pET22b-SaCas9
$128.00
In stock
SKU
PL82998
100μg liquid plasmid(Instantly):$149
| name | pET22b-SaCas9 | another name | apET22b-SaCas9 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | CRISPR,Cas9 |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | T7 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | T7：TAATACGACTCACTATAGGG | |
| 3′ sequencing primer | T7ter：TGCTAGTTATTGCTCAGCGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACATATGAAA TACCTGCTGC CGACCGCTGC TGCTGGTCTG CTGCTCCTCG CTGCCCAGCC GGCGATGGCC ATGGATATCG GAATTAATTC GGATCCGAAT TCGAGCTCCG TCGACAAGCT TGCGGCCGCA CTCGAGCACC ACCACCACCA CCACATGGGC CGTAAAAAAC GTCGCCAGCG CCGCCGCGGT GGTGGGAGCA CGTATCCATA TGATGTGCCG GACTACGCGG AGTTCGGCAG TATGGCACCG AAGAAAAAGC GTAAAGTAGG TATTCACGGT GTCCCCGCAG CAAAACGTAA TTACATTCTG GGACTGGATA TCGGTATTAC GTCAGTGGGG TACGGTATCA TTGATTACGA AACGCGTGAT GTAATTGACG CGGGTGTCCG TCTGTTCAAA GAAGCGAATG TAGAAAATAA TGAAGGACGC CGCAGCAAAC GTGGAGCCCG CCGTCTGAAG CGTCGTCGTC GTCACCGCAT CCAACGCGTC AAAAAACTGC TGTTTGATTA CAACTTGCTG ACCGATCACA GTGAACTGAG CGGAATCAAC CCGTATGAAG CCCGCGTGAA AGGGCTGAGC CAGAAACTGT CCGAGGAAGA ATTCAGCGCC GCGCTGCTCC ACCTGGCGAA ACGCCGCGGT GTGCACAATG TGAACGAAGT CGAAGAAGAC ACCGGCAACG AACTGTCAAC TAAAGAACAG ATCAGTCGCA ACAGCAAAGC CCTGGAAGAG AAATACGTGG CAGAATTACA GCTTGAACGC CTGAAAAAAG ACGGGGAAGT GCGTGGGAGC ATTAACCGTT TTAAGACTAG TGATTACGTG AAGGAAGCGA AACAGCTGTT GAAAGTCCAG AAAGCGTACC ACCAGCTGGA TCAATCATTC ATTGATACAT ATATCGATTT GCTGGAAACC CGCCGTACCT ATTATGAAGG CCCTGGCGAA GGCTCGCCTT TTGGTTGGAA AGATATTAAA GAATGGTATG AAATGCTGAT GGGTCACTGT ACTTACTTTC CGGAAGAACT GCGCTCCGTC AAGTATGCAT ATAATGCCGA TCTGTATAAC GCGCTGAATG ATCTGAATAA CCTGGTTATT ACCCGTGATG AAAATGAAAA GCTGGAATAC TATGAAAAAT TTCAAATTAT CGAAAATGTC TTTAAACAGA AAAAAAAACC GACACTCAAA CAAATCGCCA AAGAAATTCT GGTGAACGAG GAGGATATTA AAGGTTATCG CGTTACGTCA ACCGGCAAAC CGGAATTTAC GAACCTTAAA GTGTATCATG ATATTAAAGA CATTACCGCG CGCAAAGAGA TTATCGAGAA CGCCGAGCTG CTGGATCAGA TTGCGAAAAT TCTGACCATC TATCAATCCT CCGAAGACAT CCAAGAAGAA CTCACCAATT TAAATAGCGA GCTGACTCAG GAGGAGATCG AGCAAATCTC CAACCTGAAG GGCTACACCG GCACGCATAA CCTCAGCTTA AAAGCTATTA ATTTGATTCT GGACGAACTT TGGCACACTA ACGATAACCA AATTGCTATT TTCAATCGTC TGAAACTGGT ACCTAAGAAA GTGGATCTGT CGCAGCAAAA AGAAATTCCG ACCACCCTGG TGGATGACTT TATCCTTAGT CCGGTCGTAA AGCGTAGCTT TATTCAAAGC ATCAAAGTCA TCAACGCAAT CATCAAAAAA TATGGACTCC CAAATGATAT CATTATCGAA CTGGCACGTG AGAAAAACTC CAAAGACGCT CAGAAAATGA TCAACGAAAT GCAGAAACGT AATCGTCAGA CTAACGAACG TATCGAAGAG ATTATTCGTA CCACGGGCAA GGAAAACGCA AAGTATCTGA TCGAAAAAAT TAAGTTGCAT GACATGCAAG AAGGCAAATG TCTGTATAGC CTGGAAGCGA TTCCCCTGGA GGACCTGCTG AACAACCCGT TTAACTATGA GGTAGATCAT ATTATTCCAC GCAGTGTTAG CTTTGATAAC TCATTTAATA ACAAAGTCCT TGTGAAACAG GAAGAGAACA GTAAGAAAGG TAACCGTACT CCATTCCAGT ACTTATCTAG TTCTGATTCT AAAATCTCTT ATGAAACATT CAAAAAACAC ATTCTGAATC TGGCTAAGGG GAAAGGTCGC ATTAGCAAAA CGAAAAAAGA ATATTTGCTG GAAGAACGTG ATATTAATCG TTTTTCCGTG CAGAAAGACT TCATCAATCG TAATCTGGTG GATACCCGTT ACGCGACTCG CGGGCTGATG AACCTGTTAC GTTCATACTT CCGTGTTAAC AACCTGGACG TCAAGGTAAA GAGCATCAAT GGAGGCTTTA CCTCTTTCCT GCGCCGCAAA TGGAAATTTA AAAAAGAACG CAACAAAGGC TACAAGCATC ATGCCGAGGA TGCCCTGATC ATTGCAAATG CCGACTTCAT TTTCAAAGAA TGGAAAAAAC TGGATAAAGC AAAAAAAGTG ATGGAAAATC AGATGTTTGA GGAAAAGCAA GCAGAATCTA TGCCGGAGAT TGAAACGGAA CAGGAATACA AAGAAATCTT TATTACGCCT CACCAAATTA AACACATCAA AGATTTTAAA GACTATAAAT ATTCGCATCG TGTGGATAAA AAACCAAACC GCGAATTAAT TAATGATACA CTGTACTCTA CCCGTAAAGA TGATAAGGGA AACACCCTGA TTGTTAATAA CCTGAACGGC CTGTACGATA AAGATAACGA CAAGCTGAAA AAACTTATCA ACAAGTCGCC GGAGAAACTG CTCATGTATC ATCATGATCC ACAGACTTAT CAGAAACTGA AACTGATCAT GGAACAGTAT GGTGATGAAA AAAATCCGCT GTATAAGTAC TATGAAGAAA CAGGCAATTA CCTTACGAAA TACTCTAAAA AAGACAATGG TCCTGTGATT AAAAAAATTA AATACTACGG CAACAAACTG AACGCACATC TGGATATTAC CGACGATTAT CCAAACTCGC GTAATAAAGT TGTTAAACTC TCATTGAAGC CTTATCGTTT CGATGTATAT CTCGACAACG GTGTTTACAA ATTCGTGACC GTGAAGAATC TTGATGTTAT CAAAAAAGAA AATTACTACG AAGTTAACTC TAAATGCTAT GAGGAGGCGA AAAAATTGAA AAAAATCTCT AATCAAGCCG AATTTATTGC AAGCTTTTAT AACAATGATC TGATCAAAAT TAACGGGGAG CTGTATCGCG TCATTGGTGT GAACAACGAT TTGCTCAATC GCATTGAAGT AAACATGATT GACATCACAT ACCGTGAATA TCTGGAGAAC ATGAATGATA AACGTCCGCC TCGCATTATC AAAACGATTG CATCTAAGAC TCAAAGCATT AAAAAATATA GCACCGACAT TTTGGGTAAC CTTTATGAAG TTAAATCTAA AAAGCACCCC CAGATTATCA AAAAAGGTAA GCGTCCAGCA GCCACAAAAA AAGCAGGCCA GGCGAAGAAA AAAAAACACC ACCACCACCA CCACTAGTGA GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGAT
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