pBV220-EK
$128.00
In stock
SKU
PL82974
100μg liquid plasmid(Instantly):$149
| name | pBV220-EK | another name | apBV220-EK |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | protease | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | PL,PR | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | pBV220F：AAGAAGGGCAGCATTCAAAG | |
| 3′ sequencing primer | pBV220R：CTGCGTTCTGATTTAATCTG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACGTTAAATC TATCACCGCA AGGGATAAAT ATCTAACACC GTGCGTGTTG ACTATTTTAC CTCTGGCGGT GATAATGGTT GCATGTACTA AGGAGGTTGT ATGGAACAAC GCATAACCCT GAAAGATTAT GCAATGCGCT TTGGGCAAAC CAAGACAGCT AAAAGATCTC TCACCTACCA AACAATGCCC CCCTGCAAAA AATAAATTCA TATAAAAAAC ATACAGATAA CCATCTGCGG TGATAAATTA TCTCTGGCGG TGTTGACATA AATACCACTG GCGGTGATAC TGAGCACATC AGCAGGACGC ACTGACCACC ATGAAGGTGA CGCTCTTAAA AATTAAGCCC TGAAGAAGGG CAGCATTCAA AGCAGAAGGC TTTGGGGTGT GTGATACGAA ACGAAGCATT GGTTAAAAAT TAAGGAGGAA TTCATGCATC ATCATCATCA TCATATTGTC GGAGGAAGTG ACTGGCTGGT GTCCGCCGCA CACTGCGTGT ATGGGAGAAA CTTAGAGCCA TCCAAGTGGA CAGCAATCCT AGGCCTGCAT ATGAAATCAA ATCTGACCTC TCCTCAAACA GTCCCTCGAT CAATAGATGA AATTGTCATA AACCCTCATT ACAATAGGCG AAGAAAGGAC AACGACATTG CCATGATGCA TCTGGAATTT AAAGTGAATT ACACAGATTA CATACAACCT ATTTGTTTAC CGGAAGAAAA TCAAGTTTTT CCTCCAGGAA GAAATTGTTC TATTGCTGGT TGGGGGACGG TTGTATATCA AGGTACTACT GCAAACATAT TGCAAGAAGC TGATGTTCCT CTTCTATCAA ATGAGAGATG CCAACAGCAG ATGCCAGAAT ATAACATTAC TGAAAATATG ATATGTGCAG GCTATGAAGA AGGAGGAATA GATTCTTGTC AGGGGGATTC AGGAGGACCA TTAATGTGCC AAGAAAACAA CAGGTGGTTC CTTGCTGGTG TGACCTCATT TGGATACAAG TGTGCCCTGC CTAATCGCCC CGGAGTGTAT GCCAGGGTCT CAAGGTTTAC CGAGTGGATA CAAAGTTTTC TACATTAGGG ATCCGTCGAC CTGCAGCCAA GCTTCTGTTT TGGCTTATGA GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTC CCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGC CAACTGCCAG GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAAGGAA TCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CAACCTCTGA CTTGAGCGTC GATTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCTT ATCTTTCCCT TTATTTTTGC TGCGGTAAGT CGCATAAAAA CCATTCTTCA TAATTCAATC CATTTACTAT GTTATGTTCT GAGGGGAGTG AAAATTCCCC TAATTCGATG AAGATTCTTG CTCAATTGTT ATCAGCTATG CGCCGACCAG AACACCTTGC CGATCAGCCA AACGTCTCTT CAGGCCACTG ACTAGCGATA ACTTTCCCCA CAACGGAACA ACTCTCATTG CATGGGATCA TTGGGTACTG TGGGTTTAGT GGTTGTAAAA ACACCTGACC GCTATCCCTG ATCAGTTTCT TGAAGGTAAA CTCATCACCC CCAAGTCTGG CTATGCAGAA ATCACCTGGC TCAACAGCCT GCTCAGGGTC AACGAGAATT AACATTCCGT CAGGAAAGCT TGGCTTGGAG CCTGTTGGTG CGGTCATGGA ATTACCTTCA ACCTCAAGCC AGAATGCAGA ATCACTGGCT TTTTTGGTTG TGCTTACCCA TCTCTCCGCA TCACCTTTGG TAAAGGTTCT AAGCTTAGGT GAGAACATCC CTGCCTGAAC ATGAGAAAAA ACAGGGTACT CATACTCACT TCTAAGTGAC GGCTGCATAC TAACCGCTTC ATACATCTCG TAGATTTCTC TGGCGATTGA AGGGCTAAAT TCTTCAACGC TAACTTTGAG AATTTTTGCA AGCAATGCGG CGTTATAAGC ATTTAATGCA TTGATGCCAT TAAATAAAGC ACCAACGCCT GACTGCCCCA TCCCCATCTT GTCTGCGACA GATTCCTGGG ATAAGCCAAG TTCATTTTTC TTTTTTTCAT AAATTGCTTT AAGGCGACGT GCGTCCTCAA GCTGCTCTTG TGTTAATGGT TTCTTTTTTG TGCTCAT
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