pHG52d34.5-CMV-mIL-15-mCherry
$113.00
In stock
SKU
PL82794
NM_008357.2
100μg liquid plasmid(Instantly):$134
| name | pHG52d34.5-CMV-mIL-15-mCherry | another name | apHG52d34.5-CMV-mIL-15-mCherry |
| Insert Size (bp) | 486bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Red | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATCCGTGTAA CCGCCAGTCC GTGCTTGCCT AGCGAACTCA CCCGTCCCGG CTGGCGTGCG CAGCCCGGGC CGTGTTGCGG GCCCTCTTAA GGGGCGGCGG CAGGACGGGG ACTCCCGCCC CGCCTCTTTT CCCCCGGGGA GTCAACCCCC GGGGGGGGTG TTTTTTGGGG GGGGGCGCGA AGGCGGGCGG CGGCGGCGGG CGGGCGGCAG GGCAGCCCCG CGCGCCCCCT TCCCCGTCCC TCCCCCGGAG CCGGCCGCTC CCCCGCGGGC GCCGCCCCTC CCCCCGCGCG CCGCGGGGCT GCCTTCCCGC GGGCGCCCCC GCGCGGCTTT TTTCCCGCGC CCGCCCCCGC GCGGCAGGAC GGGGACTAGC AGGCTGTGCC GCAGACCACC ACACACTCCC AAGCTCCCCG CCCCCCCGAA GACGCCAGTC GCACCACCGC TCGCCCTCGC AGACCAGACA GTTGCACCAA GCACCCGCCC GCCCGCACAC GGTTCCCCGC CACCCCCTCC CTCCCCTCCA TCCCGCCGAG CTCGCGGCAG CCCCTCCCCC CCGCGCGCCA CGGGGCTGCG GTCCCGCGGC CGCCTCCCCC GCGGCCGCCT CCCCCGCGCC CCGCCCCGGG GGCTTCCCCC GCCCCTCCCC CCGCGCCCGC GGCCCCGAGC TCGCAGCAGC CCCTCCCTCC CGCGCCCCGT GCCTTCCCTC CCGCTCCTGC GGGGGGGCTC GGGCCACCTG ACCTTCGTAA CCTGCACTCA GGTCAGAGAG ATCTGCCGGT CTCCCTATAG TGAGTCGTAT TAATTTCGAT AAGCCAGGTT AACCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGGAC ATATTGTCGT TAGAACGCGG CTACAATTAA TACATAACCT TATGTATCAT ACACATACGA TTTAGGTGAC ACTATAGAAC TCGAGCAGCT GCGGTGAAGG TCGTCGTCAG AGATTCCCAC CTCGGTCTCC TCCTCCGAGT CGCTGCTGGC GAGCCACTGC ATGTCGTTGA GCATCCCCCA GGCGTGCGGG GCGGCGGGCT GCTTGACAAA GCAACGGGGG GGATTTAGAG GGCGCGGGGC GTGAGGCGGG ACCCCCGCGC CGTGTCCCCC GTGTCCCTCC CTCACCCCGG CCCCCCGCCC GCTGCTTTTT GTTCGGAAGG GGGGGAGAAA GGGGTCCGTA ACCAAAGGTG GTCTGCGTCC TTTGGATTCC GACCCCTCGT CTCCCCCCCT GTCCCCCGCT CTCGGGCTCC TCCCTGCCTC CCTCGCCCCC CCAGAGGGTC GGGGGGCGGC GCACGGCCCA CGGGGGTCCC CCGACCGCTT AAGCGGGCCG GGGGTCGGCC CCGTCAAGCG TCCCCGCCCC CGAGCCCACC GCCCGCGACC ACCCCCAACC CGCAGCCGGG TGGTCCGGGG AAAAGGGGGG GCCTGAGACC CGGGGGTCGC CCTCTCACCG TGCCGGGGGT CTGCCGCGGC GGCCGCTCGG GGCCGGGGTC CGCCCGGGAG CTCGTGCCGG GCCGGGGTTC CATGAGCCGG GGTAGGGTAG ACTCGAGACG GCGGCCCGCG GTCTCTCTCT TGCCGGGTTT TAGTCTCTGT CTCTCCGGGT CTCCTCCTCC CGCCGGGCCG CCGCTCCGTC GCTCGCAGTG CCGGGGTGCG AATGCGGCCC GACCGTCACA CGGGGCTGCC TTATACCCGG CGCCTATCCA CTCCCCCAAA GGGGCGGCAT TTACGATTCC CCCAATAGCC GCGCGCCCCG GCGGGGGCGG AGGGAGGGAA TCCCCCCCTC TCGGGGCGGC CCCGTCCCCG GGGACCAACC GGGTGTACTC CAAGAACCCC ATTAGCATGC GCCGCCCCCC GCCGACGCAG ATGGGAGTCC CCCCGGCGCC CCGCCGGCGC GGCCCTGAGT GGTGCCCGCC CCCGGGGAAA AATTCATTAG CATACTAGGA AGCCCAGGGG ACCAATAGGG GCCGATCAGC CCACCCACCC GGCGGCGCGC GAGGCTCTGC GTGTTCTGCC AAGAAAGTAA TCAGCATAAC CCGGAACCCC GAGGGAGTAA TTACGCGGGG AGCGAGGGGC CGTCCGAACG TTTTTAATTA CCATAAGCGG GAATGGCGGC CCGTTAAAAG CTGCTAATTA CCGCGAGCGG GAACGCCGGC CCATTAAAAG TTGCTAATTA CCATGCGCGG GGATGGCGGC CGGGACCGCC TATTAAAAGT TTCTAATTAC CATACCGGGA AGCCGGCTCT AGAGGATCCC CGGGTACCGA GCTCGAATTC ATCGATGATC GATGTACGGG CCAGATATAC GCGTTGACAT TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA CTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG ACTCACTATA GGGAGACCCA AGCTTGGTAC CGAGCTCGGA TCCACTAGTA ACGGCCGCCA GTGTGCTGGA ATTCTGCAGA TATCCATCAC ACTGGCGGCC AGCTTCGAAT TCTGCAGTCG ACGGTACCGC GGGCCCGGGA TCCACCGGTC GCCACCATGA AAATTTTGAA ACCATATATG AGGAATACAT CCATCTCGTG CTACTTGTGT TTCCTTCTAA ACAGTCACTT TTTAACTGAG GCTGGCATTC ATGTCTTCAT TTTGGGCTGT GTCAGTGTAG GTCTCCCTAA AACAGAGGCC AACTGGATAG ATGTAAGATA TGACCTGGAG AAAATTGAAA GCCTTATTCA ATCTATTCAT ATTGACACCA CTTTATACAC TGACAGTGAC TTTCATCCCA GTTGCAAAGT TACTGCAATG AACTGCTTTC TCCTGGAATT GCAGGTTATT TTACATGAGT ACAGTAACAT GACTCTTAAT GAAACAGTAA GAAACGTGCT CTACCTTGCA AACAGCACTC TGTCTTCTAA CAAGAATGTA GCAGAATCTG GCTGCAAGGA ATGTGAGGAG CTGGAGGAGA AAACCTTCAC AGAGTTTTTG CAAAGCTTTA TACGCATTGT CCAAATGTTC ATCAACACGT CCGCCACGAA CTTCTCTCTG TTAAAGCAAG CAGGAGACGT GGAAGAAAAC CCCGGTCCTA TGGTGAGCAA GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGTAA AGCGGCCCTG GCGGCCGCTC GAGCATGCAT CTAGAGGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAG
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