pMIR-Report Luciferase
$99.00
In stock
SKU
PL82776
100μg liquid plasmid(Instantly):$120
| name | pMIR-Report Luciferase | another name | apMIR-Report Luciferase |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Signal report | Fragment type | miRNA,UTR |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Fluc |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pMIR-Report | 5′ sequencing primer | CMV-F： |
| 3′ sequencing primer | M13F： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTAGCGGCC GCATACAAAA AACCAACACA CAGATCCAAT GAAAATAAAA GATCCTTTAT TAAGCTTAAC TGTTTAAACC TATGATATCA CGCACGCGTC TATGCCGGCC TATGAGCTCC CTCACTAGTG CGCAGCTTTC ATCACTCGAG CAATTTGGAC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT TTCTTGCGTC GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC CGCGCAACTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT CGGGAAGACC TGCCACGCCC GCGTCGAAGA TGTTGGGGTG TTGTAACAAT ATCGATTCCA ATTCAGCGGG GGCCACCTGA TATCCTTTGT ATTTAATTAA AGACTTCAAG CGGTCAACTA TGAAGAAGTG TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT TGTCAATCAA GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGTC CTCTGACACA TAATTCGCCT CTCTGATTAA CGCCCAGCGT TTTCCCGGTA TCCAGATCCA CAACCTTCGC TTCAAAAAAT GGAACAACTT TACCGACCGC GCCCGGTTTA TCATCCCCCT CGGGTGTAAT CAGAATAGCT GATGTAGTCT CAGTGAGCCC ATATCCTTGT CGTATCCCTG GAAGATGGAA GCGTTTTGCA ACCGCTTCCC CGACTTCTTT CGAAAGAGGT GCGCCCCCAG AAGCAATTTC GTGTAAATTA GATAAATCGT ATTTGTCAAT CAGAGTGCTT TTGGCGAAGA ATGAAAATAG GGTTGGTACT AGCAACGCAC TTTGAATTTT GTAATCCTGA AGGGATCGTA AAAACAGCTC TTCTTCAAAT CTATACATTA AGACGACTCG AAATCCACAT ATCAAATATC CGAGTGTAGT AAACATTCCA AAACCGTGAT GGAATGGGAC AACACTTAAA ATCGCAGTAT CCGGAACGAT TTGATTGCCA AAAATAGGAT CTCTGGCATG CGAGAATCTG ACGCAGGCAG TTCTATGCGG AAGGGCCACA CCCTTAGGTA ACCCAGTAGA TCCAGAGGAA TTCATTATCA GTGCAATTGT TTTGTCACGA TCAAAGGACT CTGGTACAAA ATCGTATTCA TTAAAACCGG GAGGTAGATG AGATGTGACG AACGTGTACA TCGACTGAAA TCCCTGGTAA TCCGTTTTAG AATCCATGAT AATAATTTTC TGGATTATTG GTAATTTTTT TTGCACGTTC AAAATTTTTT GCAACCCCTT TTTGGAAACA AACACTACGG TAGGCTGCGA AATGTTCATA CTGTTGAGCA ATTCACGTTC ATTATAAATG TCGTTCGCGG GCGCAACTGC AACTCCGATA AATAACGCGC CCAACACCGG CATAAAGAAT TGAAGAGAGT TTTCACTGCA TACGACGATT CTGTGATTTG TATTCAGCCC ATATCGTTTC ATAGCTTCTG CCAACCGAAC GGACATTTCG AAGTATTCCG CGTACGTGAT GTTCACCTCG ATATGTGCAT CTGTAAAAGC AATTGTTCCA GGAACCAGGG CGTATCTCTT CATAGCCTTA TGCAGTTGCT CTCCAGCGGT TCCATCCTCT AGAGGATAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT GGTGGATCCA CGGTTCACTA AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGACCCCGT AATTGATTAC TATTAATAAC TAAGATCTGG TACCTTGAAT TCATGCTTCT CCTCCCTTTA GTGAGGGTAA TTCTCTCTCT CTCCCTATAG TGAGTCGTAT TAATTCCTTC TCTTCTATAG TGTCACCTAA ATCGTTGCAA TTCGTAATCA TGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA AAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGATTGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGA GTCGGTGGGC CTCGGGGGCG GGTGCGGGGT CGGCGGGGCC GCCCCGGGTG GCTTCGGTCG GAGCCATGGG GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG GGGAGGTTGC GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGGTCGACGG TGTGGCGCGT GGCGGGGTAG TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCGGGGGA CGTCGTCGCG GGTGGCGAGG CGCACCGTGG GCTTGTACTC GGTCATGGAA GGTCGTCTCC TTGTGAGGGG TCAGGGGCGT GGGTCAGGGG ATGGTGGCGG CACCGGTCGT GGCGGCCGAC CTGCAGGCAT GCAAGCTAGC TTTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCA ATCTTTCGTC
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