pCAMBIA1380
$128.00
In stock
SKU
PL83053
100μg liquid plasmid(Instantly):$163
| name | pCAMBIA1380 | another name | apCAMBIA1380 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTAGCCACCA CCACCACCAC CACGTGTGAA TTACAGGTGA CCAGCTCGAA TTTCCCCGAT CGTTCAAACA TTTGGCAATA AAGTTTCTTA AGATTGAATC CTGTTGCCGG TCTTGCGATG ATTATCATAT AATTTCTGTT GAATTACGTT AAGCATGTAA TAATTAACAT GTAATGCATG ACGTTATTTA TGAGATGGGT TTTTATGATT AGAGTCCCGC AATTATACAT TTAATACGCG ATAGAAAACA AAATATAGCG CGCAAACTAG GATAAATTAT CGCGCGCGGT GTCATCTATG TTACTAGATC GGGAATTAAA CTATCAGTGT TTGACAGGAT ATATTGGCGG GTAAACCTAA GAGAAAAGAG CGTTTATTAG AATAACGGAT ATTTAAAAGG GCGTGAAAAG GTTTATCCGT TCGTCCATTT GTATGTGCAT GCCAACCACA GGGTTCCCCT CGGGATCAAA GTACTTTGAT CCAACCCCTC CGCTGCTATA GTGCAGTCGG CTTCTGACGT TCAGTGCAGC CGTCTTCTGA AAACGACATG TCGCACAAGT CCTAAGTTAC GCGACAGGCT GCCGCCCTGC CCTTTTCCTG GCGTTTTCTT GTCGCGTGTT TTAGTCGCAT AAAGTAGAAT ACTTGCGACT AGAACCGGAG ACATTACGCC ATGAACAAGA GCGCCGCCGC TGGCCTGCTG GGCTATGCCC GCGTCAGCAC CGACGACCAG GACTTGACCA ACCAACGGGC CGAACTGCAC GCGGCCGGCT GCACCAAGCT GTTTTCCGAG AAGATCACCG GCACCAGGCG CGACCGCCCG GAGCTGGCCA GGATGCTTGA CCACCTACGC CCTGGCGACG TTGTGACAGT GACCAGGCTA GACCGCCTGG CCCGCAGCAC CCGCGACCTA CTGGACATTG CCGAGCGCAT CCAGGAGGCC GGCGCGGGCC TGCGTAGCCT GGCAGAGCCG TGGGCCGACA CCACCACGCC GGCCGGCCGC ATGGTGTTGA CCGTGTTCGC CGGCATTGCC GAGTTCGAGC GTTCCCTAAT CATCGACCGC ACCCGGAGCG GGCGCGAGGC CGCCAAGGCC CGAGGCGTGA AGTTTGGCCC CCGCCCTACC CTCACCCCGG CACAGATCGC GCACGCCCGC GAGCTGATCG ACCAGGAAGG CCGCACCGTG AAAGAGGCGG CTGCACTGCT TGGCGTGCAT CGCTCGACCC TGTACCGCGC ACTTGAGCGC AGCGAGGAAG TGACGCCCAC CGAGGCCAGG CGGCGCGGTG CCTTCCGTGA GGACGCATTG ACCGAGGCCG ACGCCCTGGC GGCCGCCGAG AATGAACGCC AAGAGGAACA AGCATGAAAC CGCACCAGGA CGGCCAGGAC GAACCGTTTT TCATTACCGA AGAGATCGAG GCGGAGATGA TCGCGGCCGG GTACGTGTTC GAGCCGCCCG CGCACGTCTC AACCGTGCGG CTGCATGAAA TCCTGGCCGG TTTGTCTGAT GCCAAGCTGG CGGCCTGGCC GGCCAGCTTG GCCGCTGAAG AAACCGAGCG CCGCCGTCTA AAAAGGTGAT GTGTATTTGA GTAAAACAGC TTGCGTCATG CGGTCGCTGC GTATATGATG CGATGAGTAA ATAAACAAAT ACGCAAGGGG AACGCATGAA GGTTATCGCT GTACTTAACC AGAAAGGCGG GTCAGGCAAG ACGACCATCG CAACCCATCT AGCCCGCGCC CTGCAACTCG CCGGGGCCGA TGTTCTGTTA GTCGATTCCG ATCCCCAGGG CAGTGCCCGC GATTGGGCGG CCGTGCGGGA AGATCAACCG CTAACCGTTG TCGGCATCGA CCGCCCGACG ATTGACCGCG ACGTGAAGGC CATCGGCCGG CGCGACTTCG TAGTGATCGA CGGAGCGCCC CAGGCGGCGG ACTTGGCTGT GTCCGCGATC AAGGCAGCCG ACTTCGTGCT GATTCCGGTG CAGCCAAGCC CTTACGACAT ATGGGCCACC GCCGACCTGG TGGAGCTGGT TAAGCAGCGC ATTGAGGTCA CGGATGGAAG GCTACAAGCG GCCTTTGTCG TGTCGCGGGC GATCAAAGGC ACGCGCATCG GCGGTGAGGT TGCCGAGGCG CTGGCCGGGT ACGAGCTGCC CATTCTTGAG TCCCGTATCA CGCAGCGCGT GAGCTACCCA GGCACTGCCG CCGCCGGCAC AACCGTTCTT GAATCAGAAC CCGAGGGCGA CGCTGCCCGC GAGGTCCAGG CGCTGGCCGC TGAAATTAAA TCAAAACTCA TTTGAGTTAA TGAGGTAAAG AGAAAATGAG CAAAAGCACA AACACGCTAA GTGCCGGCCG TCCGAGCGCA CGCAGCAGCA AGGCTGCAAC GTTGGCCAGC CTGGCAGACA CGCCAGCCAT GAAGCGGGTC AACTTTCAGT TGCCGGCGGA GGATCACACC AAGCTGAAGA TGTACGCGGT ACGCCAAGGC AAGACCATTA CCGAGCTGCT ATCTGAATAC ATCGCGCAGC TACCAGAGTA AATGAGCAAA TGAATAAATG AGTAGATGAA TTTTAGCGGC TAAAGGAGGC GGCATGGAAA ATCAAGAACA ACCAGGCACC GACGCCGTGG AATGCCCCAT GTGTGGAGGA ACGGGCGGTT GGCCAGGCGT AAGCGGCTGG GTTGTCTGCC GGCCCTGCAA TGGCACTGGA ACCCCCAAGC CCGAGGAATC GGCGTGACGG TCGCAAACCA TCCGGCCCGG TACAAATCGG CGCGGCGCTG GGTGATGACC TGGTGGAGAA GTTGAAGGCC GCGCAGGCCG CCCAGCGGCA ACGCATCGAG GCAGAAGCAC GCCCCGGTGA ATCGTGGCAA GCGGCCGCTG ATCGAATCCG CAAAGAATCC CGGCAACCGC CGGCAGCCGG TGCGCCGTCG ATTAGGAAGC CGCCCAAGGG CGACGAGCAA CCAGATTTTT TCGTTCCGAT GCTCTATGAC GTGGGCACCC GCGATAGTCG CAGCATCATG GACGTGGCCG TTTTCCGTCT GTCGAAGCGT GACCGACGAG CTGGCGAGGT GATCCGCTAC GAGCTTCCAG ACGGGCACGT AGAGGTTTCC GCAGGGCCGG CCGGCATGGC CAGTGTGTGG GATTACGACC TGGTACTGAT GGCGGTTTCC CATCTAACCG AATCCATGAA CCGATACCGG GAAGGGAAGG GAGACAAGCC CGGCCGCGTG TTCCGTCCAC ACGTTGCGGA CGTACTCAAG TTCTGCCGGC GAGCCGATGG CGGAAAGCAG AAAGACGACC TGGTAGAAAC CTGCATTCGG TTAAACACCA CGCACGTTGC CATGCAGCGT ACGAAGAAGG CCAAGAACGG CCGCCTGGTG ACGGTATCCG AGGGTGAAGC CTTGATTAGC CGCTACAAGA TCGTAAAGAG CGAAACCGGG CGGCCGGAGT ACATCGAGAT CGAGCTAGCT GATTGGATGT ACCGCGAGAT CACAGAAGGC AAGAACCCGG ACGTGCTGAC GGTTCACCCC GATTACTTTT TGATCGATCC CGGCATCGGC CGTTTTCTCT ACCGCCTGGC ACGCCGCGCC GCAGGCAAGG CAGAAGCCAG ATGGTTGTTC AAGACGATCT ACGAACGCAG TGGCAGCGCC GGAGAGTTCA AGAAGTTCTG TTTCACCGTG CGCAAGCTGA TCGGGTCAAA TGACCTGCCG GAGTACGATT TGAAGGAGGA GGCGGGGCAG GCTGGCCCGA TCCTAGTCAT GCGCTACCGC AACCTGATCG AGGGCGAAGC ATCCGCCGGT TCCTAATGTA CGGAGCAGAT GCTAGGGCAA ATTGCCCTAG CAGGGGAAAA AGGTCGAAAA GGTCTCTTTC CTGTGGATAG CACGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG AACCCGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGTCACACAT GTAAGTGACT GATATAAAAG AGAAAAAAGG CGATTTTTCC GCCTAAAACT CTTTAAAACT TATTAAAACT CTTAAAACCC GCCTGGCCTG TGCATAACTG TCTGGCCAGC GCACAGCCGA AGAGCTGCAA AAAGCGCCTA CCCTTCGGTC GCTGCGCTCC CTACGCCCCG CCGCTTCGCG TCGGCCTATC GCGGCCGCTG GCCGCTCAAA AATGGCTGGC CTACGGCCAG GCAATCTACC AGGGCGCGGA CAAGCCGCGC CGTCGCCACT CGACCGCCGG CGCCCACATC AAGGCACCCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC ATTCTAGGTA CTAAAACAAT TCATCCAGTA AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA AAAAATAGCT CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG GCAATGTCAT ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT TTGCCATCTT TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC TCTTCGGGCT TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG TCTTCTTCCC AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT TCGGCTAAGC GGCTGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA AAGAGCCTGA TGCACTCCGC ATACAGCTCG ATAATCTTTT CAGGGCTTTG TTCATCTTCA TACTCTTCCG AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA TGCCGTTCAA AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG TCCTTTTCCC GTTCCACATC ATAGGTGGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA TATAGGTTTT CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA TCTTTTACGC AGCGGTATTT TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA GCCATTTATT ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT ATAACAAGAC GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACCAGAAAA CAGCTTTTTC AAAGTTGTTT TCAAAGTTGG CGTATAACAT AGTATCGACG GAGCCGATTT TGAAACCGCG GTGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC TCCGCGAGAT CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT CGGTAACATG AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGGT GGCAGGATAT ATTGTGGTGT AAACAAATTG ACGCTTAGAC AACTTAATAA CACATTGCGG ACGTTTTTAA TGTACTGAAT TAACGCCGAA TTAATTCGGG GGATCTGGAT TTTAGTACTG GATTTTGGTT TTAGGAATTA GAAATTTTAT TGATAGAAGT ATTTTACAAA TACAAATACA TACTAAGGGT TTCTTATATG CTCAACACAT GAGCGAAACC CTATAGGAAC CCTAATTCCC TTATCTGGGA ACTACTCACA CATTATTATG GAGAAACTCG AGCTTGTCGA TCGACAGATC CGGTCGGCAT CTACTCTATT TCTTTGCCCT CGGACGAGTG CTGGGGCGTC GGTTTCCACT ATCGGCGAGT ACTTCTACAC AGCCATCGGT CCAGACGGCC GCGCTTCTGC GGGCGATTTG TGTACGCCCG ACAGTCCCGG CTCCGGATCG GACGATTGCG TCGCATCGAC CCTGCGCCCA AGCTGCATCA TCGAAATTGC CGTCAACCAA GCTCTGATAG AGTTGGTCAA GACCAATGCG GAGCATATAC GCCCGGAGTC GTGGCGATCC TGCAAGCTCC GGATGCCTCC GCTCGAAGTA GCGCGTCTGC TGCTCCATAC AAGCCAACCA CGGCCTCCAG AAGAAGATGT TGGCGACCTC GTATTGGGAA TCCCCGAACA TCGCCTCGCT CCAGTCAATG ACCGCTGTTA TGCGGCCATT GTCCGTCAGG ACATTGTTGG AGCCGAAATC CGCGTGCACG AGGTGCCGGA CTTCGGGGCA GTCCTCGGCC CAAAGCATCA GCTCATCGAG AGCCTGCGCG ACGGACGCAC TGACGGTGTC GTCCATCACA GTTTGCCAGT GATACACATG GGGATCAGCA ATCGCGCATA TGAAATCACG CCATGTAGTG TATTGACCGA TTCCTTGCGG TCCGAATGGG CCGAACCCGC TCGTCTGGCT AAGATCGGCC GCAGCGATCG CATCCATAGC CTCCGCGACC GGTTGTAGAA CAGCGGGCAG TTCGGTTTCA GGCAGGTCTT GCAACGTGAC ACCCTGTGCA CGGCGGGAGA TGCAATAGGT CAGGCTCTCG CTAAACTCCC CAATGTCAAG CACTTCCGGA ATCGGGAGCG CGGCCGATGC AAAGTGCCGA TAAACATAAC GATCTTTGTA GAAACCATCG GCGCAGCTAT TTACCCGCAG GACATATCCA CGCCCTCCTA CATCGAAGCT GAAAGCACGA GATTCTTCGC CCTCCGAGAG CTGCATCAGG TCGGAGACGC TGTCGAACTT TTCGATCAGA AACTTCTCGA CAGACGTCGC GGTGAGTTCA GGCTTTTTCA TATCTCATTG CCCCCCGGGA TCTGCGAAAG CTCGAGAGAG ATAGATTTGT AGAGAGAGAC TGGTGATTTC AGCGTGTCCT CTCCAAATGA AATGAACTTC CTTATATAGA GGAAGGTCTT GCGAAGGATA GTGGGATTGT GCGTCATCCC TTACGTCAGT GGAGATATCA CATCAATCCA CTTGCTTTGA AGACGTGGTT GGAACGTCTT CTTTTTCCAC GATGCTCCTC GTGGGTGGGG GTCCATCTTT GGGACCACTG TCGGCAGAGG CATCTTGAAC GATAGCCTTT CCTTTATCGC AATGATGGCA TTTGTAGGTG CCACCTTCCT TTTCTACTGT CCTTTTGATG AAGTGACAGA TAGCTGGGCA ATGGAATCCG AGGAGGTTTC CCGATATTAC CCTTTGTTGA AAAGTCTCAA TAGCCCTTTG GTCTTCTGAG ACTGTATCTT TGATATTCTT GGAGTAGACG AGAGTGTCGT GCTCCACCAT GTTATCACAT CAATCCACTT GCTTTGAAGA CGTGGTTGGA ACGTCTTCTT TTTCCACGAT GCTCCTCGTG GGTGGGGGTC CATCTTTGGG ACCACTGTCG GCAGAGGCAT CTTGAACGAT AGCCTTTCCT TTATCGCAAT GATGGCATTT GTAGGTGCCA CCTTCCTTTT CTACTGTCCT TTTGATGAAG TGACAGATAG CTGGGCAATG GAATCCGAGG AGGTTTCCCG ATATTACCCT TTGTTGAAAA GTCTCAATAG CCCTTTGGTC TTCTGAGACT GTATCTTTGA TATTCTTGGA GTAGACGAGA GTGTCGTGCT CCACCATGTT GGGCCCGGCG CGCCGAATTC CCGGGGATCC GTCGACCTGC AGCCAAGCTT TAAGAGGAGT CCACCATGGT AGATCTGACT AGTGTTAACG
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