pIB139
$128.00
In stock
SKU
PL82902
100μg liquid plasmid(Instantly):$149
| name | pIB139 | another name | apIB139 |
| Insert Size (bp) | Plasmid host | Streptococcus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Apr | |
| Selective marker | Fluorescent labeling | ||
| Promoter | ermEp | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | phage-F：TGATCATGTTGAGCCGCC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCCGGCAGGG AAACGTCATG GACCTGATTC ACCTGATTAT GCGGCTCGAC GCGTCGCACA AAGAATCTTC GCTGAAGTCG GCGAAGATTC TCGACACGAA GAACCTTCAG CGCGAATTGG GCGGGTACGT CGGCGGGAAG GCGCCTTACG GCTTCGAGCT TGTTTCGGAG ACGAAGGAGA TCACGCGCAA CGGCCGAATG GTCAATGTCG TCATCAACAA GCTTGCGCAC TCGACCACTC CCCTTACCGG ACCCTTCGAG TTCGAGCCCG ACGTAATCCG GTGGTGGTGG CGTGAGATCA AGACGCACAA ACACCTTCCC TTCAAGCCGG GCAGTCAAGC CGCCATTCAC CCGGGCAGCA TCACGGGGCT TTGTAAGCGC ATGGACGCTG ACGCCGTGCC GACCCGGGGC GAGACGATTG GGAAGAAGAC CGCTTCAAGC GCCTGGGACC CGGCAACCGT TATGCGAATC CTTCGGGACC CGCGTATTGC GGGCTTCGCC GCTGAGGTGA TCTACAAGAA GAAGCCGGAC GGCACGCCGA CCACGAAGAT TGAGGGTTAC CGCATTCAGC GCGACCCGAT CACGCTCCGG CCGGTCGAGC TTGATTGCGG ACCGATCATC GAGCCCGCTG AGTGGTATGA GCTTCAGGCG TGGTTGGACG GCAGGGGGCG CGGCAAGGGG CTTTCCCGGG GGCAAGCCAT TCTGTCCGCC ATGGACAAGC TGTACTGCGA GTGTGGCGCC GTCATGACTT CGAAGCGCGG GGAAGAATCG ATCAAGGACT CTTACCGCTG CCGTCGCCGG AAGGTGGTCG ACCCGTCCGC ACCTGGGCAG CACGAAGGCA CGTGCAACGT CAGCATGGCG GCACTCGACA AGTTCGTTGC GGAACGCATC TTCAACAAGA TCAGGCACGC CGAAGGCGAC GAAGAGACGT TGGCGCTTCT GTGGGAAGCC GCCCGACGCT TCGGCAAGCT CACTGAGGCG CCTGAGAAGA GCGGCGAACG GGCGAACCTT GTTGCGGAGC GCGCCGACGC CCTGAACGCC CTTGAAGAGC TGTACGAAGA CCGCGCGGCA GGCGCGTACG ACGGACCCGT TGGCAGGAAG CACTTCCGGA AGCAACAGGC AGCGCTGACG CTCCGGCAGC AAGGGGCGGA AGAGCGGCTT GCCGAACTTG AAGCCGCCGA AGCCCCGAAG CTTCCCCTTG ACCAATGGTT CCCCGAAGAC GCCGACGCTG ACCCGACCGG CCCTAAGTCG TGGTGGGGGC GCGCGTCAGT AGACGACAAG CGCGTGTTCG TCGGGCTCTT CGTAGACAAG ATCGTTGTCA CGAAGTCGAC TACGGGCAGG GGGCAGGGAA CGCCCATCGA GAAGCGCGCT TCGATCACGT GGGCGAAGCC GCCGACCGAC GACGACGAAG ACGACGCCCA GGACGGCACG GAAGACGTAG CGGCGTAGCG AGACACCCGG GAAGCCTGAT CTACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTTATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTTGGGC TGCAGGTCGA CTCTAGTATG CATGCGAGTG TCCGTTCGAG TGGCGGCTTG CGCCCGATGC TAGTCGCGGT TGATCGGCGA TCGCAGGTGC ACGCGGTCGA TCTTGACGGC TGGCGAGAGG TGCGGGGAGG ATCTGACCGA CGCGGTCCAC ACGTGGCACC GCGATGCTGT TGTGGGCACA ATCGTGCCGG TTGGTAGGAT CCACATATGT TGGGGATCCT CTAGAGGATC CGCGGCCGCG CGCGATATCG AATTCGTAAT CATGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTGGTTCA TGTGCAGCTC CATCAGCAAA AGGGGATGAT AAGTTTATCA CCACCGACTA TTTGCAACAG TGCCGTTGAT CGTGCTATGA TCGACTGATG TCATCAGCGG TGGAGTGCAA TGTCGTGCAA TACGAATGGC GAAAAGCCGA GCTCATCGGT CAGCTTCTCA ACCTTGGGGT TACCCCCGGC GGTGTGCTGC TGGTCCACAG CTCCTTCCGT AGCGTCCGGC CCCTCGAAGA TGGGCCACTT GGACTGATCG AGGCCCTGCG TGCTGCGCTG GGTCCGGGAG GGACGCTCGT CATGCCCTCG TGGTCAGGTC TGGACGACGA GCCGTTCGAT CCTGCCACGT CGCCCGTTAC ACCGGACCTT GGAGTTGTCT CTGACACATT CTGGCGCCTG CCAAATGTAA AGCGCAGCGC CCATCCATTT GCCTTTGCGG CAGCGGGGCC ACAGGCAGAG CAGATCATCT CTGATCCATT GCCCCTGCCA CCTCACTCGC CTGCAAGCCC GGTCGCCCGT GTCCATGAAC TCGATGGGCA GGTACTTCTC CTCGGCGTGG GACACGATGC CAACACGACG CTGCATCTTG CCGAGTTGAT GGCAAAGGTT CCCTATGGGG TGCCGAGACA CTGCACCATT CTTCAGGATG GCAAGTTGGT ACGCGTCGAT TATCTCGAGA ATGACCACTG CTGTGAGCGC TTTGCCTTGG CGGACAGGTG GCTCAAGGAG AAGAGCCTTC AGAAGGAAGG TCCAGTCGGT CATGCCTTTG CTCGGTTGAT CCGCTCCCGC GACATTGTGG CGACAGCCCT GGGTCAACTG GGCCGAGATC CGTTGATCTT CCTGCATCCG CCAGAGGCGG GATGCGAAGA ATGCGATGCC GCTCGCCAGT CGATTGGCTG AGCTCATGAG CGGAGAACGA GATGACGTTG GAGGGGCAAG GTCGCGCTGA TTGCTGGGGC AACACGTGGA GCGGATCGGG GATTGTCTTT CTTCAGCTCG CTGATGATAT GCTGACGCTC AATGCCGTTT GGCCTCCGAC TAACGAAAAT CCCGCATTTG GACGGCTGAT CCGATTGGCA CGGCGGACGG CGAATGGCGG AGCAGACGCT CGTCCGGGGG CAATGAGATA TGAAAAAGCC TGAACTCACC GCGACGTATC GGGCCCTGGC CAGCTAGCTA GAGTCGACCT GCAGGTCCCC GGGGATCGGT CTTGCCTTGC TCGTCGGTGA TGTACTTCAC CAGCTCCGCG AAGTCGCTCT TCTTGATGGA GCGCATGGGG ACGTGCTTGG CAATCACGCG CACCCCCCGG CCGTTTTAGC GGCTAAAAAA GTCATGGCTC TGCCCTCGGG CGGACCACGC CCATCATGAC CTTGCCAAGC TCGTCCTGCT TCTCTTCGAT CTTCGCCAGC AGGGCGAGGA TCGTGGCATC ACCGAACCGC GCCGTGCGCG GGTCGTCGGT GAGCCAGAGT TTCAGCAGGC CGCCCAGGCG GCCCAGGTCG CCATTGATGC GGGCCAGCTC GCGGACGTGC TCATAGTCCA CGACGCCCGT GATTTTGTAG CCCTGGCCGA CGGCCAGCAG GTAGGCCGAC AGGCTCATGC CGGCCGCCGC CGCCTTTTCC TCAATCGCTC TTCGTTCGTC TGGAAGGCAG TACACCTTGA TAGGTGGGCT GCCCTTCCTG GTTGGCTTGG TTTCATCAGC CATCCGCTTG CCCTCATCTG TTACGCCGGC GGTAGCCGGC CAGCCTCGCA GAGCAGGATT CCCGTTGAGC ACCGCCAGGT GCGAATAAGG GACAGTGAAG AAGGAACACC CGCTCGCGGG TGGGCCTACT TCACCTATCC TGCCCGGCTG ACGCCGTTGG ATACACCAAG GAAAGTCTAC ACGAACCCTT TGGCAAAATC CTGTATATCG TGCGAAAAAG GATGGATATA CCGAAAAAAT CGCTATAATG ACCCCGAAGC AGGGTTATGC AGCGGAAAAG ATCCGTCGAC CTGCAGGCAT GCAAGCTCTA GCGATTCCAG ACGTCCCGAA GGCGTGGCGC GGCTTCCCCG TGCCGGAGCA ATCGCCCTGG GTGGGTTACA CGACGCCCCT CTATGGCCCG TACTGACGGA CACACCGAAG CCCCGGCGGC AACCCTCAGC GGATGCCCCG GGGCTTCACG TTTTCCCAGG TCAGAAGCGG TTTTCGGGAG TAGTGCCCCA ACTGGGGTAA CCTTTGAGTT CTCTCAGTTG GGGGCGTAGG GTCGCCGACA TGACACAAGG GGTTGTGACC GGGGTGGACA CGTACGCGGG TGCTTACGAC CGTCAGTCGC GCGAGCGCGA AAATTCGAGC GCAGCAAGCC CAGCGACACA GCGTAGCGCC AACGAAGACA AGGCGGCCGA CCTTCAGCGC GAAGTCGAGC GCGACGGGGG CCGGTTCAGG TTCGTCGGGC ATTTCAGCGA AGCGCCGGGC ACGTCGGCGT TCGGGACGGC GGAGCGCCCG GAGTTCGAAC GCATCCTGAA CGAATGCCGC GCCGGGCGGC TCAACATGAT CATTGTCTAT GACGTGTCGC GCTTCTCGCG CCTGAAGGTC ATGGACGCGA TTCCGATTGT CTCGGAATTG CTCGCCCTGG GCGTGACGAT TGTTTCCACT CAGGAAGGCG TCT
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