pBE2R
$84.00
In stock
SKU
PL82987
100μg liquid plasmid(Instantly):$120
| name | pBE2R | another name | apBE2R |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Kan | Fluorescent labeling | |
| Promoter | P43 | Replicon | repB |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | P43-F1：TGCCAAAGCTGTAATGGCTG | |
| 3′ sequencing primer | SP6：ATTTAGGTGACACTATAGAA | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTGTCACC TAAATCGTAT GTGTATGATA CATAAGGTTA TGTATTAATT GTAGCCGCGT TCTAACGACA ATATGTACAA GCCTAATTGT GTAGCATCTG GCTTACTGAA GCAGACCCTA TCATCTCTCT CGTAAACTGC CGTCAGAGTC GGTTTGGTTG GACGAACCTT CTGAGTTTCT GGTAACGCCG TTCCGCACCC CGGAAATGGT CAGCGAACCA ATCAGCAGGG TCATCGCTAG CCAGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC TGTTGGGCGC CATCTCCTTG CACCATTCCT TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC GCATAAGGGA GAGCGTCGAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGATTTCA CTTTTTGCAT TCTACAAACT GCATAACTCA TATGTAAATC GCTCCTTTTT AGGTGGCACA AATGTGAGGC ATTTTCGCTC TTTCCGGCAA CCACTTCCAA GTAAAGTATA ACACACTATA CTTTATATTC ATAAAGTGTG TGCTCTGCGA GGCTGTCGGC AGTGCCGACC AAAACCATAA AACCTTTAAG ACCTTTCTTT TTTTTACGAG AAAAAAGAAA CAAAAAAACC TGCCCTCTGC CACCTCAGCA AAGGGGGGTT TTGCTCTCGT GCTCGTTTAA AAATCAGCAA GGGACAGGTA GTATTTTTTG AGAAGATCAC TCAAAAAATC TCCACCTTTA AACCCTTGCC AATTTTTATT TTGTCCGTTT TGTCTAGCTT ACCGAAAGCC AGACTCAGCA AGAATAAAAT TTTTATTGTC TTTCGGTTTT CTAGTGTAAC GGACAAAACC ACTCAAAATA AAAAAGATAC AAGAGAGGTC TCTCGTATCT TTTATTCAGC AATCGCGCCC GATTGCTGAA CAGATTAATA ATAGATTTTA GCTTTTTATT TGTTGAAAAA AGCTAATCAA ATTGTTGTCG GGATCAATTA CTGCAAAGTC TCGTTCATCC CACCACTGAT CTTTTAATGA TGTATTGGGG TGCAAAATGC CCAAAGGCTT AATATGTTGA TATAATTCAT CAATTCCCTC TACTTCAATG CGGCAACTAG CAGTACCAGC AATAAACGAC TCCGCACCTG TACAAACCGG TGAATCATTA CTACGAGAGC GCCAGCTTCA TCACTTGCCT CCCATAGATG AATCCGAACC TCATTACACA TTAGAACTGC GAATCCATCT TCATGGTGAA CCAAAGTGAA ACCTAGTTTA TCGCAATAAA AACCTATACT CTTTTTAATA TCCCCGACTG GCAATGCCGG GATAGACTGT AACATTCTCA CGCATAAAAT CCCCTTTCAT TTTCTAATGT AAATCTATTA CCTTATTATT AATTCAATTC GCTCATAATT AATCCTTTTT CTTATTACGC AAAATGGCCC GATTTAAGCA CACCCTTTAT TCCGTTAATG CGCCATGACA GCCATGATAA TTACTAATAC TAGGAGAAGT TAATAAATAC GTAACCAACA TGATTAACAA TTATTAGAGG TCATCGTTCA AAATGGTATG CGTTTTGACA CATCCACTAT ATATCCGTGT CGTTCTGTCC ACTCCTGAAT CCCATTCCAG AAATTCTCTA GCGATTCCAG AAGTTTCTCA GAGTCGGAAA GTTGACCAGA CATTACGAAC TGGCACAGAT GGTCATAACC TGAAGGAAGA TCTGATTGCT TAACTGCTTC AGTTAAGACC GAAGCGCTCG TCGTATAACA GATGCGATGA TGCAGACCAA TCAACATGGC ACCTGCCATT GCTACCTGTA CAGTCAAGGA TGGTAGAAAT GTTGTCGGTC CTTGCACACG AATATTACGC CATTTGCCTG CATATTCAAA CAGCTCTTCT ACGATAAGGG CACAAATCGC ATCGTGGAAC GTTTGGGCTT CTACCGATTT AGCAGTTTGA TACACTTTCT CTAAGTATCC ACCTGAATCA TAAATCGGCA AAATAGAGAA AAATTGACCA TGTGTAAGCG GCCAATCTGA TTCCACCTGA GATGCATAAT CTAGTAGAAT CTCTTCGCTA TCAAAATTCA CTTCCACCTT CCACTCACCG GTTGTCCATT CATGGCTGAA CTCTGCTTCC TCTGTTGACA TGACACACAT CATCTCAATA TCCGAATAGG GCCCATCAGT CTGACGACCA AGAGAGCCAT AAACACCAAT AGCCTTAACA TCATCCCCAT ATTTATCCAA TATTCGTTCC TTAATTTCAT GAACAATCTT CATTCTTTCT TCTCTAGTCA TTATTATTGG TCCATTCACT ATTCTCATTC CCTTTTCAGA TAATTTTAGA TTTGCTTTTC TAAATAAGAA TATTTGGAGA GCACCGTTCT TATTCAGCTA TTAATAACTC GTCTTCCTAA GCATCCTTCA ATCCTTTTAA TAACAATTAT AGCATCTAAT CTTCAACAAA CTGGCCCGTT TGTTGAACTA CTCTTTAATA AAATAATTTT TCCGTTCCCA ATTCCACATT GCAATAATAG AAAATCCATC TTCATCGGCT TTTTCGTCAT CATCTGTATG AATCAAATCG CCTTCTTCTG TGTCATCAAG GTTTAATTTT TTATGTATTT CTTTTAACAA ACCACCATAG GAGATTAACC TTTTACGGTG TAAACCTTCC TCCAAATCAG ACAAACGTTT CAAATTCTTT TCTTCATCAT CGGTCATAAA ATCCGTATCC TTTACAGGAT ATTTTGCAGT TTCGTCAATT GCCGATTGTA TATCCGATTT ATATTTATTT TTCGGTCGAA TCATTTGAAC TTTTACATTT GGATCATAGT CTAATTTCAT TGCCTTTTTC CAAAATTGAA TCCATTGTTT TTGATTCACG TAGTTTTCTG TATTCTTAAA ATAAGTTGGT TCCACACATA CCAATACATG CATGTGCTGA TTATAAGAAT TATCTTTATT ATTTATTGTC ACTTCCGTTG CACGCATAAA ACCAACAAGA TTTTTATTAA TTTTTTTATA TTGCATCATT CGGCGAAATC CTTGAGCCAT ATCTGACAAA CTCTTATTTA ATTCTTCGCC ATCATAAACA TTTTTAACTG TTAATGTGAG AAACAACCAA CGAACTGTTG GCTTTTGTTT AATAACTTCA GCAACAACCT TTTGTGACTG AATGCCATGT TTCATTGCTC TCCTCCAGTT GCACATTGGA CAAAGCCTGG ATTTACAAAA CCACACTCGA TACAACTTTC TTTCGCCTGT TTCACGATTT TGTTTATACT CTAATATTTC AGCACAATCT TTTACTCTTT CAGCCTTTTT AAATTCAAGA ATATGCAGAA GTTCAAAGTA ATCAACATTA GCGATTTTCT TTTCTCTCCA TGGTCTCACT TTTCCACTTT TTGTCTTGTC CACTAAAACC CTTGATTTTT CATCTGAATA AATGCTACTA TTAGGACACA TAATATTAAA AGAAACCCCC ATCTATTTAG TTATTTGTTT AGTCACTTAT AACTTTAACA GATGGGGTTT TTCTGTGCAA CCAATTTTAA GGGTTTTCAA TACTTTAAAA CACATACATA CCAACACTTC AACGCACCTT TCAGCAACTA AAATAAAAAT GACGTTATTT CTATATGTAT CAAGATAAGA AAGAACAAGT TCAAAACCAT CAAAAAAAGA CACCTTTTCA GGTGCTTTTT TTATTTTATA AACTCATTCC CTGATCTCGA CTTCGTTCTT TTTTTACCTC TCGGTTATGA GTTAGTTCAA ATTCGTTCTT TTTAGGTTCT AAATCGTGTT TTTCTTGGAA TTGTGCTGTT TTATCCTTTA CCTTGTCTAC AAACCCCTTA AAAACGTTTT TAAAGGCTTT TAAGCCGTCT GTACGTTCCT TAAGGAATTC GAGCTCAGCT TTATTGAGTG GATGATTATA TTCCTTTTGA TAGGTGGTAT GTTTTCGCTT GAACTTTTAA ATACAGCCAT TGAACATACG GTTGATTTAA TAACTGACAA ACATCACCCT CTTGCTAAAG CGGCCAAGGA CGCTGCCGCC GGGGCTGTTT GCGTTTTTGC CGTGATTTCG TGTATTATTG GTTTACTTAT TTTTTTGCCA AAGCTGTAAT GGCTGAAAAT TCTTACATTT ATTTTACATT TTTAGAAATG GGCGTGAAAA AAAGCGCGCG ATTATGTAAA ATATAAAGTG ATAGCGGTAC CATTATAGGT AAGAGAGGAA TGTACACATG AAGAAACCGT TGGGGAAAAT TGTCGCAAGC ACCGCACTAC TCATTTCTGT TGCTTTTAGT TCATCGATCG CATCGGCTGC TGAAGAAGCA AAAGAAAAAT ATTTAATTGG CTTTAATGAG CAGGAAGCTG TCAGTGAGTT TGTAGAACAA GTAGAGGCAA ATGACGAGGT CGCCATTCTC TCTGAGGAAG AGGAAGTCGA AATTGAATTG CTTCATGAAT TTGAAACGAT TCCTGTTTTA TCCGTTGAGT TAAGCCCAGA AGATGTGGAC GCGCTTGAAC TCGATCCAGC GATTTCTTAT ATTGAAGAGG ATGCAGAAGT AACGACAATG GGATCCTCTA GAGTCGACCT GCAGCTCGAG CACCACCACC ACCACCACAA GCTTGTATTC TA
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