pMG36e
$128.00
In stock
SKU
PL83018
100μg liquid plasmid(Instantly):$163
| name | pMG36e | another name | apMG36e |
| Insert Size (bp) | Plasmid host | lactic acid bacteria | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Ery |
| Selective marker | Ery | Fluorescent labeling | |
| Promoter | P32 | Replicon | pWV01 |
| Competence | MC1061 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | P32-F：CCTCTGACTTGAGCGTCGAT | |
| 3′ sequencing primer | repA-R：CCTCTGACTTGAGCGTCGAT | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGTGATTTCA GAATCGAAAA AAAGAGTTAT GATTTCTCTG ACAAAAGAGC AAGATAAAAA ATTAACAGAT ATGGCGAAAC AAAAAGGTTT TTCAAAATCT GCGGTTGCGG CGTTAGCTAT AGAAGAATAT GCAAGAAAGG AATCAGAACA AAAAAAATAA GCGAAAGCTC GCGTTTTTAG AAGGATACGA GTTTTCGCTA CTTGTTTTTG ATAAGGTAAT TATATCATGG CTATTAAAAA TACTAAAGCT AGAAATTTTG GATTTTTATT ATATCCTGAC TCAATTCCTA ATGATTGGAA AGAAAAATTA GAGAGTTTGG GCGTATCTAT GGCTGTCAGT CCTTTACACG ATATGGACGA AAAAAAAGAT AAAGATACAT GGAATAGTAG TGATGTTATA CGAAATGGAA AGCACTATAA AAAACCACAC TATCACGTTA TATATATTGC ACGAAATCCT GTAACAATAG AAAGCGTTAG GAACAAGATT AAGCGAAAAT TGGGGAATAG TTCAGTTGCT CATGTTGAGA TACTTGATTA TATCAAAGGT TCATATGAAT ATTTGACTCA TGAATCAAAG GACGCTATTG CTAAGAATAA ACATATATAC GACAAAAAAG ATATTTTGAA CATTAATGAT TTTGATATTG ACCGCTATAT AACACTTGAT GAAAGCCAAA AAAGAGAATT GAAGAATTTA CTTTTAGATA TAGTGGATGA CTATAATTTG GTAAATACAA AAGATTTAAT GGCTTTTATT CGCCTTAGGG GAGCGGAGTT TGGAATTTTA AATACGAATG ATGTAAAAGA TATTGTTTCA ACAAACTCTA GCGCCTTTAG ATTATGGTTT GAGGGCAATT ATCAGTGTGG ATATAGAGCA AGTTATGCAA AGGTTCTTGA TGCTGAAACG GGGGAAATAA AATGACAAAC AAAGAAAAAG AGTTATTTGC TGAAAATGAG GAATTAAAAA AAGAAATTAA GGACTTAAAA GAGCGTATTG AAAGATACAG AGAAATGGAA GTTGAATTAA GTACAACAAT AGATTTATTG AGAGGAGGGA TTATTGAATA AATAAAAGCC CCCTGACGAA AGTCGAAGGG GGTTTTTATT TTGGTTTGAT GTTGCGATTA ATAGCAATAC AATTGCAATA AACAAAATGA TCGACCTCGG GACCCCTATC TAGCGAACTT TTAGAAAAGA TATAAAACAT CAGAGTATGG ACAGTTGCGG ATGTACTTCA GAAAAGATTA GATGTCTAAA AAGCTAGCTT TTTAGACATC TAAATCTAGG TACTAAAACA ATTCATCCAG TAAAATATAA TATTTTATTT TCTCCCAATC AGGCTTGATC CCCAGTAAGT CAAAAAATAG CTCGACATAC TGTTCTTCCC CGATCGACCC GATTCACAAA AAATAGGCAC ACGAAAAACA AGTTAAGGGA TGCAGTTTAT GCATCCCTTA ACTTACTTAT TAAATAATTT ATAGCTATTG AAAAGAGATA AGAATTGTTC AAAGCTAATA TTGTTTAAAT CGTCAATTCC TGCATGTTTT AAGGAATTGT TAAATTGATT TTTTGTAAAT ATTTTCTTGT ATTCTTTGTT AACCCATTTC ATAACGAAAT AATTATACTT TTGTTTATCT TTGTGTGATA TTCTTGATTT TTTTCTACTT AATCTGATAA GTGAGCTATT CACTTTAGGT TTAGGATGAA AATATTCTCT TGGAACCATA CTTAATATAG AAATATCAAC TTCTGCCATT AAAAGTAATG CCAATGAGCG TTTTGTATTT AATAATCTTT TAGCAAACCC GTATTCCACG ATTAAATAAA TCTCATTAGC TATACTATCA AAAACAATTT TGCGTATTAT ATCCGTACTT ATGTTATAAG GTATATTACC ATATATTTTA TAGGATTGGT TTTTAGGAAA TTTAAACTGC AATATATCCT TGTTTAAAAC TTGGAAATTA TCGTGATCAA CAAGTTTATT TTCTGTAGTT TTGCATAATT TATGGTCTAT TTCAATGGCA GTTACGAAAT TACACCTCTT TACTAATTCA AGGGTAAAAT GGCCTTTTCC TGAGCCGATT TCAAAGATAT TATCATGTTC ATTTAATCTT ATATTTGTCA TTATTTTATC TATATTATGT TTTGAAGTAA TAAAGTTTTG ACTGTGTTTT ATATTTTTCT CGTTCATTAT AACCCTCTTT AATTTGGTTA TATGAATTTT GCTTATTAAC GATTCATTAT AACCACTTAT TTTTTGTTTG GTTGATAATG AACTGTGCTG ATTACAAAAA TACTAAAAAT GCCCATATTT TTTCCTCCTT ATAAAATTAG TATAATTATA GCACGGTCGA TCTTCTATAT AAAAGATATA TTATCTTATC AGTATTGTCA ATATATTCAA GGCAATCTGC CTCCTCATCC TCTTCATCCT CTTCGTCTTG GTAGCTTTTT AAATATGGGT CGATCGAATT CGGTCCTCGG GATATGATAA GATTAATAGT TTTAGCTATT AATCTTTTTT TATTTTTATT TAAGAATGGC TTAATAAAGC GGTTACTTTG GATTTTTGTG AGCTTGGACT AGAAAAAAAC TTCACAAAAT GCTATACTAG GTAGGTAAAA AAATATTCGG AGGAATTTTG AAATGGCAAT CGTTTCAGCA GAAAAATTCG TAATTCGAGC TCGCCCGGGG ATCGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTTG CAAAGTCTGA AAACGAAGGT GGCAGCTGCC GTTGAAGCGG CCAAGACAGT TGGTAAAGGC GACGGTACAA CCGGTACTAG CGACAAAGGC GGCGGTCAAG GTACCCCGGC GCTACGATAT TTGGAGTTGA GGTTCAAAGT CAAATGGTAC TGATGACCGG TAAAATTTAA TATTTTGAAC CTTGCTTAGG CAGCTGACTT CACATTGTTG AGATCAGCTG CCTTTTGCTT ATAGTTCATT GAGTAGAAAC GGTTCTGTTG CGAAGTTTGA AAATCAAACA AGCTCGATTT TTTATTAAAA CGTCTCAAAA TCGTTTCTGA GACGTTTTAG CGTTTATTTC GTTTAGTTAT CGGCATAATC GTTAAAACAG GCGTTATCGT AGCGTAAAAG CCCTTGAGCG TAGCGTGGCT TTGCAGGAAG ATGTTGTCTG TTAGATTATG AAAGCCGATG ACTGAATGAA ATAATAAGCG CAGCGCCCTT CTATTTCGGT TGGAGGAGGC TCAAGGGAGT ATGAGGGAAT GAAATTCCCT CATGGGTTTG ATTTTAAAAA TTGCTTGCAA TTTTGCCGAG CGGTAGCGCT GGAAAATTTT TGAAAAAAAT TTGGAATTTG GAAAAAAATG GGGGGAAAGG AAGCGAATTT TGCTTCCGTA CTACGACCCC CCATTAAGTG CCGAGTGCCA ATTTTTGTGC CAAAAACGCT CTATCCCAAC TGGCTCAAGG GTTTAAGGGG TTTTTCAATC GCCAACGAAT CGCCAACGTT TTCGCCAACG TTTTTTATAA ATCTATATTT AAGTAGCTTT ATTGTTGTTT TTATGATTAC AAAGTGATAC ACTAACTTTA TAAAATTATT TGATTGGAGT TTTTTAAT
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