pSTOP1622
$128.00
In stock
SKU
PL83058
100μg liquid plasmid(Instantly):$149
| name | pSTOP1622 | another name | apSTOP1622 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | xylR-F：CCTCTGACTTGAGCGTCGAT | |
| 3′ sequencing primer | repB-R：CCTCTGACTTGAGCGTCGAT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTCCGTTA AGATAGTCGA CTCCTATATC AATACCAATC GAGTAGCCTG CATTCTTATT AAAAACAAGC ATTACAGGTC TTCTGCCGCC TCTAGATTGC CCTGCCCCAA TTTCAAAAAT AAAATCTTTT TCAAGCAGTG TATTTACTTG AGAGGAGACA GTAGACTTGT TTAATCCTGT AATCTCAGAG AGAGTTGCCC TGGAGACAGG GGAGTTCTTC AAAATTTCAT CTAATATTAA TTTTTGATTC ATTTTTTTTA CTAAAGCTTG ATCTGCAATT TGAATAATAA CCACTCCTTT GTTTATCCAC CGAACTAAGT TGGTGTTTTT TGAAGCTTGA ATTAGATATT TAAAAGTATC ATATCTAATA TTATAACTAA ATTTTCTAAA AAAAACATTG AAATAAACAT TTATTTTGTA TATGATGAGA TAAAGTTAGT TTATTGGATA AACAAACTAA CTCAATTAAG ATAGTTGATG GATAAACTTG TTCACTTAAA TCAAAGGGGG AAATGTACAA TGGTCCAAAC TAGTTCGAAG ATCTCCGGAG CTCCCGGGAT CCGGTACCGG CCGCATGCCG GCTAAACCTC GCGAACGGAT TCACCGGTCC AAGAATTGGA GCCAATCAAT TCTTGCGGAG AACTGTGAAT GCGCAAACCA ACCCTTGGCA GAACATATCC ATCGCGTCCG CCATCTCCAG CAGCCGCACG CGGCGCATCT CGGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTGCAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAACACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG CCCTTTCGTC TTCAAGAATT CCTGTTATAA AAAAAGGATC AATTTTGAAC TCTCTCCCAA AGTTGATCCC TTAACGATTT AGAAATCCCT TTGAGAATGT TTATATACAT TCAAGGTAAC CAGCCAACTA ATGACAATGA TTCCTGAAAA AAGTAATAAC AAATTACTAT ACAGATAAGT TGACTGATCA ACTTCCATAG GTAACAACCT TTGATCAAGT AAGGGTATGG ATAATAAACC ACCTACAATT GCAATACCTG TTCCCTCTGA TAAAAAGCTG GTAAAGTTAA GCAAACTCAT TCCAGCACCA GCTTCCTGCT GTTTCAAGCT ACTTGAAACA ATTGTTGATA TAACTGTTTT GGTGAACGAA AGCCCACCTA AAACAAATAC GATTATAATT GTCATGAACC ATGATGTTGT TTCTAAAAGA AAGGAAGCAG TTAAAAAGCT AACAGAAAGA AATGTAACTC CGATGTTTAA CACGTATAAA GGACCTCTTC TATCAACAAG TATCCCACCA ATGTAGCCGA AAATAATGAC ACTCATTGTT CCAGGGAAAA TAATTACACT TCCGATTTCG GCAGTACTTA GCTGGTGAAC ATCTTTCATC ATATAAGGAA CCATAGAGAC AAACCCTGCT ACTGTTCCAA ATATAATTCC CCCACAAAGA ACTCCAATCA TAAAAGGTAT ATTTTTCCCT AATCCGGGAT CAACAAAAGG ATCTGTTACT TTCCTGATAT GTTTTACAAA TATCAGGAAT GACAGCACGC TAACGATAAG AAAAGAAATG CTATATGATG TTGTAAACAA CATAAAAAAT ACAATGCCTA CAGACATTAG TATAATTCCT TTGATATCAA AATGACCTTT TATCCTTACT TCTTTCTTTA ATAATTTCAT AAGAAACGGA ACAGTGATAA TTGTTATCAT AGGAATGAGT AGAAGATAGG ACCAATGAAT ATAATGGGCT ATCATTCCAC CAATCGCTGG ACCGACTCCT TCTCCCATGG CTACTATCGA TCCAATAAGA CCAAATGCTT TACCCCTATT TTCCTTTGGA ATATAGCGCG CAACTACAAC CATTACGAGT GCTGGAAATG CAGCTGCACC AGCCCCTTGA ATAAAACGAG CCATAATAAG TAAGGAAAAG AAAGAATGGC CAACAAACCC AATTACCGAC CCGAAACAAT TTATTATAAT TCCAAATAGG AGTAACCTTT TGATGCCTAA TTGATCAGAT AGCTTTCCAT ATACAGCTGT TCCAATGGAA AAGGTTAACA TAAAGGCTGT GTTCACCCAG TTTGTACTCG CAGGTGGTTT ATTAAAATCA TTTGCAATAT CAGGTAATGA GACGTTCAAA ACCATTTCAT TTAATACGCT AAAAAAAGAT AAAATGCAAA GCCAAATTAA AATTTGGTTG TGTCGTAAAT TCGATTGTGA ATAGGATGTA TTCACATTTC ACCCTCCAAT AATGAGGGCA GACGTAGTTT ATAGGGTTAA TGATACGCTT CCCTCTTTTA ATTGAACCCT GTTACATTCA TTACACTTCA TAATTAATTC CTCCTAAACT TGATTAAAAC ATTTTACCAC ATATAAACTA AGTTTTAAAT TCAGTATTTC ATCACTTATA CAACAATATG GCCCGTTTGT TGAACTACTC TTTAATAAAA TAATTTTTCC GTTCCCAATT CCACATTGCA ATAATAGAAA ATCCATCTTC ATCGGCTTTT TCGTCATCAT CTGTATGAAT CAAATCGCCT TCTTCTGTGT CATCAAGGTT TAATTTTTTA TGTATTTCTT TTAACAAACC ACCATAGGAG ATTAACCTTT TACGGTGTAA ACCTTCCTCC AAATCAGACA AACGTTTCAA ATTCTTTTCT TCATCATCGG TCATAAAATC CGTATCCTTT ACAGGATATT TTGCAGTTTC GTCAATTGCC GATTGTATAT CCGATTTATA TTTATTTTTC GGTCGAATCA TTTGAACTTT TACATTTGGA TCATAGTCTA ATTTCATTGC CTTTTTCCAA AATTGAATCC ATTGTTTTTG ATTCACGTAG TTTTCTGTAT TCTTAAAATA AGTTGGTTCC ACACATACCA ATACATGCAT GTGCTGATTA TAAGAATTAT CTTTATTATT TATTGTCACT TCCGTTGCAC GCATAAAACC AACAAGATTT TTATTAATTT TTTTATATTG CATCATTCGG CGAAATCCTT GAGCCATATC TGACAAACTC TTATTTAATT CTTCGCCATC ATAAACATTT TTAACTGTTA ATGTGAGAAA CAACCAACGA ACTGTTGGCT TTTGTTTAAT AACTTCAGCA ACAACCTTTT GTGACTGAAT GCCATGTTTC ATTGCTCTCC TCCAGTTGCA CATTGGACAA AGCCTGGATT TACAAAACCA CACTCGATAC AACTTTCTTT CGCCTGTTTC ACGATTTTGT TTATACTCTA ATATTTCAGC ACAATCTTTT ACTCTTTCAG CCTTTTTAAA TTCAAGAATA TGCAGAAGTT CAAAGTAATC AACATTAGCG ATTTTCTTTT CTCTCCATGG TCTCACTTTT CCACTTTTTG TCTTGTCCAC TAAAACCCTT GATTTTTCAT CTGAATAAAT GCTACTATTA GGACACATAA TATTAAAAGA AACCCCCATC TATTTAGTTA TTTGTTTGGT CACTTATAAC TTTAACAGAT GGGGTTTTTC TGTGCAACCA ATTTTAAGGG TTTTCAATAC TTTAAAACAC ATACATACCA ACACTTCAAC GCACCTTTCA GCAACTAAAA TAAAAATGAC GTTATTTCTA TATGTATCAA GATAAGAAAG AACAAGTTCA AAACCATCAA AAAAAGACAC CTTTTCAGGT GCTTTTTTTA TTTTATAAAC TCATTCCCTG ATCTCGACTT CGTTCTTTTT TTACCTCTCG GTTATGAGTT AGTTCAAATT CGTTCTTTTT AGGTTCTAAA TCGTGTTTTT CTTGGAATTG TGCTGTTTTA TCCTTTACCT TGTCTACAAA CCCCTTAAAA ACGTTTTTAA AGGCTTTTAA GCCGTCTGTA CGTTCCTTAA GATCAACGTG ATATAGGTTT GCTAACCTTT GCGTTCACTT AACTAACTTA TAGGGGTAAC ACTTAAAAAA GAATCAATAA CGATAGAAAC CGCTCCTAAA GCAGGTGCAT TTTTTCCTAA CGAAGAAGGC AATAGTTCAC ATTTATTGTC TAAATGAGAA TGGACTCTAG AAGAAACTTC GTTTTTAATC GTATTTAAAA CAATGGGATG AGATTCAATT ATATGATTTC TCAAGATAAC AGCTTCTATA TCAAATGTAT TAAGGATATT GGTTAATCCA ATTCCGATAT AAAAGCCAAA GTTTTGAAGT GCATTTAACA TTTCTACATC ATTTTTATTT GCGCGTTCCA CAATCTCTTT TCGAGAAATA TTCTTTTCTT CTTTAGAGAG CGAAGCCAGT AACGCTTTTT CAGAAGCATA TAATTCCCAA CAGCCTCGAT TTCCACAGCT GCATTTGGGT CCATTAAAAT CTATCGTCAT ATGACCCATT TCCCCAGAAA AACCCTGAAC ACCTTTATAC AATTCGTTGT TAATAACAAG TCCAGTTCCA ATTCCGATAT TAATACTGAT GTAAACGATG TTTTCATAGT TTTTTGTCAT ACCAAATACT TTTTCACCGT ATGCTCCTGC ATTAGCTTCA TTTTCAACAA AAACCGGAAC ATTAAACTCA CTCTCAATTA AAAACTGCAA ATCTTTGATA TTCCAATTTA AGTTAGGCAT GAAAATAATT TGCTGATGAC GATCTACAAG GCCTGGAACA CAAATTCCTA TTCCGACTAG ACCATAAGGG GACTCAGGCA TATGGGTTAC AAAACCATGA ATAAGTGCAA ATAAAATCTC TTTTACTTCA CTAGCGGAAG AACTAGACAA GTCAGAAGTC TTCTCGAGAA TAATATTTCC TTCTAAGTCG GTTAG
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