pGro7
$99.00
In stock
SKU
PL82727
分子伴侣质粒
100μg liquid plasmid(Instantly):$134
| name | pGro7 | another name | apGro7 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | Fluorescent labeling | ||
| Promoter | araBAD | Replicon | p15A |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | P0486-F：aatgcccatctggtcgc | |
| 3′ sequencing primer | p0486-R：GGTAGCTCAGAGAACCTTCG | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCTG GCGGCTCCCT CGTGCGCTCT CCTGTTCCTG CCTTTCGGTT TACCGGTGTC ATTCCGCTGT TATGGCCGCG TTTGTCTCAT TCCACGCCTG ACACTCAGTT CCGGGTAGGC AGTTCGCTCC AAGCTGGACT GTATGCACGA ACCCCCCGTT CAGTCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGAAAGACAT GCAAAAGCAC CACTGGCAGC AGCCACTGGT AATTGATTTA GAGGAGTTAG TCTTGAAGTC ATGCGCCGGT TAAGGCTAAA CTGAAAGGAC AAGTTTTGGT GACTGCGCTC CTCCAAGCCA GTTACCTCGG TTCAAAGAGT TGGTAGCTCA GAGAACCTTC GAAAAACCGC CCTGCAAGGC GGTTTTTTCG TTTTCAGAGC AAGAGATTAC GCGCAGACCA AAACGATCTC AAGAAGATCA TCTTATTAAT CAGATAAATA TTTCTAGATT TCAGTGCAAT TTATCTCTTC AAATGTAGCA CCTGAAGTCA GCCCCATACG ATATAAGTTG TAATTCTCAT GTTTGACAGC TTATCATCGA TAAGCTTGGT ACCAGATCTG ATATCGGCGC CTCGAGGCGC CACTCGCGTC GTCCGTGTCT GAATCTTATA CTAGAAAGAT GACCAAAAGA AAAACCCCCA GACATTTCTG CCCGGGGGTT TGTTTATTTC TGCGAGGTGC AGGGCAATTA CATCATGCCG CCCATGCCAC CCATGCCGCC CATACCGCCA GCAGCGCCTA AGTCAGCTGC ATCGTTTTTC GGCAGGTCGG TAACCATGCA TTCGGTGGTG ATCATCAGGC CAGCCACAGA AGCTGCGTAC TGCAGAGCAG AACGAGTTAC TTTGGTTGGA TCCAGGATAC CCATGTCGAT CATGTTGCCG TATTCTTCGG TTGCTGCGTT GTAACCGTAG TTGCCGTCGC CGCCTTTAAC GGTGTTAGCA ACAACAGACG GTTCTTCGCC GCAGTTCAAT ACGATCTGAC GCAGCGGAGC TTCCATTGCA CGCAGTGCAA CTTTGATACC CACGTTCTGG TCTTCGTTCT GACCACGCAG GTCAGCCAGT TTAGACGCTA CGCGGATCAG CGCAACACCA CCACCAGCAA CCACGCCTTC TTCTACCGCA GCACGGGTCG CGTGCAGGGC ATCTTCAACG CGTGCTTTTT TCTCTTTCAT TTCAACTTCG GTAGCAGCAC CCACTTTGAT AACTGCAACG CCGCCTGCCA GTTTCGCTAC GCGTTCCTGC AGTTTTTCAC GGTCGTAGTC AGAAGTTGCT TCTTCAATCT GCTGACGGAT CTGAGCAACA CGGCCCTGGA TTGCAGCTTC TTCACCCACG CCATCGATGA TAGTGGTGGT GTCTTTGTTG ATCACAACAC GTTTAGCCTG ACCCAGGTCT TCCAGGGTTG CTTTTTCCAG CTCCATACCG ATCTCTTCAG AGATCACGGT ACCGCCAGTC AGGGTTGCGA TATCCTGCAG CATAGCTTTA CGACGATCGC CGAAGCCCGG TGCTTTAACC GCAGCGACTT TCACGATGCC ACGCATGGTG TTAACAACCA GAGTTGCCAG CGCTTCGCCT TCTACATCTT CAGCGATGAT CAGCAGCGGT TTGCCTGCTT TGGCAACAGC TTCCAGAACC GGCAGCATTT CGCGGATGTT GGAGATTTTC TTGTCAGCCA GCAGGATGAA CGGGCTTTCC AGTTCTACTG CGCCAGTTTC CGGCTTGTTG ATGAAGTAAG GAGACAGGTA GCCACGGTCG AACTGCATAC CTTCAACCAC GTCCAGTTCG TCCTGCAGAC CGGTACCGTC TTCAACGGTG ATAACGCCTT CTTTACCGAC TTTGTCCATC GCTTCAGCGA TCAGTTTACC TACGGTTTCG TCGGAGTTAG CGGAGATGGT ACCAACCTGA GCAATCGCTT TAGAGTCAGA GCATGGTACG GACAGCGCTT TCAGTTCTTC AACTGCAGCG GTAACCGCTT TGTCGATACC ACGTTTCAGG TCCATCGGGT TCATGCCCGC AGCAACAGCT TTCAGACCTT CAGTGATGAT AGCCTGAGCC AGTACGGTTG CAGTGGTGGT ACCGTCGCCT GCAGCGTCGT TTGCTTTAGA GGCAACTTCT TTCACCATCT GCGCACCCAT ATTTTCGAAC TTGTCTTCCA GTTCGATTTC ACGAGCAACG GAAACACCAT CTTTGGTGAT GGTCGGTGCA CCGAAAGATT TATCCAGAAC TACGTTACGG CCTTTTGGAC CGAGGGTAAC TTTCACTGCA TCTGCCAGTA CGTTTACGCC GCGCAGCATT TTCACACGAG CGTCGTTACC GAATTTTACG TCTTTAGCTG CCATTATCTT TATTCCTTAA ATTCGTATGT TCAGTGTCGT GCGCGGATTA CGCTTCAACA ATTGCCAGAA TGTCGCTTTC GGACATGATC AACACTTCTT CATTGTCGAT CTTCTCAGAT TTCACACCGT AGCCATCGTT GAAAATAACG ATGTCGCCAA CTTTCACATC CAGCGGCTTC ACTTCGCCAT TTTCAAGGAT ACGGCCATTG CCGACAGCCA GCACTTCGCC GCGGGTGGAT TTAGCCGCTG CAGAGCCGGT CAGAACGATG CCGCCAGCAG ATTTAGTTTC AACTTCTTTA CGCTTGACGA TCACGCGATC ATGCAATGGA CGAATATTCA TTGATAACTC TCCTTTGAGA AAGTCCGTAT CTGTTATGGG TGACGCCTCG AGCCATGGTC TTACTCCATC CAGAAAAACA GGTATGGAGA AATTATAGAG AGTCGCGGCA AAAACCGTCA GGCAGGATCC GCTAATCTTA TGGACAAAAA TGCTAATGCT TTGCAAAGTG TGACGCTGTG CAAATATTCA ATGTGGACAT TCCAGCCATA GTTATAGACA CTTCTGTTAC TTAATTTTAT CGCCTGAACT GTACGCTTTT GTTACAAAGC GCTTTTCACA AGCGGGGTTG ATACGTGCTT TCATCAAGCG CAAAGTCTTG CGGAGACGGA AGCTCTGTCG TCCTGGTCGA TATGGACAAT TTGTTTCTTC TCTGAACATC GGGGGGTAGA GAAATCATGG CTGAAACGCA AAATGATCCG CTATTGCCGG GATATTCATT TAATGCCCAT CTGGTCGCCG GGCTGACGCC AATTGAAGCG AATGGATATC TGGATTTTTT TATCGATCGT CCGTTGGGCA TGAAGGGATA TATTCTTAAC CTGACCATCC GCGGAGAGGG CGTCATTAAT AATAATGGCG AGCAGTTTGT CTGTCGGCCT GGCGATATAT TATTGTTTCC GCCGGGCGAG ATTCATCACT ATGGACGGCA TCCGGACGCC AGCGAGTGGT ATCACCAGTG GGTTTATTTC CGGCCTCGCG CCTACTGGCA GGAGTGGCTG ACCTGGCCGA CAATCTTTGC CCAGACAGGA TTTTTCCGCC CGGACGAGGC GCGCCAGCCG CATTTCAGCG AACTGTTCGG GCAGATCATC AGCGCCGGGC AAGGGGAAGG TCGCTATTCT GAGCTACTGG CGATCAATCT GCTGGAGCAG TTGTTGCTCA GACGTATGGC GGTAATTAAT GAGTCGTTGC ACCCGCCGAT GGATAGCCGT GTGCGCGATG CCTGCCAGTA TATCAGCGAC CATCTGGCGG ACAGCCATTT TGATATCGCC AGCGTCGCCC AGCATGTCTG CCTGTCGCCC TCCCGGTTAT CACATCTGTT CCGCCAGCAG TTAGGCATTA GCGTATTGAG CTGGCGCGAA GATCAGCGTA TCAGCCAGGC GAAACTTCTG CTTAGCACTA CGCGAATGCC GATAGCGACC GTTGGGCGCA ATGTTGGATT TGACGATCAG CTCTATTTTT CGCGGGTATT TAAAAAATGC ACCGGGGCAA GTCCTAGCGA GTTCAGGGCC GGATGTGAAT AAAAAGTGAA TGATGTGTCC ACAAAGATGT CATAAGCCGT AACTATTCTT CGAATTTCTG CCATTCATCC GCTTATTATC ACTTATTCAG GCGTAGCACC AGGCGTTTAA GGGCACCAAT AACTGCCTTA AAAAAATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AGACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACG
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