pBBR1MCS-2
$99.00
In stock
SKU
PL82999
pBBR1MCS2，提取时要加诱导剂
100μg liquid plasmid(Instantly):$178
| name | pBBR1MCS-2 | another name | apBBR1MCS-2 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pBBR1 | |
| Competence | EPI300 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13R：CAGGAAACAGCTATGACC | |
| 3′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | Copy number | Extremely low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACCTTCGGGA GCGCCTGAAG CCCGTTCTGG ACGCCCTGGG GCCGTTGAAT CGGGATATGC AGGCCAAGGC CGCCGCGATC ATCAAGGCCG TGGGCGAAAA GCTGCTGACG GAACAGCGGG AAGTCCAGCG CCAGAAACAG GCCCAGCGCC AGCAGGAACG CGGGCGCGCA CATTTCCCCG AAAAGTGCCA CCTGGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA ATTACAACAG TTTTTATGCA TGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TGGGTACCGG GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG ATCCACTAGT TCTAGAGCGG CCGCCACCGC GGTGGAGCTC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GACTGCGATG AGTGGCAGGG CGGGGCGTAA TTTTTTTAAG GCAGTTATTG GTGCCCTTAA ACGCCTGGTG CTACGCCTGA ATAAGTGATA ATAAGCGGAT GAATGGCAGA AATTCGAAAG CAAATTCGAC CCGGTCGTCG GTTCAGGGCA GGGTCGTTAA ATAGCCGCTT ATGTCTATTG CTGGTTTACC GGTTTATTGA CTACCGGAAG CAGTGTGACC GTGTGCTTCT CAAATGCCTG AGGCCAGTTT GCTCAGGCTC TCCCCGTGGA GGTAATAATT GACGATATGA TCATTTATTC TGCCTCCCAG AGCCTGATAA AAACGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGA GGCGGCTACA GCCGATAGTC TGGAACAGCG CACTTACGGG TTGCTGCGCA ACCCAAGTGC TACCGGCGCG GCAGCGTGAC CCGTGTCGGC GGCTCCAACG GCTCGCCATC GTCCAGAAAA CACGGCTCAT CGGGCATCGG CAGGCGCTGC TGCCCGCGCC GTTCCCATTC CTCCGTTTCG GTCAAGGCTG GCAGGTCTGG TTCCATGCCC GGAATGCCGG GCTGGCTGGG CGGCTCCTCG CCGGGGCCGG TCGGTAGTTG CTGCTCGCCC GGATACAGGG TCGGGATGCG GCGCAGGTCG CCATGCCCCA ACAGCGATTC GTCCTGGTCG TCGTGATCAA CCACCACGGC GGCACTGAAC ACCGACAGGC GCAACTGGTC GCGGGGCTGG CCCCACGCCA CGCGGTCATT GACCACGTAG GCCGACACGG TGCCGGGGCC GTTGAGCTTC ACGACGGAGA TCCAGCGCTC GGCCACCAAG TCCTTGACTG CGTATTGGAC CGTCCGCAAA GAACGTCCGA TGAGCTTGGA AAGTGTCTTC TGGCTGACCA CCACGGCGTT CTGGTGGCCC ATCTGCGCCA CGAGGTGATG CAGCAGCATT GCCGCCGTGG GTTTCCTCGC AATAAGCCCG GCCCACGCCT CATGCGCTTT GCGTTCCGTT TGCACCCAGT GACCGGGCTT GTTCTTGGCT TGAATGCCGA TTTCTCTGGA CTGCGTGGCC ATGCTTATCT CCATGCGGTA GGGTGCCGCA CGGTTGCGGC ACCATGCGCA ATCAGCTGCA ACTTTTCGGC AGCGCGACAA CAATTATGCG TTGCGTAAAA GTGGCAGTCA ATTACAGATT TTCTTTAACC TACGCAATGA GCTATTGCGG GGGGTGCCGC AATGAGCTGT TGCGTACCCC CCTTTTTTAA GTTGTTGATT TTTAAGTCTT TCGCATTTCG CCCTATATCT AGTTCTTTGG TGCCCAAAGA AGGGCACCCC TGCGGGGTTC CCCCACGCCT TCGGCGCGGC TCCCCCTCCG GCAAAAAGTG GCCCCTCCGG GGCTTGTTGA TCGACTGCGC GGCCTTCGGC CTTGCCCAAG GTGGCGCTGC CCCCTTGGAA CCCCCGCACT CGCCGCCGTG AGGCTCGGGG GGCAGGCGGG CGGGCTTCGC CTTCGACTGC CCCCACTCGC ATAGGCTTGG GTCGTTCCAG GCGCGTCAAG GCCAAGCCGC TGCGCGGTCG CTGCGCGAGC CTTGACCCGC CTTCCACTTG GTGTCCAACC GGCAAGCGAA GCGCGCAGGC CGCAGGCCGG AGGCTTTTCC CCAGAGAAAA TTAAAAAAAT TGATGGGGCA AGGCCGCAGG CCGCGCAGTT GGAGCCGGTG GGTATGTGGT CGAAGGCTGG GTAGCCGGTG GGCAATCCCT GTGGTCAAGC TCGTGGGCAG GCGCAGCCTG TCCATCAGCT TGTCCAGCAG GGTTGTCCAC GGGCCGAGCG AAGCGAGCCA GCCGGTGGCC GCTCGCGGCC ATCGTCCACA TATCCACGGG CTGGCAAGGG AGCGCAGCGA CCGCGCAGGG CGAAGCCCGG AGAGCAAGCC CGTAGGGCGC CGCAGCCGCC GTAGGCGGTC ACGACTTTGC GAAGCAAAGT CTAGTGAGTA TACTCAAGCA TTGAGTGGCC CGCCGGAGGC ACCGCCTTGC GCTGCCCCCG TCGAGCCGGT TGGACACCAA AAGGGAGGGG CAGGCATGGC GGCATACGCG ATCATGCGAT GCAAGAAGCT GGCGAAAATG GGCAACGTGG CGGCCAGTCT CAAGCACGCC TACCGCGAGC GCGAGACGCC CAACGCTGAC GCCAGCAGGA CGCCAGAGAA CGAGCACTGG GCGGCCAGCA GCACCGATGA AGCGATGGGC CGACTGCGCG AGTTGCTGCC AGAGAAGCGG CGCAAGGACG CTGTGTTGGC GGTCGAGTAC GTCATGACGG CCAGCCCGGA ATGGTGGAAG TCGGCCAGCC AAGAACAGCA GGCGGCGTTC TTCGAGAAGG CGCACAAGTG GCTGGCGGAC AAGTACGGGG CGGATCGCAT CGTGACGGCC AGCATCCACC GTGACGAAAC CAGCCCGCAC ATGACCGCGT TCGTGGTGCC GCTGACGCAG GACGGCAGGC TGTCGGCCAA GGAGTTCATC GGCAACAAAG CGCAGATGAC CCGCGACCAG ACCACGTTTG CGGCCGCTGT GGCCGATCTA GGGCTGCAAC GGGGCATCGA GGGCAGCAAG GCACGTCACA CGCGCATTCA GGCGTTCTAC GAGGCCCTGG AGCGGCCACC AGTGGGCCAC GTCACCATCA GCCCGCAAGC GGTCGAGCCA CGCGCCTATG CACCGCAGGG ATTGGCCGAA AAGCTGGGAA TCTCAAAGCG CGTTGAGACG CCGGAAGCCG TGGCCGACCG GCTGACAAAA GCGGTTCGGC AGGGGTATGA GCCTGCCCTA CAGGCCGCCG CAGGAGCGCG TGAGATGCGC AAGAAGGCCG ATCAAGCCCA AGAGACGGCC CGAG
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