pHiL-D2
$99.00
In stock
SKU
PL82903
100μg liquid plasmid(Instantly):$120
| name | pHiL-D2 | another name | apHiL-D2 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | HIS4 | Fluorescent labeling | |
| Promoter | AOX1 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 5'AOX：GACTGGTTCCAATTGACAAGC | |
| 3′ sequencing primer | 3'AOX：GCAAATGGCATTCTGACATCC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCGCGGC CGCGATCTAA CATCCAAAGA CGAAAGGTTG AATGAAACCT TTTTGCCATC CGACATCCAC AGGTCCATTC TCACACATAA GTGCCAAACG CAACAGGAGG GGATACACTA GCAGCAGACC GTTGCAAACG CAGGACCTCC ACTCCTCTTC TCCTCAACAC CCACTTTTGC CATCGAAAAA CCAGCCCAGT TATTGGGCTT GATTGGAGCT CGCTCATTCC AATTCCTTCT ATTAGGCTAC TAACACCATG ACTTTATTAG CCTGTCTATC CTGGCCCCCC TGGCGAGGTC ATGTTTGTTT ATTTCCGAAT GCAACAAGCT CCGCATTACA CCCGAACATC ACTCCAGATG AGGGCTTTCT GAGTGTGGGG TCAAATAGTT TCATGTTCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCG GTGGCACCTG TGCCGAAACG CAAATGGGGA AACAACCCGC TTTTTGGATG ATTATGCATT GTCCTCCACA TTGTATGCTT CCAAGATTCT GGTGGGAATA CTGCTGATAG CCTAACGTTC ATGATCAAAA TTTAACTGTT CTAACCCCTA CTTGACAGGC AATATATAAA CAGAAGGAAG CTGCCCTGTC TTAAACCTTT TTTTTTATCA TCATTATTAG CTTACTTTCA TAATTGCGAC TGGTTCCAAT TGACAAGCTT TTGATTTTAA CGACTTTTAA CGACAACTTG AGAAGATCAA AAAACAACTA ATTATTCGAA ACGAGGAATT CGCCTTAGAC ATGACTGTTC CTCAGTTCAA GTTGGGCACT TACGAGAAGA CCGGTCTTGC TAGATTCTAA TCAAGAGGAT GTCAGAATGC CATTTGCCTG AGAGATGCAG GCTTCATTTT TGATACTTTT TTATTTGTAA CCTATATAGT ATAGGATTTT TTTTGTCATT TTGTTTCTTC TCGTACGAGC TTGCTCCTGA TCAGCCTATC TCGCAGCTGA TGAATATCTT GTGGTAGGGG TTTGGGAAAA TCATTCGAGT TTGATGTTTT TCTTGGTATT TCCCACTCCT CTTCAGAGTA CAGAAGATTA AGTGAGAAGT TCGTTTGTGC AAGCTTATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC ATCGTCATCC TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT TATGCCGGTA CTGCCGGGCC TCTTGCGGGA TATCGTCCAT TCCGACAGCA TCGCCAGTCA CTATGGCGTG CTGCTAGCGC TATATGCGTT GATGCAATTT CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG GCCGCCGCCC AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG CGACCACACC CGTCCTGTGG ATCTATCGAA TCTAAATGTA AGTTAAAATC TCTAAATAAT TAAATAAGTC CCAGTTTCTC CATACGAACC TTAACAGCAT TGCGGTGAGC ATCTAGACCT TCAACAGCAG CCAGATCCAT CACTGCTTGG CCAATATGTT TCAGTCCCTC AGGAGTTACG TCTTGTGAAG TGATGAACTT CTGGAAGGTT GCAGTGTTAA CTCCGCTGTA TTGACGGGCA TATCCGTACG TTGGCAAAGT GTGGTTGGTA CCGGAGGAGT AATCTCCACA ACTCTCTGGA GAGTAGGCAC CAACAAACAC AGATCCAGCG TGTTGTACTT GATCAACATA AGAAGAAGCA TTCTCGATTT GCAGGATCAA GTGTTCAGGA GCGTACTGAT TGGACATTTC CAAAGCCTGC TCGTAGGTTG CAACCGATAG GGTTGTAGAG TGTGCAATAC ACTTGCGTAC AATTTCAACC CTTGGCAACT GCACAGCTTG GTTGTGAACA GCATCTTCAA TTCTGGCAAG CTCCTTGTCT GTCATATCGA CAGCCAACAG AATCACCTGG GAATCAATAC CATGTTCAGC TTGAGACAGA AGGTCTGAGG CAACGAAATC TGGATCAGCG TATTTATCAG CAATAACTAG AACTTCAGAA GGCCCAGCAG GCATGTCAAT ACTACACAGG GCTGATGTGT CATTTTGAAC CATCATCTTG GCAGCAGTAA CGAACTGGTT TCCTGGACCA AATATTTTGT CACACTTAGG AACAGTTTCT GTTCCGTAAG CCATAGCAGC TACTGCCTGG GCGCCTCCTG CTAGCACGAT ACACTTAGCA CCAACCTTGT GGGCAACGTA GATGACTTCT GGGGTAAGGG TACCATCCTT CTTAGGTGGA GATGCAAAAA CAATTTCTTT GCAACCAGCA ACTTTGGCAG GAACACCCAG CATCAGGGAA GTGGAAGGCA GAATTGCGGT TCCACCAGGA ATATAGAGGC CAACTTTCTC AATAGGTCTT GCAAAACGAG AGCAGACTAC ACCAGGGCAA GTCTCAACTT GCAACGTCTC CGTTAGTTGA GCTTCATGGA ATTTCCTGAC GTTATCTATA GAGAGATCAA TGGCTCTCTT AACGTTATCT GGCAATTGCA TAAGTTCCTC TGGGAAAGGA GCTTCTAACA CAGGTGTCTT CAAAGCGACT CCATCAAACT TGGCAGTTAG TTCTAAAAGG GCTTTGTCAC CATTTTGACG AACATTGTCG ACAATTGGTT TGACTAATTC CATAATCTGT TCCGTTTTCT GGATAGGACG ACGAAGGGCA TCTTCAATTT CTTGTGAGGA GGCCTTAGAA ACGTCAATTT TGCACAATTC AATACGACCT TCAGAAGGGA CTTCTTTAGG TTTGGATTCT TCTTTAGGTT GTTCCTTGGT GTATCCTGGC TTGGCATCTC CTTTCCTTCT AGTGACCTTT AGGGACTTCA TATCCAGGTT TCTCTCCACC TCGTCCAACG TCACACCGTA CTTGGCACAT CTAACTAATG CAAAATAAAA TAAGTCAGCA CATTCCCAGG CTATATCTTC CTTGGATTTA GCTTCTGCAA GTTCATCAGC TTCCTCCCTA ATTTTAGCGT TCAACAAAAC TTCGTCGTCA AATAACCGTT TGGTATAAGA ACCTTCTGGA GCATTGCTCT TACGATCCCA CAAGGTGGCT TCCATGGCTC TAAGACCCTT TGATTGGCCA AAACAGGAAG TGCGTTCCAA GTGACAGAAA CCAACACCTG TTTGTTCAAC CACAAATTTC AAGCAGTCTC CATCACAATC CAATTCGATA CCCAGCAACT TTTGAGTTGC TCCAGATGTA GCACCTTTAT ACCACAAACC GTGACGACGA GATTGGTAGA CTCCAGTTTG TGTCCTTATA GCCTCCGGAA TAGACTTTTT GGACGAGTAC ACCAGGCCCA ACGAGTAATT AGAAGAGTCA GCCACCAAAG TAGTGAATAG ACCATCGGGG CGGTCAGTAG TCAAAGACGC CAACAAAATT TCACTGACAG GGAACTTTTT GACATCTTCA GAAAGTTCGT ATTCAGTAGT CAATTGCCGA GCATCAATAA TGGGGATTAT ACCAGAAGCA ACAGTGGAAG TCACATCTAC CAACTTTGCG GTCTCAGAAA AAGCATAAAC AGTTCTACTA CCGCCATTAG TGAAACTTTT CAAATCGCCC AGTGGAGAAG AAAAAGGCAC AGCGATACTA GCATTAGCGG GCAAGGATGC AACTTTATCA ACCAGGGTCC TATAGATAAC CCTAGCGCCT GGGATCATCC TTTGGACAAC TCTTTCTGCC AAATCTAGGT CCAAAATCAC TTCATTGATA CCATTATTGT ACAACTTGAG CAAGTTGTCG ATCAGCTCCT CAAATTGGTC CTCTGTAACG GATGACTCAA CTTGCACATT AACTTGAAGC TCAGTCGATT GAGTGAACTT GATCAGGTTG TGCAGCTGGT CAGCAGCATA GGGAAACACG GCTTTTCCTA CCAAACTCAA GGAATTATCA AACTCTGCAA CACTTGCGTA TGCAGGTAGC AAGGGAAATG TCATACTTGA AGTCGGACAG TGAGTGTAGT CTTGAGAAAT TCTGAAGCCG TATTTTTATT ATCAGTGAGT CAGTCATCAG GAGATCCTCT ACGCCGGACG CATCGTGGCC GGCATCACCG GCGCCACAGG TGCGGTTGCT GGCGCCTATA TCGCCGACAT CACCGATGGG GAAGATCGGG CTCGCCACTT CGGGCTCATG AGCGCTTGTT TCGGCGTGGG TATGGTGGCA GGCCCCGTGG CCGGGGGACT GTTGGGCGCC ATCTCCTTGC ATGCACCATT CCTTGCGGCG GCGGTGCTCA ACGGCCTCAA CCTACTACTG GGCTGCTTCC TAATGCAGGA GTCGCATAAG GGAGAGCGTC GAGTATCTAT GATTGGAAGT ATGGGAATGG TGATACCCGC ATTCTTCAGT GTCTTGAGGT CTCCTATCAG ATTATGCCCA ACTAAAGCAA CCGGAGGAGG AGATTTCATG GTAAATTTCT CTGACTTTTG GTCATCAGTA GACTCGAACT GTGAGACTAT CTCGGTTATG ACAGCAGAAA TGTCCTTCTT GGAGACAGTA AATGAAGTCC CACCAATAAA GAAATCCTTG TTATCAGGAA CAAACTTCTT GTTTCGAACT TTTTCGGTGC CTTGAACTAT AAAATGTAGA GTGGATATGT CGGGTAGGAA TGGAGCGGGC AAATGCTTAC CTTCTGGACC TTCAAGAGGT ATGTAGGGTT TGTAGATACT GATGCCAACT TCAGTGACAA CGTTGCTATT TCGTTCAAAC CATTCCGAAT CCAGAGAAAT CAAAGTTGTT TGTCTACTAT TGATCCAAGC CAGTGCGGTC TTGAAACTGA CAATAGTGTG CTCGTGTTTT GAGGTCATCT TTGTATGAAT AAATCTAGTC TTTGATCTAA ATAATCTTGA CGAGCCAAGG CGATAAATAC CCAAATCTAA AACTCTTTTA AAACGTTAAA AGGACAAGTA TGTCTGCCTG TATTAAACCC CAAATCAGCT CGTAGTCTGA TCCTCATCAA CTTGAGGGGC ACTATCTTGT TTTAGAGAAA TTTGCGGAGA TGCGATATCG AGAAAAAGGT ACGCTGATTT TAAACGTGAA ATTTATCTCA AGATCGCGGC CGCGATCTCG AATAATAACT GTTATTTTTC AGTGTTCCCG ATCTGCGTCT ATTTCACAAT ACCAACATGA GTCAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTAA TTCGATGATA AGCTGTCAAA CATGAGAAAT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA ATTGTAAACG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCATTG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG
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