pG-KJE8
$99.00
In stock
SKU
PL82737
分子伴侣质粒
100μg liquid plasmid(Instantly):$120
| name | pG-KJE8 | another name | apG-KJE8 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | p0484-F：GGTAGCTCAGAGAACCTTCG | |
| 3′ sequencing primer | p0484-R：gaaactaaatctgctggcgg | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AATAAGTGAT AATAAGCGGA TGAATGGCAG AAATTCGAAG AATAGTTACG GCTTATGACA TCTTTGTGGA CACATCATTC ACTTTTTATT CACATCCGGC CCTGAACTCG CTAGGACTTG CCCCGGTGCA TTTTTTAAAT ACCCGCGAAA AATAGAGCTG ATCGTCAAAT CCAACATTGC GCCCAACGGT CGCTATCGGC ATTCGCGTAG TGCTAAGCAG AAGTTTCGCC TGGCTGATAC GCTGATCTTC GCGCCAGCTC AATACGCTAA TGCCTAACTG CTGGCGGAAC AGATGTGATA ACCGGGAGGG CGACAGGCAG ACATGCTGGG CGACGCTGGC GATATCAAAA TGGCTGTCCG CCAGATGGTC GCTGATATAC TGGCAGGCAT CGCGCACACG GCTATCCATC GGCGGGTGCA ACGACTCATT AATTACCGCC ATACGTCTGA GCAACAACTG CTCCAGCAGA TTGATCGCCA GTAGCTCAGA ATAGCGACCT TCCCCTTGCC CGGCGCTGAT GATCTGCCCG AACAGTTCGC TGAAATGCGG CTGGCGCGCC TCGTCCGGGC GGAAAAATCC TGTCTGGGCA AAGATTGTCG GCCAGGTCAG CCACTCCTGC CAGTAGGCGC GAGGCCGGAA ATAAACCCAC TGGTGATACC ACTCGCTGGC GTCCGGATGC CGTCCATAGT GATGAATCTC GCCCGGCGGA AACAATAATA TATCGCCAGG CCGACAGACA AACTGCTCGC CATTATTATT AATGACGCCC TCTCCGCGGA TGGTCAGGTT AAGAATATAT CCCTTCATGC CCAACGGACG ATCGATAAAA AAATCCAGAT ATCCATTCGC TTCAATTGGC GTCAGCCCGG CGACCAGATG GGCATTAAAT GAATATCCCG GCAATAGCGG ATCATTTTGC GTTTCAGCCA TGATTTCTCT ACCCCCCGAT GTTCAGAGAA GAAACAAATT GTCCATATCG ACCAGGACGA CAGAGCTTCC GTCTCCGCAA GACTTTGCGC TTGATGAAAG CACGTATCAA CCCCGCTTGT GAAAAGCGCT TTGTAACAAA AGCGTACAGT TCAGGCGATA AAATTAAGTA ACAGAAGTGT CTATAACTAT GGCTGGAATG TCCACATTGA ATATTTGCAC AGCGTCACAC TTTGCAAAGC ATTAGCATTT TTGTCCATAA GATTAGCGGA TCCTGCCTGA CGGTTTTTGC CGCGACTCTC TATAATTTCT CCATACCTGT TTTTCTGGAT GGAGTAAGAC CATGGCTATG GATTGTGGAG ACGTTTAGAT GGGTAAAATA ATTGGTATCG ACCTGGGTAC TACCAACTCT TGTGTAGCGA TTATGGATGG CACCACTCCT CGCGTGCTGG AGAACGCCGA AGGCGATCGC ACCACGCCTT CTATCATTGC CTATACCCAG GATGGTGAAA CTCTAGTTGG TCAGCCGGCT AAACGTCAGG CAGTGACGAA CCCGCAAAAC ACTCTGTTTG CGATTAAACG CCTGATTGGT CGCCGCTTCC AGGACGAAGA AGTACAGCGT GATGTTTCCA TCATGCCGTT CAAAATTATT GCTGCTGATA ACGGCGACGC ATGGGTCGAA GTTAAAGGCC AGAAAATGGC ACCGCCGCAG ATTTCTGCTG AAGTGCTGAA AAAAATGAAG AAAACCGCTG AAGATTACCT GGGTGAACCG GTAACTGAAG CTGTTATCAC CGTACCGGCA TACTTTAACG ATGCTCAGCG TCAGGCAACC AAAGACGCAG GCCGTATCGC TGGTCTGGAA GTAAAACGTA TCATCAACGA ACCGACCGCA GCTGCGCTGG CTTACGGTCT GGACAAAGGC ACTGGCAACC GTACTATCGC GGTTTATGAC CTGGGTGGTG GTACTTTCGA TATTTCTATT ATCGAAATCG ACGAAGTTGA CGGCGAAAAA ACCTTCGAAG TTCTGGCAAC CAACGGTGAT ACCCACCTGG GGGGTGAAGA CTTCGACAGC CGTCTGATCA ACTATCTGGT TGAAGAATTC AAGAAAGATC AGGGCATTGA CCTGCGCAAC GATCCGCTGG CAATGCAGCG CCTGAAAGAA GCGGCAGAAA AAGCGAAAAT CGAACTGTCT TCCGCTCAGC AGACCGACGT TAACCTGCCA TACATCACTG CAGACGCGAC CGGTCCGAAA CACATGAACA TCAAAGTGAC TCGTGCGAAA CTGGAAAGCC TGGTTGAAGA TCTGGTAAAC CGTTCCATTG AGCCGCTGAA AGTTGCACTG CAGGACGCTG GCCTGTCCGT ATCTGATATC GACGACGTTA TCCTCGTTGG TGGTCAGACT CGTATGCCAA TGGTTCAGAA GAAAGTTGCT GAGTTCTTTG GTAAAGAGCC GCGTAAAGAC GTTAACCCGG ACGAAGCTGT AGCAATCGGT GCTGCTGTTC AGGGTGGTGT TCTGACTGGT GACGTAAAAG ACGTACTGCT GCTGGACGTT ACCCCGCTGT CTCTGGGTAT CGAAACCATG GGCGGTGTGA TGACGACGCT GATCGCGAAA AACACCACTA TCCCGACCAA GCACAGCCAG GTGTTCTCTA CCGCTGAAGA CAACCAGTCT GCGGTAACCA TCCATGTGCT GCAGGGTGAA CGTAAACGTG CGGCTGATAA CAAATCTCTG GGTCAGTTCA ACCTAGATGG TATCAACCCG GCACCGCGCG GCATGCCGCA GATCGAAGTT ACCTTCGATA TCGATGCTGA CGGTATCCTG CACGTTTCCG CGAAAGATAA AAACAGCGGT AAAGAGCAGA AGATCACCAT CAAGGCTTCT TCTGGTCTGA ACGAAGATGA AATCCAGAAA ATGGTACGCG ACGCAGAAGC TAACGCCGAA GCTGACCGTA AGTTTGAAGA GCTGGTACAG ACTCGCAACC AGGGCGACCA TCTGCTGCAC AGCACCCGTA AGCAGGTTGA AGAAGCAGGC GACAAACTGC CGGCTGACGA CAAAACTGCT ATCGAGTCTG CGCTGACTGC ACTGGAAACT GCTCTGAAAG GTGAAGACAA AGCCGCTATC GAAGCGAAAA TGCAGGAACT GGCACAGGTT TCCCAGAAAC TGATGGAAAT CGCCCAGCAG CAACATGCCC AGCAGCAGAC TGCCGGTGCT GATGCTTCTG CAAACAACGC GAAAGATGAC GATGTTGTCG ACGCTGAATT TGAAGAAGTC AAAGACAAAA AATAATCGCC CTATAAACGG GTAATTATAC TGACACGGGC GAAGGGGAAT TTCCTCCCCG CCCGTGCATT CATCTAGGGG CAATTTAAAA AAGATGGCTA AGCAAGATTA TTACGAGATT TTAGGCGTTT CCAAAACAGC GGAAGAGCGT GAAATCAGAA AGGCCTACAA ACGCCTGGCC ATGAAATACC ACCCGGACCG TAACCAGGGT GACAAAGAGG CCGAGGCGAA ATTTAAAGAG ATCAAGGAAG CTTATGAAGT TCTGACCGAC TCGCAAAAAC GTGCGGCATA CGATCAGTAT GGTCATGCTG CGTTTGAGCA AGGTGGCATG GGCGGCGGCG GTTCTGGCGG CGGCGCAGAC TTCAGCGATA TTTTTGGTGA CGTTTTCGGC GATATTTTTG GCGGCGGACG TGGTCGTCAA CGTGCGGCGC GCGGTGCTGA TTTACGCTAT AACATGGAGC TCACCCTCGA AGAAGCTGTA CGTGGCGTGA CCAAAGAGAT CCGCATTCCG ACTCTGGAAG AGTGTGACGT TTGCCACGGT AGCGGTGCAA AACCAGGTAC ACAGCCGCAG ACTTGTCCGA CCTGTCATGG TTCTGGTCAG GTGCAGATGC GCCAGGGATT CTTCGCTGTA CAGCAGACCT GTCCACACTG TCAGGGCCGC GGTACGCTGA TCAAAGATCC GTGCAACAAA TGTCATGGTC ATGGTCGTGT TGAGCGCAGC AAAACGCTGT CCGTTAAAAT CCCGGCAGGG GTGGACACTG GAGACCGCAT CCGTCTTGCG GGCGAAGGTG AAGCGGGCGA GCATGGCGCA CCGGCAGGCG ATCTGTACGT TCAGGTTCAG GTTAAACAGC ACCCGATTTT CGAGCGTGAA GGCAACAACC TGTATTGCGA AGTCCCGATC AACTTCGCTA TGGCGGCGCT GGGTGGCGAA ATCGAAGTAC CGACCCTTGA TGGTCGCGTC AAACTGAAAG TGCCTGGCGA AACCCAGACC GGTAAGCTAT TCCGTATGCG CGGTAAAGGC GTCAAGTCTG TCCGCGGTGG CGCGCAGGGT GATTTGCTGT GCCGCGTTGT CGTCGAAACA CCGGTAGGCC TGAACGAAAG GCAGAAACAG CTGCTGCAAG AGCTGCAAGA AAGCTTCGGT GGCCCAACCG GCGAGCACAA CAGCCCGCGC TCAAAGAGCT TCTTTGATGG TGTGAAGAAG TTTTTTGACG ACCTGACCCG CTAACCTCGC GGAGAAATTC ATGAGTAGTA AAGAACAGAA AACGCCTGAG GGGCAAGCCC CGGAAGAAAT TATCATGGAT CAGCACGAAG AGATTGAGGC AGTTGAGCCA GAAGCTTCTG CTGAGCAGGT GGATCCGCGC GATGAAAAAG TTGCGAATCT CGAAGCTCAG CTGGCTGAAG CCCAGACCCG TGAACGTGAC GGCATTTTGC GTGTAAAAGC CGAAATGGAA AACCTGCGTC GTCGTACTGA ACTGGATATT GAAAAAGCCC ACAAATTCGC GCTGGAGAAA TTCATCAACG AATTGCTGCC GGTGATTGAT AGCCTGGATC GTGCGCTGGA AGTGGCTGAT AAAGCTAACC CGGATATGTC TGCGATGGTT GAAGGCATTG AGCTGACGCT GAAGTCGATG CTGGATGTTG TGCGTAAGTT TGGCGTTGAA GTGATCGCCG AAACTAACGT CCCACTGGAC CCGAATGTGC ATCAGGCCAT CGCAATGGTG GAATCTGATG ACGTTGCGCC AGGTAACGTA CTGGGCATTA TGCAGAAGGG TTATACGCTG AATGGTCGTA CGATTCGTGC GGCGATGGTT ACTGTAGCGA AAGCAAAAGC TTAATTTCTG CTTTCGTAAT AATTCACGGC CCTGCATGCA AGCTCAGATC TGAGCTTGGC TGTTTTGGCG GATGAGAGAA GAGGTCGACT CTAGAGGATC TACTAGTCAT AGCGCCGATA TCAGATCTGT TTTCAGCCTG ATACAGATTA AATCAGAACG CAGAAGCGGT CTGATAAAAC AGAATTTGCC TGGCGGCAGT AGCGCGGTGG TCCCACCTGA CCCCATGCCG AACTCAGAAG TGAAACGCCG TAGCGCCGAT GGTAGTGTGG GGTCTCCCCA TGCGAGAGTA GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA AATCCGCCGG GAGCGGATTT GAACGTTGCG AAGCAACGGC CCGGAGGGTG GCGGGCAGGA CGCCCGCCAT AAACTGCCAG GCATCAAATT AAGCAGAAGG CCATCCTGAC GGATGGCCTT TTTGCGTTTC TACAAACTCT TTTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GCAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTAC AACTTATATC GTATGGGGCT GACTTCAGGT GCTACATTTG AAGAGATAAA TTGCACTGAA ATCTAGAAAT ATTTTATCTG ATTAATAAGA TGATCTTCTT GAGATCGTTT TGGTCTGCGC GTAATCTCTT GCTCTGAAAA CGAAAAAACC GCCTTGCAGG GCGGTTTTTC GAAGGTTCTC TGAGCTACCA ACTCTTTGAA CCGAGGTAAC TGGCTTGGAG GAGCGCAGTC ACCAAAACTT GTCCTTTCAG TTTAGCCTTA ACCGGCGCAT GACTTCAAGA CTAACTCCTC TAAATCAATT ACCAGTGGCT GCTGCCAGTG GTGCTTTTGC ATGTCTTTCC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGACTG AACGGGGGGT TCGTGCATAC AGTCCAGCTT GGAGCGAACT GCCTACCCGG AACTGAGTGT CAGGCGTGGA ATGAGACAAA CGCGGCCATA ACAGCGGAAT GACACCGGTA AACCGAAAGG CAGGAACAGG AGAGCGCACG AGGGAGCCGC CAGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCACT GATTTGAGCG TCAGATTTCG TGATGCTTGT CAGGGGGGCG GAGCCTATGG AAAAACGGCT TTGCCGCGGC CCTCTCACTT CCCTGTTAAG TATCTTCCTG GCATCTTCCA GGAAATCTCC GCCCCGTTCG TAAGCCATTT CCGCTCGCCG CAGTCGAACG ACCGAGCGTA GCGAGTCAGT GAGCGAGGAA GCGGAATATA TCCTGTATCA CATATTCTGC TGACGCACCG GTGCAGCCTT TTTTCTCCTG CCACATGAAG CCGGTACCCG GGGATCCGAT CCGTTTCCAT TTAGGTGGGT ACGTTGGAGC CGCATTATTT TCGCTTTATG AATCTAAAGG GTGGTTAACT CGACATCTTG GTTACCGTGA AGTTACCATC ACGGAAAAAG GTTATGCTGC TTTTAAGACC CACTTTCACA TTTAAGTTGT TTTTCTAATC CGCATATGAT CAATTCAAGG CCGAATAAGA AGGCTGGCTC TGCACCTTGG TGATCAAATA ATTCGATAGC TTGTCGTAAT AATGGCGGCA TACTATCAGT AGTAGGTGTT TCCCTTTCTT CTTTAGCGAC TTGATGCTCT TGATCTTCCA ATACGCAACC TAAAGTAAAA TGCCCCACAG CGCTGAGTGC ATATAATGCA TTCTCTAGTG AAAAACCTTG TTGGCATAAA AAGGCTAATT GATTTTCGAG AGTTTCATAC TGTTTTTCTG TAGGCCGTGT ACCTAAATGT ACTTTTGCTC CATCGCGATG ACTTAGTAAA GCACATCTAA AACTTTTAGC GTTATTACGT AAAAAATCTT GCCAGCTTTC CCCTTCTAAA GGGCAAAAGT GAGTATGGTG CCTATCTAAC ATCTCAATGG CTAAGGCGTC GAGCAAAGCC CGCTTATTTT TTACATGCCA ATACAATGTA GGCTGCTCTA CACCTAGCTT CTGGGCGAGT TTACGGGTTG TTAAACCTTC GATTCCGACC TCATTAAGCA GCTCTAATGC GCTGTTAATC ACTTTACTTT TATCTAATCT AGACATCATT AATTCCTAAT TTTTGTTGAC ACTCTATCGT TGATAGAGTT ATTTTACCAC TCCCTATCAG TGATAGAGAA AAGTGAAAAT CCATATGACT AGTAGATCCT CTAGAGTCGA CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTTCAC CTCGAGAAAT CATAAAAAAT TTATTTGCTT CCCTATCAGT GATAGAGTAT AATAGAGTCG AATTGTTAGC GGAGAAGAAT TTCACACAGA ATTCATTAAA GAGGAGAAAG TCGAGGCGTC ACCCATAACA GATACGGACT TTCTCAAAGG AGAGTTATCA ATGAATATTC GTCCATTGCA TGATCGCGTG ATCGTCAAGC GTAAAGAAGT TGAAACTAAA TCTGCTGGCG GCATCGTTCT GACCGGCTCT GCAGCGGCTA AATCCACCCG CGGCGAAGTG CTGGCTGTCG GCAATGGCCG TATCCTTGAA AATGGCGAAG TGAAGCCGCT GGATGTGAAA GTTGGCGACA TCGTTATTTT CAACGATGGC TACGGTGTGA AATCTGAGAA GATCGACAAT GAAGAAGTGT TGATCATGTC CGAAAGCGAC ATTCTGGCAA TTGTTGAAGC GTAATCCGCG CACGACACTG AACATACGAA TTTAAGGAAT AAAGATAATG GCAGCTAAAG ACGTAAAATT CGGTAACGAC GCTCGTGTGA AAATGCTGCG CGGCGTAAAC GTACTGGCAG ATGCAGTGAA AGTTACCCTC GGTCCAAAAG GCCGTAACGT AGTTCTGGAT AAATCTTTCG GTGCACCGAC CATCACCAAA GATGGTGTTT CCGTTGCTCG TGAAATCGAA CTGGAAGACA AGTTCGAAAA TATGGGTGCG CAGATGGTGA AAGAAGTTGC CTCTAAAGCA AACGACGCTG CAGGCGACGG TACCACCACT GCAACCGTAC TGGCTCAGGC TATCATCACT GAAGGTCTGA AAGCTGTTGC TGCGGGCATG AACCCGATGG ACCTGAAACG TGGTATCGAC AAAGCGGTTA CCGCTGCAGT TGAAGAACTG AAAGCGCTGT CCGTACCATG CTCTGACTCT AAAGCGATTG CTCAGGTTGG TACCATCTCC GCTAACTCCG ACGAAACCGT AGGTAAACTG ATCGCTGAAG CGATGGACAA AGTCGGTAAA GAAGGCGTTA TCACCGTTGA AGACGGTACC GGTCTGCAGG ACGAACTGGA CGTGGTTGAA GGTATGCAGT TCGACCGTGG CTACCTGTCT CCTTACTTCA TCAACAAGCC GGAAACTGGC GCAGTAGAAC TGGAAAGCCC GTTCATCCTG CTGGCTGACA AGAAAATCTC CAACATCCGC GAAATGCTGC CGGTTCTGGA AGCTGTTGCC AAAGCAGGCA AACCGCTGCT GATCATCGCT GAAGATGTAG AAGGCGAAGC GCTGGCAACT CTGGTTGTTA ACACCATGCG TGGCATCGTG AAAGTCGCTG CGGTTAAAGC ACCGGGCTTC GGCGATCGTC GTAAAGCTAT GCTGCAGGAT ATCGCAACCC TGACTGGCGG TACCGTGATC TCTGAAGAGA TCGGTATGGA GCTGGAAAAA GCAACCCTGG AAGACCTGGG TCAGGCTAAA CGTGTTGTGA TCAACAAAGA CACCACCACT ATCATCGATG GCGTGGGTGA AGAAGCTGCA ATCCAGGGCC GTGTTGCTCA GATCCGTCAG CAGATTGAAG AAGCAACTTC TGACTACGAC CGTGAAAAAC TGCAGGAACG CGTAGCGAAA CTGGCAGGCG GCGTTGCAGT TATCAAAGTG GGTGCTGCTA CCGAAGTTGA AATGAAAGAG AAAAAAGCAC GCGTTGAAGA TGCCCTGCAC GCGACCCGTG CTGCGGTAGA AGAAGGCGTG GTTGCTGGTG GTGGTGTTGC GCTGATCCGC GTAGCGTCTA AACTGGCTGA CCTGCGTGGT CAGAACGAAG ACCAGAACGT GGGTATCAAA GTTGCACTGC GTGCAATGGA AGCTCCGCTG CGTCAGATCG TATTGAACTG CGGCGAAGAA CCGTCTGTTG TTGCTAACAC CGTTAAAGGC GGCGACGGCA ACTACGGTTA CAACGCAGCA ACCGAAGAAT ACGGCAACAT GATCGACATG GGTATCCTGG ATCCAACCAA AGTAACTCGT TCTGCTCTGC AGTACGCAGC TTCTGTGGCT GGCCTGATGA TCACCACCGA ATGCATGGTT ACCGACCTGC CGAAAAACGA TGCAGCTGAC TTAGGCGCTG CTGGCGGTAT GGGCGGCATG GGTGGCATGG GCGGCATGAT GTAATTGCCC TGCACCTCGC AGAAATAAAC AAACCCCCGG GCAGAAATGT CTGGGGGTTT TTCTTTTGGT CATCTTTCTA GTATAAAGAT TCAGACACGG ACGACGCGAG TGGCGCCTCG ACTAGAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGCCCTAG ACCTAGACTT CACTGACACC CTCATCAGTG CCAACATAGT AAGCCAGTAT ACACTCCGCT AGCGCTGATG TCCGGCGGTG CTTTTGCCGT TACGCACCAC CCCGTCAGTA GCTGAACAGG AGGGACAGCT GATAGAAACA GAAGCCACTG GAGCACCTCA AAAACACCAT CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTC
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