pCRISPR-mCherry-Neo
$99.00
In stock
SKU
PL82972
pCRISPR-CG01
100μg liquid plasmid(Instantly):$120
| name | pCRISPR-mCherry-Neo | another name | apCRISPR-mCherry-Neo |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | Replicon | ||
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGACACAATT GCATGAAGAA TCTGCTTAGG GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTTG CCACCATGGA CAAGAAGTAC AGCATCGGCC TGGACATCGG TACCAACAGC GTGGGCTGGG CCGTGATCAC CGACGAGTAC AAGGTGCCCA GCAAGAAGTT CAAGGTGCTG GGCAACACCG ACCGCCACAG CATCAAGAAG AACCTGATCG GCGCCCTGCT GTTCGACAGC GGCGAGACCG CCGAGGCCAC CCGCCTGAAG CGCACCGCCC GCCGCCGCTA CACCCGCCGC AAGAACCGCA TCTGCTACCT GCAGGAGATC TTCAGCAACG AGATGGCCAA GGTGGACGAC AGCTTCTTCC ACCGCCTGGA GGAGAGCTTC CTGGTGGAGG AGGACAAGAA GCACGAGCGC CACCCCATCT TCGGCAACAT CGTGGACGAG GTGGCCTACC ACGAGAAGTA CCCCACCATC TACCACCTGC GCAAGAAGCT GGTGGACAGC ACCGACAAGG CCGACCTGCG CCTGATCTAC CTGGCCCTGG CCCACATGAT CAAGTTCCGC GGCCACTTCC TGATCGAGGG CGACCTGAAC CCCGACAACA GCGACGTGGA CAAGCTGTTC ATCCAGCTGG TGCAGACCTA CAACCAGCTG TTCGAGGAGA ACCCCATCAA CGCCAGCGGC GTGGACGCCA AGGCCATCCT GAGCGCCCGC CTGAGCAAGA GCCGCCGCCT GGAGAACCTG ATCGCCCAGC TGCCCGGCGA GAAGAAGAAC GGCCTGTTCG GCAACCTGAT CGCCCTGAGC CTGGGCCTGA CCCCCAACTT CAAGAGCAAC TTCGACCTGG CCGAGGACGC CAAGCTGCAG CTGAGCAAGG ACACCTACGA CGACGACCTG GACAACCTGC TGGCCCAGAT CGGCGACCAG TACGCCGACC TGTTCCTGGC CGCCAAGAAC CTGAGCGACG CCATCCTGCT GAGCGACATC CTGCGCGTGA ACACCGAGAT CACCAAGGCC CCCCTGAGCG CCAGCATGAT CAAGCGCTAC GACGAGCACC ACCAGGACCT GACCCTGCTG AAGGCCCTGG TGCGCCAGCA GCTGCCCGAG AAGTACAAGG AGATCTTCTT CGACCAGAGC AAGAACGGCT ACGCCGGCTA CATCGACGGC GGCGCCAGCC AGGAGGAGTT CTACAAGTTC ATCAAGCCCA TCCTGGAGAA GATGGACGGC ACCGAGGAGC TGCTGGTGAA GCTGAACCGC GAGGACCTGC TGCGCAAGCA GCGCACCTTC GACAACGGCA GCATCCCCCA CCAGATCCAC CTGGGCGAGC TGCACGCCAT CCTGCGCCGC CAGGAGGACT TCTACCCCTT CCTGAAGGAC AACCGCGAGA AGATCGAGAA GATCCTGACC TTCCGCATCC CCTACTACGT GGGCCCCCTG GCCCGCGGCA ACAGCCGCTT CGCCTGGATG ACCCGCAAGA GCGAGGAGAC CATCACCCCC TGGAACTTCG AGGAGGTGGT GGACAAGGGC GCCAGCGCCC AGAGCTTCAT CGAGCGCATG ACCAACTTCG ACAAGAACCT GCCCAACGAG AAGGTGCTGC CCAAGCACAG CCTGCTGTAC GAGTACTTCA CCGTGTACAA CGAGCTGACC AAGGTGAAGT ACGTGACCGA GGGCATGCGC AAGCCCGCCT TCCTGAGCGG CGAGCAGAAG AAGGCCATCG TGGACCTGCT GTTCAAGACC AACCGCAAGG TGACCGTGAA GCAGCTGAAG GAGGACTACT TCAAGAAGAT CGAGTGCTTC GACAGCGTGG AGATCAGCGG CGTGGAGGAC CGCTTCAACG CCAGCCTGGG CACCTACCAC GACCTGCTGA AGATCATCAA GGACAAGGAC TTCCTGGACA ACGAGGAGAA CGAGGACATC CTGGAGGACA TCGTGCTGAC CCTGACCCTG TTCGAGGACC GCGAGATGAT CGAGGAGCGC CTGAAGACCT ACGCCCACCT GTTCGACGAC AAGGTGATGA AGCAGCTGAA GCGCCGCCGC TACACCGGCT GGGGCCGCCT GAGCCGCAAG CTTATCAACG GCATCCGCGA CAAGCAGAGC GGCAAGACCA TCCTGGACTT CCTGAAGAGC GACGGCTTCG CCAACCGCAA CTTCATGCAG CTGATCCACG ACGACAGCCT GACCTTCAAG GAGGACATCC AGAAGGCCCA GGTGAGCGGC CAGGGCGACA GCCTGCACGA GCACATCGCC AACCTGGCCG GCAGCCCCGC CATCAAGAAG GGCATCCTGC AGACCGTGAA GGTGGTGGAC GAGCTGGTGA AGGTGATGGG CCGCCACAAG CCCGAGAACA TCGTGATCGA GATGGCCCGC GAGAACCAGA CCACCCAGAA GGGCCAGAAG AACAGCCGCG AGCGCATGAA GCGCATCGAG GAGGGCATCA AGGAGCTGGG CAGCCAGATC CTGAAGGAGC ACCCCGTGGA GAACACCCAG CTGCAGAACG AGAAGCTGTA CCTGTACTAC CTGCAGAACG GCCGCGACAT GTACGTGGAC CAGGAGCTGG ACATCAACCG CCTGAGCGAC TACGACGTGG ACCACATCGT GCCCCAGAGC TTCCTGAAGG ACGACAGCAT CGACAACAAG GTGCTGACCC GCAGCGACAA GAACCGCGGC AAGAGCGACA ACGTGCCCAG CGAGGAGGTG GTGAAGAAGA TGAAGAACTA CTGGCGCCAG CTGCTGAACG CCAAGCTGAT CACCCAGCGC AAGTTCGACA ACCTGACCAA GGCCGAGCGC GGCGGCCTGA GCGAGCTGGA CAAGGCCGGC TTCATCAAGC GCCAGCTGGT GGAGACCCGC CAGATCACCA AGCACGTGGC CCAGATCCTG GACAGCCGCA TGAACACCAA GTACGACGAG AACGACAAGC TGATCCGCGA GGTGAAGGTG ATCACCCTGA AGAGCAAGCT GGTGAGCGAC TTCCGCAAGG ACTTCCAGTT CTACAAGGTG CGCGAGATCA ACAACTACCA CCACGCCCAC GACGCCTACC TGAACGCCGT GGTGGGCACC GCCCTGATCA AGAAGTACCC CAAGCTGGAG AGCGAGTTCG TGTACGGCGA CTACAAGGTG TACGACGTGC GCAAGATGAT CGCCAAGAGC GAGCAGGAGA TCGGCAAGGC CACCGCCAAG TACTTCTTCT ACAGCAACAT CATGAACTTC TTCAAGACCG AGATCACCCT GGCCAACGGC GAGATCCGCA AGCGCCCCCT GATCGAGACC AACGGCGAGA CCGGCGAGAT CGTGTGGGAC AAGGGCCGCG ACTTCGCCAC CGTGCGCAAG GTGCTGAGCA TGCCCCAGGT GAACATCGTG AAGAAGACCG AGGTGCAGAC CGGCGGCTTC AGCAAGGAGA GCATCCTGCC CAAGCGCAAC AGCGACAAGC TGATCGCCCG CAAGAAGGAC TGGGACCCCA AGAAGTACGG CGGCTTCGAC AGCCCCACCG TGGCCTACAG CGTGCTGGTG GTGGCCAAGG TGGAGAAGGG CAAGAGCAAG AAGCTGAAGA GCGTGAAGGA GCTGCTGGGC ATCACCATCA TGGAGCGCAG CAGCTTCGAG AAGAACCCCA TCGACTTCCT GGAGGCCAAG GGCTACAAGG AGGTGAAGAA GGACCTGATC ATCAAGCTGC CCAAGTACAG CCTGTTCGAG CTGGAGAACG GCCGCAAGCG CATGCTGGCC AGCGCCGGCG AGCTGCAGAA GGGCAACGAG CTGGCCCTGC CCAGCAAGTA CGTGAACTTC CTGTACCTGG CCAGCCACTA CGAGAAGCTG AAGGGCAGCC CCGAGGACAA CGAGCAGAAG CAGCTGTTCG TGGAGCAGCA CAAGCACTAC CTGGACGAGA TCATCGAGCA GATCAGCGAG TTCAGCAAGC GCGTGATCCT GGCCGACGCC AACCTGGACA AGGTGCTGAG CGCCTACAAC AAGCACCGCG ACAAGCCCAT CCGCGAGCAG GCCGAGAACA TCATCCACCT GTTCACCCTG ACCAACCTGG GCGCCCCCGC CGCCTTCAAG TACTTCGACA CCACCATCGA CCGCAAGCGC TACACCAGCA CCAAGGAGGT GCTGGACGCC ACCCTGATCC ACCAGAGCAT CACCGGTCTG TACGAGACCC GCATCGACCT GAGCCAGCTG GGCGGCGACG GCGGCTCCGG ACCTCCAAAG AAAAAGAGAA AAGTATACCC CTACGACGTG CCCGACTACG CCTAATAACT CGAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCGGCGCGC CATACCGGTC GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGTAGGCG GCCGCGATCC CGCCCCTCTC CCTCCCCCCC CCCTAACGTT ACTGGCCGAA GCCGCTTGGA ATAAGGCCGG TGTGCGTTTG TCTATATGTT ATTTTCCACC ATATTGCCGT CTTTTGGCAA TGTGAGGGCC CGGAAACCTG GCCCTGTCTT CTTGACGAGC ATTCCTAGGG GTCTTTCCCC TCTCGCCAAA GGAATGCAAG GTCTGTTGAA TGTCGTGAAG GAAGCAGTTC CTCTGGAAGC TTCTTGAAGA CAAACAACGT CTGTAGCGAC CCTTTGCAGG CAGCGGAACC CCCCACCTGG CGACAGGTGC CTCTGCGGCC AAAAGCCACG TGTATAAGAT ACACCTGCAA AGGCGGCACA ACCCCAGTGC CACGTTGTGA GTTGGATAGT TGTGGAAAGA GTCAAATGGC TCACCTCAAG CGTATTCAAC AAGGGGCTGA AGGATGCCCA GAAGGTACCC CATTGTATGG GATCTGATCT GGGGCCTCGG TGCACATGCT TTACATGTGT TTAGTCGAGG TTAAAAAAAC GTCTAGGCCC CCCGAACCAC GGGGACGTGG TTTTCCTTTG AAAAACACGA TGATAAGCTT GCCACAACCC GCGATAATTC CTGCAGCCAA TATGGGATCG GCCATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCAGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG TATGCCCGAC GGCGATGATC TCGTCGTGAC TCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAACGCG GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGG TAAGCCTGAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT TTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC ATGATTACGA ATTGCAACGA TTTAGGTGAC ACTATAGAAG AGAAGGAATT AATACGACTC ACTATAGGGA GAGAGAGAGA ATTACCCTCA CTAAAGGGAG GAGAAGCATG AATTCCCCAG TGGAAAGACG CGCAGGCAAA ACGCACCACG TGACGGAGCG TGACCGCGCG CCGAGCGCGC GCCAAGGTCG GGCAGGAAGA GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGGTTTA TATATCTTGT GGAAAGGACG AGGATCCGGA GACGAAGCTT CGTCTCTGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTC T
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