pCAMBIA3301
$157.00
In stock
SKU
PL83028
100μg liquid plasmid(Instantly):$192
| name | pCAMBIA3301 | another name | apCAMBIA3301 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CATGGTAGAT CTGAGGGTAA ATTTCTAGTT TTTCTCCTTC ATTTTCTTGG TTAGGACCCT TTTCTCTTTT TATTTTTTTG AGCTTTGATC TTTCTTTAAA CTGATCTATT TTTTAATTGA TTGGTTATGG TGTAAATATT ACATAGCTTT AACTGATAAT CTGATTACTT TATTTCGTGT GTCTATGATG ATGATGATAG TTACAGAACC GACGACTCGT CCGTCCTGTA GAAACCCCAA CCCGTGAAAT CAAAAAACTC GACGGCCTGT GGGCATTCAG TCTGGATCGC GAAAACTGTG GAATTGATCA GCGTTGGTGG GAAAGCGCGT TACAAGAAAG CCGGGCAATT GCTGTGCCAG GCAGTTTTAA CGATCAGTTC GCCGATGCAG ATATTCGTAA TTATGCGGGC AACGTCTGGT ATCAGCGCGA AGTCTTTATA CCGAAAGGTT GGGCAGGCCA GCGTATCGTG CTGCGTTTCG ATGCGGTCAC TCATTACGGC AAAGTGTGGG TCAATAATCA GGAAGTGATG GAGCATCAGG GCGGCTATAC GCCATTTGAA GCCGATGTCA CGCCGTATGT TATTGCCGGG AAAAGTGTAC GTATCACCGT TTGTGTGAAC AACGAACTGA ACTGGCAGAC TATCCCGCCG GGAATGGTGA TTACCGACGA AAACGGCAAG AAAAAGCAGT CTTACTTCCA TGATTTCTTT AACTATGCCG GAATCCATCG CAGCGTAATG CTCTACACCA CGCCGAACAC CTGGGTGGAC GATATCACCG TGGTGACGCA TGTCGCGCAA GACTGTAACC ACGCGTCTGT TGACTGGCAG GTGGTGGCCA ATGGTGATGT CAGCGTTGAA CTGCGTGATG CGGATCAACA GGTGGTTGCA ACTGGACAAG GCACTAGCGG GACTTTGCAA GTGGTGAATC CGCACCTCTG GCAACCGGGT GAAGGTTATC TCTATGAACT CGAAGTCACA GCCAAAAGCC AGACAGAGTC TGATATCTAC CCGCTTCGCG TCGGCATCCG GTCAGTGGCA GTGAAGGGCC AACAGTTCCT GATTAACCAC AAACCGTTCT ACTTTACTGG CTTTGGTCGT CATGAAGATG CGGACTTACG TGGCAAAGGA TTCGATAACG TGCTGATGGT GCACGACCAC GCATTAATGG ACTGGATTGG GGCCAACTCC TACCGTACCT CGCATTACCC TTACGCTGAA GAGATGCTCG ACTGGGCAGA TGAACATGGC ATCGTGGTGA TTGATGAAAC TGCTGCTGTC GGCTTTCAGC TGTCTTTAGG CATTGGTTTC GAAGCGGGCA ACAAGCCGAA AGAACTGTAC AGCGAAGAGG CAGTCAACGG GGAAACTCAG CAAGCGCACT TACAGGCGAT TAAAGAGCTG ATAGCGCGTG ACAAAAACCA CCCAAGCGTG GTGATGTGGA GTATTGCCAA CGAACCGGAT ACCCGTCCGC AAGGTGCACG GGAATATTTC GCGCCACTGG CGGAAGCAAC GCGTAAACTC GACCCGACGC GTCCGATCAC CTGCGTCAAT GTAATGTTCT GCGACGCTCA CACCGATACC ATCAGCGATC TCTTTGATGT GCTGTGCCTG AACCGTTATT ACGGATGGTA TGTCCAAAGC GGCGATTTGG AAACGGCAGA GAAGGTACTG GAAAAAGAAC TTCTGGCCTG GCAGGAGAAA CTGCATCAGC CGATTATCAT CACCGAATAC GGCGTGGATA CGTTAGCCGG GCTGCACTCA ATGTACACCG ACATGTGGAG TGAAGAGTAT CAGTGTGCAT GGCTGGATAT GTATCACCGC GTCTTTGATC GCGTCAGCGC CGTCGTCGGT GAACAGGTAT GGAATTTCGC CGATTTTGCG ACCTCGCAAG GCATATTGCG CGTTGGCGGT AACAAGAAAG GGATCTTCAC TCGCGACCGC AAACCGAAGT CGGCGGCTTT TCTGCTGCAA AAACGCTGGA CTGGCATGAA CTTCGGTGAA AAACCGCAGC AGGGAGGCAA ACAAGCTAGC CACCACCACC ACCACCACGT GTGAATTACA GGTGACCAGC TCGAATTTCC CCGATCGTTC AAACATTTGG CAATAAAGTT TCTTAAGATT GAATCCTGTT GCCGGTCTTG CGATGATTAT CATATAATTT CTGTTGAATT ACGTTAAGCA TGTAATAATT AACATGTAAT GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA ATTATCGCGC GCGGTGTCAT CTATGTTACT AGATCGGGAA TTAAACTATC AGTGTTTGAC AGGATATATT GGCGGGTAAA CCTAAGAGAA AAGAGCGTTT ATTAGAATAA CGGATATTTA AAAGGGCGTG AAAAGGTTTA TCCGTTCGTC CATTTGTATG TGCATGCCAA CCACAGGGTT CCCCTCGGGA TCAAAGTACT TTGATCCAAC CCCTCCGCTG CTATAGTGCA GTCGGCTTCT GACGTTCAGT GCAGCCGTCT TCTGAAAACG ACATGTCGCA CAAGTCCTAA GTTACGCGAC AGGCTGCCGC CCTGCCCTTT TCCTGGCGTT TTCTTGTCGC GTGTTTTAGT CGCATAAAGT AGAATACTTG CGACTAGAAC CGGAGACATT ACGCCATGAA CAAGAGCGCC GCCGCTGGCC TGCTGGGCTA TGCCCGCGTC AGCACCGACG ACCAGGACTT GACCAACCAA CGGGCCGAAC TGCACGCGGC CGGCTGCACC AAGCTGTTTT CCGAGAAGAT CACCGGCACC AGGCGCGACC GCCCGGAGCT GGCCAGGATG CTTGACCACC TACGCCCTGG CGACGTTGTG ACAGTGACCA GGCTAGACCG CCTGGCCCGC AGCACCCGCG ACCTACTGGA CATTGCCGAG CGCATCCAGG AGGCCGGCGC GGGCCTGCGT AGCCTGGCAG AGCCGTGGGC CGACACCACC ACGCCGGCCG GCCGCATGGT GTTGACCGTG TTCGCCGGCA TTGCCGAGTT CGAGCGTTCC CTAATCATCG ACCGCACCCG GAGCGGGCGC GAGGCCGCCA AGGCCCGAGG CGTGAAGTTT GGCCCCCGCC CTACCCTCAC CCCGGCACAG ATCGCGCACG CCCGCGAGCT GATCGACCAG GAAGGCCGCA CCGTGAAAGA GGCGGCTGCA CTGCTTGGCG TGCATCGCTC GACCCTGTAC CGCGCACTTG AGCGCAGCGA GGAAGTGACG CCCACCGAGG CCAGGCGGCG CGGTGCCTTC CGTGAGGACG CATTGACCGA GGCCGACGCC CTGGCGGCCG CCGAGAATGA ACGCCAAGAG GAACAAGCAT GAAACCGCAC CAGGACGGCC AGGACGAACC GTTTTTCATT ACCGAAGAGA TCGAGGCGGA GATGATCGCG GCCGGGTACG TGTTCGAGCC GCCCGCGCAC GTCTCAACCG TGCGGCTGCA TGAAATCCTG GCCGGTTTGT CTGATGCCAA GCTGGCGGCC TGGCCGGCCA GCTTGGCCGC TGAAGAAACC GAGCGCCGCC GTCTAAAAAG GTGATGTGTA TTTGAGTAAA ACAGCTTGCG TCATGCGGTC GCTGCGTATA TGATGCGATG AGTAAATAAA CAAATACGCA AGGGGAACGC ATGAAGGTTA TCGCTGTACT TAACCAGAAA GGCGGGTCAG GCAAGACGAC CATCGCAACC CATCTAGCCC GCGCCCTGCA ACTCGCCGGG GCCGATGTTC TGTTAGTCGA TTCCGATCCC CAGGGCAGTG CCCGCGATTG GGCGGCCGTG CGGGAAGATC AACCGCTAAC CGTTGTCGGC ATCGACCGCC CGACGATTGA CCGCGACGTG AAGGCCATCG GCCGGCGCGA CTTCGTAGTG ATCGACGGAG CGCCCCAGGC GGCGGACTTG GCTGTGTCCG CGATCAAGGC AGCCGACTTC GTGCTGATTC CGGTGCAGCC AAGCCCTTAC GACATATGGG CCACCGCCGA CCTGGTGGAG CTGGTTAAGC AGCGCATTGA GGTCACGGAT GGAAGGCTAC AAGCGGCCTT TGTCGTGTCG CGGGCGATCA AAGGCACGCG CATCGGCGGT GAGGTTGCCG AGGCGCTGGC CGGGTACGAG CTGCCCATTC TTGAGTCCCG TATCACGCAG CGCGTGAGCT ACCCAGGCAC TGCCGCCGCC GGCACAACCG TTCTTGAATC AGAACCCGAG GGCGACGCTG CCCGCGAGGT CCAGGCGCTG GCCGCTGAAA TTAAATCAAA ACTCATTTGA GTTAATGAGG TAAAGAGAAA ATGAGCAAAA GCACAAACAC GCTAAGTGCC GGCCGTCCGA GCGCACGCAG CAGCAAGGCT GCAACGTTGG CCAGCCTGGC AGACACGCCA GCCATGAAGC GGGTCAACTT TCAGTTGCCG GCGGAGGATC ACACCAAGCT GAAGATGTAC GCGGTACGCC AAGGCAAGAC CATTACCGAG CTGCTATCTG AATACATCGC GCAGCTACCA GAGTAAATGA GCAAATGAAT AAATGAGTAG ATGAATTTTA GCGGCTAAAG GAGGCGGCAT GGAAAATCAA GAACAACCAG GCACCGACGC CGTGGAATGC CCCATGTGTG GAGGAACGGG CGGTTGGCCA GGCGTAAGCG GCTGGGTTGT CTGCCGGCCC TGCAATGGCA CTGGAACCCC CAAGCCCGAG GAATCGGCGT GACGGTCGCA AACCATCCGG CCCGGTACAA ATCGGCGCGG CGCTGGGTGA TGACCTGGTG GAGAAGTTGA AGGCCGCGCA GGCCGCCCAG CGGCAACGCA TCGAGGCAGA AGCACGCCCC GGTGAATCGT GGCAAGCGGC CGCTGATCGA ATCCGCAAAG AATCCCGGCA ACCGCCGGCA GCCGGTGCGC CGTCGATTAG GAAGCCGCCC AAGGGCGACG AGCAACCAGA TTTTTTCGTT CCGATGCTCT ATGACGTGGG CACCCGCGAT AGTCGCAGCA TCATGGACGT GGCCGTTTTC CGTCTGTCGA AGCGTGACCG ACGAGCTGGC GAGGTGATCC GCTACGAGCT TCCAGACGGG CACGTAGAGG TTTCCGCAGG GCCGGCCGGC ATGGCCAGTG TGTGGGATTA CGACCTGGTA CTGATGGCGG TTTCCCATCT AACCGAATCC ATGAACCGAT ACCGGGAAGG GAAGGGAGAC AAGCCCGGCC GCGTGTTCCG TCCACACGTT GCGGACGTAC TCAAGTTCTG CCGGCGAGCC GATGGCGGAA AGCAGAAAGA CGACCTGGTA GAAACCTGCA TTCGGTTAAA CACCACGCAC GTTGCCATGC AGCGTACGAA GAAGGCCAAG AACGGCCGCC TGGTGACGGT ATCCGAGGGT GAAGCCTTGA TTAGCCGCTA CAAGATCGTA AAGAGCGAAA CCGGGCGGCC GGAGTACATC GAGATCGAGC TAGCTGATTG GATGTACCGC GAGATCACAG AAGGCAAGAA CCCGGACGTG CTGACGGTTC ACCCCGATTA CTTTTTGATC GATCCCGGCA TCGGCCGTTT TCTCTACCGC CTGGCACGCC GCGCCGCAGG CAAGGCAGAA GCCAGATGGT TGTTCAAGAC GATCTACGAA CGCAGTGGCA GCGCCGGAGA GTTCAAGAAG TTCTGTTTCA CCGTGCGCAA GCTGATCGGG TCAAATGACC TGCCGGAGTA CGATTTGAAG GAGGAGGCGG GGCAGGCTGG CCCGATCCTA GTCATGCGCT ACCGCAACCT GATCGAGGGC GAAGCATCCG CCGGTTCCTA ATGTACGGAG CAGATGCTAG GGCAAATTGC CCTAGCAGGG GAAAAAGGTC GAAAAGGTCT CTTTCCTGTG GATAGCACGT ACATTGGGAA CCCAAAGCCG TACATTGGGA ACCGGAACCC GTACATTGGG AACCCAAAGC CGTACATTGG GAACCGGTCA CACATGTAAG TGACTGATAT AAAAGAGAAA AAAGGCGATT TTTCCGCCTA AAACTCTTTA AAACTTATTA AAACTCTTAA AACCCGCCTG GCCTGTGCAT AACTGTCTGG CCAGCGCACA GCCGAAGAGC TGCAAAAAGC GCCTACCCTT CGGTCGCTGC GCTCCCTACG CCCCGCCGCT TCGCGTCGGC CTATCGCGGC CGCTGGCCGC TCAAAAATGG CTGGCCTACG GCCAGGCAAT CTACCAGGGC GCGGACAAGC CGCGCCGTCG CCACTCGACC GCCGGCGCCC ACATCAAGGC ACCCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCGCAGC CATGACCCAG TCACGTAGCG ATAGCGGAGT GTATACTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGCATTCT AGGTACTAAA ACAATTCATC CAGTAAAATA TAATATTTTA TTTTCTCCCA ATCAGGCTTG ATCCCCAGTA AGTCAAAAAA TAGCTCGACA TACTGTTCTT CCCCGATATC CTCCCTGATC GACCGGACGC AGAAGGCAAT GTCATACCAC TTGTCCGCCC TGCCGCTTCT CCCAAGATCA ATAAAGCCAC TTACTTTGCC ATCTTTCACA AAGATGTTGC TGTCTCCCAG GTCGCCGTGG GAAAAGACAA GTTCCTCTTC GGGCTTTTCC GTCTTTAAAA AATCATACAG CTCGCGCGGA TCTTTAAATG GAGTGTCTTC TTCCCAGTTT TCGCAATCCA CATCGGCCAG ATCGTTATTC AGTAAGTAAT CCAATTCGGC TAAGCGGCTG TCTAAGCTAT TCGTATAGGG ACAATCCGAT ATGTCGATGG AGTGAAAGAG CCTGATGCAC TCCGCATACA GCTCGATAAT CTTTTCAGGG CTTTGTTCAT CTTCATACTC TTCCGAGCAA AGGACGCCAT CGGCCTCACT CATGAGCAGA TTGCTCCAGC CATCATGCCG TTCAAAGTGC AGGACCTTTG GAACAGGCAG CTTTCCTTCC AGCCATAGCA TCATGTCCTT TTCCCGTTCC ACATCATAGG TGGTCCCTTT ATACCGGCTG TCCGTCATTT TTAAATATAG GTTTTCATTT TCTCCCACCA GCTTATATAC CTTAGCAGGA GACATTCCTT CCGTATCTTT TACGCAGCGG TATTTTTCGA TCAGTTTTTT CAATTCCGGT GATATTCTCA TTTTAGCCAT TTATTATTTC CTTCCTCTTT TCTACAGTAT TTAAAGATAC CCCAAGAAGC TAATTATAAC AAGACGAACT CCAATTCACT GTTCCTTGCA TTCTAAAACC TTAAATACCA GAAAACAGCT TTTTCAAAGT TGTTTTCAAA GTTGGCGTAT AACATAGTAT CGACGGAGCC GATTTTGAAA CCGCGGTGAT CACAGGCAGC AACGCTCTGT CATCGTTACA ATCAACATGC TACCCTCCGC GAGATCATCC GTGTTTCAAA CCCGGCAGCT TAGTTGCCGT TCTTCCGAAT AGCATCGGTA ACATGAGCAA AGTCTGCCGC CTTACAACGG CTCTCCCGCT GACGCCGTCC CGGACTGATG GGCTGCCTGT ATCGAGTGGT GATTTTGTGC CGAGCTGCCG GTCGGGGAGC TGTTGGCTGG CTGGTGGCAG GATATATTGT GGTGTAAACA AATTGACGCT TAGACAACTT AATAACACAT TGCGGACGTT TTTAATGTAC TGAATTAACG CCGAATTAAT TCGGGGGATC TGGATTTTAG TACTGGATTT TGGTTTTAGG AATTAGAAAT TTTATTGATA GAAGTATTTT ACAAATACAA ATACATACTA AGGGTTTCTT ATATGCTCAA CACATGAGCG AAACCCTATA GGAACCCTAA TTCCCTTATC TGGGAACTAC TCACACATTA TTATGGAGAA ACTCGAGTCA AATCTCGGTG ACGGGCAGGA CCGGACGGGG CGGTACCGGC AGGCTGAAGT CCAGCTGCCA GAAACCCACG TCATGCCAGT TCCCGTGCTT GAAGCCGGCC GCCCGCAGCA TGCCGCGGGG GGCATATCCG AGCGCCTCGT GCATGCGCAC GCTCGGGTCG TTGGGCAGCC CGATGACAGC GACCACGCTC TTGAAGCCCT GTGCCTCCAG GGACTTCAGC AGGTGGGTGT AGAGCGTGGA GCCCAGTCCC GTCCGCTGGT GGCGGGGGGA GACGTACACG GTCGACTCGG CCGTCCAGTC GTAGGCGTTG CGTGCCTTCC AGGGGCCCGC GTAGGCGATG CCGGCGACCT CGCCGTCCAC CTCGGCGACG AGCCAGGGAT AGCGCTCCCG CAGACGGACG AGGTCGTCCG TCCACTCCTG CGGTTCCTGC GGCTCGGTAC GGAAGTTGAC CGTGCTTGTC TCGATGTAGT GGTTGACGAT GGTGCAGACC GCCGGCATGT CCGCCTCGGT GGCACGGCGG ATGTCGGCCG GGCGTCGTTC TGGGCTCATG GTAGACTCGA GAGAGATAGA TTTGTAGAGA GAGACTGGTG ATTTCAGCGT GTCCTCTCCA AATGAAATGA ACTTCCTTAT ATAGAGGAAG GTCTTGCGAA GGATAGTGGG ATTGTGCGTC ATCCCTTACG TCAGTGGAGA TATCACATCA ATCCACTTGC TTTGAAGACG TGGTTGGAAC GTCTTCTTTT TCCACGATGC TCCTCGTGGG TGGGGGTCCA TCTTTGGGAC CACTGTCGGC AGAGGCATCT TGAACGATAG CCTTTCCTTT ATCGCAATGA TGGCATTTGT AGGTGCCACC TTCCTTTTCT ACTGTCCTTT TGATGAAGTG ACAGATAGCT GGGCAATGGA ATCCGAGGAG GTTTCCCGAT ATTACCCTTT GTTGAAAAGT CTCAATAGCC CTTTGGTCTT CTGAGACTGT ATCTTTGATA TTCTTGGAGT AGACGAGAGT GTCGTGCTCC ACCATGTTAT CACATCAATC CACTTGCTTT GAAGACGTGG TTGGAACGTC TTCTTTTTCC ACGATGCTCC TCGTGGGTGG GGGTCCATCT TTGGGACCAC TGTCGGCAGA GGCATCTTGA ACGATAGCCT TTCCTTTATC GCAATGATGG CATTTGTAGG TGCCACCTTC CTTTTCTACT GTCCTTTTGA TGAAGTGACA GATAGCTGGG CAATGGAATC CGAGGAGGTT TCCCGATATT ACCCTTTGTT GAAAAGTCTC AATAGCCCTT TGGTCTTCTG AGACTGTATC TTTGATATTC TTGGAGTAGA CGAGAGTGTC GTGCTCCACC ATGTTGGCAA GCTGCTCTAG CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGAATT CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GCTAGAGCAG CTTGAGCTTG GATCAGATTG TCGTTTCCCG CCTTCAGTTT AGCTTCATGG AGTCAAAGAT TCAAATAGAG GACCTAACAG AACTCGCCGT AAAGACTGGC GAACAGTTCA TACAGAGTCT CTTACGACTC AATGACAAGA AGAAAATCTT CGTCAACATG GTGGAGCACG ACACACTTGT CTACTCCAAA AATATCAAAG ATACAGTCTC AGAAGACCAA AGGGCAATTG AGACTTTTCA ACAAAGGGTA ATATCCGGAA ACCTCCTCGG ATTCCATTGC CCAGCTATCT GTCACTTTAT TGTGAAGATA GTGGAAAAGG AAGGTGGCTC CTACAAATGC CATCATTGCG ATAAAGGAAA GGCCATCGTT GAAGATGCCT CTGCCGACAG TGGTCCCAAA GATGGACCCC CACCCACGAG GAGCATCGTG GAAAAAGAAG ACGTTCCAAC CACGTCTTCA AAGCAAGTGG ATTGATGTGA TATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ACCCTTCCTC TATATAAGGA AGTTCATTTC ATTTGGAGAG AACACGGGGG ACTCTTGAC
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