pRADK
$128.00
In stock
SKU
PL82898
100μg liquid plasmid(Instantly):$149
| name | pRADK | another name | apRADK |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | GroEL | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | repU-F：GTAACCATGCGCCTAAACCT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC GCGAGGCCTC GAGATCTATC GATTCACAAA AAATAGGCAC ACGAAAAACA AGTTAAGGGA TGCAGTTTAT GCATGCCATG GTACCCGGGA GCTCGAATTC TAGAAGCTTC GTATTGTCGC CCTACATATA TACGTTAAAG CTAACAGCTG GCAAGGGGAT ACCCCCATTC CCCGTCCCAG CGCCCCTTGA GCGTCATAGA CTCAGATTGT CAGCTTCGGT CAGTTGACAT TTTTCTTATC GGCGCTCTAC CATCCGTGAC GGATTGAAGG CGCTGGGCGG GAAAAAGCTC GCCGGCACGA CTCTCCGCCA TTCCATCTCA CTCACAGGAG GACCCCATAT GAGCCATATT CAACGGGAAA CGTCTTGCTC TAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA TCGATTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGGAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AGTGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAGGATCCG ATTCGACCTG CAGGCATGCA AGCTGTACGC CTCAGCTACC ATTCTTGCAG CCGCGTTCAG GGTCTTGACG TGTTCTTTGT ATCTATCAGC AAACTGAGAA CCCAATTCTG GATCATCCTT GTAACGCCAA CTCTCCGACC ATTCCGAAGG AACGCCTTGT AGTCCTCCTG AGCCGTTAGG GTATGTCTTT AGTCCAATCA AATGGGACAT TTGCAGACAG GAACGCACAA GTGTCTCACG TCGCTTCTCT ATTTCAATAT CTAAAAATTC TTCACTCTTA TCGTTGCTTG ACATATTAAA ACTGTCTAAC TGATCCAGTT CACTTATATG GAAAGTATCA CTAAAGTCAT GGCTTCTCAG AAATTCAATA CTTCCCATTG ATGGGGACCG GTTAACCAAA TCGATAAAAA TTTTTCTGTC ATGTTTCAGA TAAGTTTTTG GCGCGTTGAT TTTATAATAA AGCCGCCATT CTTGTGCCAA CAACGCCACG AAAAGAGCCA CAGCAGCAGC GGCCACATCC CATTGAAAAT CTTTCGCCAG ACCAACCGCC AGGAGAACCA AGCCGAAATA GTTAGCCCAG CGAACTAAAA AACTATCTAT AAACGTATTA AGAAACGTTC GGGTCATATT TCCGTGAATC ATATCAGGAC GTAAATTTGC ACATGACGCA TTTCCGGGGG CTTTTATCCC TCGGACTTCT TTTCAGGCCC CTTCTCGGCC TTTCTGTGAC CCTGTTTTTT CAGGCCGCGA ACTGCGCCTG GTCGGGCCTG ACCCCCACAC GCTGGCCTTC GTACTCACGC AGCCTGCCCA GCAGGTCGGC CAGCGCGGCA TTGACCACGG CGGCGAGGTT GCGGGGCGGC GTTCCGCCTG CGGTCTGGTC GTGCTTCACG TCGCGCAGCA CGCGCACCAG CACCGCGCCC ACGTCGTCGG ACACGTCCCG GCCAGCGTCG GCGGCGCGGG TGATGTTCCA AATGAGTTTC CGCCAGAAGC CCAGCGAGTC GGCCCCGTCC CCGAAAGCTG CCGCTAGCGC CCGCGCCTGC CTGTCCACGA TCTCGGCGCG GTAGGCCGGG CGGGTTTCCC GGTGGGCGTC GGCAAGCTCC CACACGACGT TTTCAGCGAG GGAGGGCGCG GGCCGGACAG TCATGTTATC GGAGGGGGAG GGAAGAGCGG ATTTTATCGC CCAGGTGCTC ACAACTTCGT ATGTCACGGC AACCGGGGCG CTTTTTTCGC GCAGTTCCCC GCCTGTCACA TCCTCACAGG GTTCTTGACT CTCCGGCACG CTCTTAGGGA GAGGGTGGAG CATGTTGTAG ACGGTGCGCC CGGCCTTCGC GTCGGCGTTC AGGTCGCGCC AGTTCCGGCC CCAATCGTCA TGCATCAGGC GGACATATCC CGCCTTCCCT TCCAGCACGC GCCTCGGCTT GAGCGTCACG GCCCACAGCG TCCCGGTTGC CACAGACTCG CCGCGCAGGT CGCCCATGTG GGCATCACAG GCCACCAGCC CGACCCGGCG CAGATATTGA AGGTTCTCGT AGAAGGCGGA CTTCTTCAGA CCCACATGCA CCATGAGCAG CTCGGCGGGC AGGTGGAACA CAGCGCGGGT GACGTGTTCC GCATAGCCGC AGGCGCGGGC CACGTCCAGC GCCACCGAGA ACAGGGCGCG GAAGATGCGC CGGGCCGACT CGCGGCAGGG GGCGTCATCT AGGCAGGCGG TGAGGGTCTG CACCAGTTCG GCGCGTGTGG CGATGGTCAA CGGCGCGGGC GCGGGCTGGG ACTGCTGGGC GATGGTGGGC ACGGGCGGCG GCGAGATGGA CCGGGCCGAG CTGGAAATTT CCCGTGTGAG CGCAGGTTTA GGCGCATGGT TACCTTCCTG CAGTGAAATC TGCGAAATCA GGCCCGCTTC GAGGAGTCGG ACCATGAATG GATTGTGTCT CATGAAAAAA GCCTCCCCTC TTGTCAGAGG GGAGGGTCCC GGTCTACCAT GCTCTCAGCG ACGAGATAGC TGGGGTTTGT GACCCTGCAC TCTGACAAAG CCCTCACCGA AAGGTGGGGG TTTAGTCATT TGGCCTAGTA AAGCAGAACA GAGGCGATTT GTCACGCAAA GGCCCGCCGC CCGAGGGGAA ACAGGCGGCG GGCCAATGGC TCGGCGTTGT TAGCTGGGGG TCGCACCAGG GAAGCACAGA AGCCCTTCCC TGAGCAGGGT TTCACCGTGT GGTGTCCCTT CGTCCTTGAA GGCGAACTGG TAGGCCAGGC CGTAATGCTC CTGCACCATC TTTTGCTCAT CCACCGCGAC AAAGAAACGG CGGGTGTTCG CCGGGCTGCT GAGGGTCTGC CACTCGCCGG ACGCGCCCAC CGCATAGGCG TCTCCGGCTC CACATTCCTC GGCCTGCTGA TACTGATACT TCGTCCACAG CATCACCGCC TTATCGGGCA CCTGCGCGAA GCTCACCAGC GCCCCGAACC GCCCGGCGCT CGTGGTGAAG GTGGACGACT GCACCATGTC GGACAGTTCC AGCGTGGCAG GCGCGGGCGT GTTCGTCGGC GCGTACACCG TCCCCGCCTC GCACACGACC AGCGGGGCGG CCCCCGTGCC GAACTCGGCG GCGCTGCCGT TTGCCAGCGT GAGCGCCGAA GACGCCACCG CGCAGGTACC CATGGGTGCC GACCACCAGC AGGTCCACGC CGTGCGCCGC CTCGGTGAGC ACCTCGATTG GACGGCCCCT CAGGGTCTGA ACCCTCCCCT GCACGCCCGC CTCCCCGATG AGTGTCTGCG CCGTCTGCTG CGCCTGCCGG GCATTCTGCT CCAGCATCTC CCGGACGTAG TCGGGATTCA GTCCCGCCCA CTGAACGAAG CCGCTGACCG GCGGCTCCTC GATCACAGTC ACGACCTCCA GCTCGGCGCC TGCCGCCTTC CCGAGCGTTA TGGCCTGCTT CAAGGCGCGC TGGCTGGGCT CACTTCCGTC AAAGGCCACC ACGAATCGCA TCCGTCCTCC TCCCTGACCA GTCAGGTTCA GCAAGCAGGG TAAGCCTCTC CCAGCACTCC GGGGCTTCAC ACGGCCTTGG GTCTTTTCTG CATACCCATG AGCCTCAGTA CCCGACAGTT TCCGGCAGGG CTTGAAAAAG TTCGGCTGAC ACGCGAGAAC TGGACGCGTC TCCCGATGTC CTGGCCAAGT TGACCCGCTG CTTCACCGCG CACTTCCCGG AGTTCCGCAA GAACTAGGTC GAGCTGCTCT CCCTCATGGT CCTCGCCCTC CTTAGGGGCA AGGACGTCCG GCATGCTAAA CTCGCCGCGC GCTTCCCCGG AAGCGCGCAC ACCGCCTCCG TCATCCGGCG GGTGGAACGC TTCTTCGACC GTCATCCTCT TCGGCCAGCT GATGTCGCCC GGGTCGTTCT GACGCTCCTT CCCGCCGCGC AGCCACGCGA ATTTATCCTT GACCGGACCA ACTGGAAGTA TGGGCAGACG GACGTGAACG TCTTGTTGCT GGCCGTCATT TGGCGGGACG TCGCCATCCC CCTGCTCTAC GAGTTGCTGC CCCATGGGGG CAGCAGCGAC ACCGAGATTC GGCACACCCT GATGGACGAT GCCCTGTGCC TGCTGTCCGC CGCTGACATC CGGGTGCTGT ATTAGCCGAC CGCGAATTCC CAGCTTGAGT AGGACAAATC CGCCGAGCTT CGACGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATCG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTCTGT GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AATTTTTTTA AGGCAGTTAT TGGTGCCCTT AAACGCCTGG TGCTACGCCT GAATAAGTGA TAATAAGCGG ATGAATGGCA GAAATTCGTC GAGGCGGCAC CTCGCTAACG GATTCACCAC TCCAAGAATT GGAGCCAATC AATTCTTGCG GAGAACTGTG AATGCATCCC GCAAGAGGCC CGGCAGT
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