pShuttle-CMV
$99.00
In stock
SKU
PL83068
100μg liquid plasmid(Instantly):$120
| name | pShuttle-CMV | another name | apShuttle-CMV |
| Insert Size (bp) | Plasmid host | Mammalian cell,Adenovirus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Sv40-polyA-R：GAAATTTGTGATGCTATTGC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCATTCGC CATTCAGGAT CGAATTAATT CTTAATTAAC ATCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ACTGTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGAGATCTGG TACCGTCGAC GCGGCCGCTC GAGCCTAAGC TTCTAGATAA GATATCCGAT CCACCGGATC TAGATAACTG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAACG CGGATCTGGG CGTGGTTAAG GGTGGGAAAG AATATATAAG GTGGGGGTCT TATGTAGTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA ACTCGTTTGA TGGAAGCATT GTGAGCTCAT ATTTGACAAC GCGCATGCCC CCATGGGCCG GGGTGCGTCA GAATGTGATG GGCTCCAGCA TTGATGGTCG CCCCGTCCTG CCCGCAAACT CTACTACCTT GACCTACGAG ACCGTGTCTG GAACGCCGTT GGAGACTGCA GCCTCCGCCG CCGCTTCAGC CGCTGCAGCC ACCGCCCGCG GGATTGTGAC TGACTTTGCT TTCCTGAGCC CGCTTGCAAG CAGTGCAGCT TCCCGTTCAT CCGCCCGCGA TGACAAGTTG ACGGCTCTTT TGGCACAATT GGATTCTTTG ACCCGGGAAC TTAATGTCGT TTCTCAGCAG CTGTTGGATC TGCGCCAGCA GGTTTCTGCC CTGAAGGCTT CCTCCCCTCC CAATGCGGTT TAAAACATAA ATAAAAAACC AGACTCTGTT TGGATTTGGA TCAAGCAAGT GTCTTGCTGT CTTTATTTAG GGGTTTTGCG CGCGCGGTAG GCCCGGGACC AGCGGTCTCG GTCGTTGAGG GTCCTGTGTA TTTTTTCCAG GACGTGGTAA AGGTGACTCT GGATGTTCAG ATACATGGGC ATAAGCCCGT CTCTGGGGTG GAGGTAGCAC CACTGCAGAG CTTCATGCTG CGGGGTGGTG TTGTAGATGA TCCAGTCGTA GCAGGAGCGC TGGGCGTGGT GCCTAAAAAT GTCTTTCAGT AGCAAGCTGA TTGCCAGGGG CAGGCCCTTG GTGTAAGTGT TTACAAAGCG GTTAAGCTGG GATGGGTGCA TACGTGGGGA TATGAGATGC ATCTTGGACT GTATTTTTAG GTTGGCTATG TTCCCAGCCA TATCCCTCCG GGGATTCATG TTGTGCAGAA CCACCAGCAC AGTGTATCCG GTGCACTTGG GAAATTTGTC ATGTAGCTTA GAAGGAAATG CGTGGAAGAA CTTGGAGACG CCCTTGTGAC CTCCAAGATT TTCCATGCAT TCGTCCATAA TGATGGCAAT GGGCCCACGG GCGGCGGCCT GGGCGAAGAT ATTTCTGGGA TCACTAACGT CATAGTTGTG TTCCAGGATG AGATCGTCAT AGGCCATTTT TACAAAGCGC GGGCGGAGGG TGCCAGACTG CGGTATAATG GTTCCATCCG GCCCAGGGGC GTAGTTACCC TCACAGATTT GCATTTCCCA CGCTTTGAGT TCAGATGGGG GGATCATGTC TACCTGCGGG GCGATGAAGA AAACGGTTTC CGGGGTAGGG GAGATCAGCT GGGAAGAAAG CAGGTTCCTG AGCAGCTGCG ACTTACCGCA GCCGGTGGGC CCGTAAATCA CACCTATTAC CGGCTGCAAC TGGTAGTTAA GAGAGCTGCA GCTGCCGTCA TCCCTGAGCA GGGGGGCCAC TTCGTTAAGC ATGTCCCTGA CTCGCATGTT TTCCCTGACC AAATCCGCCA GAAGGCGCTC GCCGCCCAGC GATAGCAGTT CTTGCAAGGA AGCAAAGTTT TTCAACGGTT TGAGACCGTC CGCCGTAGGC ATGCTTTTGA GCGTTTGACC AAGCAGTTCC AGGCGGTCCC ACAGCTCGGT CACCTGCTCT ACGGCATCTC GATCCAGCAT ATCTCCTCGT TTCGCGGGTT GGGGCGGCTT TCGCTGTACG GCAGTAGTCG GTGCTCGTCC AGACGGGCCA GGGTCATGTC TTTCCACGGG CGCAGGGTCC TCGTCAGCGT AGTCTGGGTC ACGGTGAAGG GGTGCGCTCC GGGCTGCGCG CTGGCCAGGG TGCGCTTGAG GCTGGTCCTG CTGGTGCTGA AGCGCTGCCG GTCTTCGCCC TGCGCGTCGG CCAGGTAGCA TTTGACCATG GTGTCATAGT CCAGCCCCTC CGCGGCGTGG CCCTTGGCGC GCAGCTTGCC CTTGGAGGAG GCGCCGCACG AGGGGCAGTG CAGACTTTTG AGGGCGTAGA GCTTGGGCGC GAGAAATACC GATTCCGGGG AGTAGGCATC CGCGCCGCAG GCCCCGCAGA CGGTCTCGCA TTCCACGAGC CAGGTGAGCT CTGGCCGTTC GGGGTCAAAA ACCAGGTTTC CCCCATGCTT TTTGATGCGT TTCTTACCTC TGGTTTCCAT GAGCCGGTGT CCACGCTCGG TGACGAAAAG GCTGTCCGTG TCCCCGTATA CAGACTTGAG AGGGAGTTTA AACGAATTCA ATAGCTTGTT GCATGGGCGG CGATATAAAA TGCAAGGTGC TGCTCAAAAA ATCAGGCAAA GCCTCGCGCA AAAAAGAAAG CACATCGTAG TCATGCTCAT GCAGATAAAG GCAGGTAAGC TCCGGAACCA CCACAGAAAA AGACACCATT TTTCTCTCAA ACATGTCTGC GGGTTTCTGC ATAAACACAA AATAAAATAA CAAAAAAACA TTTAAACATT AGAAGCCTGT CTTACAACAG GAAAAACAAC CCTTATAAGC ATAAGACGGA CTACGGCCAT GCCGGCGTGA CCGTAAAAAA ACTGGTCACC GTGATTAAAA AGCACCACCG ACAGCTCCTC GGTCATGTCC GGAGTCATAA TGTAAGACTC GGTAAACACA TCAGGTTGAT TCACATCGGT CAGTGCTAAA AAGCGACCGA AATAGCCCGG GGGAATACAT ACCCGCAGGC GTAGAGACAA CATTACAGCC CCCATAGGAG GTATAACAAA ATTAATAGGA GAGAAAAACA CATAAACACC TGAAAAACCC TCCTGCCTAG GCAAAATAGC ACCCTCCCGC TCCAGAACAA CATACAGCGC TTCCACAGCG GCAGCCATAA CAGTCAGCCT TACCAGTAAA AAAGAAAACC TATTAAAAAA ACACCACTCG ACACGGCACC AGCTCAATCA GTCACAGTGT AAAAAAGGGC CAAGTGCAGA GCGAGTATAT ATAGGACTAA AAAATGACGT AACGGTTAAA GTCCACAAAA AACACCCAGA AAACCGCACG CGAACCTACG CCCAGAAACG AAAGCCAAAA AACCCACAAC TTCCTCAAAT CGTCACTTCC GTTTTCCCAC GTTACGTCAC TTCCCATTTT AAGAAAACTA CAATTCCCAA CACATACAAG TTACTCCGCC CTAAAACCTA CGTCACCCGC CCCGTTCCCA CGCCCCGCGC CACGTCACAA ACTCCACCCC CTCATTATCA TATTGGCTTC AATCCAAAAT AAGGTATATT ATTGATGATG TTAATTAACA TGCATGGATC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTGCAGCC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTCA CGTAGAAAGC CAGTCCGCAG AAACGGTGCT GACCCCGGAT GAATGTCAGC TACTGGGCTA TCTGGACAAG GGAAAACGCA AGCGCAAAGA GAAAGCAGGT AGCTTGCAGT GGGCTTACAT GGCGATAGCT AGACTGGGCG GTTTTATGGA CAGCAAGCGA ACCGGAATTG CCAGCTGGGG CGCCCTCTGG TAAGGTTGGG AAGCCCTGCA AAGTAAACTG GATGGCTTTC TTGCCGCCAA GGATCTGATG GCGCAGGGGA TCAAGCTCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAATTTTG TTAAAATTTT TGTTAAATCA
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