pJTU1278
$128.00
In stock
SKU
PL82756
100μg liquid plasmid(Instantly):$149
| name | pJTU1278 | another name | apJTU1278 |
| Insert Size (bp) | Plasmid host | Wide host | |
| Plasmid uses | Fragment type | ||
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Tsr | Fluorescent labeling | |
| Promoter | Replicon | pIJ101pBR322RP4T | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | M13R：CAGGAAACAGCTATGACC | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTGGGGGGGC AGGAGCCCCG CGGGGGGTGG TCGGGTCAGG CGCCGAACCG GCGGGCGGCG GCGGCGACCA GGCCGTCGGC GGCGGCCATG GCGCGGTCGC GGTCGGTGGT GAGGGCGGTG CGGTCGGCGG CGGCCCAGTC CTGCTCTCAC GCAACGTCTA CGTGGACGCT CAGGGCGACA CGATCGAGGT CGGGGAGTCC GTCGCTCCGG CGGGCCGCTG GCGCGTCCAC CGGGACTGAT CAAGGCGAAT ACTTCATATG CGGGGATCGA CCGCGCGGGT CCCGGACGGG GAAGAGCGGG GAGCTTTGCC AGAGAGCGAC GACTTCCCCT TGCGTTGGTG ATTGCCGGTC AGGGCAGCCA TCCGCCATCG TCGCGTAGGG TGTCACACCC CAGGAATCGC GTCACTGAAC ACAGCAGCCG GTAGGACGAC CATGACTGAG TTGGACACCA TCGCAAATCC GTCCGATCCC GCGGTGCAGC GGATCATCGA TGTCACCAAG CCGTCGCGAT CCAACATAAA GACAACGTTG ATCGAGGACG TCGAGCCCCT CATGCACAGC ATCGCGGCCG GGGTGGAGTT CATCGAGGTC TACGGCAGCG ACAGCAGTCC TTTTCCATCT GAGTTGCTGG ATCTGTGCGG GCGGCAGAAC ATACCGGTCC GCCTCATCGA CTCCTCGATC GTCAACCAGT TGTTCAAGGG GGAGCGGAAG GCCAAGACAT TCGGCATCGC CCGCGTCCCT CGCCCGGCCA GGTTCGGCGA TATCGCGAGC CGGCGTGGGG ACGTCGTCGT TCTCGACGGG GTGAAGATCG TCGGGAACAT CGGCGCGATA GTACGCACGT CGCTCGCGCT CGGAGCGTCG GGGATCATCC TGGTCGACAG TGACATCACC AGCATCGCGG ACCGGCGTCT CCAAAGGGCC AGCCGAGGTT ACGTCTTCTC CCTTCCCGTC GTTCTCTCCG GTCGCGAGGA GGCCATCGCC TTCATTCGGG ACAGCGGTAT GCAGCTGATG ACGCTCAAGG CGGATGGCGA CATTTCCGTG AAGGAACTCG GGGACAATCC GGATCGGCTG GCCTTGCTGT TCGGCAGCGA AAAGGGTGGG CCTTCCGACC TGTTCGAGGA GGCGTCTTCC GCCTCGGTTT CCATCCCCAT GATGAGCCAG ACCGAGTCTC TCAACGTTTC CGTTTCCCTC GGAATCGCGC TGCACGAGAG GATCGACAGG AATCTCGCGG CCAACCGATA AGCGCCTCTG TTCCTCGGAC GCTCGGTTCC TCGACCTCGA TTCGTCAGTG ATGATCAGAC GTCGAAGGTG TAGTGCCGGG TGTGGTCCAG CAGGGCCGCC GGGGTGGTGT CGTTCCACGG CTTCATCGTC TCGTTGAGGT CGACCAAAGC GGCCATCGTG CCTCCCCACT CCTGCAGATC CCTGCAGGTC GACGGATCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GATCCCCGGG GACCTGCAGG TCGACTCTAG CTAGACACGT CTGAAGCTAG CTTCGAGGAA CTTTCGGCGG CTTTGCTGTG CGACAGGCTC ACGTCTAAAA GGAAATAAAT CATGGGTCAT AAAATTATCA CGTTGTCCGG CGCGGCGACG GATGTTCTGT ATGCGCTGTT TTTCCGTGGC GCGTTGCTGT CTGGTGATCT GCCTTCTAAA TCTGGCACAG CCGAATTGCG CGAGCTTGGT TTTGCTGAAA CCAGACACAC AGCAACTGAA TACCAGAAAG AAAATCACTT TACCTTTCTG ACATCAGAAG GGCAGAAATT TGCCGTTGAA CACCTGGTCA ATACGCGTTT TGGTGAGCAG CAATATTGCG CTTCGATGAC GCTTGGCGTT GAGATTGATA CCTCTGCTGC ACAAAAGGCA ATCGACGAGC TGGACCAGCG CATTCGTGAC ACCGTCTCCT TCGAACTTAT TCGCAATGGA GTGTCATTCA TCAAGGACGC CGCTATCGCA AATGGTGCTA TCCACGCAGC GGCAATCGAA ACACCTCAGC CGGTGACCAA TATCTACAAC ATCAGCCTTG GTATCCAGCG TGATGAGCCA GCGCAGAACA AGGTAACCGT CAGTGCCGAT AAGTTCAAAG TTAAACCTGG TGTTGATACC AACATTGAAA CGTTGATCGA AAACGCGCTG AAAAACGCTG CTGAATGTGC GGCGCTGGAT GTCACAAAGC AAATGGCAGC AGACAAGAAA GCGATGGATG AACTGGCTTC CTATGTCCGC ACGGCCATCA TGATGGAATG TTTCCCCGGT GGTGTTATCT GGCAGCAGTG CCGTCGATAG TATGCAATTG ATAATTATTA TCATTTGCGG GTCCTTTCCG GCGATCCGCC TTGTTACGGG GCGGCGACCT CGCGGGTTTT CGCTATTTAT GAAAATTTTC CGGTTTAAGG CGTTTCCGTT CTTCTTCGTC ATAACTTAAT GTTTTTATTT AAAATACCCT CTGAAAAGAA AGGAAACGAC AGGTGCTGAA AGCGAGCTTT TTGGCCTCTG TCGTTTCCTT TCTCTGTTTT TGTCCGTGGA ATGAACAATG GAAGTCAACA AAAAGCAGAG CTTATCGATG ATAAGCGGTC AAACATGAGA ATTGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTCCA CCGCGGTGGC GGCCGCTCTA GAACTAGTGG ATCCCCCGGG CTGCAGGAAT TCGATATCAA GCTTATCGAT ACCGTCGACC TCGAGGGGGG GCCCGGTACC CAATTCGCCC TATAGTGAGT CGTATTACGC GCGCTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTCAGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTCCCGCAC GATCAGCGTC CCGCCGACCA GGAGCAGTGC GGCCAGCACT GCCGCTAACG CCTGGTCCTG GTCCCGGTCC TGGTGGTGCA TCAGTCCTCC CCGTGATCAC TTCGGCACCC ACCGTAGTGA TCACCCCCGA CAGCGGATCA AGGGGTTTGC GGGTCCCGGT CGGCGCCGGG CGGGGGAGGC AGGAGCCGCC GACGCTGCCT CTGGGACGGG CCGGACGGCA GGGGGACCGG CGGCCGGGCG AGCTGCAGCC GGGGGTCCGG CAGGGCCGGA GCGGGCGGAA CCGTGCTCTG ACCTGCGGCC CGAGTTTCGT CACGTGACGG AATGGAAGGC TGCTGCATTT CGTCACGTGA CGTATCTCGG CGAGCGACTG CCGACGCCAC GGCGGACACG ATCGCCTCGC GCTGGCGCCG GGCCTCGTAC GCCCGCTGGC GGCAGGAGCG GCGGCAGTAG TCCCGGCTCC GGCCGACGCC GGATTGCTTG ATCTCCGAGC CGCACCAGGC GCAGAGCTTC GCGCCGTCGG CGTCCCTGGG GGTGGTGGTG CTCATGGCCG ACGACCGTAC GCGGCACGTC TCGTAGCGAG GCGAGTCGGG CGCGACGCCT GCACGAAGTG CCGGCGGGGC CGACCCCGGG CGAGTAATCC CAGGATTACT CCCGCGGCTT CGACCCCGGC CGCCGTCGCC GCGTACGTCA CCGACCCCCG CCGTACGTCA CCGGGATGAC GTACGGCGGG GGGGAGCGAG TTAGTGCGAA GTGGGCCCAC TTGCGAGCCG GGCGATGTGC CGGGCGGCCC GCTCCTGGCG GTCGTCGGCG TCGTCGTCCT GGTCGTCGTC CTGCTCTCGC CGTCGGCGTG CAGTTGCTTC CTCGCGGCGC TGGGCGAGGG CGGCGAGCAT GTCGGCGTAC GCCTCGGCCA CCTCCCCCGC CGTGAGCACC ACCACTGTGT CGGCCGCGTC GGCCAGCGCC AGGACCTCCC GCACCCGTTC GCCCACGGCC GCCGAATCCT CGTTGCCGTC CTTGCCTTCG GCGGCCCGGG TCGCCTCGAG GTCGAGGGCG CGGCGGGTGA CCGCGTGCCA TCCGTCCTCG GTCACGGCGA CCCCGGCCCG CAGCTCCCCG CCGTCGGCGT CGGCCGCCAG GAGCAGATCG AGGTCGTCGG CCTCGGTGTC GCCGCCGTCG AGCCCGAGCA TCTGCCGCAG GTAGCGGGTC CATTCGATGG CCCGGCGTCC CCGGGTTGCC CGCTCGTACT CGTGCCAGCG CGAGAGGTTC CACTCCAGCG AGCCGACCCC GGCGGCGTCG TCCTCGGTCA TGCCGCCGGT CAGGTCCCCG ATCCGTCCGA GGAGTTCGAA CGGGGCGACG TTCCCGCCGG TCGCCGTCTT GAGGTCGGCG CGGGCGAGTT CGAGGGCGGG CGCCTTCCCG TCCTGGGTCT TGGCGATGTA CTCGGCGAGG TCGTTGGCGT CGCGCTCGGT CTCCAGCCGC TTGAAGTCGA CGCCGTGCCG GTCGTCGGGC GTGAAGGCGG GGTTGACCTT GCGCAGGGCG GCGGTCCACA CGGACCGCCA GTGCCCCTGC CACTCGTCGA GCGCGGCGCC GGTCGGCTCG AAGGTGGCGA CGATCTGCTT CGCGGACCGC TCCCCCTCGG TCCGGCCGCC GACCAGGACG ATCGCGTGGA TGTGCGGGTG CCAGCCGTTG ATCTGCCCCA CGGTGACTTC GGTCGCGCGG ATCATGCCGA CGTACCCGAT CCGGTCTCGG ATGCCCTCGC GGTCGGCGGC CCGGTGCCCG TCCTTGGCCC GGCGTCCGGC CCACGTGCCG CCCGTGATCA GTCGCTGGTA GGCGCCCGGC CGCCGGGGGC TGTCCGGCGT CTTCCGGGTG CCCTGGAGGG CGTCCATGAG GTCCGCGAGC CGGTCCGTGT GCCCATGGCG GGCCGTGAAG GTGACCAGGT AGGCGGTCCC CCCGCGCTTG ATCCACTCGA CCACGGCGGC GGTGATCTCC TCGGCCCGCT TGTGCCGGAT CGTGGCGGCG CAGACCGGGC AGAGCCAGAT CCGCCCGCAC CGCATCAGGC CCAGGACCAC GGACGTTCCG GCCGCCGTCT GGGCGACGAT CACGCCGGAG GCAGGGTCCA TCAGGGCGCG GCCGCAGCCC TTGCACGCGG CGTCCCCGCT GATCCGCCAC AGCGTCCGGC GGCGGCTGTA CCGGGCGGCT TTCCGCAGTC GGGCAGCGTC GCTCCGCGAC GTGCTTCCTA CTTCCGAGAG GCTGTCGCCT CTCGGGCTCT CCCCATCCAC CCCGTCCGGA GAAACCGCAG GTCGGAGGGG TGCGGGAAAC TCTGTTGTTT CTTTCCCAAG GTGTTCGCTT TTGCCTCGGG CGGCATCTCG CGTCACACGC GCGATCGCCC GCTTCGCTGC CATCCGGCAG CGGTCTGAGC AGTAGATACG CGGCCGTTTG CCCGGTGTGT GGGCAATTGC GGTCCCGCAG TGGCAGCGGG GCCCGGCGGG CCGATCTGGC AATGCCTCGG CATCGCTCCG TACTCTGGGC ACGAGCAACG TTCCTGTCTC GCCCGGCTAA GGGGCGCGAG TCTGGGAGCG GACGGGTCGG AGGTGCGAAG TCCGGCCCGT TGCTCTTTGG TCTGGTGGGA ATCCTGGCAC CAATCGGGCC AGAGGTTCCC TCCGCCACTC CCGACGCCCC TTGGGGCTGG TGTGACTTGG AGGGCCGAAG AGAGCCCCGC CGGTGATCCG GCGGGGCTTT GACGTGCGGT CAGTGCGTGT GTCGGCGAAC GATGGCCACA AAGCCCTGGA AGCCGAGCGG TCCGGCAAAG TCGGCCCAGT CCAACCGGGC TCAGCGC
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