pKC1139
$128.00
In stock
SKU
PL82875
100μg liquid plasmid(Instantly):$149
| name | pKC1139 | another name | apKC1139 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Apr | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | PSG5-R：caacaacgtccacacctg | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GATCTACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA CAGCGTCTCC GACCTGATGC AGCTCTCGCA GGGCGAAGAA TCTCGTGCTT TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT AAATAGCTGC GCCGATGGTT TCTACAAAGA TCGTTATGTT GATCGGCACT TTGCATCGGC CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCAAGCTTG GGCTGCAGGT CGACTCTAGA GGATCCGCGG CCGCGCGCGA TATCGAATTC GTAATCATGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTG GTTCATGTGC AGCTCCATCA GCAAAAGGGG ATGATAAGTT TATCACCACC GACTATTTGC AACAGTGCCG TTGATCGTGC TATGATCGAC TGATGTCATC AGCGGTGGAG TGCAATGTCG TGCAATACGA ATGGCGAAAA GCCGAGCTCA TCGGTCAGCT TCTCAACCTT GGGGTTACCC CCGGCGGTGT GCTGCTGGTC CACAGCTCCT TCCGTAGCGT CCGGCCCCTC GAAGATGGGC CACTTGGACT GATCGAGGCC CTGCGTGCTG CGCTGGGTCC GGGAGGGACG CTCGTCATGC CCTCGTGGTC AGGTCTGGAC GACGAGCCGT TCGATCCTGC CACGTCGCCC GTTACACCGG ACCTTGGAGT TGTCTCTGAC ACATTCTGGC GCCTGCCAAA TGTAAAGCGC AGCGCCCATC CATTTGCCTT TGCGGCAGCG GGGCCACAGG CAGAGCAGAT CATCTCTGAT CCATTGCCCC TGCCACCTCA CTCGCCTGCA AGCCCGGTCG CCCGTGTCCA TGAACTCGAT GGGCAGGTAC TTCTCCTCGG CGTGGGACAC GATGCCAACA CGACGCTGCA TCTTGCCGAG TTGATGGCAA AGGTTCCCTA TGGGGTGCCG AGACACTGCA CCATTCTTCA GGATGGCAAG TTGGTACGCG TCGATTATCT CGAGAATGAC CACTGCTGTG AGCGCTTTGC CTTGGCGGAC AGGTGGCTCA AGGAGAAGAG CCTTCAGAAG GAAGGTCCAG TCGGTCATGC CTTTGCTCGG TTGATCCGCT CCCGCGACAT TGTGGCGACA GCCCTGGGTC AACTGGGCCG AGATCCGTTG ATCTTCCTGC ATCCGCCAGA GGCGGGATGC GAAGAATGCG ATGCCGCTCG CCAGTCGATT GGCTGAGCTC ATGAGCGGAG AACGAGATGA CGTTGGAGGG GCAAGGTCGC GCTGATTGCT GGGGCAACAC GTGGAGCGGA TCGGGGATTG TCTTTCTTCA GCTCGCTGAT GATATGCTGA CGCTCAATGC CGTTTGGCCT CCGACTAACG AAAATCCCGC ATTTGGACGG CTGATCCGAT TGGCACGGCG GACGGCGAAT GGCGGAGCAG ACGCTCGTCC GGGGGCAATG AGATATGAAA AAGCCTGAAC TCACCGCGAC GTATCGATGT CGAGGTTCCT CAGGGGAGCC ACCCCAGAGA AGCCCTCGGA GCTGAGCGGA GCTATTTCCA AAGCCATGCC AGCTAGAGAC AGTGCACACC GCCAAGCATC TGCAAAACCC TCGCCTGGAG GGAAAGTGCA ATGTACGTAC GCTGGTTTCC CTCCAGAAAG GGATTCTGCG GCTTCTCAAC TTTGGAAGAA GAGGCGGAAC GAACTGCTGT TTCAGCCTAC TCATGTGAGG AGGCTGGTCC TTTACCCTGA TGCCCGAGGG CATCAGGGTA AAGGACCAGC CTCGCCAACT AGGAGCGCTA CCAAGGCGAA CACAACCCAG GACTTGGTAG AACCCTTCGC CGGAGAAGTT CAGACTCATC GGCATGAGCA CCCCTCGCCG CGCACTCGGC ACCGGTCCGT CGACCACCAC CAGGACGTCG TTGTCGACGT CGGCCCCGCG GCTCCTGCCC GCCGAACGCG TCGTCGTCGA CGGCCTGGTG CTCATCGACG AGCACCCGGA GCCAGGTGAA AAGCGCCGGC GGACGCTCGG ACTGGGCGCG GGATTCCAGC AGTAACCCAG GTCCGCCGGC CACCTCACGG CAGGCAGACC CTCGGCTTTC GCGCCGGGAC CGCCATGAGA CCGCCACCCG GATGTCCGGG GTGGCGGTCT CATGGGCGGT CCCTCAGCGG CCCTACGCGA CCGCTGTGTC GACGCGGAGG CAGTCTCCGG GGTGCTGGCC CCAGGGCTGG AGTACCGGGG CGAGCTTGCC CTTGCAGCGG CGGCACACGG GCGACGCGGC GCCGGGCGGG GGAGTCTTGA CGTCGACGGT GACCGGCTTC GGCTTCAGGC GCTTCCGAAG GCTGATCGTC GGGCGGATCG CCTCCTTCTT CGGCTGACGC ACTCGGTCGA GCGAAGCGGC CAGCTCCTCC GTGCGGGCCT CGTTGGCCTT CCGGCGCGCC TCGCGGAAAG CAGCTTCCTC CTCGGACATG ACCTCGAACC TCATCTGGTC AGCGTCAAGG TCGCCCGGCG CGGCCGGCGC TTCCGGGGCG GGCGGGTCCA GGACGTCCTT GCCCCACACC AGGCCCCAGG ACTCGACGAG CCGCCGGACG CCCGGTAGGC CGTACGTCTC GGCGACCTTG ATGAGGTCGA GGCGACGTCC GGCGACGCGG GCGATGTATC GGTACCAGAT GTAGGCCGGG ATGACCGCGA TGGCGACCAG GCCCTCGGTG TCGTCGGTGA TCTCCTCCTC GGTGCGGACG TCCTGCTGGA TGCCGAGTTC CTTGATCAGC CGGTTCAGGT TCTGCGACCG GTAGTGCTTG CGGACCTGGA AGACGCCGAA CTCGCGCTCG CGGTACTTCT CGACGAACGG GCCGGGCCGA CGAAGCCGCT GCAGCTCGGC GGCCGCCGCG TCGCCCAGGT CGAGCGGTCC CATGCGGTCG TCGCCGCGAC CGGCCTTGAA GTTCTGTCCG GCCAGCTCCA GGCCGATCTT GGCGACGCCG CCCTTGGTCT TGTCGCCGTC CTTGTAGAGG TAGCGGGCCT GCTTGCCCGC ATCGCCGTCA GCGGCGTCCG CGCCGTTGAG TGGGCGCACG TCGGTGCCGT GGCCCTTGCC CTCACAGGAG CAACCGGGCC GGTCGCACGT CTCGCTGACG GTGTAGCCGC CCGCGGATTC GACCCCGGCG GCCCAGGCTC CGGCGAGTGC GTCGCGGAAC GCGGCCTGGG CGTCCGGGCC GAGCACCTCG CGGGTGACCC AGAGCGTGTG CCAGTGCAGG TGCCAGCCGG AGCCCCAGCC GAAGGTGTCC TCGAAGGCCC GCTCGTAGCC GATGATCCCG AAGTCGTCGC GCATCGTGCG CCAGCGGCGG CCGGACGAGC CGTACGCGCC CTTCCAGCCG TCGTGCAAGA CCGCGACCAG GCCGTGCCGC ATTCCCTTGC GGACGGTGCC GAACGCCATG CGCTCGAAGT GGCGCAACGT GTTCGTGCCA AGGTGCAGCC CGTACCCGGC GTCCGCGAGA CCGTCGGCGG CGAGCTGCAC GTTCGAGCCC CGTACGGCCA GGATGCGGCT CATGCACCAC GGGCAGGTGT GGACGTTGTT GCAGCGGCAC GTGTTGCCCC ACGTCGCCTC GCCCGGCTTC CACATCAGCT CGGCCGTCCC GGCAGTGAGC CGGGTCCCGC AGCCCTTGAA CGCCTCGTTC AGCGACACCG TCTGGTGCCG GTCCCGCCGG GCGAACCGCT CGTCGCGCGG GTCCTCCCGC CCGGCTGTCG CGGCACCCTC GTTTGGGGTA GAACCCGTTC CAGTTACAGC GCTCTGACCT GCAGTGGACG GAGATTTTCC CTTACTACTA AAGCCCGCGT CCGGATTACC CGCTGTAGTC GTGCTTGCTA CGCTGCGTGA CTGGTCCGCA ATGAGACGCT TTGCGCGCTT TCGGCAGGCG TCCGAGCAGT AGATTTTGGG GCGCTTCCCG GGGATGTGGA CGATCGGGGT GCCGCAGTGG CACTTCGGTC CGGCGGGGCG CGGTGGTGTC GACGCGCTGT TCTCTCGTAC GCTCGTCACA GAGCAAACGT CCTCACTCGG CATGCTGCGC CGGTTCGGGG GCGGCGAGCC CGGGAGGCCA ATCCCGGGCT CGTGCCATTT CTGGGTCCTG TTGATCCTGG CATTGGTGTG GCCGTTCATT GCCCCTGCTC GCTCCTGACG CGCCGATAGA CGTCCGATAC GCCCGGTGCT GGTGGGATTT GATAGGTCGG AAGAAGCCCC GCCCGGGCCT GGGCGGGGCT TCCTGTGCGT CAGGACCTCC TCGTCGTGAG CCTCTTCGGC CTATGGACGG AGTGACCTCG TGATCCGTTA CAGCCGCGCG CGCTCGCGTA GAGCGGTCTC ATCAGTTCCA CGAACGGTCC TCTTCGCAGA TCAGGGCGTT GGGGCGGAGT CTCACCAAGG ACTACGTCTG CTGGCGATTT CCGTTACACC CCGGGCGGTG GCCGGCGCAC ACGCGCGCCC GCGTTGGGCA GTGCAGAAAG TGCAGAAACC TAGGCGCTGA TGGTCCAGGT CCACGGTTCG TCGTCGGCGG CGGCGCGGGC GGCGGCGTCG GCCAGGGCGC GGGCGAGACC GGCTACGGCG GGCTTGATGC GCCGGTTGCG GGCGACCTTG AGCAGCTAGT ATGCAGGTCG ACGGATCTTT TCCGCTGCAT AACCCTGCTT CGGGGTCATT ATAGCGATTT TTTCGGTATA TCCATCCTTT TTCGCACGAT ATACAGGATT TTGCCAAAGG GTTCGTGTAG ACTTTCCTTG GTGTATCCAA CGGCGTCAGC CGGGCAGGAT AGGTGAAGTA GGCCCACCCG CGAGCGGGTG TTCCTTCTTC ACTGTCCCTT ATTCGCACCT GGCGGTGCTC AACGGGAATC CTGCTCTGCG AGGCTGGCCG GCATGAGCCT GTCGGCCTAC CTGCTGGCCG TCGGCCAGGG CTACAAAATC ACGGGCGTCG TGGACTATGA GCACGTCCGC GAGCTGGCCC GCATCAATGG CGACCTGGGC CGCCTGGGCG GCCTGCTGAA ACTCTGGCTC ACCGACGACC CGCGCACGGC GCGGTTCGGT GATGCCACGA TCCTCGCCCT GCTGGCGAAG ATCGAAGAGA AGCAGGACGA GCTTGGCAAG GTCATGATGG GCGTGGTCCG CCCGAGGGCA GAGCCATGAC TTTTTTAGCC GCTAAAACGG CCGGGGGGTG CGCGTGATTG CCAAGCACGT CCCCATGCGC TCCATCAAGA AGAGCGACTT CGCGGAGCTG GTGAAGTACA TCACCGACGA GCAAGGCAAG ACCGATCCCC GGGGACCTGC ATA
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