pCAMBIA1300
$128.00
In stock
SKU
PL82812
100μg liquid plasmid(Instantly):$163
| name | pCAMBIA1300 | another name | apCAMBIA1300 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | Replicon | pVS1 | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTCGAGCT CGGTACCCGG GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTGGCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGCTAGA GCAGCTTGAG CTTGGATCAG ATTGTCGTTT CCCGCCTTCA GTTTAAACTA TCAGTGTTTG ACAGGATATA TTGGCGGGTA AACCTAAGAG AAAAGAGCGT TTATTAGAAT AACGGATATT TAAAAGGGCG TGAAAAGGTT TATCCGTTCG TCCATTTGTA TGTGCATGCC AACCACAGGG TTCCCCTCGG GATCAAAGTA CTTTGATCCA ACCCCTCCGC TGCTATAGTG CAGTCGGCTT CTGACGTTCA GTGCAGCCGT CTTCTGAAAA CGACATGTCG CACAAGTCCT AAGTTACGCG ACAGGCTGCC GCCCTGCCCT TTTCCTGGCG TTTTCTTGTC GCGTGTTTTA GTCGCATAAA GTAGAATACT TGCGACTAGA ACCGGAGACA TTACGCCATG AACAAGAGCG CCGCCGCTGG CCTGCTGGGC TATGCCCGCG TCAGCACCGA CGACCAGGAC TTGACCAACC AACGGGCCGA ACTGCACGCG GCCGGCTGCA CCAAGCTGTT TTCCGAGAAG ATCACCGGCA CCAGGCGCGA CCGCCCGGAG CTGGCCAGGA TGCTTGACCA CCTACGCCCT GGCGACGTTG TGACAGTGAC CAGGCTAGAC CGCCTGGCCC GCAGCACCCG CGACCTACTG GACATTGCCG AGCGCATCCA GGAGGCCGGC GCGGGCCTGC GTAGCCTGGC AGAGCCGTGG GCCGACACCA CCACGCCGGC CGGCCGCATG GTGTTGACCG TGTTCGCCGG CATTGCCGAG TTCGAGCGTT CCCTAATCAT CGACCGCACC CGGAGCGGGC GCGAGGCCGC CAAGGCCCGA GGCGTGAAGT TTGGCCCCCG CCCTACCCTC ACCCCGGCAC AGATCGCGCA CGCCCGCGAG CTGATCGACC AGGAAGGCCG CACCGTGAAA GAGGCGGCTG CACTGCTTGG CGTGCATCGC TCGACCCTGT ACCGCGCACT TGAGCGCAGC GAGGAAGTGA CGCCCACCGA GGCCAGGCGG CGCGGTGCCT TCCGTGAGGA CGCATTGACC GAGGCCGACG CCCTGGCGGC CGCCGAGAAT GAACGCCAAG AGGAACAAGC ATGAAACCGC ACCAGGACGG CCAGGACGAA CCGTTTTTCA TTACCGAAGA GATCGAGGCG GAGATGATCG CGGCCGGGTA CGTGTTCGAG CCGCCCGCGC ACGTCTCAAC CGTGCGGCTG CATGAAATCC TGGCCGGTTT GTCTGATGCC AAGCTGGCGG CCTGGCCGGC CAGCTTGGCC GCTGAAGAAA CCGAGCGCCG CCGTCTAAAA AGGTGATGTG TATTTGAGTA AAACAGCTTG CGTCATGCGG TCGCTGCGTA TATGATGCGA TGAGTAAATA AACAAATACG CAAGGGGAAC GCATGAAGGT TATCGCTGTA CTTAACCAGA AAGGCGGGTC AGGCAAGACG ACCATCGCAA CCCATCTAGC CCGCGCCCTG CAACTCGCCG GGGCCGATGT TCTGTTAGTC GATTCCGATC CCCAGGGCAG TGCCCGCGAT TGGGCGGCCG TGCGGGAAGA TCAACCGCTA ACCGTTGTCG GCATCGACCG CCCGACGATT GACCGCGACG TGAAGGCCAT CGGCCGGCGC GACTTCGTAG TGATCGACGG AGCGCCCCAG GCGGCGGACT TGGCTGTGTC CGCGATCAAG GCAGCCGACT TCGTGCTGAT TCCGGTGCAG CCAAGCCCTT ACGACATATG GGCCACCGCC GACCTGGTGG AGCTGGTTAA GCAGCGCATT GAGGTCACGG ATGGAAGGCT ACAAGCGGCC TTTGTCGTGT CGCGGGCGAT CAAAGGCACG CGCATCGGCG GTGAGGTTGC CGAGGCGCTG GCCGGGTACG AGCTGCCCAT TCTTGAGTCC CGTATCACGC AGCGCGTGAG CTACCCAGGC ACTGCCGCCG CCGGCACAAC CGTTCTTGAA TCAGAACCCG AGGGCGACGC TGCCCGCGAG GTCCAGGCGC TGGCCGCTGA AATTAAATCA AAACTCATTT GAGTTAATGA GGTAAAGAGA AAATGAGCAA AAGCACAAAC ACGCTAAGTG CCGGCCGTCC GAGCGCACGC AGCAGCAAGG CTGCAACGTT GGCCAGCCTG GCAGACACGC CAGCCATGAA GCGGGTCAAC TTTCAGTTGC CGGCGGAGGA TCACACCAAG CTGAAGATGT ACGCGGTACG CCAAGGCAAG ACCATTACCG AGCTGCTATC TGAATACATC GCGCAGCTAC CAGAGTAAAT GAGCAAATGA ATAAATGAGT AGATGAATTT TAGCGGCTAA AGGAGGCGGC ATGGAAAATC AAGAACAACC AGGCACCGAC GCCGTGGAAT GCCCCATGTG TGGAGGAACG GGCGGTTGGC CAGGCGTAAG CGGCTGGGTT GTCTGCCGGC CCTGCAATGG CACTGGAACC CCCAAGCCCG AGGAATCGGC GTGAGCGGTC GCAAACCATC CGGCCCGGTA CAAATCGGCG CGGCGCTGGG TGATGACCTG GTGGAGAAGT TGAAGGCCGC GCAGGCCGCC CAGCGGCAAC GCATCGAGGC AGAAGCACGC CCCGGTGAAT CGTGGCAAGC GGCCGCTGAT CGAATCCGCA AAGAATCCCG GCAACCGCCG GCAGCCGGTG CGCCGTCGAT TAGGAAGCCG CCCAAGGGCG ACGAGCAACC AGATTTTTTC GTTCCGATGC TCTATGACGT GGGCACCCGC GATAGTCGCA GCATCATGGA CGTGGCCGTT TTCCGTCTGT CGAAGCGTGA CCGACGAGCT GGCGAGGTGA TCCGCTACGA GCTTCCAGAC GGGCACGTAG AGGTTTCCGC AGGGCCGGCC GGCATGGCCA GTGTGTGGGA TTACGACCTG GTACTGATGG CGGTTTCCCA TCTAACCGAA TCCATGAACC GATACCGGGA AGGGAAGGGA GACAAGCCCG GCCGCGTGTT CCGTCCACAC GTTGCGGACG TACTCAAGTT CTGCCGGCGA GCCGATGGCG GAAAGCAGAA AGACGACCTG GTAGAAACCT GCATTCGGTT AAACACCACG CACGTTGCCA TGCAGCGTAC GAAGAAGGCC AAGAACGGCC GCCTGGTGAC GGTATCCGAG GGTGAAGCCT TGATTAGCCG CTACAAGATC GTAAAGAGCG AAACCGGGCG GCCGGAGTAC ATCGAGATCG AGCTAGCTGA TTGGATGTAC CGCGAGATCA CAGAAGGCAA GAACCCGGAC GTGCTGACGG TTCACCCCGA TTACTTTTTG ATCGATCCCG GCATCGGCCG TTTTCTCTAC CGCCTGGCAC GCCGCGCCGC AGGCAAGGCA GAAGCCAGAT GGTTGTTCAA GACGATCTAC GAACGCAGTG GCAGCGCCGG AGAGTTCAAG AAGTTCTGTT TCACCGTGCG CAAGCTGATC GGGTCAAATG ACCTGCCGGA GTACGATTTG AAGGAGGAGG CGGGGCAGGC TGGCCCGATC CTAGTCATGC GCTACCGCAA CCTGATCGAG GGCGAAGCAT CCGCCGGTTC CTAATGTACG GAGCAGATGC TAGGGCAAAT TGCCCTAGCA GGGGAAAAAG GTCGAAAAGG TCTCTTTCCT GTGGATAGCA CGTACATTGG GAACCCAAAG CCGTACATTG GGAACCGGAA CCCGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG TCACACATGT AAGTGACTGA TATAAAAGAG AAAAAAGGCG ATTTTTCCGC CTAAAACTCT TTAAAACTTA TTAAAACTCT TAAAACCCGC CTGGCCTGTG CATAACTGTC TGGCCAGCGC ACAGCCGAAG AGCTGCAAAA AGCGCCTACC CTTCGGTCGC TGCGCTCCCT ACGCCCCGCC GCTTCGCGTC GGCCTATCGC GGCCGCTGGC CGCTCAAAAA TGGCTGGCCT ACGGCCAGGC AATCTACCAG GGCGCGGACA AGCCGCGCCG TCGCCACTCG ACCGCCGGCG CCCACATCAA GGCACCCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGCAT TCTAGGTACT AAAACAATTC ATCCAGTAAA ATATAATATT TTATTTTCTC CCAATCAGGC TTGATCCCCA GTAAGTCAAA AAATAGCTCG ACATACTGTT CTTCCCCGAT ATCCTCCCTG ATCGACCGGA CGCAGAAGGC AATGTCATAC CACTTGTCCG CCCTGCCGCT TCTCCCAAGA TCAATAAAGC CACTTACTTT GCCATCTTTC ACAAAGATGT TGCTGTCTCC CAGGTCGCCG TGGGAAAAGA CAAGTTCCTC TTCGGGCTTT TCCGTCTTTA AAAAATCATA CAGCTCGCGC GGATCTTTAA ATGGAGTGTC TTCTTCCCAG TTTTCGCAAT CCACATCGGC CAGATCGTTA TTCAGTAAGT AATCCAATTC GGCTAAGCGG CTGTCTAAGC TATTCGTATA GGGACAATCC GATATGTCGA TGGAGTGAAA GAGCCTGATG CACTCCGCAT ACAGCTCGAT AATCTTTTCA GGGCTTTGTT CATCTTCATA CTCTTCCGAG CAAAGGACGC CATCGGCCTC ACTCATGAGC AGATTGCTCC AGCCATCATG CCGTTCAAAG TGCAGGACCT TTGGAACAGG CAGCTTTCCT TCCAGCCATA GCATCATGTC CTTTTCCCGT TCCACATCAT AGGTGGTCCC TTTATACCGG CTGTCCGTCA TTTTTAAATA TAGGTTTTCA TTTTCTCCCA CCAGCTTATA TACCTTAGCA GGAGACATTC CTTCCGTATC TTTTACGCAG CGGTATTTTT CGATCAGTTT TTTCAATTCC GGTGATATTC TCATTTTAGC CATTTATTAT TTCCTTCCTC TTTTCTACAG TATTTAAAGA TACCCCAAGA AGCTAATTAT AACAAGACGA ACTCCAATTC ACTGTTCCTT GCATTCTAAA ACCTTAAATA CCAGAAAACA GCTTTTTCAA AGTTGTTTTC AAAGTTGGCG TATAACATAG TATCGACGGA GCCGATTTTG AAACCGCGGT GATCACAGGC AGCAACGCTC TGTCATCGTT ACAATCAACA TGCTACCCTC CGCGAGATCA TCCGTGTTTC AAACCCGGCA GCTTAGTTGC CGTTCTTCCG AATAGCATCG GTAACATGAG CAAAGTCTGC CGCCTTACAA CGGCTCTCCC GCTGACGCCG TCCCGGACTG ATGGGCTGCC TGTATCGAGT GGTGATTTTG TGCCGAGCTG CCGGTCGGGG AGCTGTTGGC TGGCTGGTGG CAGGATATAT TGTGGTGTAA ACAAATTGAC GCTTAGACAA CTTAATAACA CATTGCGGAC GTTTTTAATG TACTGAATTA ACGCCGAATT AATTCGGGGG ATCTGGATTT TAGTACTGGA TTTTGGTTTT AGGAATTAGA AATTTTATTG ATAGAAGTAT TTTACAAATA CAAATACATA CTAAGGGTTT CTTATATGCT CAACACATGA GCGAAACCCT ATAGGAACCC TAATTCCCTT ATCTGGGAAC TACTCACACA TTATTATGGA GAAACTCGAG CTTGTCGATC GACAGATCCG GTCGGCATCT ACTCTATTTC TTTGCCCTCG GACGAGTGCT GGGGCGTCGG TTTCCACTAT CGGCGAGTAC TTCTACACAG CCATCGGTCC AGACGGCCGC GCTTCTGCGG GCGATTTGTG TACGCCCGAC AGTCCCGGCT CCGGATCGGA CGATTGCGTC GCATCGACCC TGCGCCCAAG CTGCATCATC GAAATTGCCG TCAACCAAGC TCTGATAGAG TTGGTCAAGA CCAATGCGGA GCATATACGC CCGGAGTCGT GGCGATCCTG CAAGCTCCGG ATGCCTCCGC TCGAAGTAGC GCGTCTGCTG CTCCATACAA GCCAACCACG GCCTCCAGAA GAAGATGTTG GCGACCTCGT ATTGGGAATC CCCGAACATC GCCTCGCTCC AGTCAATGAC CGCTGTTATG CGGCCATTGT CCGTCAGGAC ATTGTTGGAG CCGAAATCCG CGTGCACGAG GTGCCGGACT TCGGGGCAGT CCTCGGCCCA AAGCATCAGC TCATCGAGAG CCTGCGCGAC GGACGCACTG ACGGTGTCGT CCATCACAGT TTGCCAGTGA TACACATGGG GATCAGCAAT CGCGCATATG AAATCACGCC ATGTAGTGTA TTGACCGATT CCTTGCGGTC CGAATGGGCC GAACCCGCTC GTCTGGCTAA GATCGGCCGC AGCGATCGCA TCCATAGCCT CCGCGACCGG TTGTAGAACA GCGGGCAGTT CGGTTTCAGG CAGGTCTTGC AACGTGACAC CCTGTGCACG GCGGGAGATG CAATAGGTCA GGCTCTCGCT AAACTCCCCA ATGTCAAGCA CTTCCGGAAT CGGGAGCGCG GCCGATGCAA AGTGCCGATA AACATAACGA TCTTTGTAGA AACCATCGGC GCAGCTATTT ACCCGCAGGA CATATCCACG CCCTCCTACA TCGAAGCTGA AAGCACGAGA TTCTTCGCCC TCCGAGAGCT GCATCAGGTC GGAGACGCTG TCGAACTTTT CGATCAGAAA CTTCTCGACA GACGTCGCGG TGAGTTCAGG CTTTTTCATA TCTCATTGCC CCCCGGGATC TGCGAAAGCT CGAGAGAGAT AGATTTGTAG AGAGAGACTG GTGATTTCAG CGTGTCCTCT CCAAATGAAA TGAACTTCCT TATATAGAGG AAGGTCTTGC GAAGGATAGT GGGATTGTGC GTCATCCCTT ACGTCAGTGG AGATATCACA TCAATCCACT TGCTTTGAAG ACGTGGTTGG AACGTCTTCT TTTTCCACGA TGCTCCTCGT GGGTGGGGGT CCATCTTTGG GACCACTGTC GGCAGAGGCA TCTTGAACGA TAGCCTTTCC TTTATCGCAA TGATGGCATT TGTAGGTGCC ACCTTCCTTT TCTACTGTCC TTTTGATGAA GTGACAGATA GCTGGGCAAT GGAATCCGAG GAGGTTTCCC GATATTACCC TTTGTTGAAA AGTCTCAATA GCCCTTTGGT CTTCTGAGAC TGTATCTTTG ATATTCTTGG AGTAGACGAG AGTGTCGTGC TCCACCATGT TCACATCAAT CCACTTGCTT TGAAGACGTG GTTGGAACGT CTTCTTTTTC CACGATGCTC CTCGTGGGTG GGGGTCCATC TTTGGGACCA CTGTCGGCAG AGGCATCTTG AACGATAGCC TTTCCTTTAT CGCAATGATG GCATTTGTAG GTGCCACCTT CCTTTTCTAC TGTCCTTTTG ATGAAGTGAC AGATAGCTGG GCAATGGAAT CCGAGGAGGT TTCCCGATAT TACCCTTTGT TGAAAAGTCT CAATAGCCCT TTGGTCTTCT GAGACTGTAT CTTTGATATT CTTGGAGTAG ACGAGAGTGT CGTGCTCCAC CATGTTGGCA AGCTGCTCTA GCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACG
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
