pEarleyGate 100
$99.00
In stock
SKU
PL82754
pEarleyGate100
100μg liquid plasmid(Instantly):$120
| name | pEarleyGate 100 | another name | apEarleyGate 100 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Bar | Fluorescent labeling | |
| Promoter | 35S | Replicon | pVS1 |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGCAGGATA TATTGTGGTG TAAACAAATT GACGCTTAGA CAACTTAATA ACACATTGCG GACGTTTTTA ATGTACTGAA TTAACGCCGA ATTAATTCGA GCTCGGATCT GATAATTTAT TTGAAAATTC ATAAGAAAAG CAAACGTTAC ATGAATTGAT GAAACAATAC AAAGACAGAT AAAGCCACGC ACATTTAGGA TATTGGCCGA GATTACTGAA TATTGAGTAA GATCACGGAA TTTCTGACAG GAGCATGTCT TCAATTCAGC CCAAATGGCA GTTGAAATAC TCAAACCGCC CCATATGCAG GAGCGGATCA TTCATTGTTT GTTTGGTTGC CTTTGCCAAC ATGGGAGTCC AAGATTCTGC AGTCAAATCT CGGTGACGGG CAGGACCGGA CGGGGCGGTA CCGGCAGGCT GAAGTCCAGC TGCCAGAAAC CCACGTCATG CCAGTTCCCG TGCTTGAAGC CGGCCGCCCG CAGCATGCCG CGGGGGGCAT ATCCGAGCGC CTCGTGCATG CGCACGCTCG GGTCGTTGGG CAGCCCGATG ACAGCGACCA CGCTCTTGAA GCCCTGTGCC TCCAGGGACT TCAGCAGGTG GGTGTAGAGC GTGGAGCCCA GTCCCGTCCG CTGGTGGCGG GGGGAGACGT ACACGGTCGA CTCGGCCGTC CAGTCGTAGG CGTTGCGTGC CTTCCAGGGG CCCGCGTAGG CGATGCCGGC GACCTCGCCG TCCACCTCGG CGACGAGCCA GGGATAGCGC TCCCGCAGAC GGACGAGGTC GTCCGTCCAC TCCTGCGGTT CCTGCGGCTC GGTACGGAAG TTGACCGTGC TTGTCTCGAT GTAGTGGTTG ACGATGGTGC AGACCGCCGG CATGTCCGCC TCGGTGGCAC GGCGGATGTC GGCCGGGCGT CGTTCTGGGC TCATCGATTC GATTTGGTGT ATCGAGATTG GTTATGAAAT TCAGATGCTA GTGTAATGTA TTGGTAATTT GGGAAGATAT AATAGGAAGC AAGGCTATTT ATCCATTTCT GAAAAGGCGA AATGGCGTCA CCGCGAGCGT CACGCGCATT CCGTTCTTGC TGTAAAGCGT TGTTTGGTAC ACTTTTGACT AGCGAGGCTT GGCGTGTCAG CGTATCTATT CAAAAGTCGT TAATGGCTGC GGATCAAGAA AAAGTTGGAA TAGAAACAGA ATACCCGCGA AATTCAGGCC CGGTTGCCAT GTCCTACACG CCGAAATAAA CGACCAAATT AGTAGAAAAA TAAAAACTGA CTCGGATACT TACGTCACGT CTTGCGCACT GATTTGAAAA ATCTCAGAAT TCCAATCCCA CAAAAATCTG AGCTTAACAG CACAGTTGCT CCTCTCAGAG CAGAATCGGG TATTCAACAC CCTCATATCA ACTACTACGT TGTGTATAAC GGTCCACATG CCGGTATATA CGATGACTGG GGTTGTACAA AGGCGGCAAC AAACGGCGTT CCCGGAGTTG CACACAAGAA ATTTGCCACT ATTACAGAGG CAAGAGCAGC AGCTGACGCG TACACAACAA GTCAGCAAAC AGACAGGTTG AACTTCATCC CCAAAGGAGA AGCTCAACTC AAGCCCAAGA GCTTTGCTAA GGCCCTAACA AGCCCACCAA AGCAAAAAGC CCACTGGCTC ACGCTAGGAA CCAAAAGGCC CAGCAGTGAT CCAGCCCCAA AAGAGATCTC CTTTGCCCCG GAGATTACAA TGGACGATTT CCTCTATCTT TACGATCTAG GAAGGAAGTT CGAAGGTGAA GGTGACGACA CTATGTTCAC CACTGATAAT GAGAAGGTTA GCCTCTTCAA TTTCAGAAAG AATGCTGACC CACAGATGGT TAGAGAGGCC TACGCAGCAG GTCTCATCAA GACGATCTAC CCGAGTAACA ATCTCCAGGA GATCAAATAC CTTCCCAAGA AGGTTAAAGA TGCAGTCAAA AGATTCAGGA CTAATTGCAT CAAGAACACA GAGAAAGACA TATTTCTCAA GATCAGAAGT ACTATTCCAG TATGGACGAT TCAAGGCTTG CTTCATAAAC CAAGGCAAGT AATAGAGATT GGAGTCTCTA AAAAGGTAGT TCCTACTGAA TCTAAGGCCA TGCATGGAGT CTAAGATTCA AATCGAGGAT CTAACAGAAC TCGCCGTGAA GACTGGCGAA CAGTTCATAC AGAGTCTTTT ACGACTCAAT GACAAGAAGA AAATCTTCGT CAACATGGTG GAGCACGACA CTCTGGTCTA CTCCAAAAAT GTCAAAGATA CAGTCTCAGA AGACCAAAGG GCTATTGAGA CTTTTCAACA AAGGATAATT TCGGGAAACC TCCTCGGATT CCATTGCCCA GCTATCTGTC ACTTCATCGA AAGGACAGTA GAAAAGGAAG GTGGCTCCTA CAAATGCCAT CATTGCGATA AAGGAAAGGC TATCATTCAA GATCTCTCTG CCGACAGTGG TCCCAAAGAT GGACCCCCAC CCACGAGGAG CATCGTGGAA AAAGAAGACG TTCCAACCAC GTCTTCAAAG CAAGTGGATT GATGTGACAT CTCCACTGAC GTAAGGGATG ACGCACAATC CCACTATCCT TCGCAAGACC CTTCCTCTAT ATAAGGAAGT TCATTTCATT TGGAGAGGAC ACGCTCGAGA TCACAAGTTT GTACAAAAAA GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CATATTGGCG GCCGCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT TTGAGTTAGG ATCCGTCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA TCATGCCGTT TGTGATGGCT TCCATGTCGG CAGAATGCTT AATGAATTAC AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAAACGCG TGGATCCGGC TTACTAAAAG CCAGATAACA GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA TATACTGATA TGTATACCCG AAGTATGTCA AAAAGAGGTA TGCTATGAAG CAGCGTATTA CAGTGACAGT TGACAGCGAC AGCTATCAGT TGCTCAAGGC ATATATGATG TCAATATCTC CGGTCTGGTA AGCACAACCA TGCAGAATGA AGCCCGTCGT CTGCGTGCCG AACGCTGGAA AGCGGAAAAT CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT ATTGAAATGA ACGGCTCTTT TGCTGACGAG AACAGGGGCT GGTGAAATGC AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG TCTGTTTGTG GATGTACAGA GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC CCTGGCCAGT GCACGTCTGC TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA TATCGGGGAT GAAAGCTGGC GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA CACAGCCAGT CTGCAGGTCG ACCATAGTGA CTGGATATGT TGTGTTTTAC AGCATTATGT AGTCTGTTTT TTATGCAAAA TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAGCT TTCTTGTACA AAGTGGTGCC TAGGTGAGTC TAGAGAGTTA ATTAAGACCC GGGACTAGTC CCTAGAGTCC TGCTTTAATG AGATATGCGA GACGCCTATG ATCGCATGAT ATTTGCTTTC AATTCTGTTG TGCACGTTGT AAAAAACCTG AGCATGTGTA GCTCAGATCC TTACCGCCGG TTTCGGTTCA TTCTAATGAA TATATCACCC GTTACTATCG TATTTTTATG AATAATATTC TCCGTTCAAT TTACTGATTG TACCCTACTA CTTATATGTA CAATATTAAA ATGAAAACAA TATATTGTGC TGAATAGGTT TATAGCGACA TCTATGATAG AGCGCCACAA TAACAAACAA TTGCGTTTTA TTATTACAAA TCCAATTTTA AAAAAAGCGG CAGAACCGGT CAAACCTAAA AGACTGATTA CATAAATCTT ATTCAAATTT CAAAAGTGCC CCAGGGGCTA GTATCTACGA CACACCGAGC GGCGAACTAA TAACGCTCAC TGAAGGGAAC TCCGGTTCCC CGCCGGCGCG CATGGGTGAG ATTCCTTGAA GTTGAGTATT GGCCGTCCGC TCTACCGAAA GTTACGGGCA CCATTCAACC CGGTCCAGCA CGGCGGCCGG GTAACCGACT TGCTGCCCCG AGAATTATGC AGCATTTTTT TGGTGTATGT GGGCCCCAAA TGAAGTGCAG GTCAAACCTT GACAGTGACG ACAAATCGTT GGGCGGGTCC AGGGCGAATT TTGCGACAAC ATGTCGAGGC TCAGCAGGAC CTGCAGGCAT GCAAGCTTGG CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGCTA GAGCAGCTTG AGCTTGGATC AGATTGTCGT TTCCCGCCTT CAGTTTAAAC TATCAGTGTT TGACAGGATA TATTGGCGGG TAAACCTAAG AGAAAAGAGC GTTTATTAGA ATAACGGATA TTTAAAAGGG CGTGAAAAGG TTTATCCGTT CGTCCATTTG TATGTGCATG CCAACCACAG GGTTCCCCTC GGGATCAAAG TACTTTGATC CAACCCCTCC GCTGCTATAG TGCAGTCGGC TTCTGACGTT CAGTGCAGCC GTCTTCTGAA AACGACATGT CGCACAAGTC CTAAGTTACG CGACAGGCTG CCGCCCTGCC CTTTTCCTGG CGTTTTCTTG TCGCGTGTTT TAGTCGCATA AAGTAGAATA CTTGCGACTA GAACCGGAGA CATTACGCCA TGAACAAGAG CGCCGCCGCT GGCCTGCTGG GCTATGCCCG CGTCAGCACC GACGACCAGG ACTTGACCAA CCAACGGGCC GAACTGCACG CGGCCGGCTG CACCAAGCTG TTTTCCGAGA AGATCACCGG CACCAGGCGC GACCGCCCGG AGCTGGCCAG GATGCTTGAC CACCTACGCC CTGGCGACGT TGTGACAGTG ACCAGGCTAG ACCGCCTGGC CCGCAGCACC CGCGACCTAC TGGACATTGC CGAGCGCATC CAGGAGGCCG GCGCGGGCCT GCGTAGCCTG GCAGAGCCGT GGGCCGACAC CACCACGCCG GCCGGCCGCA TGGTGTTGAC CGTGTTCGCC GGCATTGCCG AGTTCGAGCG TTCCCTAATC ATCGACCGCA CCCGGAGCGG GCGCGAGGCC GCCAAGGCCC GAGGCGTGAA GTTTGGCCCC CGCCCTACCC TCACCCCGGC ACAGATCGCG CACGCCCGCG AGCTGATCGA CCAGGAAGGC CGCACCGTGA AAGAGGCGGC TGCACTGCTT GGCGTGCATC GCTCGACCCT GTACCGCGCA CTTGAGCGCA GCGAGGAAGT GACGCCCACC GAGGCCAGGC GGCGCGGTGC CTTCCGTGAG GACGCATTGA CCGAGGCCGA CGCCCTGGCG GCCGCCGAGA ATGAACGCCA AGAGGAACAA GCATGAAACC GCACCAGGAC GGCCAGGACG AACCGTTTTT CATTACCGAA GAGATCGAGG CGGAGATGAT CGCGGCCGGG TACGTGTTCG AGCCGCCCGC GCACGTCTCA ACCGTGCGGC TGCATGAAAT CCTGGCCGGT TTGTCTGATG CCAAGCTGGC GGCCTGGCCG GCCAGCTTGG CCGCTGAAGA AACCGAGCGC CGCCGTCTAA AAAGGTGATG TGTATTTGAG TAAAACAGCT TGCGTCATGC GGTCGCTGCG TATATGATGC GATGAGTAAA TAAACAAATA CGCAAGGGGA ACGCATGAAG GTTATCGCTG TACTTAACCA GAAAGGCGGG TCAGGCAAGA CGACCATCGC AACCCATCTA GCCCGCGCCC TGCAACTCGC CGGGGCCGAT GTTCTGTTAG TCGATTCCGA TCCCCAGGGC AGTGCCCGCG ATTGGGCGGC CGTGCGGGAA GATCAACCGC TAACCGTTGT CGGCATCGAC CGCCCGACGA TTGACCGCGA CGTGAAGGCC ATCGGCCGGC GCGACTTCGT AGTGATCGAC GGAGCGCCCC AGGCGGCGGA CTTGGCTGTG TCCGCGATCA AGGCAGCCGA CTTCGTGCTG ATTCCGGTGC AGCCAAGCCC TTACGACATA TGGGCCACCG CCGACCTGGT GGAGCTGGTT AAGCAGCGCA TTGAGGTCAC GGATGGAAGG CTACAAGCGG CCTTTGTCGT GTCGCGGGCG ATCAAAGGCA CGCGCATCGG CGGTGAGGTT GCCGAGGCGC TGGCCGGGTA CGAGCTGCCC ATTCTTGAGT CCCGTATCAC GCAGCGCGTG AGCTACCCAG GCACTGCCGC CGCCGGCACA ACCGTTCTTG AATCAGAACC CGAGGGCGAC GCTGCCCGCG AGGTCCAGGC GCTGGCCGCT GAAATTAAAT CAAAACTCAT TTGAGTTAAT GAGGTAAAGA GAAAATGAGC AAAAGCACAA ACACGCTAAG TGCCGGCCGT CCGAGCGCAC GCAGCAGCAA GGCTGCAACG TTGGCCAGCC TGGCAGACAC GCCAGCCATG AAGCGGGTCA ACTTTCAGTT GCCGGCGGAG GATCACACCA AGCTGAAGAT GTACGCGGTA CGCCAAGGCA AGACCATTAC CGAGCTGCTA TCTGAATACA TCGCGCAGCT ACCAGAGTAA ATGAGCAAAT GAATAAATGA GTAGATGAAT TTTAGCGGCT AAAGGAGGCG GCATGGAAAA TCAAGAACAA CCAGGCACCG ACGCCGTGGA ATGCCCCATG TGTGGAGGAA CGGGCGGTTG GCCAGGCGTA AGCGGCTGGG TTGTCTGCCG GCCCTGCAAT GGCACTGGAA CCCCCAAGCC CGAGGAATCG GCGTGAGCGG TCGCAAACCA TCCGGCCCGG TACAAATCGG CGCGGCGCTG GGTGATGACC TGGTGGAGAA GTTGAAGGCC GCGCAGGCCG CCCAGCGGCA ACGCATCGAG GCAGAAGCAC GCCCCGGTGA ATCGTGGCAA GCGGCCGCTG ATCGAATCCG CAAAGAATCC CGGCAACCGC CGGCAGCCGG TGCGCCGTCG ATTAGGAAGC CGCCCAAGGG CGACGAGCAA CCAGATTTTT TCGTTCCGAT GCTCTATGAC GTGGGCACCC GCGATAGTCG CAGCATCATG GACGTGGCCG TTTTCCGTCT GTCGAAGCGT GACCGACGAG CTGGCGAGGT GATCCGCTAC GAGCTTCCAG ACGGGCACGT AGAGGTTTCC GCAGGGCCGG CCGGCATGGC CAGTGTGTGG GATTACGACC TGGTACTGAT GGCGGTTTCC CATCTAACCG AATCCATGAA CCGATACCGG GAAGGGAAGG GAGACAAGCC CGGCCGCGTG TTCCGTCCAC ACGTTGCGGA CGTACTCAAG TTCTGCCGGC GAGCCGATGG CGGAAAGCAG AAAGACGACC TGGTAGAAAC CTGCATTCGG TTAAACACCA CGCACGTTGC CATGCAGCGT ACGAAGAAGG CCAAGAACGG CCGCCTGGTG ACGGTATCCG AGGGTGAAGC CTTGATTAGC CGCTACAAGA TCGTAAAGAG CGAAACCGGG CGGCCGGAGT ACATCGAGAT CGAGCTAGCT GATTGGATGT ACCGCGAGAT CACAGAAGGC AAGAACCCGG ACGTGCTGAC GGTTCACCCC GATTACTTTT TGATCGATCC CGGCATCGGC CGTTTTCTCT ACCGCCTGGC ACGCCGCGCC GCAGGCAAGG CAGAAGCCAG ATGGTTGTTC AAGACGATCT ACGAACGCAG TGGCAGCGCC GGAGAGTTCA AGAAGTTCTG TTTCACCGTG CGCAAGCTGA TCGGGTCAAA TGACCTGCCG GAGTACGATT TGAAGGAGGA GGCGGGGCAG GCTGGCCCGA TCCTAGTCAT GCGCTACCGC AACCTGATCG AGGGCGAAGC ATCCGCCGGT TCCTAATGTA CGGAGCAGAT GCTAGGGCAA ATTGCCCTAG CAGGGGAAAA AGGTCGAAAA GGTCTCTTTC CTGTGGATAG CACGTACATT GGGAACCCAA AGCCGTACAT TGGGAACCGG AACCCGTACA TTGGGAACCC AAAGCCGTAC ATTGGGAACC GGTCACACAT GTAAGTGACT GATATAAAAG AGAAAAAAGG CGATTTTTCC GCCTAAAACT CTTTAAAACT TATTAAAACT CTTAAAACCC GCCTGGCCTG TGCATAACTG TCTGGCCAGC GCACAGCCGA AGAGCTGCAA AAAGCGCCTA CCCTTCGGTC GCTGCGCTCC CTACGCCCCG CCGCTTCGCG TCGGCCTATC GCGGCCGCTG GCCGCTCAAA AATGGCTGGC CTACGGCCAG GCAATCTACC AGGGCGCGGA CAAGCCGCGC CGTCGCCACT CGACCGCCGG CGCCCACATC AAGGCACCCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGC ATTCTAGGTA CTAAAACAAT TCATCCAGTA AAATATAATA TTTTATTTTC TCCCAATCAG GCTTGATCCC CAGTAAGTCA AAAAATAGCT CGACATACTG TTCTTCCCCG ATATCCTCCC TGATCGACCG GACGCAGAAG GCAATGTCAT ACCACTTGTC CGCCCTGCCG CTTCTCCCAA GATCAATAAA GCCACTTACT TTGCCATCTT TCACAAAGAT GTTGCTGTCT CCCAGGTCGC CGTGGGAAAA GACAAGTTCC TCTTCGGGCT TTTCCGTCTT TAAAAAATCA TACAGCTCGC GCGGATCTTT AAATGGAGTG TCTTCTTCCC AGTTTTCGCA ATCCACATCG GCCAGATCGT TATTCAGTAA GTAATCCAAT TCGGCTAAGC GGCTGTCTAA GCTATTCGTA TAGGGACAAT CCGATATGTC GATGGAGTGA AAGAGCCTGA TGCACTCCGC ATACAGCTCG ATAATCTTTT CAGGGCTTTG TTCATCTTCA TACTCTTCCG AGCAAAGGAC GCCATCGGCC TCACTCATGA GCAGATTGCT CCAGCCATCA TGCCGTTCAA AGTGCAGGAC CTTTGGAACA GGCAGCTTTC CTTCCAGCCA TAGCATCATG TCCTTTTCCC GTTCCACATC ATAGGTGGTC CCTTTATACC GGCTGTCCGT CATTTTTAAA TATAGGTTTT CATTTTCTCC CACCAGCTTA TATACCTTAG CAGGAGACAT TCCTTCCGTA TCTTTTACGC AGCGGTATTT TTCGATCAGT TTTTTCAATT CCGGTGATAT TCTCATTTTA GCCATTTATT ATTTCCTTCC TCTTTTCTAC AGTATTTAAA GATACCCCAA GAAGCTAATT ATAACAAGAC GAACTCCAAT TCACTGTTCC TTGCATTCTA AAACCTTAAA TACCAGAAAA CAGCTTTTTC AAAGTTGTTT TCAAAGTTGG CGTATAACAT AGTATCGACG GAGCCGATTT TGAAACCGCG GTGATCACAG GCAGCAACGC TCTGTCATCG TTACAATCAA CATGCTACCC TCCGCGAGAT CATCCGTGTT TCAAACCCGG CAGCTTAGTT GCCGTTCTTC CGAATAGCAT CGGTAACATG AGCAAAGTCT GCCGCCTTAC AACGGCTCTC CCGCTGACGC CGTCCCGGAC TGATGGGCTG CCTGTATCGA GTGGTGATTT TGTGCCGAGC TGCCGGTCGG GGAGCTGTTG GCTGGCTGG
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