pTf16
$99.00
In stock
SKU
PL82735
分子伴侣质粒
100μg liquid plasmid(Instantly):$120
| name | pTf16 | another name | apTf16 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | P0488-F： | |
| 3′ sequencing primer | p0488-R：GGTAGCTCAGAGAACCTTCG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGTATGGCAA TGAAAGACGG TGAGCTGGTG ATATGGGATA GTGTTCACCC TTGTTACACC GTTTTCCATG AGCAAACTGA AACGTTTTCA TCGCTCTGGA GTGAATACCA CGACGATTTC CGGCAGTTTC TACACATATA TTCGCAAGAT GTGGCGTGTT ACGGTGAAAA CCTGGCCTAT TTCCCTAAAG GGTTTATTGA GAATATGTTT TTCGTCTCAG CCAATCCCTG GGTGAGTTTC ACCAGTTTTG ATTTAAACGT GGCCAATATG GACAACTTCT TCGCCCCCGT TTTCACCATG GGCAAATATT ATACGCAAGG CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA ATTTTTTTAA GGCAGTTATT GGTGCCCTTA AACGCCTGGT GCTACGCCTG AATAAGTGAT AATAAGCGGA TGAATGGCAG AAATTCGAAG AATAGTTACG GCTTATGACA TCTTTGTGGA CACATCATTC ACTTTTTATT CACATCCGGC CCTGAACTCG CTAGGACTTG CCCCGGTGCA TTTTTTAAAT ACCCGCGAAA AATAGAGCTG ATCGTCAAAT CCAACATTGC GCCCAACGGT CGCTATCGGC ATTCGCGTAG TGCTAAGCAG AAGTTTCGCC TGGCTGATAC GCTGATCTTC GCGCCAGCTC AATACGCTAA TGCCTAACTG CTGGCGGAAC AGATGTGATA ACCGGGAGGG CGACAGGCAG ACATGCTGGG CGACGCTGGC GATATCAAAA TGGCTGTCCG CCAGATGGTC GCTGATATAC TGGCAGGCAT CGCGCACACG GCTATCCATC GGCGGGTGCA ACGACTCATT AATTACCGCC ATACGTCTGA GCAACAACTG CTCCAGCAGA TTGATCGCCA GTAGCTCAGA ATAGCGACCT TCCCCTTGCC CGGCGCTGAT GATCTGCCCG AACAGTTCGC TGAAATGCGG CTGGCGCGCC TCGTCCGGGC GGAAAAATCC TGTCTGGGCA AAGATTGTCG GCCAGGTCAG CCACTCCTGC CAGTAGGCGC GAGGCCGGAA ATAAACCCAC TGGTGATACC ACTCGCTGGC GTCCGGATGC CGTCCATAGT GATGAATCTC GCCCGGCGGA AACAATAATA TATCGCCAGG CCGACAGACA AACTGCTCGC CATTATTATT AATGACGCCC TCTCCGCGGA TGGTCAGGTT AAGAATATAT CCCTTCATGC CCAACGGACG ATCGATAAAA AAATCCAGAT ATCCATTCGC TTCAATTGGC GTCAGCCCGG CGACCAGATG GGCATTAAAT GAATATCCCG GCAATAGCGG ATCATTTTGC GTTTCAGCCA TGATTTCTCT ACCCCCCGAT GTTCAGAGAA GAAACAAATT GTCCATATCG ACCAGGACGA CAGAGCTTCC GTCTCCGCAA GACTTTGCGC TTGATGAAAG CACGTATCAA CCCCGCTTGT GAAAAGCGCT TTGTAACAAA AGCGTACAGT TCAGGCGATA AAATTAAGTA ACAGAAGTGT CTATAACTAT GGCTGGAATG TCCACATTGA ATATTTGCAC AGCGTCACAC TTTGCAAAGC ATTAGCATTT TTGTCCATAA GATTAGCGGA TCCTGCCTGA CGGTTTTTGC CGCGACTCTC TATAATTTCT CCATACCTGT TTTTCTGGAT GGAGTAAGAC CATGGCCTTT CGGGATGATT CTGGTAACAG GGAATGTGAT TGATTATAAG AACATCCCGG TTCCGCGAAG CCAACAACCT GTGCTTGCGG GGTAAGAGTT GACCGAGCAC TGTGATTTTT TGAGGTAACA AGATGCAAGT TTCAGTTGAA ACCACTCAAG GCCTTGGCCG CCGTGTAACG ATTACTATCG CTGCTGACAG CATCGAGACC GCTGTTAAAA GCGAGCTGGT CAACGTTGCG AAAAAAGTAC GTATTGACGG CTTCCGCAAA GGCAAAGTGC CAATGAATAT CGTTGCTCAG CGTTATGGCG CGTCTGTACG CCAGGACGTT CTGGGTGACC TGATGAGCCG TAACTTCATT GACGCCATCA TTAAAGAAAA AATCAATCCG GCTGGCGCAC CGACTTATGT TCCGGGCGAA TACAAGCTGG GTGAAGACTT CACTTACTCT GTAGAGTTTG AAGTTTATCC GGAAGTTGAA CTGCAGGGTC TGGAAGCGAT CGAAGTTGAA AAACCGATCG TTGAAGTGAC CGACGCTGAC GTTGACGGCA TGCTGGATAC TCTGCGTAAA CAGCAGGCGA CCTGGAAAGA AAAAGACGGC GCTGTTGAAG CAGAAGACCG CGTAACCATC GACTTCACCG GTTCTGTAGA CGGCGAAGAG TTCGAAGGCG GTAAAGCGTC TGATTTCGTA CTGGCGATGG GCCAGGGTCG TATGATCCCG GGCTTTGAAG ACGGTATCAA AGGCCACAAA GCTGGCGAAG AGTTCACCAT CGACGTGACC TTCCCGGAAG AATACCACGC AGAAAACCTG AAAGGTAAAG CAGCGAAATT CGCTATCAAC CTGAAGAAAG TTGAAGAGCG TGAACTGCCG GAACTGACTG CAGAATTCAT CAAACGTTTC GGCGTTGAAG ATGGTTCCGT AGAAGGTCTG CGCGCTGAAG TGCGTAAAAA CATGGAGCGC GAGCTGAAGA GCGCCATCCG TAACCGCGTT AAGTCTCAGG CGATCGAAGG TCTGGTAAAA GCTAACGACA TCGACGTACC GGCTGCGCTG ATCGACAGCG AAATCGACGT TCTGCGTCGC CAGGCTGCAC AGCGTTTCGG TGGCAACGAA AAACAAGCTC TGGAACTGCC GCGCGAACTG TTCGAAGAAC AGGCTAAACG CCGCGTAGTT GTTGGCCTGC TGCTGGGCGA AGTTATCCGC ACCAACGAGC TGAAAGCTGA CGAAGAGCGC GTGAAAGGCC TGATCGAAGA GATGGCTTCT GCGTACGAAG ATCCGAAAGA AGTTATCGAG TTCTACAGCA AAAACAAAGA ACTGATGGAC AACATGCGCA ATGTTGCTCT GGAAGAACAG GCTGTTGAAG CTGTACTGGC GAAAGCGAAA GTGACTGAAA AAGAAACCAC TTTCAACGAG CTGATGAACC AGCAGGCGTA ATTTACGCAG CATAACGCGC TAAATTCGCA CAAAGGCCCG TCACCGCCAG GTGGTGGGCT TTTTTTTGTC ATGAATTTTG CATGGAACCG TGCGAAAAGC CTCTTTCGGT GTTAGCGTAA CAACAAAAGA TTGTTATGCT TGAAATATGG TGATGCCGTA CCCATAACAC AGGGACTAGC TGATAATCCG TCCATAAGGT TACAATCGGT ACAGCAGGTT TTTTCAATTT TATCCAGGAG ACGGAAATGT CATACAGCGG CGAACGAGAT AACTTTGCAC CCCATAGATC TGGTACCAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTACAACTT ATATCGTATG GGGCTGACTT CAGGTGCTAC ATTTGAAGAG ATAAATTGCA CTGAAATCTA GAAATATTTT ATCTGATTAA TAAGATGATC TTCTTGAGAT CGTTTTGGTC TGCGCGTAAT CTCTTGCTCT GAAAACGAAA AAACCGCCTT GCAGGGCGGT TTTTCGAAGG TTCTCTGAGC TACCAACTCT TTGAACCGAG GTAACTGGCT TGGAGGAGCG CAGTCACCAA AACTTGTCCT TTCAGTTTAG CCTTAACCGG CGCATGACTT CAAGACTAAC TCCTCTAAAT CAATTACCAG TGGCTGCTGC CAGTGGTGCT TTTGCATGTC TTTCCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GACTGAACGG GGGGTTCGTG CATACAGTCC AGCTTGGAGC GAACTGCCTA CCCGGAACTG AGTGTCAGGC GTGGAATGAG ACAAACGCGG CCATAACAGC GGAATGACAC CGGTAAACCG AAAGGCAGGA ACAGGAGAGC GCACGAGGGA GCCGCCAGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCACTGATTT GAGCGTCAGA TTTCGTGATG CTTGTCAGGG GGGCGGAGCC TATGGAAAAA CGGCTTTGCC GCGGCCCTCT CACTTCCCTG TTAAGTATCT TCCTGGCATC TTCCAGGAAA TCTCCGCCCC GTTCGTAAGC CATTTCCGCT CGCCGCAGTC GAACGACCGA GCGTAGCGAG TCAGTGAGCG AGGAAGCGGA ATATATCCTG TATCACATAT TCTGCTGACG CACCGGTGCA GCCTTTTTTC TCCTGCCACA TGAAGCACTT CACTGACACC CTCATCAGTG CCAACATAGT AAGCCAGTAT ACACTCCGCT AGCGCTGATG TCCGGCGGTG CTTTTGCCGT TACGCACCAC CCCGTCAGTA GCTGAACAGG AGGGACAGCT GATAGAAACA GAAGCCACTG GAGCACCTCA AAAACACCAT CATACACTAA ATCAGTAAGT TGGCAGCATC ACCCGACGCA CTTTGCGCCG AATAAATACC TGTGACGGAA GATCACTTCG CAGAATAAAT AAATCCTGGT GTCCCTGTTG ATACCGGGAA GCCCTGGGCC AACTTTTGGC GAAAATGAGA CGTTGATCGG CACGTAAGAG GTTCCAACTT TCACCATAAT GAAATAAGAT CACTACCGGG CGTATTTTTT GAGTTATCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG AGAAAAAAAT CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT TTGAGGCATT TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA CGGCCTTTTT AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA TTCTTGCCCG CCTGATGAAT GCTCATCCGG AATTC
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