pESC-LEU
$128.00
In stock
SKU
PL83074
100μg liquid plasmid(Instantly):$149
| name | pESC-LEU | another name | apESC-LEU |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Multiple frames unloaded | Prokaryotic resistance | Amp |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | GAL1,10 | Replicon | 2U |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | GAL1-F：ATTTTCGGTTTGTATTACTTC | |
| 3′ sequencing primer | GALI-R：GTTCTTAATACTAACATAACT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATCGA CTACGTCGTA AGGCCGTTTC TGACAGAGTA AAATTCTTGA GGGAACTTTC ACCATTATGG GAAATGCTTC AAGAAGGTAT TGACTTAAAC TCCATCAAAT GGTCAGGTCA TTGAGTGTTT TTTATTTGTT GTATTTTTTT TTTTTTAGAG AAAATCCTCC AATATCAAAT TAGGAATCGT AGTTTCATGA TTTTCTGTTA CACCTAACTT TTTGTGTGGT GCCCTCCTCC TTGTCAATAT TAATGTTAAA GTGCAATTCT TTTTCCTTAT CACGTTGAGC CATTAGTATC AATTTGCTTA CCTGTATTCC TTTACTATCC TCCTTTTTCT CCTTCTTGAT AAATGTATGT AGATTGCGTA TATAGTTTCG TCTACCCTAT GAACATATTC CATTTTGTAA TTTCGTGTCG TTTCTATTAT GAATTTCATT TATAAAGTTT ATGTACAAAT ATCATAAAAA AAGAGAATCT TTTTAAGCAA GGATTTTCTT AACTTCTTCG GCGACAGCAT CACCGACTTC GGTGGTACTG TTGGAACCAC CTAAATCACC AGTTCTGATA CCTGCATCCA AAACCTTTTT AACTGCATCT TCAATGGCCT TACCTTCTTC AGGCAAGTTC AATGACAATT TCAACATCAT TGCAGCAGAC AAGATAGTGG CGATAGGGTC AACCTTATTC TTTGGCAAAT CTGGAGCAGA ACCGTGGCAT GGTTCGTACA AACCAAATGC GGTGTTCTTG TCTGGCAAAG AGGCCAAGGA CGCAGATGGC AACAAACCCA AGGAACCTGG GATAACGGAG GCTTCATCGG AGATGATATC ACCAAACATG TTGCTGGTGA TTATAATACC ATTTAGGTGG GTTGGGTTCT TAACTAGGAT CATGGCGGCA GAATCAATCA ATTGATGTTG AACCTTCAAT GTAGGGAATT CGTTCTTGAT GGTTTCCTCC ACAGTTTTTC TCCATAATCT TGAAGAGGCC AAAAGATTAG CTTTATCCAA GGACCAAATA GGCAATGGTG GCTCATGTTG TAGGGCCATG AAAGCGGCCA TTCTTGTGAT TCTTTGCACT TCTGGAACGG TGTATTGTTC ACTATCCCAA GCGACACCAT CACCATCGTC TTCCTTTCTC TTACCAAAGT AAATACCTCC CACTAATTCT CTGACAACAA CGAAGTCAGT ACCTTTAGCA AATTGTGGCT TGATTGGAGA TAAGTCTAAA AGAGAGTCGG ATGCAAAGTT ACATGGTCTT AAGTTGGCGT ACAATTGAAG TTCTTTACGG ATTTTTAGTA AACCTTGTTC AGGTCTAACA CTACCGGTAC CCCATTTAGG ACCAGCCACA GCACCTAACA AAACGGCATC AACCTTCTTG GAGGCTTCCA GCGCCTCATC TGGAAGTGGG ACACCTGTAG CATCGATAGC AGCACCACCA ATTAAATGAT TTTCGAAATC GAACTTGACA TTGGAACGAA CATCAGAAAT AGCTTTAAGA ACCTTAATGG CTTCGGCTGT GATTTCTTGA CCAACGTGGT CACCTGGCAA AACGACGATC TTCTTAGGGG CAGACATAGG GGCAGACATT AGAATGGTAT ATCCTTGAAA TATATATATA TATTGCTGAA ATGTAAAAGG TAAGAAAAGT TAGAAAGTAA GACGATTGCT AACCACCTAT TGGAAAAAAC AATAGGTCCT TAAATAATAT TGTCAACTTC AAGTATTGTG ATGCAAGCAT TTAGTCATGA ACGCTTCTCT ATTCTATATG AAAAGCCGGT TCCGGCCTCT CACCTTTCCT TTTTCTCCCA ATTTTTCAGT TGAAAAAGGT ATATGCGTCA GGCGACCTCT GAAATTAACA AAAAATTTCC AGTCATCGAA TTTGATTCTG TGCGATAGCG CCCCTGTGTG TTCTCGTTAT GTTGAGGAAA AAAATAATGG TTGCTAAGAG ATTCGAACTC TTGCATCTTA CGATACCTGA GTATTCCCAC AGTTAACTGC GGTCAAGATA TTTCTTGAAT CAGGCGCCTT AGACCGCTCG GCCAAACAAC CAATTACTTG TTGAGAAATA GAGTATAATT ATCCTATAAA TATAACGTTT TTGAACACAC ATGAACAAGG AAGTACAGGA CAATTGATTT TGAAGAGAAT GTGGATTTTG ATGTAATTGT TGGGATTCCA TTTTTAATAA GGCAATAATA TTAGGTATGT GGATATACTA GAAGTTCTCC TCGACCGTCG ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT AAACGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGAATT GGAGCGACCT CATGCTATAC CTGAGAAAGC AACCTGACCT ACAGGAAAGA GTTACTCAAG AATAAGAATT TTCGTTTTAA AACCTAAGAG TCACTTTAAA ATTTGTATAC ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA TTTAGAAGTG TCAACAACGT ATCTACCAAC GATTTGACCC TTTTCCATCT TTTCGTAAAT TTCTGGCAAG GTAGACAAGC CGACAACCTT GATTGGAGAC TTGACCAAAC CTCTGGCGAA GAATTGTTAA TTAAGAGCTC AGATCTTATC GTCGTCATCC TTGTAATCCA TCGATACTAG TGCGGCCGCC CTTTAGTGAG GGTTGAATTC GAATTTTCAA AAATTCTTAC TTTTTTTTTG GATGGACGCA AAGAAGTTTA ATAATCATAT TACATGGCAT TACCACCATA TACATATCCA TATACATATC CATATCTAAT CTTACTTATA TGTTGTGGAA ATGTAAAGAG CCCCATTATC TTAGCCTAAA AAAACCTTCT CTTTGGAACT TTCAGTAATA CGCTTAACTG CTCATTGCTA TATTGAAGTA CGGATTAGAA GCCGCCGAGC GGGTGACAGC CCTCCGAAGG AAGACTCTCC TCCGTGCGTC CTCGTCTTCA CCGGTCGCGT TCCTGAAACG CAGATGTGCC TCGCGCCGCA CTGCTCCGAA CAATAAAGAT TCTACAATAC TAGCTTTTAT GGTTATGAAG AGGAAAAATT GGCAGTAACC TGGCCCCACA AACCTTCAAA TGAACGAATC AAATTAACAA CCATAGGATG ATAATGCGAT TAGTTTTTTA GCCTTATTTC TGGGGTAATT AATCAGCGAA GCGATGATTT TTGATCTATT AACAGATATA TAAATGCAAA AACTGCATAA CCACTTTAAC TAATACTTTC AACATTTTCG GTTTGTATTA CTTCTTATTC AAATGTAATA AAAGTATCAA CAAAAAATTG TTAATATACC TCTATACTTT AACGTCAAGG AGAAAAAACC CCGGATCCGT AATACGACTC ACTATAGGGC CCGGGCGTCG ACATGGAACA GAAGTTGATT TCCGAAGAAG ACCTCGAGTA AGCTTGGTAC CGCGGCTAGC TAAGATCCGC TCTAACCGAA AAGGAAGGAG TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGATCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAAC GAAGCATCTG TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC CAACGAAGAA TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA AAGAATCTAT ACTTCTTTTT TGTTCTACAA AAATGCATCC CGAGAGCGCT ATTTTTCTAA CAAAGCATCT TAGATTACTT TTTTTCTCCT TTGTGCGCTC TATAATGCAG TCTCTTGATA ACTTTTTGCA CTGTAGGTCC GTTAAGGTTA GAAGAAGGCT ACTTTGGTGT CTATTTTCTC TTCCATAAAA AAAGCCTGAC TCCACTTCCC GCGTTTACTG ATTACTAGCG AAGCTGCGGG TGCATTTTTT CAAGATAAAG GCATCCCCGA TTATATTCTA TACCGATGTG GATTGCGCAT ACTTTGTGAA CAGAAAGTGA TAGCGTTGAT GATTCTTCAT TGGTCAGAAA ATTATGAACG GTTTCTTCTA TTTTGTCTCT ATATACTACG TATAGGAAAT GTTTACATTT TCGTATTGTT TTCGATTCAC TCTATGAATA GTTCTTACTA CAATTTTTTT GTCTAAAGAG TAATACTAGA GATAAACATA AAAAATGTAG AGGTCGAGTT TAGATGCAAG TTCAAGGAGC GAAAGGTGGA TGGGTAGGTT ATATAGGGAT ATAGCACAGA GATATATAGC AAAGAGATAC TTTTGAGCAA TGTTTGTGGA AGCGGTATTC GCAATATTTT AGTAGCTCGT TACAGTCCGG TGCGTTTTTG GTTTTTTGAA AGTGCGTCTT CAGAGCGCTT TTGGTTTTCA AAAGCGCTCT GAAGTTCCTA TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCAA AGCGTTTCCG AAAACGAGCG CTTCCGAAAA TGCAACGCGA GCTGCGCACA TACAGCTCAC TGTTCACGTC GCACCTATAT CTGCGTGTTG CCTGTATATA TATATACATG AGAAGAACGG CATAGTGCGT GTTTATGCTT AAATGCGTAC TTATATGCGT CTATTTATGT AGGATGAAAG GTAGTCTAGT ACCTCCTGTG ATATTATCCC ATTCCATGCG GGGTATCGTA TGCTTCCTTC AGCACTACCC TTTAGCTGTT CTATATGCTG CCACTCCTCA ATTGGATTAG TCTCATCCTT CAATGCTATC ATTTCCTTTG ATATTGGATC ATACTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTC
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