pBARGPE1-mCherry
$157.00
In stock
SKU
PL82907
100μg liquid plasmid(Instantly):$178
| name | pBARGPE1-mCherry | another name | apBARGPE1-mCherry |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Bar | Fluorescent labeling | Red |
| Promoter | gpdA | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCCCCGTCCG GTCCTGCCCG TCACCGAGAT CCACTTAACG TTACTGAAAT CATCAAACAG CTTGACGAAT CTGGATATAA GATCGTTGGT GTCGATGTCA GCTCCGGAGT TGAGACAAAT GGTGTTCAGG ATCTCGATAA GATACGTTCA TTTGTCCAAG CAGCAAAGAG TGCCTTCTAG TGATTTAATA GCTCCATGTC AACAAGAATA AAACGCGTTT CGGGTTTACC TCTTCCAGAT ACAGCTCATC TGCAATGCAT GACGTTATTT ATGAGATGGG TTTTTATGAT TAGAGTCCCG CAATTATACA TTTAATACGC GATAGAAAAC AAAATATAGC GCGTGTACGC ATGTAACATT ATACTGAAAA CCTTGCTTGA GAAGGTTTTG GGACGCTCGA AGGCTTTAAT TTGCAAGCTT TGAAGAAAAA TGCGCCTTAT TCAATCTTTG CTATAAAAAA TGGCCCAAAA TCTCACATTG GAAGACATTT GATGACCTCA TTTCTTTCAA TGAAGGGCCT AACGGAGTTG ACTAATGTTG TGGGAAATTG GAGCGATAAG CGTGCTTCTG CCGTGGCCAG GACAACGTAT ACTCATCAGA TAACAGCAAT ACCTGATCGA CCAATTCCGA TCATATTCAA TAACCCTTAA TATAACTTCG TATAATGTAT GCTATACGAA GTTATTAGGT CTGAAGAGGA GTTTACGTCC AGCCAAGCTA GCTTGGCTGC AGGTCGAGCG GCCGCGATCC GGAACCCTTA ATATAACTTC GTATAATGTA TGCTATACGA AGTTATTAGG TCCCTCGACC GGCCGCGGCG GTTGTTTGCA AGACCGAGAA AAGGCTAGCA AGAATCGGGT CATTGTAGCG TATGCGCCTG TGAACATTCT CTTCAACAAG TTTGATTCCA TTGCGGTGAA ATGGTAAAAG TCAACCCCCT GCGATGTATA TTTTCCTGTA CAATCAATCA AAAAGCCAAA TGATTTAGCA TTATCTTTAC ATCTTGTTAT TTTACAGATT TTATGTTTAG ATCCACTAGT TTCTCGGTAC TATGCATTAC ATGTTAATTA TTACATGCTT AACGTAATTC AACAGAAATT ATATGATAAT CATCGCAAGA CCGGCAACAG GATTCAATCT TAAGAAACTT TATTGCCAAA TGTTTGAACG ATCTGCAGGT CGACGGATCA GATCTCGGTG ACGGGCAGGA CCGGACGGGG CGGTACCGGC AGGCTGAAGT CCAGCTGCCA GAAACCCACG TCATGCCAGT TCCCGTGCTT GAAGCCGGCC GCCCGCAGCA TGCCGCGGGG GGCATATCCG AGCGCCTCGT GCATGCGCAC GCTCGGGTCG TTGGGCAGCC CGATGACAGC GACCACGCTC TTGAAGCCCT GTGCCTCCAG GGACTTCAGC AGGTGGGTGT AGAGCGTGGA GCCCAGTCCC GTCCGCTGGT GGCGGGGGGA GACGTACACG GTCGACTCGG CCGTCCAGTC GTAGGCGTTG CGTGCCTTCC AGGGGCCCGC GTAGGCGATG CCGGCGACCT CGCCGTCCAC CTCGGCGACG AGCCAGGGAT AGCGCTCCCG CAGACGGACG AGGTCGTCCG TCCACTCCTG CGGTTCCTGC GGCTCGGTAC GGAAGTTGAC CGTGCTTGTC TCGATGTAGT GGTTGACGAT GGTGCAGACC GCCGGCATGT CCGCCTCGGT GGCACGGCGG ATGTCGGCCG GGCGTCGTTC TGGGCTCATG GTTACTTCCT AATCGAAGCT TTGCTTGGGT AGAATAGGTA AGTCAGATTG AATCTGAAAT AAAGGGAGGA AGGGCGAACT TAAGAAGGTA TGACCGGGTC GTTCACTTAC CTTGCTTGAC AAACGCACCA AGTTATCGTG CACCAAGCAG CAGATGATAA TAATGTCCTC GTTCCTGTCT GCTAATAAGA GTCACACTTC GAGCGCCGCC GCTACTGCTA CAAGTGGGGC TGATCTGACC AGTTGCCTAA ATGAACCATC TTGTCAAACG ACACAAATTT TGTGCTCACC GCCTGGACGA CTAAACCAAA ATAGGCATTC ATTGTTGACC TCCACTAGCT CCAGCCAAGC CCAAAAAGTG CTCCTTCAAT ATCATCTTCT GTCGAGTCTA GAGCACATTC TGCGGCCTCT GTGCTCTGCA AGCCGCAAAC TTTCACCAAT GGACCAGAAC TACCTGTGAA ATTAATAACA GACATACTCC AAGCTGCCTT TGTGTGCTTA ATCACGTATA CTCACGTGCT CAATAGTCAC CAATGCCCTC CCTCTTGGCC CTCTCCTTTT CTTTTTTCGA CCGAATTAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGAGATCT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCGGAGAGA CGGACGGACG CAGAGAGAAG GGCTGAGTAA TAAGCGCCAC TGCGCCAGAC AGCTCTGGCG GCTCTGAGGT GCAGTGGATG ATTATTAATC CGGGACCGGC CGCCCCTCCG CCCCGAAGTG GAAAGGCTGG TGTGCCCCTC GTTGACCAAG AATCTATTGC ATCATCGGAG AATATGGAGC TTCATCGAAT CACCGGCAGT AAGCGAAGGA GAATGTGAAG CCAGGGGTGT ATAGCCGTCG GCGAAATAGC ATGCCATTAA CCTAGGTACA GAAGTCCAAT TGCTTCCGAT CTGGTAAAAG ATTCACGAGA TAGTACCTTC TCCGAAGTAG GTAGAGCGAG TACCCGGCGC GTAAGCTCCC TAATTGGCCC ATCCGGCATC TGTAGGGCGT CCAAATATCG TGCCTCTCCT GCTTTGCCCG GTGTATGAAA CCGGAAAGGC CGCTCAGGAG CTGGCCAGCG GCGCAGACCG GGAACACAAG CTGGCAGTCG ACCCATCCGG TGCTCTGCAC TCGACCTGCT GAGGTCCCTC AGTCCCTGGT AGGCAGCTTT GCCCCGTCTG TCCGCCCGGT GTGTCGGCGG GGTTGACAAG GTCGTTGCGT CAGTCCAACA TTTGTTGCCA TATTTTCCTG CTCTCCCCAC CAGCTGCTCT TTTCTTTTCT CTTTCTTTTC CCATCTTCAG TATATTCATC TTCCCATCCA AGAACCTTTA ATCAAGCTTA TCGATACCGT CGACCTCGAC TCTAGAGGAT CCCCCGGGCT GCAGGAATTC GATATGGTGA GCAAGGGCGA GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA GTAAATCAAG CTTATCGATA CCGTCGACCT CGAGGGGGGG CCCGGTACC
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