pRS423
$128.00
In stock
SKU
PL82792
100μg liquid plasmid(Instantly):$149
| name | pRS423 | another name | apRS423 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | HIS3 | Fluorescent labeling | |
| Promoter | Replicon | 2U | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGATGAT CCAATATCAA AGGAAATGAT AGCATTGAAG GATGAGACTA ATCCAATTGA GGAGTGGCAG CATATAGAAC AGCTAAAGGG TAGTGCTGAA GGAAGCATAC GATACCCCGC ATGGAATGGG ATAATATCAC AGGAGGTACT AGACTACCTT TCATCCTACA TAAATAGACG CATATAAGTA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT TCCGAAGTTC CTATTCTCTA GAAAGTATAG GAACTTCAGA GCGCTTTTGA AAACCAAAAG CGCTCTGAAG ACGCACTTTC AAAAAACCAA AAACGCACCG GACTGTAACG AGCTACTAAA ATATTGCGAA TACCGCTTCC ACAAACATTG CTCAAAAGTA TCTCTTTGCT ATATATCTCT GTGCTATATC CCTATATAAC CTACCCATCC ACCTTTCGCT CCTTGAACTT GCATCTAAAC TCGACCTCTA CATTTTTTAT GTTTATCTCT AGTATTACTC TTTAGACAAA AAAATTGTAG TAAGAACTAT TCATAGAGTG AATCGAAAAC AATACGAAAA TGTAAACATT TCCTATACGT AGTATATAGA GACAAAATAG AAGAAACCGT TCATAATTTT CTGACCAATG AAGAATCATC AACGCTATCA CTTTCTGTTC ACAAAGTATG CGCAATCCAC ATCGGTATAG AATATAATCG GGGATGCCTT TATCTTGAAA AAATGCACCC GCAGCTTCGC TAGTAATCAG TAAACGCGGG AAGTGGAGTC AGGCTTTTTT TATGGAAGAG AAAATAGACA CCAAAGTAGC CTTCTTCTAA CCTTAACGGA CCTACAGTGC AAAAAGTTAT CAAGAGACTG CATTATAGAG CGCACAAAGG AGAAAAAAAG TAATCTAAGA TGCTTTGTTA GAAAAATAGC GCTCTCGGGA TGCATTTTTG TAGAACAAAA AAGAAGTATA GATTCTTTGT TGGTAAAATA GCGCTCTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT GTTTGAAAAA TTAGCGCTCT CGCGTTGCAT TTTTGTTTTA CAAAAATGAA GCACAGATTC TTCGTTGGTA AAATAGCGCT TTCGCGTTGC ATTTCTGTTC TGTAAAAATG CAGCTCAGAT TCTTTGTTTG AAAAATTAGC GCTCTCGCGT TGCATTTTTG TTCTACAAAA TGAAGCACAG ATGCTTCGTT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA CCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCCTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCG CGCAATTAAC CCTCACTAAA GGGAACAAAA GCTGGAGCTC CACCGCGGTG GCGGCCGCTC TAGAACTAGT GGATCCCCCG GGCTGCAGGA ATTCGATATC AAGCTTATCG ATACCGTCGA CCTCGAGGGG GGGCCCGGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC GCGCGCTCAC TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT AACAAAATAT TAACGTTTAC AATTTCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATAGAT CCGTCGAGTT CAAGAGAAAA AAAAAGAAAA AGCAAAAAGA AAAAAGGAAA GCGCGCCTCG TTCAGAATGA CACGTATAGA ATGATGCATT ACCTTGTCAT CTTCAGTATC ATACTGTTCG TATACATACT TACTGACATT CATAGGTATA CATATATACA CATGTATATA TATCGTATGC TGCAGCTTTA AATAATCGGT GTCACTACAT AAGAACACCT TTGGTGGAGG GAACATCGTT GGTACCATTG GGCGAGGTGG CTTCTCTTAT GGCAACCGCA AGAGCCTTGA ACGCACTCTC ACTACGGTGA TGATCATTCT TGCCTCGCAG ACAATCAACG TGGAGGGTAA TTCTGCTAGC CTCTGCAAAG CTTTCAAGAA AATGCGGGAT CATCTCGCAA GAGAGATCTC CTACTTTCTC CCTTTGCAAA CCAAGTTCGA CAACTGCGTA CGGCCTGTTC GAAAGATCTA CCACCGCTCT GGAAAGTGCC TCATCCAAAG GCGCAAATCC TGATCCAAAC CTTTTTACTC CACGCGCCAG TAGGGCCTCT TTAAAAGCTT GACCGAGAGC AATCCCGCAG TCTTCAGTGG TGTGATGGTC GTCTATGTGT AAGTCACCAA TGCACTCAAC GATTAGCGAC CAGCCGGAAT GCTTGGCCAG AGCATGTATC ATATGGTCCA GAAACCCTAT ACCTGTGTGG ACGTTAATCA CTTGCGATTG TGTGGCCTGT TCTGCTACTG CTTCTGCCTC TTTTTCTGGG AAGATCGAGT GCTCTATCGC TAGGGGACCA CCCTTTAAAG AGATCGCAAT CTGAATCTTG GTTTCATTTG TAATACGCTT TACTAGGGCT TTCTGCTCTG TCATCTTTGC CTTCGTTTAT CTTGCCTGCT CATTTTTTAG TATATTCTTC GAAGAAATCA CATTACTTTA TATAATGTAT AATTCATTAT GTGATAATGC CAATCGCTAA GAAAAAAAAA GAGTCATCCG CTAGGGGAAA AAAAAAAATG AAAATCATTA CCGAGGCATA AAAAAATATA GAGTGTACTA GAGGAGGCCA AGAGTAATAG AAAAAGAAAA TTGCGGGAAA GGACTGTGTT ATGACTTCCC TGACTAATGC CGTGTTCAAA CGATACCTGG CAGTGACTCC TAGCGCTCAC CAAGCTCTTA AAACGGGAAT TTATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGA
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