pSoup
$128.00
In stock
SKU
PL82695
100μg liquid plasmid(Instantly):$149
| name | pSoup | another name | apSoup |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | packaging aid | Fragment type | |
| Fragment species | Prokaryotic resistance | Tet | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pVS | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | 35s： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCCCTTTCCA CCGCGTCAGA CGCCCGTAGC AGCCCGCTAC GGGCTTTTTC ATGCCCTGCC CTAGCGTCCA AGCCTCACGG CCGCGCTCGG CCTCTCTGGC GGCCTTCTGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTTCCG CTGCATAACC CTGCTTCGGG GTCATTATAG CGATTTTTTC GGTATATCCA TCCTTTTTCG CACGATATAC AGGATTTTGC CAAAGGGTTC GTGTAGACTT TCCTTGGTGT ATCCAACGGC GTCAGCCGGG CAGGATAGGT GAAGTAGGCC CACCCGCGAG CGGGTGTTCC TTCTTCACTG TCCCTTATTC GCACCTGGCG GTGCTCAACG GGAATCCTGC TCTGCGAGGC TGGCCGGCTA CCGCCGGCGT AACAGATGAG GGCAAGCGGA TGGCTGATGA AACCAAGCCA ACCAGGAAGG GCAGCCCACC TATCAAGGTG TACTGCCTTC CAGACGAACG AAGAGCGATT GAGGAAAAGG CGGCGGCGGC CGGCATGAGC CTGTCGGCCT ACCTGCTGGC CGTCGGCCAG GGCTACAAAA TCACGGGCGT CGTGGACTAT GAGCACGTCC GCGAGCTGGC CCGCATCAAT GGCGACCTGG GCCGCCTGGG CGGCCTGCTG AAACTCTGGC TCACCGACGA CCCGCGCACG GCGCGGTTCG GTGATGCCAC GATCCTCGCC CTGCTGGCGA AGATCGAAGA GAAGCAGGAC GAGCTTGGCA AGGTCATGAT GGGCGTGGTC CGCCCGAGGG CAGAGCCATG ACTTTTTTAG CCGCTAAAAC GGCCGGGGGG TGCGCGTGAT TGCCAAGCAC GTCCCCATGC GCTCCATCAA GAAGAGCGAC TTCGCGGAGC TGGTGAAGTA CATCACCGAC GAGCAAGGCA AGACCGAGCG CCTTTGCGAC GCTCACCGGG CTGGTTGCCC TCGCCGCTGG GCTGGCGGCC GTCTATGGCC CTGCAAACGC GCCAGAAACG CCGTCGAAGC CGTGTGCGAG ACACCGCGGC CGCCGGCGTT GTGGATACCT CGCGGAAAAC TTGGCCCTCA CTGACAGATG AGGGGCGGAC GTTGACACTT GAGGGGCCGA CTCACCCGGC GCGGCGTTGA CAGATGAGGG GCAGGCTCGA TTTCGGCCGG CGACGTGGAG CTGGCCAGCC TCGCAAATCG GCGAAAACGC CTGATTTTAC GCGAGTTTCC CACAGATGAT GTGGACAAGC CTGGGGATAA GTGCCCTGCG GTATTGACAC TTGAGGGGCG CGACTACTGA CAGATGAGGG GCGCGATCCT TGACACTTGA GGGGCAGAGT GCTGACAGAT GAGGGGCGCA CCTATTGACA TTTGAGGGGC TGTCCACAGG CAGAAAATCC AGCATTTGCA AGGGTTTCCG CCCGTTTTTC GGCCACCGCT AACCTGTCTT TTAACCTGCT TTTAAACCAA TATTTATAAA CCTTGTTTTT AACCAGGGCT GCGCCCTGTG CGCGTGACCG CGCACGCCGA AGGGGGGTGC CCCCCCTTCT CGAACCCTCC CGGCCCGCTA ACGCGGGCCT CCCATCCCCC CAGGGGCTGC GCCCCTCGGC CGCGAACGGC CTCACCCCAA AAATGGCAGC GCTGGCAGTC CTTGCCATTG CCGGGATCGG GGCAGTAACG GGATGGGCGA TCAGCCCGAG CGCGACGCCC GGAAGCATTG ACGTGCCGCA GGTGCTGGCA TCGACATTCA GCGACCAGGT GCCGGGCAGT GAGGGCGGCG GCCTGGGTGG CGGCCTGCCC TTCACTTCGG CCGTCGGGGC ATTCACGGAC TTCATGGCGG GGCCGGCAAT TTTTACCTTG GGCATTCTTG GCATAGTGGT CGCGGGTGCC GTGCTCGTGT TCGGGGGTGC GATAAACCCA GCGAACCATT TGAGGTGATA GGTAAGATTA TACCGAGGTA TGAAAACGAG AATTGGACCT TTACAGAATT ACTCTATGAA GCGCCATATT TAAAAAGCTA CCAAGACGAA GAGGATGAAG AGGATGAGGA GGCAGATTGC CTTGAATATA TTGACAATAC TGATAAGATA ATATATCTTT TATATAGAAG ATAATTCGAG CTCGGTACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTTGA GTATTCTATA GTGTCACCTA AATAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGAATT AATTCTCATG TTTGACAGCT TATCATCGAT TAGCTTTAAT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGGC ACCGTGTATG AAATCTAACA ATGCGCTCAT CGTCATCCTC GGCACCGTCA CCCTGGATGC TGTAGGCATA GGCTTGGTTA TGCCGGTACT GCCGGGCCTC TTGCGGGATA TCGTCCATTC CGACAGCATC GCCAGTCACT ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC GTTCTCGGAG CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT GGAGCCACTA TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT TCTCTACGCC GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTACATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA GCGCCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC GCCGGCATGG CGGCCGACGC GCTGGGCTAC GTCTTGCTGG CGTTCGCGAC GCGAGGCTGG ATGGCCTTCC CCATTATGAT TCTTCTCGCT TCCGGCGGCA TCGGGATGCC CGCGTTGCAG GCCATGCTGT CCAGGCAGGT AGATGACGAC CATCAGGGAC AGCTTCAAGG ATCGCTCGCG GCTCTTACCA GCCTAACTTC GATCACTGGA CCGCTGATCG TCACGGCGAT TTATGCCGCC TCGGCGAGCA CATGGAACGG GTTGGCATGG ATTGTAGGCG CCGCCCTATA CCTTGTCTGC CTCCCCGCGT TGCGTCGCGG TGCATGGAGC CGGGCCACCT CGACCTGAAT GGAAGCCGGC GGCACCTCGC TAACGGATTC ACCACTCCAA GAATTGGAGC CAATCAATTC TTGCGGAGAA CTGTGAATGC GCAAACCAAC CCTTGTATCG GGGAAGAACA GTATGTCGAG CTATTTTTTG ACTTACTGGG GATCAAGCCT GATTGGGAGA AAATAAAATA TTATATTTTA CTGGATGAAT TGTTTTAGTA CCTAGATGTG GCGCAACGAT GCCGGCGACA AGCAGGAGCG CACCGACTTC TTCCGCATCA AGTGTTTTGG CTCTCAGGCC GAGGCCCACG GCAAGTATTT GGGCAAGGGG TCGCTGGTAT TCGTGCAGGG CAAGATTCGG AATACCAAGT ACGAGAAGGA CGGCCAGACG GTCTACGGGA CCGACTTCAT TGCCGATAAG GTGGATTATC TGGACACCAA GGCACCAGGC GGGTCAAATC AGGAATAAGG GCACATTGCC CCGGCGTGAG TCGGGGCAAT CCCGCAAGGA GGGTGAATGA ATCGGACGTT TGACCGGAAG GCATACAGGC AAGAACTGAT CGACGCGGGG TTTTCCGCCG AGGATGCCGA AACCATCGCA AGCCGCACCG TCATGCGTGC GCCCCGCGAA ACCTTCCAGT CCGTCGGCTC GATGGTCCAG CAAGCTACGG CCAAGATCGA GCGCGACAGC GTGCAACTGG CTCCCCCTGC CCTGCCCGCG CCATCGGCCG CCGTGGAGCG TTCGCGTCGT CTCGAACAGG AGGCGGCAGG TTTGGCGAAG TCGATGACCA TCGACACGCG AGGAACTATG ACGACCAAGA AGCGAAAAAC CGCCGGCGAG GACCTGGCAA AACAGGTCAG CGAGGCCAAG CAGGCCGCGT TGCTGAAACA CACGAAGCAG CAGATCAAGG AAATGCAGCT TTCCTTGTTC GATATTGCGC CGTGGCCGGA CACGATGCGA GCGATGCCAA ACGACACGGC CCGCTCTGCC CTGTTCACCA CGCGCAACAA GAAAATCCCG CGCGAGGCGC TGCAAAACAA GGTCATTTTC CACGTCAACA AGGACGTGAA GATCACCTAC ACCGGCGTCG AGCTGCGGGC CGACGATGAC GAACTGGTGT GGCAGCAGGT GTTGGAGTAC GCGAAGCGCA CCCCTATCGG CGAGCCGATC ACCTTCACGT TCTACGAGCT TTGCCAGGAC CTGGGCTGGT CGATCAATGG CCGGTATTAC ACGAAGGCCG AGGAATGCCT GTCGCGCCTA CAGGCGACGG CGATGGGCTT CACGTCCGAC CGCGTTGGGC ACCTGGAATC GGTGTCGCTG CTGCACCGCT TCCGCGTCCT GGACCGTGGC AAGAAAACGT CCCGTTGCCA GGTCCTGATC GACGAGGAAA TCGTCGTGCT GTTTGCTGGC GACCACTACA CGAAATTCAT ATGGGAGAAG TACCGCAAGC TGTCGCCGAC GGCCCGACGG ATGTTCGACT ATTTCAGCTC GCACCGGGAG CCGTACCCGC TCAAGCTGGA AACCTTCCGC CTCATGTGCG GATCGGATTC CACCCGCGTG AAGAAGTGGC GCGAGCAGGT CGGCGAAGCC TGCGAAGAGT TGCGAGGCAG CGGCCTGGTG GAACACGCCT GGGTCAATGA TGACCTGGTG CATTGCAAAC GCTAGGGCCT TGTGGGGTCA GTTCCGGCTG GGGGTTCAGC AGCCAGCGCT TTACTGGCAT TTCAGGAACA AGCGGGCACT GCTCGACGCA CTTGCTTCGC TCAGTATCGC TCGGGACGCA CGGCGCGCTC TACGAACTGC CGATAAACAG AGGATTAAAA TTGACAATTG TGATTAAGGC TCAGATTCGA CGGCTTGGAG CGGCCGACGT GCAGGATTTC CGCGAGATCC GATTGTCGGC CCTGAAGAAA GCTCCAGAGA TGTTCGGGTC CGTTTACGAG CACGAGGAGA AAAAGCCCAT GGAGGCGTTC GCTGAACGGT TGCGAGATGC CGTGGCATTC GGCGCCTACA TCGACGGCGA GATCATTGGG CTGTCGGTCT TCAAACAGGA GGACGGCCCC AAGGACGCTC ACAAGGCGCA TCTGTCCGGC GTTTTCGTGG AGCCCGAACA GCGAGGCCGA GGGGTCGCCG GTATGCTGCT GCGGGCGTTG CCGGCGGGTT TATTGCTCGT GATGATCGTC CGACAGATTC CAACGGGAAT CTGGTGGATG CGCATCTTCA TCCTCGGCGC ACTTAATATT TCGCTATTCT GGAGCTTGTT GTTTATTTCG GTCTACCGCC TGCCGGGCGG GGTCGCGGCG ACGGTAGGCG CTGTGCAGCC GCTGATGGTC GTGTTCATCT CTGCCGCTCT GCTAGGTAGC CCGATACGAT TGATGGCGGT CCTGGGGGCT ATTTGCGGAA CTGCGGGCGT GGCGCTGTTG GTGTTGACAC CAAACGCAGC GCTAGATCCT GTCGGCGTCG CAGCGGGCCT GGCGGGGGCG GTTTCCATGG CGTTCGGAAC CGTGCTGACC CGCAAGTGGC AACCTCCCGT GCCTCTGCTC ACCTTTACCG CCTGGCAACT GGCGGCCGGA GGACTTCTGC TCGTTCCAGT AGCTTTAGTG TTTGATCCGC CAATCCCGAT GCCTACAGGA ACCAATGTTC TCGGCCTGGC GTGGCTCGGC CTGATCGGAG CGGGTTTAAC CTACTTCCTT TGGTTCCGGG GGATCTCGCG ACTCGAACCT ACAGTTGTTT CCTTACTGGG CTTTCTCAGC CCCAGATCCT AGAGGATCGA GCGGCATTTC ATTCAGGCAC GTACCACCGA GGCGCTACAA GTCGCCAGAG AGCGCGGCAA GACGCTCGGG CGACCCAAGG GCAGCAAATC GAGCGCCTTG AAGCTGGACA GCCGTATTGA TGAAGTACAG GCATACGTGA ACCTTGGCTT GCCGCAAAGT CGCGCAGCCG AGTTGTTAGG CGTCAGCCCT CACACCTTGC GCCTGTTCAT CAAACGCCGG AACATCAAAC CCACAAACAC TAGACCAACC ATCACCATGC CGGGGAGGGA ACAACATGCC TAAGAACAAC AAAGCCCCCG GCCATCGTAT CAACGAGATC ATCAAGACGA GCCTCGCGCT CGAAATGGAG GATGCCCGCG AAGCTGGCTT AGTCGGCTAC ATGGCCCGTT GCCTTGTGCA AGCGACCATG CCCCACACCG ACCCCAAGAC CAGCTACTTT GAGCGCACCA ATGGCATCGT CACCTTGTCG ATCATGGGCA AGCCGAGCAT CGGCCTGCCC TACGGTTCTA TGCCGCGCAC CTTGCTTGCT TGGATATGCA CCGAGGCCGT GCGAACGAAA GACCCCGTGT TGAACCTTGG CCGGTCGCAA TCGGAATTTC TACAAAGGCT CGGAATGCAC ACCGATGGCC GTTACACGGC CACCCTTCGC AATCAGGCGC AACGCCTGTT TTCATCCATG ATTTCGCTTG CCGGCGAGCA AGGCAATGAC TTCGGCATTG AGAACGTCGT CATTGCCAAG CGCGCTTTTC TATTCTGGAA TCCCAAGCGG CCAGAAGATC GGGCGCTATG GGATAGCACC CTCACCCTCA CAGGCGATTT CTTCGAGGAA GTCACCCGCT CACCGGTTCC TATCCGAATC GACTACCTGC ACGCCTTGCG GCAGTCTCCG CTTGCGATGG ACATTTACAC GTGGCTGACC TATCGCGTGT TCCTGTTGCG GGCCAAGGGC CGCCCCTTCG TGCAAATCCC TTGGGTCGCC CTGCAAGCGC AATTCGGCTC ATCCTATGGC AGCCGCGCAC GCAACTCGCC CGAACTGGAC GATAAGGCCC GAGAGCGGGC AGAGCGGGCA GCACTCGCCA GCTTCAAATA CAACTTCAAA AAGCGCCTAC GCGAAGTGTT GATTGTCTAT CCCGAGGCAA GCGACTGCAT CGAAGATGAC GGCGAATGCC TGCGCATCAA ATCCACACGC CTGCATGTCA CCCGCGCACC CGGCAAGGGC GCTCGCATCG GCCCCCCTCC GACTTGACCA GGCCAACGCT ACGCTTGGCT TGGTCAAGGA TCGATCTGGC GCCGGCCAGC GAGACGAGCA AGATTGGCCG CCGCCCGAAA CGATCCGACA GCGCGCCCAG CACAGGTGCG CAGGCAAATT GCACCAACGC ATACAGCGCC AGCAGAATGC CATAGTGGGC GGTGACGTCG TTCGAGTGAA CCAGATCGCG CAGGAGGCCC GGCAGCACCG GCATAATCAG GCCGATGCCG ACAGCGTCGA GCGCGACAGT GCTCAGAATT ACGATCAGGG GTATGTTGGG TTTCACGTCT GGCCTCCGGA CCAGCCTCCG CTGGTCCGAT TGAACGCGCG GATTCTTTAT CACTGATAAG TTGGTGGACA TATTATGTTT ATCAGTGATA AAGTGTCAAG CATGACAAAG TTGCAGCCGA ATACAGTGAT CCGTGCCGCC CTGGACCTGT TGAACGAGGT CGGCGTAGAC GGTCTGACGA CACGCAAACT GGCGGAACGG TTGGGGGTTC AGCAGCCGGC GCTTTACTGG CACTTCAGGA ACAAGCGGGC GCTGCTCGAC GCACTGGCCG AAGCCATGCT GGCGGAGAAT CATACGCATT CGGTGCCGAG AGCCGACGAC GACTGGCGCT CATTTCTGAT CGGGAATGCC CGCAGCTTCA GGCAGGCGCT GCTCGCCTAC CGCGATGGCG CGCGCATCCA TGCCGGCACG CGACCGGGCG CACCGCAGAT GGAAACGGCC GACGCGCAGC TTCGCTTCCT CTGCGAGGCG GGTTTTTCGG CCGGGGACGC CGTCAATGCG CTGATGACAA TCAGCTACTT CACTGTTGGG GCCGTGCTTG AGGAGCAGGC CGGCGACAGC GATGCCGGCG AGCGCGGCGG CACCGTTGAA CAGGCTCCGC TCTCGCCGCT GTTGCGGGCC GCGATAGACG CCTTCGACGA AGCCGGTCCG GACGCAGCGT TCGAGCAGGG ACTCGCGGTG ATTGTCGATG GATTGGCGAA AAGGAGGCTC GTTGTCAGGA ACGTTGAAGG ACCGAGAAAG GGTGACGATT GATCAGGACC GCTGCCGGAG CGCAACCCAC TCACTACAGC AGAGCCATGT AGACAACATC CCCTC
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
