAC ATCATAGGTG GTCCCTTTAT ACCGGCTGTC CGTCATTTTT AAATATAGGT TTTCATTTTC TCCCACCAGC TTATATACCT TAGCAGGAGA CATTCCTTCC GTATCTTTTA CGCAGCGGTA TTTTTCGATC AGTTTTTTCA ATTCCGGTGA TATTCTCATT TTAGCCATTT ATTATTTCCT TCCTCTTTTC TACAGTATTT AAAGATACCC CAAGAAGCTA ATTATAACAA GACGAACTCC AATTCACTGT TCCTTGCATT CTAAAACCTT AAATACCAGA AAACAGCTTT TTCAAAGTTG TTTTCAAAGT TGGCGTATAA CATAGTATCG ACGGAGCCGA TTTTGAAACC ACAATTATGG GTGATGCTGC CAACTTACTG ATTTAGTGTA TGATGGTGTT TTTGAGGTGC TCCAGTGGCT TCTGTGTCTA TCAGCTGTCC CTCCTGTTCA GCTACTGACG GGGTGGTGCG TAACGGCAAA AGCACCGCCG GACATCAGCG CTATCTCTGC TCTCACTGCC GTAAAACATG GCAACTGCAG TTCACTTACA CCGCTTCTCA ACCCGGTACG CACCAGAAAA TCATTGATAT GGCCATGAAT GGCGTTGGAT GCCGGGCAAC AGCCCGCATT ATGGGCGTTG GCCTCAACAC GATTTTACGT CACTTAAAAA ACTCAGGCCG CAGTCGGTAA CCTCGCGCAT ACAGCCGGGC AGTGACGTCA TCGTCTGCGC GGAAATGGAC GAACAGTGGG GCTATGTCGG GGCTAAATCG CGCCAGCGCT GGCTGTTTTA CGCGTATGAC AGTCTCCGGA AGACGGTTGT TGCGCACGTA TTCGGTGAAC GCACTATGGC GACGCTGGGG CGTCTTATGA GCCTGCTGTC ACCCTTTGAC GTGGTGATAT GGATGACGGA TGGCTGGCCG CTGTATGAAT CCCGCCTGAA GGGAAAGCTG CACGTAATCA GCAAGCGATA TACGCAGCGA ATTGAGCGGC ATAACCTGAA TCTGAGGCAG CACCTGGCAC GGCTGGGACG GAAGTCGCTG TCGTTCTCAA AATCGGTGGA GCTGCATGAC AAAGTCATCG GGCATTATCT GAACATAAAA CACTATCAAT AAGTTGGAGT CATTACCCAA TTATGATAGA ATTTACAAGC TATAAGGTTA TTGTCCTGGG TTTCAAGCAT TAGTCCATGC AAGTTTTTAT GCTTTGCCCA TTCTATAGAT ATATTGATAA GCGCGCTGCC TATGCCTTGC CCCCTGAAAT CCTTACATAC GGCGATATCT TCTATATAAA AGATATATTA TCTTATCAGT ATTGTCAATA TATTCAAGGC AATCTGCCTC CTCATCCTCT TCATCCTCTT CGTCTTGGTA GCTTTTTAAA TATGGCGCTT CATAGAGTAA TTCTGTAAAG GTCCAATTCT CGTTTCATAC CTCGGTATAA TCTTACCTAT CACCTCAAAT GGTTCGCTGG GTTTATCGCA CCCCCGAACA CGAGCACGGC ACCCGCGACC ACTATGCCAA GAATGCCCAA GGTAAAAATT GCCGGCCCCG CCATGAAGTC CGTGAATGCC CCGACGGCCG AAGTGAAGGG CAGGCCGCCA CCAGGCCGCC GCCCTCACTG CCCGGCACCT GGTCGCTGAA TGTCGATGCC AGCACCTGCG GCACGTCAAT GCTTCCGGGC GTCGCGCTCG GGCTGATCGC CCATCCCGTT ACTGCCCCGA TCCCGGCAAT GGCAAGGACT GCCAGCGCTG CCATTTTTGG GGTGAGGCCG TTCGCGGCCG AGGGGCGCAG CCCCTGGGGG GATGGGAGGC CCGCGTTAGC GGGCCGGGAG GGTTCGAGAA GGGGGGGCAC CCCCCTTCGG CGTGCGCGGT CACGCGCACA GGGCGCAGCC CTGGTTAAAA ACAAGGTTTA TAAATATTGG TTTAAAAGCA GGTTAAAAGA CAGGTTAGCG GTGGCCGAAA AACGGGCGGA AACCCTTGCA AATGCTGGAT TTTCTGCCTG TGGACAGCCC CTCAAATGTC AATAGGTGCG CCCCTCATCT GTCAGCACTC TGCCCCTCAA GTGTCAAGGA TCGCGCCCCT ATCTGTCAGT AGTCGCGCCC CTCAAGTGTC AATACCGCAG GGCACTTATC CCCAGGCTTG TCCACATCAT CTGTGGGAAA CTCGCGTAAA ATCAGGCGTT TTCGCCGATT TGCGAGGCTG GCCAGCTCCA CGTCGCCGGC CGAAATCGAG CCTGCCCCTC ATCTGTCAAC GCCGCGCCGG GTGAGTCGGC CCCTCAAGTG TCAACGTCCG CCCCTCATCT GTCAGTGAGG GCCAAGTTTT CCGCGAGGTA TCCACAACGC CGGCGGCCGC GGTGTCTCGC ACACGGCTTC GACGGCGTTT CTGGCGCGTT TGCAGGGCCA TAGACGGCCG CCAGCCCAGC GGCGAGGGCA ACCAGCCCGG
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