pAC5.1b-EGFP
$128.00
In stock
SKU
PL82990
100μg liquid plasmid(Instantly):$149
| name | pAC5.1b-EGFP | another name | apAC5.1b-EGFP |
| Insert Size (bp) | Plasmid host | Insect cells | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | Ac5 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | Ac5-F：ACACAAAGCCGCTCCATCAG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTTCAAGGAA TTAATTCTAT ATTCTAAAAA CACAAATGAT ACTTCTAAAA AAAATCATGA ATGGCATCAA CTCTGAATCA AATCTTTGCA GATGCACCTA CTTCTCATTT CCACTGTCAC ATCATTTTTC CAGATCTCGC TGCCTGTTAT GTGGCCCACA AACCAAGACA CGTTTTATGG CCATTAAAGC TGGCTGATCG TCGCCAAACA CCAAATACAT ATCAATATGT ACATTCGAGA AAGAAGCGAT CAAAGAAGCG TCTTCGGGCG AGTAGGAGAA TGCGGAGGAG AAGGAGAACG AGCTGATCTA GTATCTCTCC ACAATCCAAT GCCAACTGAC CAACTGGCCA TATTCGGAGC AATTTGAAGC CAATTTCCAT CGCCTGGCGA TCGCTCCATT CTTGGCTATA TGTTTTTCAC CGTTCCCGGG GCCATTTTCA AAGACTCGTC GGTAAGATAA GATTGTGTCA CTCGCTGTCT CTCTTCATTT GTCGAAGAAT GCTGAGGAAT TTCGCGATGA CGTCGGCGAG TATTTTGAAG AATGAGAATA ATTTGTATTT ATACGAAAAT CAGTTAGTGG AATTTTCTAC AAAAACATGT TATCTATAGA TAATTTTGTT GCAAAATATG TTGACTATGA CAAAGATTGT ATGTATATAC CTTTAATGTA TTCTCATTTT CTTATGTATT TATAATGGCA ATGATGATAC TGATGATATT TTAAGATGAT GCCAGACCAC AGGCTGATTT CTGCGTCTTT TGCCGAACGC AGTGCATGTG CGGTTGTTGT TTTTTGGAAT AGTTTCAATT TTCGGACTGT CCGCTTTGAT TTCAGTTTCT TGGCTTATTC AAAAAGCAAA GTAAAGCCAA AAAAGCGAGA TGGCAATACC AAATGCGGCA AAACGGTAGT GGAAGGAAAG GGGTGCGGGG CAGCGGAAGG AAGGGTGGGG CGGGGCGTGG CGGGGTCTGT GGCTGGGCGC GACGTCACCG ACGTTGGAGC CACTCCTTTG ACCATGTGTG CGTGTGTGTA TTATTCGTGT CTCGCCACTC GCCGGTTGTT TTTTTCTTTT TATCTCGCTC TCTCTAGCGC CATCTCGTAC GCATGCTCAA CGCACCGCAT GTTGCCGTGT CCTTTATGCG TCATTTTGGC TCGAAATAGG CAATTATTTA AACAAAGATT AGTCAACGAA AACGCTAAAA TAAATAAGTC TACAATATGG TTACTTATTG CCATGTGTGT GCAGCCAACG ATAGCAACAA AAGCAACAAC ACAGTGGCTT TCCCTCTTTC ACTTTTTGTT TGCAAGCGCG TGCGAGCAAG ACGGCACGAC CGGCAAACGC AATTACGCTG ACAAAGAGCA GACGAAGTTT TGGCCGAAAA ACATCAAGGC GCCTGATACG AATGCATTTG CAATAACAAT TGCGATATTT AATATTGTTT ATGAAGCTGT TTGACTTCAA AACACACAAA AAAAAAAATA AAACAAATTA TTTGAAAGAG AATTAGGAAT CGGACAGCTT ATCGTTACGG GCTAACAGCA CACCGAGACG AAATAGCTTA CCTGACGTCA CAGCCTCTGG AAGAACTGCC GCCAAGCAGA GAGAGAGAGA AAAAGAGGGA GAGCAGCTTA GACCGCATGT GCTTGTGTGT GAGGCGTCTC TCTCTTCGTC TCCTGTTTGC GCAAACGCAT AGACTGCACT GAGAAAATCG ATTACCTATT TTTTATGAAT GAATATTTGC ACTATTACTA TTCAAAACTA TTAAGATAGC AATCACATTC AATAGCCAAA TACTATACCA CCTGAGCGAT GCAACGAAAT GATCAATTTG AGCAAAAATG CTGCATATTT AGGACGGCAT CATTATAGAA ATGCTTCTTG CTGTGTACTT TTCTCTCGTC TGGCAGCTGT TTCGCCGTTA TTGTTAAAAC CGGCTTAAGT TAGGTGTGTT TTCTACGACT AGTGATGCCC CTACTAGAAG ATGTGTGTTG CACAAATGTC CCTGAATAAC CAATTTGAAG TGCAGATAGC AGTAAACGTA AGCTAATATG AATATTATTT AACTGTAATG TTTTAATATC GCTGGACATT ACTAATAAAC CCACTATAAA CACATGTACA TATGTATGTT TTGGCATACA ATGAGTAGTT GGGGAAAAAA TGTGTAAAAG CACCGTGACC ATCACAGCAT AAAGATAACC AGCTGAAGTA TCGAATATGA GTAACCCCCA AATTGAATCA CATGCCGCAA CTGATAGGAC CCATGGAAGT ACACTCTTCA TGGCGATATA CAAGACACAC ACAAGCACGA ACACCCAGTT GCGGAGGAAA TTCTCCGTAA ATGAAAACCC AATCGGCGAA CAATTCATAC CCATATATGG TAAAAGTTTT GAACGCGACT TGAGAGCGGA GAGCATTGCG GCTGATAAGG TTTTAGCGCT AAGCGGGCTT TATAAAACGG GCTGCGGGAC CAGTTTTCAT ATCACTACCG TTTGAGTTCT TGTGCTGTGT GGATACTCCT CCCGACACAA AGCCGCTCCA TCAGCCAGCA GTCGTCTAAT CCAGAGACCC CGGATCGGGG TACCTACTAG TCCAGTGTGG TGGAATTTCG AGCTTTATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTTCTAGA GGGCCCGCGG TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAAGGCC TGAGCTCGCT GATCAGCCTC GATCGAGGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTATGGCTGA TTATGATCAG TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA CGATGATCGG CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG TCACTGGTCC CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC GGCATGGCGG CCGACGCGCT GGGCTACGTC TTGCTGGCGT TCGCGACGCG AGGCTGGATG GCCTTCCCCA TTATGATTCT TCTCGCTTCC GGCGGCATCG GGATGCCCGC GTTGCAGGCC ATGCTGTCCA GGCAGGTAGA TGACGACCAT CAGGGACAGC TTCAAGGATC GCTCGCGGCT CTTACCAGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GT
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