pRK415
$128.00
In stock
SKU
PL82814
100μg liquid plasmid(Instantly):$163
| name | pRK415 | another name | apRK415 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Tet | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | oriV | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13R：CAGGAAACAGCTATGACC | |
| 3′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTGCCATTTT TGGGGTGAGG CCGTTCGCGG CCGAGGGGCG CAGCCCCTGG GGGGATGGGA GGCCCGCGTT AGCGGGCCGG GAGGGTTCGA GAAGGGGGGG CACCCCCCTT CGGCGTGCGC GGTCACGCGC ACAGGGCGCA GCCCTGGTTA AAAACAAGGT TTATAAATAT TGGTTTAAAA GCAGGTTAAA AGACAGGTTA GCGGTGGCCG AAAAACGGGC GGAAACCCTT GCAAATGCTG GATTTTCTGC CTGTGGACAG CCCCTCAAAT GTCAATAGGT GCGCCCCTCA TCTGTCAGCA CTCTGCCCCT CAAGTGTCAA GGATCGCGCC CCTCATCTGT CAGTAGTCGC GCCCCTCAAG TGTCAATACC GCAGGGCACT TATCCCCAGG CTTGTCCACA TCATCTGTGG GAAACTCGCG TAAAATCAGG CGTTTTCGCC GATTTGCGAG GCTGGCCAGC TCCACGTCGC CGGCCGAAAT CGAGCCTGCC CCTCATCTGT CAACGCCGCG CCGGGTGAGT CGGCCCCTCA AGTGTCAACG TCCGCCCCTC ATCTGTCAGT GAGGGCCAAG TTTTCCGCGA GGTATCCACA ACGCCGGCGG CCGCGGTGTC TCGCACACGG CTTCGACGGC GTTTCTGGCG CGTTTGCAGG GCCATAGACG GCCGCCAGCC CAGCGGCGAG GGCAACCAGC CCGGTGAGCG TCGGAAAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCGAGCTC GGTACCCGGG GATCCTCTAG AGTCGACCTG CAGGCATGCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCG GTCTTGCCTT GCTCGTCGGT GATGTACTTC ACCAGCTCCG CGAAGTCGCT CTTCTTGATG GAGCGCATGG GGACGTGCTT GGCAATCACG CGCACCCCCC GGCCGTTTTA GCGGCTAAAA AAGTCATGGC TCTGCCCTCG GGCGGACCAC GCCCATCATG ACCTTGCCAA GCTCGTCCTG CTTCTCTTCG ATCTTCGCCA GCAGGGCGAG GATCGTGGCA TCACCGAACC GCGCCGTGCG CGGGTCGTCG GTGAGCCAGA GTTTCAGCAG GCCGCCCAGG CGGCCCAGGT CGCCATTGAT GCGGGCCAGC TCGCGGACGT GCTCATAGTC CACGACGCCC GTGATTTTGT AGCCCTGGCC GACGGCCAGC AGGTAGGCCG ACAGGCTCAT GCCGGCCGCC GCCGCCTTTT CCTCAATCGC TCTTCGTTCG TCTGGAAGGC AGTACACCTT GATAGGTGGG CTGCCCTTCC TGGTTGGCTT GGTTTCATCA GCCATCCGCT TGCCCTCATC TGTTACGCCG GCGGTAGCCG GCCAGCCTCG CAGAGCAGGA TTCCCGTTGA GCACCGCCAG GTGCGAATAA GGGACAGTGA AGAAGGAACA CCCGCTCGCG GGTGGGCCTA CTTCACCTAT CCTGCCCGGC TGACGCCGTT GGATACACCA AGGAAAGTCT ACACGAACCC TTTGGCAAAA TCCTGTATAT CGTGCGAAAA AGGATGGATA TACCGAAAAA ATCGCTATAA TGACCCCGAA GCAGGGTTAT GCAGCGGAAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCAGAA GGCCGCCAGA GAGGCCGAGC GCGGCCGTGA GGCTTGGACG CTAGGGCAGG GCATGAAAAA GCCCGTAGCG GGCTGCTACG GGCGTCTGAC GCGGTGGAAA GGGGGAGGGG ATGTTGTCTA CATGGCTCTG CTGTAGTGAG TGGGTTGCGC TCCGGCAGCG GTCCTGATCA ATCGTCACCC TTTCTCGGTC CTTCAACGTT CCTGACAACG AGCCTCCTTT TCGCCAATCC ATCGACAATC ACCGCGAGTC CCTGCTCGAA CGCTGCGTCC GGACCGGCTT CGTCGAAGGC GTCTATCGCG GCCCGCAACA GCGGCGAGAG CGGAGCCTGT TCAACGGTGC CGCCGCGCTC GCCGGCATCG CTGTCGCCGG CCTGCTCCTC AAGCACGGCC CCAACAGTGA AGTAGCTGAT TGTCATCAGC GCATTGACGG CGTCCCCGGC CGAAAAACCC GCCTCGCAGA GGAAGCGAAG CTGCGCGTCG GCCGTTTCCA TCTGCGGTGC GCCCGGTCGC GTGCCGGCAT GGATGCGCGC GCCATCGCGG TAGGCGAGCA GCGCCTGCCT GAAGCTGCGG GCATTCCCGA TCAGAAATGA GCGCCAGTCG TCGTCGGCTC TCGGCACCGA ATGCGTATGA TTCTCCGCCA GCATGGCTTC GGCCAGTGCG TCGAGCAGCG CCCGCTTGTT CCTGAAGTGC CAGTAAAGCG CCGGCTGCTG AACCCCCAAC CGTTCCGCCA GTTTGCGTGT CGTCAGACCG TCTACGCCGA CCTCGTTCAA CAGGTCCAGG GCGGCACGGA TCACTGTATT CGGCTGCAAC TTTGTCATGC TTGACACTTT ATCACTGATA AACATAATAT GTCCACCAAC TTATCAGTGA TAAAGAATCC GCGCGTTCAA TCGGACCAGC GGAGGCTGGT CCGGAGGCCA GACATGAAAC CCAACATACC CCTGATCGTA ATTCTGAGCA CTGTCGCGCT CGACGCTGTC GGCATCGGCC TGATTATGCC GGTGCTGCCG GGCCTCCTGC GCGATCTGGT TCACTCGAAC GACGTCACCG CCCACTATGG CATTCTGCTG GCGCTGTATG CGTTGGTGCA ATTTGCCTGC GCACCTGTGC TGGGCGCGCT GTCGGATCGT TTCGGGCGGC GGCCAATCTT GCTCGTCTCG CTGGCCGGCG CCACTGTCGA CTACGCCATC ATGGCGACAG CGCCTTTCCT TTGGGTTCTC TATATCGGGC GGATCGTGGC CGGCATCACC GGGGCGACTG GGGCGGTAGC CGGCGCTTAT ATTGCCGATA TCACTGATGG CGATGAGCGC GCGCGGCACT TCGGCTTCAT GAGCGCCTGT TTCGGGTTCG GGATGGTCGC GGGACCTGTG CTCGGTGGGC TGATGGGCGG TTTCTCCCCC CACGCTCCGT TCTTCGCCGC GGCAGCCTTG AACGGCCTCA ATTTCCTGAC GGGCTGTTTC CTTTTGCCGG AGTCGCACAA AGGCGAACGC CGGCCGTTAC GCCGGGAGGC TCTCAACCCG CTCGCTTCGT TCCGGTGGGC CCGGGGCATG ACCGTCGTCG CCGCCCTGAT GGCGGTCTTC TTCATCATGC AACTTGTCGG ACAGGTGCCG GCCGCGCTTT GGGTCATTTT CGGCGAGGAT CGCTTTCACT GGGACGCGAC CACGATCGGC ATTTCGCTTG CCGCATTTGG CATTCTGCAT TCACTCGCCC AGGCAATGAT CACCGGCCCT GTAGCCGCCC GGCTCGGCGA AAGGCGGGCA CTCATGCTCG GAATGATTGC CGACGGCACA GGCTACATCC TGCTTGCCTT CGCGACACGG GGATGGATGG CGTTCCCGAT CATGGTCCTG CTTGCTTCGG GTGGCATCGG AATGCCGGCG CTGCAAGCAA TGTTGTCCAG GCAGGTGGAT GAGGAACGTC AGGGGCAGCT GCAAGGCTCA CTGGCGGCGC TCACCAGCCT GACCTCGATC GTCGGACCCC TCCTCTTCAC GGCGATCTAT GCGGCTTCTA TAACAACGTG GAACGGGTGG GCATGGATTG CAGGCGCTGC CCTCTACTTG CTCTGCCTGC CGGCGCTGCG TCGCGGGCTT TGGAGCGGCG CAGGGCAACG AGCCGATCGC TGATCGTGGA AACGATAGGC CTATGCCATG CGGGTCAAGG CGACTTCCGG CAAGCTATAC GCGCCCTAGG AGTGCGGTTG GAACGTTGGC CCAGCCAGAT ACTCCCGATC ACGAGCAGGA CGCCGATGAT TTGAAGCGCA CTCAGCGTCT GATCCAAGAA CAACCATCCT AGCAACACGG CGGTCCCCGG GCTGAGAAAG CCCAGTAAGG AAACAACTGT AGGTTCGAGT CGCGAGATCC CCCGGAACCA AAGGAAGTAG GTTAAACCCG CTCCGATCAG GCCGAGCCAC GCCAGGCCGA GAACATTGGT TCCTGTAGGC ATCGGGATTG GCGGATCAAA CACTAAAGCT ACTGGAACGA GCAGAAGTCC TCCGGCCGCC AGTTGCCAGG CGGTAAAGGT GAGCAGAGGC ACGGGAGGTT GCCACTTGCG GGTCAGCACG GTTCCGAACG CCATGGAAAC CGCCCCCGCC AGGCCCGCTG CGACGCCGAC AGGATCTAGC GCTGCGTTTG GTGTCAACAC CAACAGCGCC ACGCCCGCAG TTCCGCAAAT AGCCCCCAGG ACCGCCATCA ATCGTATCGG GCTACCTAGC AGAGCGGCAG AGATGAACAC GACCATCAGC GGCTGCACAG CGCCTACCGT CGCCGCGACC CGCCCGGCAG GCGGTAGACC GAAATAAACA ACAAGCTCCA GAATAGCGAA ATATTAAGTG CGCCGAGGAT GAAGATGCGC ATCCACCAGA TTCCCGTTGG AATCTGTCGG ACGATCATCA CGAGCAATAA ACCCGCCGGC AACGCCCGCA GCAGCATACC GGCGACCCCT CGGCCTCGCT GTTCGGGCTC CACGAAAACG CCGGACAGAT GCGCCTTGTG AGCGTCCTTG GGGCCGTCCT CCTGTTTGAA GACCGACAGC CCAATGATCT CGCCGTCGAT GTAGGCGCCG AATGCCACGG CATCTCGCAA CCGTTCAGCG AACGCCTCCA TGGGCTTTTT CTCCTCGTGC TCGTAAACGG ACCCGAACAT CTCTGGAGCT TTCTTCAGGG CCGACAATCG GATCTCGCGG AAATCCTGCA CGTCGGCCGC TCCAAGCCGT CGAATCTGAG CCTTAATCAC AATTGTCAAT TTTAATCCTC TGTTTATCGG CAGTTCGTAG AGCGCGCCGT GCGCCCGAGC GATACTGAGC GAAGCAAGTG CGTCGAGCAG TGCCCGCTTG TTCCTGAAAT GCCAGTAAAG CGCTGGCTGC TGAACCCCCA GCCGGAACTG ACCCCACAAG GCCCTAGCGT TTGCAATGCA CCAGGTCATC ATTGACCCAG GCGTGTTCCA CCAGGCCGCT GCCTCGCAAC TCTTCGCAGG CTTCGCCGAC CTGCTCGCGC CACTTCTTCA CGCGGGTGGA ATCCGATCCG CACATGAGGC GGAAGGTTTC CAGCTTGAGC GGGTACGGCT CCCGGTGCGA GCTGAAATAG TCGAACATCC GTCGGGCCGT CGGCGACAGC TTGCGGTACT TCTCCCATAT GAATTTCGTG TAGTGGTCGC CAGCAAACAG CACGACGATT TCCTCGTCGA TCAGGACCTG GCAACGGGAC GTTTTCTTGC CACGGTCCAG GACGCGGAAG CGGTGCAGCA GCGACACCGA TTCCAGGTGC CCAACGCGGT CGGACGTGAA GCCCATCGCC GTCGCCTGTA GGCGCGACAG GCATTCCTCG GCCTTCGTGT AATACCGGCC ATTGATCGAC CAGCCCAGGT CCTGGCAAAG CTCGTAGAAC GTGAAGGTGA TCGGCTCGCC GATAGGGGTG CGCTTCGCGT ACTCCAACAC CTGCTGCCAC ACCAGTTCGT CATCGTCGGC CCGCAGCTCG ACGCCGGTGT AGGTGATCTT CACGTCCTTG TTGACGTGGA AAATGACCTT GTTTTGCAGC GCCTCGCGCG GGATTTTCTT GTTGCGCGTG GTGAACAGGG CAGAGCGGGC CGTGTCGTTT GGCATCGCTC GCATCGTGTC CGGCCACGGC GCAATATCGA ACAAGGAAAG CTGCATTTCC TTGATCTGCT GCTTCGTGTG TTTCAGCAAC GCGGCCTGCT TGGCCTCGCT GACCTGTTTT GCCAGGTCCT CGCCGGCGGT TTTTCGCTTC TTGGTCGTCA TAGTTCCTCG CGTGTCGATG GTCATCGACT TCGCCAAACC TGCCGCCTCC TGTTCGAGAC GACGCGAACG CTCCACGGCG GCCGATGGCG CGGGCAGGGC AGGGGGAGCC AGTTGCACGC TGTCGCGCTC GATCTTGGCC GTAGCTTGCT GGACCATCGA GCCGACGGAC TGGAAGGTTT CGCGGGGCGC ACGCATGACG GTGCGGCTTG CGATGGTTTC GGCATCCTCG GCGGAAAACC CCGCGTCGAT CAGTTCTTGC CTGTATGCCT TCCGGTCAAA CGTCCGATTC ATTCACCCTC CTTGCGGGAT TGCCCCGACT CACGCCGGGG CAATGTGCCC TTATTCCTGA TTTGACCCGC CTGGTGCCTT GGTGTCCAGA TAATCCACCT TATCGGCAAT GAAGTCGGTC CCGTAGACCG TCTGGCCGTC CTTCTCGTAC TTGGTATTCC GAATCTTGCC CTGCACGAAT ACCAGCGACC CCTTGCCCAA ATACTTGCCG TGGGCCTCGG CCTGAGAGCC AAAACACTTG ATGCGGAAGA AGTCGGTGCG CTCCTGCTTG TCGCCGGCAT CGTTGCGCCA CTCTTCATTA ACCGCTATAT CGAAAATTGC TTGCGGCTTG TTAGAATTGC CATGACGTAC CTCGGTGTCA CGGGTAAGAT TACCGATAAA CTGGAACTGA TTATGGCTCA TATCGAAAGT CTCCTTGAGA AAGGAGACTC TAGTTTAGCT AAACATTGGT TCCGCTGTCA AGAACTTTAG CGGCTAAAAT TTTGCGGGCC GCGACCAAAG GTGCGAGGGG CGGCTTCCGC TGTGTACAAC CAGATATTTT TCACCAACAT CCTTCGTCTG CTCGATGAGC GGGGCATGAC GAAACATGAG CTGTCGGAGA GGGCAGGGGT TTCAATTTCG TTTTTATCAG ACTTAACCAA CGGTAAGGCC AACCCCTCGT TGAAGGTGAT GGAGGCCATT GCCGACGCCC TGGAAACTCC CCTACCTCTT CTCCTGGAGT CCACCGACCT TGACCGCGAG GCACTCGCGG AGATTGCGGG TCATCCTTTC AAGAGCAGCG TGCCGCCCGG ATACGAACGC ATCAGTGTGG TTTTGCCGTC ACATAAGGCG TTTATCGTAA AGAAATGGGG CGACGACACC CGAAAAAAGC TGCGTGGAAG GCTCTGACGC CAAGGGTTAG GGCTTGCACT TCCTTCTTTA GCCGCTAAAA CGGCCCCTTC TCTGCGGGCC GTCGGCTCGC GCATCATATC GACATCCTCA ACGGAAGCCG TGCCGCGAAT GGCATCGGGC GGGTGCGCTT TGACAGTTGT TTTCTATCAG AACCCCTACG TCGTGCGGTT CGATTAGCTG TTTGTCTTGC AGGCTAAACA CTTTCGGTAT ATCGTTTGCC TGTGCGATAA TGTTGCTAAT GATTTGTTGC GTAGGGGTTA CTGAAAAGTG AGCGGGAAAG AAGAGTTTCA GACCATCAAG GAGCGGGCCA AGCGCAAGCT GGAACGCGAC ATGGGTGCGG ACCTGTTGGC CGCGCTCAAC GACCCGAAAA CCGTTGAAGT CATGCTCAAC GCGGACGGCA AGGTGTGGCA CGAACGCCTT GGCGAGCCGA TGCGGTACAT CTGCGACATG CGGCCCAGCC AGTCGCAGGC GATTATAGAA ACGGTGGCCG GATTCCACGG CAAAGAGGTC ACGCGGCATT CGCCCATCCT GGAAGGCGAG TTCCCCTTGG ATGGCAGCCG CTTTGCCGGC CAATTGCCGC CGGTCGTGGC CGCGCCAACC TTTGCGATCC GCAAGCGCGC GGTCGCCATC TTCACGCTGG AACAGTACGT CGAGGCGGGC ATCATGACCC GCGAGCAATA CGAGGTCATT AAAAGCGCCG TGATTGATGA TATAGCGGCC CGGCTGCTCC TGGTTCTCGC GCACCGAAAT GGGTGACTTC ACCCCGCGCT CTTTGATCGT GGCACCGATT TCCGCGATGC TCTCCGGGGA AAAGCCGGGG TTGTCGGCCG TCCGCGGCTG ATGCGGATCT TCGTCGATCA GGTCCAGGTC CAGCTCGATA GGGCCGGAAC CGCCCTGAGA CGCCGCAGGA GCGTCCAGGA GGCTCGACAG GTCGCCGATG CTATCCAACC CCAGGCCGGA CGGCTGCGCC GCGCCTGCGG CTTCCTGAGC GGCCGCAGCG GTGTTTTTCT TGGTGGTCTT GGCTTGAGCC GCAGTCATTG GGAAATCTCC ATCTTCGTGA ACACGTAATC AGCCAGGGCG CGAACCTCTT TCGATGCCTT GCGCGCGGCC GTTTTCTTGA TCTTCCAGAC CGGCACACCG GATGCGAGGG CATCGGCGAT GCTGCTGCGC AGGCCAACGG TGGCCGGAAT CATCATCTTG GGGTACGCGG CCAGCAGCTC GGCTTGGTGG CGCGCGTGGC GCGGATTCCG CGCATCGACC TTGCTGGGCA CCATGCCAAG GAATTGCAGC TTGGCGTTCT TCTGGCGCAC GTTCGCAATG GTCGTGACCA TCTTCTTGAT GCCCTGGATG CTGTACGCCT CAAGCTCGAT GGGGGACAGC ACATAGTCGG CCGCGAAGAG GGCGGCCGCC AGGCCGACGC CAAGGGTCGG GGCCGTGTCG ATCAGGCACA CGTCGAAGCC TTGGTTCGCC AGGGCCTTGA TGTTCGCCCC GAACAGCTCG CGGGCGTCGT CCAGCGACAG CCGTTCGGCG TTCGCCAGTA CCGGGTTGGA CTCGATGAGG GCGAGGCGCG CGGCCTGGCC GTCGCCGGCT GCGGGTGCGG TTTCGGTCCA GCCGCCGGCA GGGACAGCGC CGAACAGCTT GCTTGCATGC AGGCCGGTAG CAAAGTCCTT GAGCGTGTAG GACGCATTGC CCTGGGGGTC CAGGTCGATC ACGGCAACCC GCAAGCCGCG CTCGAAAAAG TCGAAGGCAA GATGCACAAG GGTCGAAGTC TTGCCGACGC CGCCTTTCTG GTTGGCCGTG ACCAAAGTTT TCATCGTTTG GTTTCCTGTT TTTTCTTGGC GTCCGCTTCC CACTTCCGGA CGATGTACGC CTGATGTTCC GGCAGAACCG CCGTTACCCG CGCGTACCCC TCGGGCAAGT TCTTGTCCTC GAACGCGGCC CACACGCGAT GCACCGCTTG CGACACTGCG CCCCTGGTCA GTCCCAGCGA CGTTGCGAAC GTCGCCTGTG GCTTCCCATC GACTAAGACG CCCCGCGCTA TCTCGATGGT CTGCTGCCCC ACTTCCAGCC CCTGGATCGC CTCCTGGAAC TGGCTTTCGG TAAGCCGTTT CTTCATGGAT AACACCCATA ATTTGCTCCG CGCCTTGGTT GAACATAGCG GTGACAGCCG CCAGCACATG AGAGAAGTTT AGCTAAACAT TTCTCGCACG TCAACACCTT TAGCCGCTAA AACTCGTCCT TGGCGTAACA AAACAAAAGC CCGGAAACCG GGCTTTCGTC TCTTGCCGCT TATGGCTCTG CACCCGGCTC CATCACCAAC AGGTCGCGCA CGCGCTTCAC TCGGTTGCGG ATCGACACTG CCAGCCCAAC AAAGCCGGTT GCCGCCGCCG CCAGGATCGC GCCGATGATG CCGGCCACAC CGGCCATCGC CCACCAGGTC GCCGCCCTCA CTGCCCGGCA CCTGGTCGCT GAATGTCGAT GCCAGCACCT GCGGCACGTC AATGCTTCCG GGCGTCGCGC TCGGGCTGAT CGCCCATCCC GTTACTGCCC CGATCCCGGC AATGGCAAGG ACTGCCAGCG
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