pPink-LC
$99.00
In stock
SKU
PL82873
100μg liquid plasmid(Instantly):$120
| name | pPink-LC | another name | apPink-LC |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | ADE2 | Fluorescent labeling | |
| Promoter | AOX1 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 5'AOX：GACTGGTTCCAATTGACAAGC | |
| 3′ sequencing primer | 3'AOX：GCAAATGGCATTCTGACATCC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGGCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAACG GAATTCCGGA CCGGCATGCA GGCCTGGTAC CGGCCGGCCA TTTAAATACA GGCCCCTTTT CCTTTGTCGA TATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCTCCCACAT CCGCTCTAAC CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTTAATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAAACGC GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG GCTTTAATTT GCAAGCTGGA TCCGCGGCCG CGACTTGACT CTTCACTAGC CTATGCAAAT AAGGTTACCT TTTCCAAGAA TCGTAGAAAC GATTAAAAAA CTTCCAAACT CTCATGGATT CTCAGGTAAT AGGTATTCTA GGAGGAGGCC AGCTAGGCCG AATGATTGTT GAGGCCGCTA GCAGGCTCAA TATCAAGACC GTGATTCTTG ATGATGGTTT TTCACCTGCT AAGCACATTA ATGCTGCGCA AGACCACATC GACGGATCAT TCAAAGATGA GGAGGCTATC GCCAAGTTAG CTGCCAAATG TGATGTTCTC ACTGTAGAGA TTGAGCATGT CAACACAGAT GCTCTAAAGA GAGTTCAAGA CAGAACTGGA ATCAAGATAT ATCCTTTACC AGAGACAATC GAACTAATCA AGGATAAGTA CTTGCAAAAG GAACATTTGA TCAAGCACAA CATTTCGGTG ACAAAGTCTC AGGGTATAGA ATCTAATGAA AAGGCGCTGC TTTTGTTTGG AGAAGAGAAT GGATTTCCAT ATCTGTTGAA GTCCCGGACT ATGGCTTATG ATGGAAGAGG CAATTTTGTA GTGGAGTCTA AAGAGGACAT CAGTAAGGCA TTAGAGTTCT TGAAAGATCG TCCATTGTAT GCCGAGAAGT TTGCTCCTTT TGTTAAAGAA TTAGCGGTAA TGGTTGTGAG ATCACTGGAA GGCGAAGTAT TCTCCTACCC AACCGTAGAA ACTGTGCACA AGGACAATAT CTGTCATATT GTGTATGCTC CGGCCAGAGT TAATGACACC ATCCAAAAGA AAGCTCAAAT ATTAGCTGAA AACACTGTGA AGACTTTCCC AGGCGCTGGA ATCTTCGGAG TTGAGATGTT CCTATTGTCT GATGGAGAAC TTCTTGTAAA TGAGATTGCT CCAAGGCCCC ACAATTCTGG TCACTATACA ATCGATGCAT GTGTAACATC TCAGTTCGAA GCACATGTAA GAGCCATAAC TGGTCTGCCA ATGCCACTAG ATTTCACCAA ACTATCTACT TCCAACACCA ACGCTATTAT GCTCAATGTT TTGGGTGCTG AAAAATCTCA CGGGGAATTA GAGTTTTGTA GAAGAGCCTT AGAAACACCC GGTGCTTCTG TATATCTGTA CGGAAAGACC ACCCGATTGG CTCGTAAGAT GGGTCATATC AACATAATAG GATCTTCCAT GTTGGAAGCA GAACAAAAGT TAGAGTACAT TCTAGAAGAA TCAACCCACT TACCATCCAG TACTGTATCA GCTGACACTA AACCGTTGGT TGGAGTTATC ATGGGTTCAG ACTCTGATCT ACCTGTGATT TCGAAAGGTT GCGATATTTT AAAACAGTTT GGTGTTCCAT TCGAAGTTAC TATTGTCTCT GCTCATAGAA CACCACAGAG AATGACCAGA TATGCCTTTG AAGCCGCTAG TAGAGGTATC AAGGCTATCA TTGCAGGTGC TGGTGGTGCT GCTCATCTTC CAGGAATGGT TGCTGCCATG ACTCCGTTGC CAGTCATTGG TGTTCCTGTC AAGGGCTCTA CGTTGGATGG TGTAGACTCG CTACACTCGA TTGTCCAAAT GCCTAGAGGT GTTCCTGTGG CTACGGTTGC TATCAACAAC GCCACCAATG CCGCTCTGTT GGCCATCAGG ATTTTAGGTA CAATTGACCA CAAATGGCAA AAGGAAATGT CCAAGTATAT GAATGCAATG GAGACCGAAG TGTTGGGGAA GGCATCCAAC TTGGAATCTG AAGGGTATGA ATCCTATTTG AAGAATCGTC TTTGAATTTA GTATTGTTTT TTAATAGATG TATATATAAT AGTACACGTA ACTTATCTAT TCCATTCATA ATTTTATTTT AAAGGTTCGG TAGAAATTTG TCCTCCAAAA AGTTGGTTAG AGCCTGGCAG TTTTGATAGG CATTATTATA GATTGGGTAA TATTTACCCT GCACCTGGAG GAACTTTGCA AAGAGCCTCA TGTGCGGCGC GCCAGGCCAT AATGGCCAAA CGGTTTCTCA ATTACTATAT ACTACTAACC ATTTACCTGT AGCGTATTTC TTTTCCCTCT TCGCGAAAGC TCAAGGGCAT CTTCTTGACT CATGAAAAAT ATCTGGATTT CTTCTGACAG ATCATCACCC TTGAGCCCAA CTCTCTAGCC TATGAGTGTA AGTGATAGTC ATCTTGCAAC AGATTATTTT GGAACGCAAC TAACAAAGCA GATACACCCT TCAGCAGAAT CCTTTCTGGA TATTGTGAAG AATGATCGCC AAAGTCACAG TCCTGAGACA GTTCCTAATC TTTACCCCAT TTACAAGTTC ATCCAATCAG ACTTCTTAAC GCCTCATCTG GCTTATATCA AGCTTACCAA CAGTTCAGAA ACTCCCAGTC CAAGTTTCTT GCTTGAAAGT GCGAAGAATG GTGACACCGT TGACAGGTAC ACCTTTATGG GACATTCCCC CAGAAAAATA ATCAAGACTG GGCCTTTAGA GGGTGCTGAA GTTGACCCCT TGGTGCTTCT GGAAAAAGAA CTGAAGGGCA CCAGACAAGC GCAACTTCCT GGTATTCCTC GTCTAAGTGG TGGTGCCATA GGATACATCT CGTACGATTG TATTAAGTAC TTTGAACCAA AAACTGAAAG AAAACTGAAA GATGTTTTGC AACTTCCGGA AGCAGCTTTG ATGTTGTTCG ACACGATCGT GGCTTTTGAC AATGTTTATC AAAGATTCCA GGTAATTGGA AACGTTTCTC TATCCGTTGA TGACTCGGAC GAAGCTATTC TTGAGAAATA TTATAAGACA AGAGAAGAAG TGGAAAAGAT CAGTAAAGTG GTATTTGACA ATAAAACTGT TCCCTACTAT GAACAGAAAG ATATTATTCA AGGCCAAACG TTCACCTCTA ATATTGGTCA GGAAGGGTAT GAAAACCATG TTCGCAAGCT GAAAGAACAT ATTCTGAAAG GAGACATCTT CCAAGCTGTT CCCTCTCAAA GGGTAGCCAG GCCGACCTCA TTGCACCCTT TCAACATCTA TCGTCATTTG AGAACTGTCA ATCCTTCTCC ATACATGTTC TATATTGACT ATCTAGACTT CCAAGTTGTT GGTGCTTCAC CTGAATTACT AGTTAAATCC GACAACAACA ACAAAATCAT CACACATCCT ATTGCTGGAA CTCTTCCCAG AGGTAAAACT ATCGAAGAGG ACGACAATTA TGCTAAGCAA TTGAAGTCGT CTTTGAAAGA CAGGGCCGAG CACGTCATGC TGGTAGATTT GGCCAGAAAT GATATTAACC GTGTGTGTGA GCCCACCAGT ACCACGGTTG ATCGTTTATT GACTGTGGAG AGATTTTCTC ATGTGATGCA TCTTGTGTCA GAAGTCAGTG GAACATTGAG ACCAAACAAG ACTCGCTTCG ATGCTTTCAG ATCCATTTTC CCAGCAGGAA CCGTCTCCGG TGCTCCGAAG GTAAGAGCAA TGCAACTCAT AGGAGAATTG GAAGGAGAAA AGAGAGGTGT TTATGCGGGG GCCGTAGGAC ACTGGTCGTA CGATGGAAAA TCGATGGACA CATGTATTGC CTTAAGAACA ATGGTCGTCA AGGACGGTGT CGCTTACCTT CAAGCCGGAG GTGGAATTGT CTACGATTCT GACCCCTATG ACGAGTACAT CGAAACCATG AACAAAATGA GATCCAACAA TAACACCATC TTGGAGGCTG AGAAAATCTG GACCGATAGG TTGGCCAGAG ACGAGAATCA AAGTGAATCC GAAGAAAACG ATCAATGAAC GGAGGACGTA AGTAGGAATT TATGGTTTGG CCATAATGGC CTAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TG
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