pKJE7
$99.00
In stock
SKU
PL82739
分子伴侣质粒
100μg liquid plasmid(Instantly):$120
| name | pKJE7 | another name | apKJE7 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | P0487-F：GGTAGCTCAGAGAACCTTCG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AATAAGTGAT AATAAGCGGA TGAATGGCAG AAATTCGAAG AATAGTTACG GCTTATGACA TCTTTGTGGA CACATCATTC ACTTTTTATT CACATCCGGC CCTGAACTCG CTAGGACTTG CCCCGGTGCA TTTTTTAAAT ACCCGCGAAA AATAGAGCTG ATCGTCAAAT CCAACATTGC GCCCAACGGT CGCTATCGGC ATTCGCGTAG TGCTAAGCAG AAGTTTCGCC TGGCTGATAC GCTGATCTTC GCGCCAGCTC AATACGCTAA TGCCTAACTG CTGGCGGAAC AGATGTGATA ACCGGGAGGG CGACAGGCAG ACATGCTGGG CGACGCTGGC GATATCAAAA TGGCTGTCCG CCAGATGGTC GCTGATATAC TGGCAGGCAT CGCGCACACG GCTATCCATC GGCGGGTGCA ACGACTCATT AATTACCGCC ATACGTCTGA GCAACAACTG CTCCAGCAGA TTGATCGCCA GTAGCTCAGA ATAGCGACCT TCCCCTTGCC CGGCGCTGAT GATCTGCCCG AACAGTTCGC TGAAATGCGG CTGGCGCGCC TCGTCCGGGC GGAAAAATCC TGTCTGGGCA AAGATTGTCG GCCAGGTCAG CCACTCCTGC CAGTAGGCGC GAGGCCGGAA ATAAACCCAC TGGTGATACC ACTCGCTGGC GTCCGGATGC CGTCCATAGT GATGAATCTC GCCCGGCGGA AACAATAATA TATCGCCAGG CCGACAGACA AACTGCTCGC CATTATTATT AATGACGCCC TCTCCGCGGA TGGTCAGGTT AAGAATATAT CCCTTCATGC CCAACGGACG ATCGATAAAA AAATCCAGAT ATCCATTCGC TTCAATTGGC GTCAGCCCGG CGACCAGATG GGCATTAAAT GAATATCCCG GCAATAGCGG ATCATTTTGC GTTTCAGCCA TGATTTCTCT ACCCCCCGAT GTTCAGAGAA GAACCAAATT GTCCATATCG ACCAGGACGA CAGAGCTTCC GTCTCCGCAA GACTTTGCGC TTGATGAAAG CACGTATCAA CCCCGCTTGT GAAAAGCGCT TTGTAACAAA AGCGTACAGT TCAGGCGATA AAATTAAGTA ACAGAAGTGT CTATAACTAT GGCTGGAATG TCCACATTGA ATATTTGCAC AGCGTCACAC TTTGCAAAGC ATTAGCATTT TTGTCCATAA GATTAGCGGA TCCTGCCTGA CGGTTTTTGC CGCGACTCTC TATAATTTCT CCATACCTGT TTTTCTGGAT GGAGTAAGAC CATGGCTATG GATTGTGGAG ACGTTTAGAT GGGTAAAATA ATTGGTATCG ACCTGGGTAC TACCAACTCT TGTGTAGCGA TTATGGATGG CACCACTCCT CGCGTGCTGG AGAACGCCGA AGGCGATCGC ACCACGCCTT CTATCATTGC CTATACCCAG GATGGTGAAA CTCTAGTTGG TCAGCCGGCT AAACGTCAGG CAGTGACGAA CCCGCAAAAC ACTCTGTTTG CGATTAAACG CCTGATTGGT CGCCGCTTCC AGGACGAAGA AGTACAGCGT GATGTTTCCA TCATGCCGTT CAAAATTATT GCTGCTGATA ACGGCGACGC ATGGGTCGAA GTTAAAGGCC AGAAAATGGC ACCGCCGCAG ATTTCTGCTG AAGTGCTGAA AAAAATGAAG AAAACCGCTG AAGATTACCT GGGTGAACCG GTAACTGAAG CTGTTATCAC CGTACCGGCA TACTTTAACG ATGCTCAGCG TCAGGCAACC AAAGACGCAG GCCGTATCGC TGGTCTGGAA GTAAAACGTA TCATCAACGA ACCGACCGCA GCTGCGCTGG CTTACGGTCT GGACAAAGGC ACTGGCAACC GTACTATCGC GGTTTATGAC CTGGGTGGTG GTACTTTCGA TATTTCTATT ATCGAAATCG ACGAAGTTGA CGGCGAAAAA ACCTTCGAAG TTCTGGCAAC CAACGGTGAT ACCCACCTGG GGGGTGAAGA CTTCGACAGC CGTCTGATCA ACTATCTGGT TGAAGAATTC AAGAAAGATC AGGGCATTGA CCTGCGCAAC GATCCGCTGG CAATGCAGCG CCTGAAAGAA GCGGCAGAAA AAGCGAAAAT CGAACTGTCT TCCGCTCAGC AGACCGACGT TAACCTGCCA TACATCACTG CAGACGCGAC CGGTCCGAAA CACATGAACA TCAAAGTGAC TCGTGCGAAA CTGGAAAGCC TGGTTGAAGA TCTGGTAAAC CGTTCCATTG AGCCGCTGAA AGTTGCACTG CAGGACGCTG GCCTGTCCGT ATCTGATATC GACGACGTTA TCCTCGTTGG TGGTCAGACT CGTATGCCAA TGGTTCAGAA GAAAGTTGCT GAGTTCTTTG GTAAAGAGCC GCGTAAAGAC GTTAACCCGG ACGAAGCTGT AGCAATCGGT GCTGCTGTTC AGGGTGGTGT TCTGACTGGT GACGTAAAAG ACGTACTGCT GCTGGACGTT ACCCCGCTGT CTCTGGGTAT CGAAACCATG GGCGGTGTGA TGACGACGCT GATCGCGAAA AACACCACTA TCCCGACCAA GCACAGCCAG GTGTTCTCTA CCGCTGAAGA CAACCAGTCT GCGGTAACCA TCCATGTGCT GCAGGGTGAA CGTAAACGTG CGGCTGATAA CAAATCTCTG GGTCAGTTCA ACCTAGATGG TATCAACCCG GCACCGCGCG GCATGCCGCA GATCGAAGTT ACCTTCGATA TCGATGCTGA CGGTATCCTG CACGTTTCCG CGAAAGATAA AAACAGCGGT AAAGAGCAGA AGATCACCAT CAAGGCTTCT TCTGGTCTGA ACGAAGATGA AATCCAGAAA ATGGTACGCG ACGCAGAAGC TAACGCCGAA GCTGACCGTA AGTTTGAAGA GCTGGTACAG ACTCGCAACC AGGGCGACCA TCTGCTGCAC AGCACCCGTA AGCAGGTTGA AGAAGCAGGC GACAAACTGC CGGCTGACGA CAAAACTGCT ATCGAGTCTG CGCTGACTGC ACTGGAAACT GCTCTGAAAG GTGAAGACAA AGCCGCTATC GAAGCGAAAA TGCAGGAACT GGCACAGGTT TCCCAGAAAC TGATGGAAAT CGCCCAGCAG CAACATGCCC AGCAGCAGAC TGCCGGTGCT GATGCTTCTG CAAACAACGC GAAAGATGAC GATGTTGTCG ACGCTGAATT TGAAGAAGTC AAAGACAAAA AATAATCGCC CTATAAACGG GTAATTATAC TGACACGGGC GAAGGGGAAT TTCCTCCCCG CCCGTGCATT CATCTAGGGG CAATTTAAAA AAGATGGCTA AGCAAGATTA TTACGAGATT TTAGGCGTTT CCAAAACAGC GGAAGAGCGT GAAATCAGAA AGGCCTACAA ACGCCTGGCC ATGAAATACC ACCCGGACCG TAACCAGGGT GACAAAGAGG CCGAGGCGAA ATTTAAAGAG ATCAAGGAAG CTTATGAAGT TCTGACCGAC TCGCAAAAAC GTGCGGCATA CGATCAGTAT GGTCATGCTG CGTTTGAGCA AGGTGGCATG GGCGGCGGCG GTTCTGGCGG CGGCGCAGAC TTCAGCGATA TTTTTGGTGA CGTTTTCGGC GATATTTTTG GCGGCGGACG TGGTCGTCAA CGTGCGGCGC GCGGTGCTGA TTTACGCTAT AACATGGAGC TCACCCTCGA AGAAGCTGTA CGTGGCGTGA CCAAAGAGAT CCGCATTCCG ACTCTGGAAG AGTGTGACGT TTGCCACGGT AGCGGTGCAA AACCAGGTAC ACAGCCGCAG ACTTGTCCGA CCTGTCATGG TTCTGGTCAG GTGCAGATGC GCCAGGGATT CTTCGCTGTA CAGCAGACCT GTCCACACTG TCAGGGCCGC GGTACGCTGA TCAAAGATCC GTGCAACAAA TGTCATGGTC ATGGTCGTGT TGAGCGCAGC AAAACGCTGT CCGTTAAAAT CCCGGCAGGG GTGGACACTG GAGACCGCAT CCGTCTTGCG GGCGAAGGTG AAGCGGGCGA GCATGGCGCA CCGGCAGGCG ATCTGTACGT TCAGGTTCAG GTTAAACAGC ACCCGATTTT CGAGCGTGAA GGCAACAACC TGTATTGCGA AGTCCCGATC AACTTCGCTA TGGCGGCGCT GGGTGGCGAA ATCGAAGTAC CGACCCTTGA TGGTCGCGTC AAACTGAAAG TGCCTGGCGA AACCCAGACC GGTAAGCTAT TCCGTATGCG CGGTAAAGGC GTCAAGTCTG TCCGCGGTGG CGCGCAGGGT GATTTGCTGT GCCGCGTTGT CGTCGAAACA CCGGTAGGCC TGAACGAAAG GCAGAAACAG CTGCTGCAAG AGCTGCAAGA AAGCTTCGGT GGCCCAACCG GCGAGCACAA CAGCCCGCGC TCAAAGAGCT TCTTTGATGG TGTGAAGAAG TTTTTTGACG ACCTGACCCG CTAACCTCGC GGAGAAATTC ATGAGTAGTA AAGAACAGAA AACGCCTGAG GGGCAAGCCC CGGAAGAAAT TATCATGGAT CAGCACGAAG AGATTGAGGC AGTTGAGCCA GAAGCTTCTG CTGAGCAGGT GGATCCGCGC GATGAAAAAG TTGCGAATCT CGAAGCTCAG CTGGCTGAAG CCCAGACCCG TGAACGTGAC GGCATTTTGC GTGTAAAAGC CGAAATGGAA AACCTGCGTC GTCGTACTGA ACTGGATATT GAAAAAGCCC ACAAATTCGC GCTGGAGAAA TTCATCAACG AATTGCTGCC GGTGATTGAT AGCCTGGATC GTGCGCTGGA AGTGGCTGAT AAAGCTAACC CGGATATGTC TGCGATGGTT GAAGGCATTG AGCTGACGCT GAAGTCGATG CTGGATGTTG TGCGTAAGTT TGGCGTTGAA GTGATCGCCG AAACTAACGT CCCACTGGAC CCGAATGTGC ATCAGGCCAT CGCAATGGTG GAATCTGATG ACGTTGCGCC AGGTAACGTA CTGGGCATTA TGCAGAAGGG TTATACGCTG AATGGTCGTA CGATTCGTGC GGCGATGGTT ACTGTAGCGA AAGCAAAAGC TTAATTTCTG CTTTCGTAAT AATTCACGGC CCTGCATGCA AGCTCAGATC TGAGCTTGGC TGTTTTGGCG GATGAGAGAA GAGGTCGACT CTAGAGGATC TACTAGTCAT AGGCGCCGAT ATCAGATCCG GTACCAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TACAACTTAT ATCGTATGGG GCTGACTTCA GGTGCTACAT TTGAAGAGAT AAATTGCACT GAAATCTAGA AATATTTTAT CTGATTAATA AGATGATCTT CTTGAGATCG TTTTGGTCTG CGCGTAATCT CTTGCTCTGA AAACGAAAAA ACCGCCTTGC AGGGCGGTTT TTCGAAGGTT CTCTGAGCTA CCAACTCTTT GAACCGAGGT AACTGGCTTG GAGGAGCGCA GTCACCAAAA CTTGTCCTTT CAGTTTAGCC TTAACCGGCG CATGACTTCA AGACTAACTC CTCTAAATCA ATTACCAGTG GCTGCTGCCA GTGGTGCTTT TGCATGTCTT TCCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGA CTGAACGGGG GGTTCGTGCA TACAGTCCAG CTTGGAGCGA ACTGCCTACC CGGAACTGAG TGTCAGGCGT GGAATGAGAC AAACGCGGCC ATAACAGCGG AATGACACCG GTAAACCGAA AGGCAGGAAC AGGAGAGCGC ACGAGGGAGC CGCCAGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC ACTGATTTGA GCGTCAGATT TCGTGATGCT TGTCAGGGGG GCGGAGCCTA TGGAAAAACG GCTTTGCCGC GGCCCTCTCA CTTCCCTGTT AAGTATCTTC CTGGCATCTT CCAGGAAATC TCCGCCCCGT TCGTAAGCCA TTTCCGCTCG CCGCAGTCGA ACGACCGAGC GTAGCGAGTC AGTGAGCGAG GAAGCGGAAT ATATCCTGTA TCACATATTC TGCTGACGCA CCGGTGCAGC CTTTTTTCTC CTGCCACATG AAGCACTTCA CTGACACCCT CATCAGTGCC AACATAGTAA GCCAGTATAC ACTCCGCTAG CGCTGATGTC CGGCGGTGCT TTTGCCGTTA CGCACCACCC CGTCAGTAGC TGAACAGGAG GGACAGCTGA TAGAAACAGA AGCCACTGGA GCACCTCAAA AACACCATCA TACACTAAAT CAGTAAGTTG GCAGCATCAC CCGACGCACT TTGCGCCGAA TAAATACCTG TGACGGAAGA TCACTTCGCA GAATAAATAA ATCCTGGTGT CCCTGTTGAT ACCGGGAAGC CCTGGGCCAA CTTTTGGCGA AAATGAGACG TTGATCGGCA CGTAAGAGGT TCCAACTTTC ACCATAATGA AATAAGATCA CTACCGGGCG TATTTTTTGA GTTATCGAGA TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTC
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