pME6032
$128.00
In stock
SKU
PL82789
100μg liquid plasmid(Instantly):$163
| name | pME6032 | another name | apME6032 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Tet |
| Selective marker | Fluorescent labeling | ||
| Promoter | Tac | Replicon | p15A |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | lacIq-F：gcccggaagagagtcaat | |
| 3′ sequencing primer | Tac-P（2022.2.18Synthesis）：gacaattaatcatcggctcg | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGATCCAACC CCTCCGCTGC TATAGTGCAG TCGGCTTCTG ACGTTCAGTG CAGCCGTCTT CTGAAAACGA CATGTCGCAC AAGTCCTAAG TTACGCGACA GGCTGCCGCC CTGCCCTTTT CCTGGCGTTT TCTTGTCGCG TGTTTTAGTC GCATAAAGTA GAATACTTGC GACTAGAACC GGAGACATTA CGCCATGAAC AAGAGCGCCG CCGCTGGCCT GCTGGGCTAT GCCCGCGTCA GCACCGACGA CCAGGACTTG ACCAACCAAC GGGCCGAACT GCACGCGGCC GGCTGCACCA AGCTGTTTTC CGAGAAGATC ACCGGCACCA GGCGCGACCG CCCGGAGCTG GCCAGGATGC TTGACCACCT ACGCCCTGGC GACGTTGTGA CAGTGACCAG GCTAGACCGC CTGGCCCGCA GCACCCGCGA CCTACTGGAC ATTGCCGAGC GCATCCAGGA GGCCGGCGCG GGCCTGCGTA GCCTGGCAGA GCCGTGGGCC GACACCACCA CGCCGGCCGG CCGCATGGTG TTGACCGTGT TCGCCGGCAT TGCCGAGTTC GAGCGTTCCC TAATCATCGA CCGCACCCGG AGCGGGCGCG AGGCCGCCAA GGCCCGAGGC GTGAAGTTTG GCCCCCGCCC TACCCTCACC CCGGCACAGA TCGCGCACGC CCGCGAGCTG ATCGACCAGG AAGGCCGCAC CGTGAAAGAG GCGGCTGCAC TGCTTGGCGT GCATCGCTCG ACCCTGTACC GCGCACTTGA GCGCAGCGAG GAAGTGACGC CCACCGAGGC CAGGCGGCGC GGTGCCTTCC GTGAGGACGC ATTGACCGAG GCCGACGCCC TGGCGGCCGC CGAGAATGAA CGCCAAGAGG AACAAGCATG AAACCGCACC AGGACGGCCA GGACGAACCG TTTTTCATTA CCGAAGAGAT CGAGGCGGAG ATGATCGCGG CCGGGTACGT GTTCGAGCCG CCCGCGCACG TCTCAACCGT GCGGCTGCAT GAAATCCTGG CCGGTTTGTC TGATGCCAAG CTGGCGGCCT GGCCGGCCAG CTTGGCCGCT GAAGAAACCG AGCGCCGCCG TCTAAAAAGG TGATGTGTAT TTGAGTAAAA CAGCTTGCGT CATGCGGTCG CTGCGTATAT GATGCGATGA GTAAATAAAC AAATACGCAA GGGGAACGCA TGAAGGTTAT CGCTGTACTT AACCAGAAAG GCGGGTCAGG CAAGACGACC ATCGCAACCC ATCTAGCCCG CGCCCTGCAA CTCGCCGGGG CCGATGTTCT GTTAGTCGAT TCCGATCCCC AGGGCAGTGC CCGCGATTGG GCGGCCGTGC GGGAAGATCA ACCGCTAACC GTTGTCGGCA TCGACCGCCC GACGATTGAC CGCGACGTGA AGGCCATCGG CCGGCGCGAC TTCGTAGTGA TCGACGGAGC GCCCCAGGCG GCGGACTTGG CTGTGTCCGC GATCAAGGCA GCCGACTTCG TGCTGATTCC GGTGCAGCCA AGCCCTTACG ACATATGGGC CACCGCCGAC CTGGTGGAGC TGGTTAAGCA GCGCATTGAG GTCACGGATG GAAGGCTACA AGCGGCCTTT GTCGTGTCGC GGGCGATCAA AGGCACGCGC ATCGGCGGTG AGGTTGCCGA GGCGCTGGCC GGGTACGAGC TGCCCATTCT TGAGTCCCGT ATCACGCAGC GCGTGAGCTA CCCAGGCACT GCCGCCGCCG GCACAACCGT TCTTGAATCA GAACCCGAGG GCGACGCTGC CCGCGAGGTC CAGGCGCTGG CCGCTGAAAT TAAATCAAAA CTCATTTGAG TTAATGAGGT AAAGAGAAAA TGAGCAAAAG CACAAACACG CTAAGTGCCG GCCGTCCGAG CGCACGCAGC AGCAAGGCTG CAACGTTGGC CAGCCTGGCA GACACGCCAG CCATGAAGCG GGTCAACTTT CAGTTGCCGG CGGAGGATCA CACCAAGCTG AAGATGTACG CGGTACGCCA AGGCAAGACC ATTACCGAGC TGCTATCTGA ATACATCGCG CAGCTACCAG AGTAAATGAG CAAATGAATA AATGAGTAGA TGAATTTTAG CGGCTAAAGG AGGCGGCATG GAAAATCAAG AACAACCAGG CACCGACGCC GTGGAATGCC CCATGTGTGG AGGAACGGGC GGTTGGCCAG GCGTAAGCGG CTGGGTTGTC TGCCGGCCCT GCAATGGCAC TGGAACCCCC AAGCCCGAGG AATCGGCGTG AGCGGTCGCA AACCATCCGG CCCGGTACAA ATCGGCGCGG CGCTGGGTGA TGACCTGGTG GAGAAGTTGA AGGCCGCGCA GGCCGCCCAG CGGCAACGCA TCGAGGCAGA AGCACGCCCC GGTGAATCGT GGCAAGCGGC CGCTGATCGA ATCCGCAAAG AATCCCGGCA ACCGCCGGCA GCCGGTGCGC CGTCGATTAG GAAGCCGCCC AAGGGCGACG AGCAACCAGA TTTTTTCGTT CCGATGCTCT ATGACGTGGG CACCCGCGAT AGTCGCAGCA TCATGGACGT GGCCGTTTTC CGTCTGTCGA AGCGTGACCG ACGAGCTGGC GAGGTGATCC GCTACGAGCT TCCAGACGGG CACGTAGAGG TTTCCGCAGG GCCGGCCGGC ATGGCCAGTG TGTGGGATTA CGACCTGGTA CTGATGGCGG TTTCCCATCT AACCGAATCC ATGAACCGAT ACCGGGAAGG GAAGGGAGAC AAGCCCGGCC GCGTGTTCCG TCCACACGTT GCGGACGTAC TCAAGTTCTG CCGGCGAGCC GATGGCGGAA AGCAGAAAGA CGACCTGGTA GAAACCTGCA TTCGGTTAAA CACCACGCAC GTTGCCATGC AGCGTACGAA GAAGGCCAAG AACGGCCGCC TGGTGACGGT ATCCGAGGGT GAAGCCTTGA TTAGCCGCTA CAAGATCGTA AAGAGCGAAA CCGGGCGGCC GGAGTACATC GAGATCGAGC TAGCTGATTG GATGTACCGC GAGATCACAG AAGGCAAGAA CCCGGACGTG CTGACGGTTC ACCCCGATTA CTTTTTGATC GATCCCGGCA TCGGCCGTTT TCTCTACCGC CTGGCACGCC GCGCCGCAGG CAAGGCAGAA GCCAGATGGT TGTTCAAGAC GATCTACGAA CGCAGTGGCA GCGCCGGAGA GTTCAAGAAG TTCTGTTTCA CCGTGCGCAA GCTGATCGGG TCAAATGACC TGCCGGAGTA CGATTTGAAG GAGGAGGCGG GGCAGGCTGG CCCGATCCTA GTCATGCGCT ACCGCAACCT GATCGAGGGC GAAGCATCCG CCGGTTCCTA ATGTACGGAG CAGATGCTAG GGCAAATTGC CCTAGCAGGG GAAAAAGGTC GAAAAGGTCT CTTTCCTGTG GATAGCACGT ACATTGGGAA CCCAAAGCCG TACATTGGGA ACCGGAACCC GTACATTGGG AACCCAAAGC CGTACATTGG GAACCGGTCA CACATGTAAG TGACTGATAT AAAAGAGAAA AAAGGCGATT TTTCCGCCTA AAACTCTTTA AAACTTATTA AAACTCTTAA AACCCGCCTG GCCTGTGCAT AACTGTCTGG CCAGCGCACA GCCGAAGAGC TGCAAAAAGC GCCTACCCTT CGGTCGCTGC GCTCCCTACG CCCCGCCGCT TCGCGTCGGC CTATCGCGGC CGCTGGCCGC TCAAAAATGG CTGGCCTACG GCCAGGCAAT CTACCAGGGC GCGGACAAGC CGCGCCGTCG CCACTCGACC GCCGGCGCTG AGGTCTGCCT CGTGAAGAAG GTGTTGCTGA CTCATACCAG GGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCTTAATAA GATGATCTTC TTGAGATCGT TTTGGTCTGC GCGTAATCTC TTGCTCTGAA AACGAAAAAA CCGCCTTGCA GGGCGGTTTT TCGAAGGTTC TCTGAGCTAC CAACTCTTTG AACCGAGGTA ACTGGCTTGG AGGAGCGCAG TCACCAAAAC TTGTCCTTTC AGTTTAGCCT TAACCGGCGC ATGACTTCAA GACTAACTCC TCTAAATCAA TTACCAGTGG CTGCTGCCAG TGGTGCTTTT GCATGTCTTT CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGAC TGAACGGGGG GTTCGTGCAT ACAGTCCAGC TTGGAGCGAA CTGCCTACCC GGAACTGAGT GTCAGGCGTG GAATGAGACA AACGCGGCCA TAACAGCGGA ATGACACCGG TAAACCGAAA GGCAGGAACA GGAGAGCGCA CGAGGGAGCC GCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC ACTGATTTGA GCGTCAGATT TCGTGATGCT TGTCAGGGGG GCGGAGCCTA TGGAAAAACG GCTTTGCCGC GGCCCTCTCA CTTCCCTGTT AAGTATCTTC CTGGCATCTT CCAGGAAATC TCCGCCCCGT TCGTAAGCCA TTTCCGCTCG CCGCAGTCGA ACGACCGAGC GTAGCGAGTC AGTGAGCGAG GAAGCGGAAT ATATCCTGTA TCACATATTC TGCTGACGCA CCGGTGCAGC CTTTTTTCTC CTGCCACATG AAGCACTTCA CTGACACCCT CATCAGTGCC AACATAGTAA GCCAGTATAC ACTCCGCTAG CGCTGAGGTC TGCCTCGTGA AGAAGGTGTT GCTGACTCAT ACCAGGCTAG TCCGAGGAGC TTTATGCTTG TAAACCGTTT TGTGAAAAAA TTTTTAAAAT AAAAAAGGGG ACCTCTAGGG TCCCCAATTA ATTAGTAATA TAATCTATTA AAGGTCATTC AAAAGGTCAT CCACCGGATC AATTCCCCTG CTCGCGCAGG CTGGGTGCCA AGCTCTCGGG TAACATCAAG GCCCGATCCT TGGAGCCCTT GCCCTCCCGC ACGATGATCG TGCCGTGATC GAAATCCAGA TCCTTGACCC GCAGTTGCAA ACCCTCACTG ATCCGCTAGT CCGAGGCCTC GAGATCTATC GATGCATGCC ATGGTACCCG GGAGCTCGAA TTCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACATTATA CGAGCCGATG ATTAATTGTC AACAGCTCAT TTCAGAATAT TTGCCAGAAC CGTTATGATG TCGGCGCAAA AAACATTATC CAGAACGGGA GTGCGCCTTG AGCGACACGA ATTATGCAGT GATTTACGAC CTGCACAGCC ATACCACAGC TTCCGATGGC TGCCTGACGC CAGAAGCATT GGTGCACCGT GCAGTCGATA AGCCCGGATC AGCTTGCAAT TCGCGCGCGA AGGCGAAGCG GCATGCATTT ACGTTGACAC CATCGAATGG TGCAAAACCT TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT CAGGGTGGTG AATGTGAAAC CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC TTATCAGACC GTTTCCCGCG TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA AAAAGTGGAA GCGGCGATGG CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT GGCGGGCAAA CAGTCGTTGC TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC GTCGCAAATT GTCGCGGCGA TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT GTCGATGGTA GAACGAAGCG GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC GCAACGCGTC AGTGGGCTGA TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT GGAAGCTGCC TGCACTAATG TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT CAACAGTATT ATTTTCTCCC ATGAAGACGG TACGCGACTG GGCGTGGAGC ATCTGGTCGC ATTGGGTCAC CAGCAAATCG CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA AATATCTCAC TCGCAATCAA ATTCAGCCGA TAGCGGAACG GGAAGGCGAC TGGAGTGCCA TGTCCGGTTT TCAACAAACC ATGCAAATGC TGAATGAGGG CATCGTTCCC ACTGCGATGC TGGTTGCCAA CGATCAGATG GCGCTGGGCG CAATGCGCGC CATTACCGAG TCCGGGCTGC GCGTTGGTGC GGATATCTCG GTAGTGGGAT ACGACGATAC CGAAGACAGC TCATGTTATA TCCCGCCGTT AACCACCATC AAACAGGATT TTCGCCTGCT GGGGCAAACC ACGTGGACCG CTTGCTGCAA CTCTCTCAGG GCCAGGCGGT GAAGGGCAAT CAGCTGTTGC CCGTCTCACT GGTGAAAAGA AAAACCACCC TGGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTAAGTTAG CGCGAATTGC AAGCTGGCCG ACGCGCTGGG CTACGTCTTG CTGGCGTTCG GATCCGATAT CGCCGTGGCG GCTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGGCTT TGTTGAATAA ATCGAACTTT TGCTGAGTTG AAGGATCAGA TCACGCATCT TCCCGACAAC GCAGACCGTT CCGTGGCAAA GCAAAAGTTC AAAATCACCA ACTGGTCCAC CTACAACAAA GCTCTCATCA ACCGTGGCTC CCTCACTTTC TGGCTGGATG ATGGGGCGAT TCAGGCCTGG TATGAGTCAG CAACACCTTC TTCACGAGGC AGACCTCAGC GCCAGGCGCA CGAGGCCGGC GCGCGGACCC GCGCCACGGC CCACGAGCGG ACGCCGCAGC AGGAGCGCCA GAAGGCCGCC AGAGAGGCCG AGCGCGGCCG TGAGGCTTGG ACGCTAGGGC AGGGCATGAA AAAGCCCGTA GCGGGCTGCT ACGGGCGTCT GACGCGGTGG AAAGGGGGAG GGGATGTTGT CTACATGGCT CTGCTGTAGT GAGTGGGTTG CGCTCCGGCA GCGGTCCTGA TCAATCGTCA CCCTTTCTCG GTCCTTCAAC GTTCCTGACA ACGAGCCTCC TTTTCGCCAA TCCATCGACA ATCACCGCGA GTCCCTGCTC GAACGCTGCG TCCGGACCGG CTTCGTCGAA GGCGTCTATC GCGGCCCGCA ACAGCGGCGA GAGCGGAGCC TGTTCAACGG TGCCGCCGCG CTCGCCGGCA TCGCTGTCGC CGGCCTGCTC CTCAAGCACG GCCCCAACAG TGAAGTAGCT GATTGTCATC AGCGCATTGA CGGCGTCCCC GGCCGAAAAA CCCGCCTCGC AGAGGAAGCG AAGCTGCGCG TCGGCCGTTT CCATCTGCGG TGCGCCCGGT CGCGTGCCGG CATGGATGCG CGCGCCATCG CGGTAGGCGA GCAGCGCCTG CCTGAAGCTG CGGGCATTCC CGATCAGAAA TGAGCGCCAG TCGTCGTCGG CTCTCGGCAC CGAATGCGTA TGATTCTCCG CCAGCATGGC TTCGGCCAGT GCGTCGAGCA GCGCCCGCTT GTTCCTGAAG TGCCAGTAAA GCGCCGGCTG CTGAACCCCC AACCGTTCCG CCAGTTTGCG TGTCGTCAGA CCGTCTACGC CGACCTCGTT CAACAGGTCC AGGGCGGCAC GGATCACTGT ATTCGGCTGC AACTTTGTCA TGCTTGACAC TTTATCACTG ATAAACATAA TATGTCCACC AACTTATCAG TGATAAAGAA TCCGCGCGTT CAATCGGACC AGCGGAGGCT GGTCCGGAGG CCAGACGTGA AACCCAACAT ACCCCTGATC GTAATTCTGA GCACTGTCGC GCTCGACGCT GTCGGCATCG GCCTGATTAT GCCGGTGCTG CCGGGCCTCC TGCGCGATCT GGTTCACTCG AACGACGTCA CCGCCCACTA TGGCATTCTG CTGGCGCTGT ATGCGTTGGT GCAATTTGCC TGCGCACCTG TGCTGGGCGC GCTGTCGGAT CGTTTCGGGC GGCGGCCAAT CTTGCTCGTC TCGCTGGCCG GCGCCACTGT CGACTACGCC ATCATGGCGA CAGCGCCTTT CCTTTGGGTT CTCTATATCG GGCGGATCGT GGCCGGCATC ACCGGGGCGA CTGGGGCGGT AGCCGGCGCT TATATTGCCG ATATCACTGA TGGCGATGAG CGCGCGCGGC ACTTCGGCTT CATGAGCGCC TGTTTCGGGT TCGGGATGGT CGCGGGACCT GTGCTCGGTG GGCTGATGGG CGGTTTCTCC CCCCACGCTC CGTTCTTCGC CGCGGCAGCC TTGAACGGCC TCAATTTCCT GACGGGCTGT TTCCTTTTGC CGGAGTCGCA CAAAGGCGAA CGCCGGCCGT TACGCCGGGA GGCTCTCAAC CCGCTCGCTT CGTTCCGGTG GGCCCGGGGC ATGACCGTCG TCGCCGCCCT GATGGCGGTC TTCTTCATCA TGCAACTTGT CGGACAGGTG CCGGCCGCGC TTTGGGTCAT TTTCGGCGAG GATCGCTTTC ACTGGGACGC GACCACGATC GGCATTTCGC TTGCCGCATT TGGCATTCTG CATTCACTCG CCCAGGCAAT GATCACCGGC CCTGTAGCCG CCCGGCTCGG CGAAAGGCGG GCACTCATGC TCGGAATGAT TGCCGACGGC ACAGGCTACA TCCTGCTTGC CTTCGCGACA CGGGGATGGA TGGCGTTCCC GATCATGGTC CTGCTTGCTT CGGGTGGCAT CGGAATGCCG GCGCTGCAAG CAATGTTGTC CAGGCAGGTG GATGAGGAAC GTCAGGGGCA GCTGCAAGGC TCACTGGCGG CGCTCACCAG CCTGACCTCG ATCGTCGGAC CCCTCCTCTT CACGGCGATC TATGCGGCTT CTATAACAAC GTGGAACGGG TGGGCATGGA TTGCAGGCGC TGCCCTCTAC TTGCTCTGCC TGCCGGCGCT GCGTCGCGGG CTTTGGAGCG GCGCAGGGCA ACGAGCCGAT CGCTGATCGT GGAAACGATA GGCCGCTCTA GAACTAGCTA GAACTA
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