pTol2-Promoter-T2A-EGFP
$128.00
In stock
SKU
PL82954
100μg liquid plasmid(Instantly):$149
| name | pTol2-Promoter-T2A-EGFP | another name | apTol2-Promoter-T2A-EGFP |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression,Transpose | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | ZBactin | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | Forward(left ITR)：CCTCTGACTTGAGCGTCGAT | |
| 3′ sequencing primer | pEGFP-N-3'：CGTCGCCGTCCAGCTCGACCAG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGAGGTGTA AAGTACTTGA GTAATTTTAC TTGATTACTG TACTTAAGTA TTATTTTTGG GGATTTTTAC TTTACTTGAG TACAATTAAA AATCAATACT TTTACTTTTA CTTAATTACA TTTTTTTAGA AAAAAAAGTA CTTTTTACTC CTTACAATTT TATTTACAGT CAAAAAGTAC TTATTTTTTG GAGATCACTT CATTCTATTT TCCCTTGCTA TTAGCTAGCG CTACCGGACT CAGATCTCGA GCGTTTACTC TGAGAAACTT CCATATTTTA GAGGTAAAAT GCTCAGAATT GTAAAAAGTC TTGTCAAAAT ACTTGGAATT TGCCAAAAAC AAAATAAAAG TCACATCACC TCATTGTCCT TGGTATCAGC AAGCACTTTT AACACAATCA GCTGTTTTTG TTTATAACTC CCACTGAAGG CCATGTGCAT TACTCCCAAG TTACACAGTA AAAAGTCACA TGTAGTTTAT CATGATCACT GCTGCTCAAG CACAGCTTTA TCTGTTATTC AGTGTGGCAA TATCTGAAAT GTGTGCAAAA TATTATTTAT TGTAGCTGAT CACCAACACT TTCAGTGTTT TTTTTTTCTA CTTTTAATGA GAATTATGTT CTTAGGATTT ATCCTTATAA ACCTCTTTAA AAAGAAATGT TATGTCTGCA TAGACACGAC ATGAAATTAT GAACAGAAAA TTTAACTATT TAAGATCAAG ACTTGACATA TAACATTTCT AATGAACATT AAACCTGAGG TAGGCCCACT TGCAAAAATG TAACTGCTAT CACTCACTTT ATACATCATT GTGTAACTTG TACACACAAA AATAGTGAAG CAATGTGGCA TGGAATGCAG GTCCCATTCA TTCAAAGTTA TTAAAGCACT TTCTTAAAGT GCATTACTAC TGAGCTAAGA CCAGCTAGTT GCTCATACAA CATACTACAA GTAGTGTCAT CCTAATTTGG ATAAAAAAAA TGTCCATTTT ATACATGGAA AAGTACTCAT TAGATCCCCC ATTGTTTGTA TTACGGTATT TCGTGAACAC AAGAGGTAAA TGACCTACAG AGCTGCTGTT GTGTTAGATT GAAAACACAA CACAGGATCA TGGAGGCATC CATCATTATG ACGCATTCTG CCACTTGTAA CTCTGCACAG ACTTTTTGAA AAGTTTAATT GAGTCTTGGC GTTGTCGCAG AAAAATACGA AAAATTCTGT GTTAAAATTT GCAAATAAAA ATTCCAGTTT TATAGGAAAT CGCCATGGCC TTGTTCTTTA ACCAGTTAAG CTTCCCCTTC TTTCACTCTC AAGTTGCAAG AAGCAAGTGT AGCAATGTGC ACGCGACAGC CGGGTGTGTG ACGCTGGACC AATCAGAGCA CAGAGCTCCG AAAGTTTACC TTTTATGGCT AGAGCCGGGT ATGTGCCGTC ATATAAAAGA GCGCGCCCAG CTTTTCAGCC TCACTTTGAG CTCCTCCACA CGCAGCTAGT GCGGAATATC ATCTGCTTGT AACCCATTCT CTTAAGTCGA CAACCCCCCA AACCCAAGGT GAGTTGGTTT TACAGTTTTT AAGCGTTTAA TTATAGTTTC TATCTATTAA TGTTAAGTAA AATGTGTAAA TGGATAAATA TGATTTGATG AAGTCTTTAG ACAGTTCATA ACGGTCTCTT TGTAGTTATC TACTAGGTTT GATTTATAAG TGGTGGTTCT TTTGATAATC TATGTTACAT TTAATAAATA TTGGCTGTAT CTTAACTTTT TTGTAGCGTT AGTGGATGTG GCAGGTGAGA ATGTCGGTAA TTTAACGTGA CCAGTCGTAG GCACGACATT GAACATTGAA TAGGCCGGTG TGAAATGGGT GTTAAGTCTT AATATAAAGT TGTGTTCTGG CTAATCTCTC TTTAACAGCC ATAAATGTGA TTTTTAACTA CAGTTTTGAG GGTGCTGGTG AGCATGTTGC ACACTTGATG GATAGCCGGC ATGGGAAGTT CTTTGTGCAG GCAGTGCTGC AGCAGGGTGT GACCTACTTT AGCTAGCCGG CTAACCAGCT CTCATCTGAT GTAACGTAAA CCCCATCAAT CTAACATCGG CTCATTCGTG CTTTATAAGA TCGAAGATCT TGTTATTAGT AACGGGTATT GGCATTTAGT TTTAACTTGG TTAATTTTCT TTTTGTTATG TGTTCTATTT AAATGTAGCC TGCAGCCCCC TCTGTCTTTA ATGAAGCTGA AGATTAACGT TAACGTTAGT GAAGTGTTGT ATCCGCTATG AAAGATTTGA ATTTTAGAGG TGTGGTCCAC TTTAATAACG GCTGCGGAGT TCTCTGGCTG CACTTCAGCC GCTGTGACTG TCGAAGCAGA GCCCATCAGT TTTTCAGGAT CTCGGTGATT TTTGAGGCTA GACTATGAAC TGAACCGACT GAGAGTGACC GTCCAGAAAT GCTTTAGTGT GAAGTCTCCG CTCACGAGGA GTCCGTGGAC TGACTGCAGA TCTGTGACGC AGTAACACCC GGCGGCAGAC ACCCGTTGAA ATGCGGTTGT GTAATTGATC GCAGGCGAGG GTGGAAGAGG ATGTGAAACT TCATTTGTGT AAAATTTAGG GAGTGGTCCT GGTGTGATGA ATGTCGAAAT CTGTTCCTTT TTACTGAGCC CTACGACTCT GGCTGAGTGC CACACCGCCG GCAGCCGCAA AGCGGCTCAA ACCATTGCCT TTTATGGTAA TAATGAGAGA ATGCAGAGGG ACTTCCTTTG TCTGGCATAT CTGAGGCGCG CGCTGTCACT AGCGCCCACC AGCGGTCAGA CTGTAGAATG CAGAGCAAAA CAGGAAGTTG ACTCCAGATG GTCACATGCT TGCTGAAACG CCTCTCCCTG GCAGCAGTGC AATTCTAACA GTTTCCCTTT CTTTTACAGT TCAGCCGAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA TCCGGAGGTG GCAGCGGTGG CGGTCAGCTG TTGAATTTTG ACCTTCTTAA ACTTGCGGGA GACGTCGAGT CCAACCCTGG GCCCACAACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGCG ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAGGCTT AAACAAGAAT CTCTAGTTTT CTTTCTTGCT TTTACTTTTA CTTCCTTAAT ACTCAAGTAC AATTTTAATG GAGTACTTTT TTACTTTTAC TCAAGTAAGA TTCTAGCCAG ATACTTTTAC TTTTAATTGA GTAAAATTTT CCCTAAGTAC TTGTACTTTC ACTTGAGTAA AATTTTTGAG TACTTTTTAC ACCTCTGCTT AAGGCGTAAA TTGTAAGCGT TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTCCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCTAGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCCTC AGGTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCATGC AT
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