pER8
$157.00
In stock
SKU
PL83011
雌二醇诱导
100μg liquid plasmid(Instantly):$178
| name | pER8 | another name | apER8 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Spe | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 35S：GACGCACAATCCCACTATCC | |
| 3′ sequencing primer | NOS-R：GATAATCATCGCAAGACCGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TAAGCTTGCA TGCCTGCAGG ATATCGTGGA TCCAAGCTTG CCACGTGCCG CCACGTGCCG CCACGTGCCG CCACGTGCCT CTAGAGGATC CATCTCCACT GACGTAAGGG ATGACGCACA ATCCCACTAT CCTTCGCAAG ACCCTTCCTC TATATAAGGA AGTTCATTTC ATTTGGAGAG GACACGCTGG GATCCCCAAT TCCGGGCGGA ATGAAAGCGT TAACGGCCAG GCAACAAGAG GTGTTTGATC TCATCCGTGA TCACATCAGC CAGACAGGTA TGCCGCCGAC GCGTGCGGAA ATCGCGCAGC GTTTGGGGTT CCGTTCCCCA AACGCGGCTG AAGAACATCT GAAGGCGCTG GCACGCAAAG GCGTTATTGA AATTGTTTCC GGCGCATCAC GCGGGATTCG TCTGTTGCAG GAAGAGGAAG AAGGGTTGCC GCTGGTAGGT CGTGTGGCTG CCGGTGAACC GTCGAGCGCC CCCCCGACCG ATGTCAGCCT GGGGGACGAG CTCCACTTAG ACGGCGAGGA CGTGGCGATG GCGCATGCCG ACGCGCTAGA CGATTTCGAT CTGGACATGT TGGGGGACGG GGATTCCCCG GGTCCGGGAT TTACCCCCCA CGACTCCGCC CCCTACGGCG CTCTGGATAT GGCCGACTTC GAGTTTGAGC AGATGTTTAC CGATGCCCTT GGAATTGACG AGTACGGTGG GGATCCGTCT GCTGGAGACA TGAGAGCTGC CAACCTTTGG CCAAGCCCGC TCATGATCAA ACGCTCTAAG AAGAACAGCC TGGCCTTGTC CCTGACGGCC GACCAGATGG TCAGTGCCTT GTTGGATGCT GAGCCCCCCA TACTCTATTC CGAGTATGAT CCTACCAGAC CCTTCAGTGA AGCTTCGATG ATGGGCTTAC TGACCAACCT GGCAGACAGG GAGCTGGTTC ACATGATCAA CTGGGCGAAG AGGGTGCCAG GCTTTGTGGA TTTGACCCTC CATGATCAGG TCCACCTTCT AGAATGTGCC TGGCTAGAGA TCCTGATGAT TGGTCTCGTC TGGCGCTCCA TGGAGCACCC AGTGAAGCTA CTGTTTGCTC CTAACTTGCT CTTGGACAGG AACCAGGGAA AATGTGTAGA GGGCATGGTG GAGATCTTCG ACATGCTGCT GGCTACATCA TCTCGGTTCC GCATGATGAA TCTGCAGGGA GAGGAGTTTG TGTGCCTCAA ATCTATTATT TTGCTTAATT CTGGAGTGTA CACATTTCTG TCCAGCACCC TGAAGTCTCT GGAAGAGAAG GACCATATCC ACCGAGTCCT GGACAAGATC ACAGACACTT TGATCCACCT GATGGCCAAG GCAGGCCTGA CCCTGCAGCA GCAGCACCAG CGGCTGGCCC AGCTCCTCCT CATCCTCTCC CACATCAGGC ACATGAGTAA CAAAGGCATG GAGCATCTGT ACAGCATGAA GTGCAAGAAC GTGGTGCCCC TCTATGACCT GCTGCTGGAG ATGCTGGACG CCCACCGCCT ACATGCGCCC ACTAGCCGTG GAGGGGCATC CGTGGAGGAG ACGGACCAAA GCCACTTGGC CACTGCGGGC TCTACTTCAT CGCATTCCTT GCAAAAGTAT TACATCACGG GGGAGGCAGA GGGTTTCCCT GCCACAGTCT GAGAGCTCCC TGGCGAATTC CCAGAGATGT TAGCTGAAAT CATCACTAAT CAGATACCAA AATATTCAAA TGGAAATATC AAAAAGCTTC TGTTTCATCA AAAATGACTC GACCTAACTG AGTAAGCTAG CTTGTTCGAG TATTATGGCA TTGGGAAAAC TGTTTTTCTT GTACCATTTG TTGTGCTTGT AATTTACTGT GTTTTTTATT CGGTTTTCGC TATCGAACTG TGAAATGGAA ATGGATGGAG AAGAGTTAAT GAATGATATG GTCCTTTTGT TCATTCTCAA ATTAATATTA TTTGTTTTTT CTCTTATTTG TTGTGTGTTG AATTTGAAAT TATAAGAGAT ATGCAAACAT TTTGTTTTGA GTAAAAATGT GTCAAATCGT GGCCTCTAAT GACCGAAGTT AATATGAGGA GTAAAACATC CCAAACAAGC TTGGAAACTG AAGGCGGGAA ACGACAATCT GATCATGAGC GGAGAATTAA GGGAGTCACG TTATGACCCC CGCCGATGAC GCGGGACAAG CCGTTTTACG TTTGGAACTG ACAGAACCGC AACGATTGAA GGAGCCACTC AGCCGCGGGT TTCTGGAGTT TAATGAGCTA AGCACATACG TCAGAAACCA TTATTGCGCG TTCAAAAGTC GCCTAAGGTC ACTATCAGCT AGCAAATATT TCTTGTCAAA AATGCTCCAC TGACGTTCCA TAAATTCCCC TCGGTATCCA ATTAGAGTCT CATATTCACT CTCAATCCAA ATAATCTGCA CCGGATCCCC TAGAATGAAA AAGCCTGAAC TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAA TTCAGCGAGA GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC CGCTGTTCTG CAGCCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG AGGACTGCCC CGAAGTCCGG CACCTCGTGC ACGCGGATTT CGGCTCCAAC AATGTCCTGA CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC AATACGAGGT CGCCAACATC TTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA CGCGCTACTT CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCGCGGCTC CGGGCGTATA TGCTCCGCAT TGGTCTTGAC CAACTCTATC AGAGCTTGGT TGACGGCAAT TTCGATGATG CAGCTTGGGC GCAGGGTCGA TGCGACGCAA TCGTCCGATC CGGAGCCGGG ACTGTCGGGC GTACACAAAT CGCCCGCAGA AGCGCGGCCG TCTGGACCGA TGGCTGTGTA GAAGTACTCG CCGATAGTGG AAACCGACGC CCCAGCACTC GTCCGAGGGC AAAGGAATAG CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGGAA TTGATCCCCC CTCGACAGCT TGCATGCCAG CTTGGGCTGC AGGTCGAGGC TAAAAAACTA ATCGCATTAT CATCCCCTCG ACGTACTGTA CATATAACCA CTGGTTTTAT ATACAGCAGT ACTGTACATA TAACCACTGG TTTTATATAC AGCAGTCGAC GTACTGTACA TATAACCACT GGTTTTATAT ACAGCAGTAC TGTACATATA ACCACTGGTT TTATATACAG CAGTCGAGGT AAGATTAGAT ATGGATATGT ATATGGATAT GTATATGGTG GTAATGCCAT GTAATATGCT CGACTCTAGG ATCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GGACACGCTG AAGCTAGTCG ACTCTAGCCT CGAGGCGCGC CGGGCCCATA CGCGTTAATT AACTAGTCGA TCCAGGCCTC CCAGCTTTCG TCCGTATCAT CGGTTTCGAC AACGTTCGTC AAGTTCAATG CATCAGTTTC ATTGCCCACA CACCAGAATC CTACTAAGTT TGAGTATTAT GGCATTGGAA AAGCTGTTTT CTTCTATCAT TTGTTCTGCT TGTAATTTAC TGTGTTCTTT CAGTTTTTGT TTTCGGACAT CAAAATGCAA ATGGATGGAT AAGAGTTAAT AAATGATATG GTCCTTTTGT TCATTCTCAA ATTATTATTA TCTGTTGTTT TTACTTTAAT GGGTTGAATT TAAGTAAGAA AGGAACTAAC AGTGTGATAT TAAGGTGCAA TGTTAGACAT ATAAAACAGT CTTTCACCTC TCTTTGGTTA TGTCTTGAAT TGGTTTGTTT CTTCACTTAT CTGTGTAATC AAGTTTACTA TGAGTCTATG ATCAAGTAAT TATGCAATCA AGTTAAGTAC AGTATAGGCT TTTTGTGTCG AGGGGGTACC GAGTCGAGGA ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGGGTA CCGAGCTCGA ATTCAATTCG GCGTTAATTC AGTACATTAA AAACGTCCGC AATGTGTTAT TAAGTTGTCT AAGCGTCAAT TTGTTTACAC CACAATATAT CCTGCCACCA GCCAGCCAAC AGCTCCCCGA CCGGCAGCTC GGCACAAAAT CACCACTCGA TACAGGCAGC CCATCAGTCC GGGACGGCGT CAGCGGGAGA GCCGTTGTAA GGCGGCAGAC TTTGCTCATG TTACCGATGC TATTCGGAAG AACGGCAACT AAGCTGCCGG GTTTGAAACA CGGATGATCT CGCGGAGGGT AGCATGTTGA TTGTAACGAT GACAGAGCGT TGCTGCCTGT GATCAATTCG GGCACGAACC CAGTGGACAT AAGCCTCGTT CGGTTCGTAA GCTGTAATGC AAGTAGCGTA ACTGCCGTCA CGCAACTGGT CCAGAACCTT GACCGAACGC AGCGGTGGTA ACGGCGCAGT GGCGGTTTTC ATGGCTTCTT GTTATGACAT GTTTTTTTGG GGTACAGTCT ATGCCTCGGG CATCCAAGCA GCAAGCGCGT TACGCCGTGG GTCGATGTTT GATGTTATGG AGCAGCAACG ATGTTACGCA GCAGGGCAGT CGCCCTAAAA CAAAGTTAAA CATCATGGGG GAAGCGGTGA TCGCCGAAGT ATCGACTCAA CTATCAGAGG TAGTTGGCGT CATCGAGCGC CATCTCGAAC CGACGTTGCT GGCCGTACAT TTGTACGGCT CCGCAGTGGA TGGCGGCCTG AAGCCACACA GTGATATTGA TTTGCTGGTT ACGGTGACCG TAAGGCTTGA TGAAACAACG CGGCGAGCTT TGATCAACGA CCTTTTGGAA ACTTCGGCTT CCCCTGGAGA GAGCGAGATT CTCCGCGCTG TAGAAGTCAC CATTGTTGTG CACGACGACA TCATTCCGTG GCGTTATCCA GCTAAGCGCG AACTGCAATT TGGAGAATGG CAGCGCAATG ACATTCTTGC AGGTATCTTC GAGCCAGCCA CGATCGACAT TGATCTGGCT ATCTTGCTGA CAAAAGCAAG AGAACATAGC GTTGCCTTGG TAGGTCCAGC GGCGGAGGAA CTCTTTGATC CGGTTCCTGA ACAGGATCTA TTTGAGGCGC TAAATGAAAC CTTAACGCTA TGGAACTCGC CGCCCGACTG GGCTGGCGAT GAGCGAAATG TAGTGCTTAC GTTGTCCCGC ATTTGGTACA GCGCAGTAAC CGGCAAAATC GCGCCGAAGG ATGTCGCTGC CGACTGGGCA ATGGAGCGCC TGCCGGCCCA GTATCAGCCC GTCATACTTG AAGCTAGACA GGCTTATCTT GGACAAGAAG AAGATCGCTT GGCCTCGCGC GCAGATCAGT TGGAAGAATT TGTCCACTAC GTGAAAGGCG AGATCACCAA GGTAGTCGGC AAATAATGTC TAGCTAGAAA TTCGTTCAAG CCGACGCCGC TTCGCCGGCG TTAACTCAAG CGATTAGATG CACTAAGCAC ATAATTGCTC ACAGCCAAAC TATCAGGTCA AGTCTGCTTT TATTATTTTT AAGCGTGCAT AATAAGCCCT ACACAAATTG GGAGATATAT CATGCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGGGTGC CTTGATGTGG GCGCCGGCGG TCGAGTGGCG ACGGCGCGGC TTGTCCGCGC CCTGGTAGAT TGCCTGGCCG TAGGCCAGCC ATTTTTGAGC GGCCAGCGGC CGCGATAGGC CGACGCGAAG CGGCGGGGCG TAGGGAGCGC AGCGACCGAA GGGTAGGCGC TTTTTGCAGC TCTTCGGCTG TGCGCTGGCC AGACAGTTAT GCACAGGCCA GGCGGGTTTT AAGAGTTTTA ATAAGTTTTA AAGAGTTTTA GGCGGAAAAA TCGCCTTTTT TCTCTTTTAT ATCAGTCACT TACATGTGTG ACCGGTTCCC AATGTACGGC TTTGGGTTCC CAATGTACGG GTTCCGGTTC CCAATGTACG GCTTTGGGTT CCCAATGTAC GTGCTATCCA CAGGAAAGAG ACCTTTTCGA CCTTTTTCCC CTGCTAGGGC AATTTGCCCT AGCATCTGCT CCGTACATTA GGAACCGGCG GATGCTTCGC CCTCGATCAG GTTGCGGTAG CGCATGACTA GGATCGGGCC AGCCTGCCCC GCCTCCTCCT TCAAATCGTA CTCCGGCAGG TCATTTGACC CGATCAGCTT GCGCACGGTG AAACAGAACT TCTTGAACTC TCCGGCGCTG CCACTGCGTT CGTAGATCGT CTTGAACAAC CATCTGGCTT CTGCCTTGCC TGCGGCGCGG CGTGCCAGGC GGTAGAGAAA ACGGCCGATG CCGGGATCGA TCAAAAAGTA ATCGGGGTGA ACCGTCAGCA CGTCCGGGTT CTTGCCTTCT GTGATCTCGC GGTACATCCA ATCAGCTAGC TCGATCTCGA TGTACTCCGG CCGCCCGGTT TCGCTCTTTA CGATCTTGTA GCGGCTAATC AAGGCTTCAC CCTCGGATAC CGTCACCAGG CGGCCGTTCT TGGCCTTCTT CGTACGCTGC ATGGCAACGT GCGTGGTGTT TAACCGAATG CAGGTTTCTA CCAGGTCGTC TTTCTGCTTT CCGCCATCGG CTCGCCGGCA GAACTTGAGT ACGTCCGCAA CGTGTGGACG GAACACGCGG CCGGGCTTGT CTCCCTTCCC TTCCCGGTAT CGGTTCATGG ATTCGGTTAG ATGGGAAACC GCCATCAGTA CCAGGTCGTA ATCCCACACA CTGGCCATGC CGGCCGGCCC TGCGGAAACC TCTACGTGCC CGTCTGGAAG CTCGTAGCGG ATCACCTCGC CAGCTCGTCG GTCACGCTTC GACAGACGGA AAACGGCCAC GTCCATGATG CTGCGACTAT CGCGGGTGCC CACGTCATAG AGCATCGGAA CGAAAAAATC TGGTTGCTCG TCGCCCTTGG GCGGCTTCCT AATCGACGGC GCACCGGCTG CCGGCGGTTG CCGGGATTCT TTGCGGATTC GATCAGCGGC CGCTTGCCAC GATTCACCGG GGCGTGCTTC TGCCTCGATG CGTTGCCGCT GGGCGGCCTG CGCGGCCTTC AACTTCTCCA CCAGGTCATC ACCCAGCGCC GCGCCGATTT GTACCGGGCC GGATGGTTTG CGACCGTCAC GCCGATTCCT CGGGCTTGGG GGTTCCAGTG CCATTGCAGG GCCGGCAGAC AACCCAGCCG CTTACGCCTG GCCAACCGCC CGTTCCTCCA CACATGGGGC ATTCCACGGC GTCGGTGCCT GGTTGTTCTT GATTTTCCAT GCCGCCTCCT TTAGCCGCTA AAATTCATCT ACTCATTTAT TCATTTGCTC ATTTACTCTG GTAGCTGCGC GATGTATTCA GATAGCAGCT CGGTAATGGT CTTGCCTTGG CGTACCGCGT ACATCTTCAG CTTGGTGTGA TCCTCCGCCG GCAACTGAAA GTTGACCCGC TTCATGGCTG GCGTGTCTGC CAGGCTGGCC AACGTTGCAG CCTTGCTGCT GCGTGCGCTC GGACGGCCGG CACTTAGCGT GTTTGTGCTT TTGCTCATTT TCTCTTTACC TCATTAACTC AAATGAGTTT TGATTTAATT TCAGCGGCCA GCGCCTGGAC CTCGCGGGCA GCGTCGCCCT CGGGTTCTGA TTCAAGAACG GTTGTGCCGG CGGCGGCAGT GCCTGGGTAG CTCACGCGCT GCGTGATACG GGACTCAAGA ATGGGCAGCT CGTACCCGGC CAGCGCCTCG GCAACCTCAC CGCCGATGCG CGTGCCTTTG ATCGCCCGCG ACACGACAAA GGCCGCTTGT AGCCTTCCAT CCGTGACCTC AATGCGCTGC TTAACCAGCT CCACCAGGTC GGCGGTGGCC CATATGTCGT AAGGGCTTGG CTGCACCGGA ATCAGCACGA AGTCGGCTGC CTTGATCGCG GACACAGCCA AGTCCGCCGC CTGGGGCGCT CCGTCGATCA CTACGAAGTC GCGCCGGCCG ATGGCCTTCA CGTCGCGGTC AATCGTCGGG CGGTCGATGC CGACAACGGT TAGCGGTTGA TCTTCCCGCA CGGCCGCCCA ATCGCGGGCA CTGCCCTGGG GATCGGAATC GACTAACAGA ACATCGGCCC CGGCGAGTTG CAGGGCGCGG GCTAGATGGG TTGCGATGGT CGTCTTGCCT GACCCGCCTT TCTGGTTAAG TACAGCGATA ACCTTCATGC GTTCCCCTTG CGTATTTGTT TATTTACTCA TCGCATCATA TACGCAGCGA CCGCATGACG CAAGCTGTTT TACTCAAATA CACATCACCT TTTTAGACGG CGGCGCTCGG TTTCTTCAGC GGCCAAGCTG GCCGGCCAGG CCGCCAGCTT GGCATCAGAC AAACCGGCCA GGATTTCATG CAGCCGCACG GTTGAGACGT GCGCGGGCGG CTCGAACACG TACCCGGCCG CGATCATCTC CGCCTCGATC TCTTCGGTAA TGAAAAACGG TTCGTCCTGG CCGTCCTGGT GCGGTTTCAT GCTTGTTCCT CTTGGCGTTC ATTCTCGGCG GCCGCCAGGG CGTCGGCCTC GGTCAATGCG TCCTCACGGA AGGCACCGCG CCGCCTGGCC TCGGTGGGCG TCACTTCCTC GCTGCGCTCA AGTGCGCGGT ACAGGGTCGA GCGATGCACG CCAAGCAGTG CAGCCGCCTC TTTCACGGTG CGGCCTTCCT GGTCGATCAG CTCGCGGGCG TGCGCGATCT GTGCCGGGGT GAGGGTAGGG CGGGGGCCAA ACTTCACGCC TCGGGCCTTG GCGGCCTCGC GCCCGCTCCG GGTGCGGTCG ATGATTAGGG AACGCTCGAA CTCGGCAATG CCGGCGAACA CGGTCAACAC CATGCGGCCG GCCGGCGTGG TGGTGTCGGC CCACGGCTCT GCCAGGCTAC GCAGGCCCGC GCCGGCCTCC TGGATGCGCT CGGCAATGTC CAGTAGGTCG CGGGTGCTGC GGGCCAGGCG GTCTAGCCTG GTCACTGTCA CAACGTCGCC AGGGCGTAGG TGGTCAAGCA TCCTGGCCAG CTCCGGGCGG TCGCGCCTGG TGCCGGTGAT CTTCTCGGAA AACAGCTTGG TGCAGCCGGC CGCGTGCAGT TCGGCCCGTT GGTTGGTCAA GTCCTGGTCG TCGGTGCTGA CGCGGGCATA GCCCAGCAGG CCAGCGGCGG CGCTCTTGTT CATGGCGTAA TGTCTCCGGT TCTAGTCGCA AGTATTCTAC TTTATGCGAC TAAAACACGC GACAAGAAAA CGCCAGGAAA AGGGCAGGGC GGCAGCCTGT CGCGTAACTT AGGACTTGTG CGACATGTCG TTTTCAGAAG ACGGCTGCAC TGAACGTCAG AAGCCGACTG CACTATAGCA GCGGAGGGGT TGGATCAAAG TACTTTGATC CCGAGGGGAA CCCTGTGGTT GGCATGCACA TACAAATGGA CGAACGGATA AACCTTTTCA CGCCCTTTTA AATATCCGTT ATTCTAATAA ACGCTCTTTT CTCTTAG
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